AU2001252604A1 - Method of screening drug acting on cell wall - Google Patents
Method of screening drug acting on cell wallInfo
- Publication number
- AU2001252604A1 AU2001252604A1 AU2001252604A AU5260401A AU2001252604A1 AU 2001252604 A1 AU2001252604 A1 AU 2001252604A1 AU 2001252604 A AU2001252604 A AU 2001252604A AU 5260401 A AU5260401 A AU 5260401A AU 2001252604 A1 AU2001252604 A1 AU 2001252604A1
- Authority
- AU
- Australia
- Prior art keywords
- cell wall
- reporter protein
- microorganism
- culture fluid
- released
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 210000002421 cell wall Anatomy 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 238000012216 screening Methods 0.000 title abstract 2
- 239000003814 drug Substances 0.000 title 1
- 229940079593 drug Drugs 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 244000005700 microbiome Species 0.000 abstract 4
- 239000012531 culture fluid Substances 0.000 abstract 3
- 210000000170 cell membrane Anatomy 0.000 abstract 2
- 102000002702 GPI-Linked Proteins Human genes 0.000 abstract 1
- 108010043685 GPI-Linked Proteins Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A method for a screening of an agent acting on a cell wall, which comprises the steps of (1) culturing each of microorganisms having a reporter protein fixed on a cell wall or a cell membrane as a GPI-anchored protein in the presence of a test agent; (2) determining the reporter protein released into each culture fluid of the microorganism cultured; and (3) judging that the test agent has a selective inhibitory action on the cell wall when the reporter protein is released from the microorganism having the reporter protein fixed on the cell wall into the culture fluid and the reporter protein is not substantially released from the microorganism having the reporter protein fixed on the cell membrane into the culture fluid.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000-132041 | 2000-05-01 | ||
| JP2000132041 | 2000-05-01 | ||
| PCT/JP2001/003630 WO2001083733A1 (en) | 2000-05-01 | 2001-04-26 | Method of screening drug acting on cell wall |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001252604A1 true AU2001252604A1 (en) | 2001-11-12 |
Family
ID=18640818
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001252604A Abandoned AU2001252604A1 (en) | 2000-05-01 | 2001-04-26 | Method of screening drug acting on cell wall |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20040091949A1 (en) |
| EP (1) | EP1283261B1 (en) |
| AT (1) | ATE403717T1 (en) |
| AU (1) | AU2001252604A1 (en) |
| CA (1) | CA2407906A1 (en) |
| DE (1) | DE60135215D1 (en) |
| ES (1) | ES2312425T3 (en) |
| NO (1) | NO20025217L (en) |
| WO (1) | WO2001083733A1 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4204471B2 (en) * | 2001-10-22 | 2009-01-07 | 第一三共株式会社 | Screening method for cell wall acting drug |
| WO2003064422A1 (en) * | 2002-01-31 | 2003-08-07 | Daiichi Pharmaceutical Co., Ltd. | IMIDAZO[1,2-a]PYRIDINE DERIVATIVE |
| JP5296531B2 (en) | 2005-05-05 | 2013-09-25 | センシエント フレイバーズ エルエルシー | Production of β-glucan and mannan |
| CN111679069A (en) * | 2019-12-31 | 2020-09-18 | 安徽中医药大学 | Method for evaluating antifungal effect of traditional Chinese medicine monomer through auditory canal candida cell wall reconstruction |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6027910A (en) * | 1992-07-08 | 2000-02-22 | Unilever Patent Holdings B.V. | Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein |
| AU4566699A (en) * | 1998-06-25 | 2000-01-10 | Collaborative Group, Ltd., The | Beta(1,6)-glucan synthesis and cell wall assembly assay |
-
2001
- 2001-04-26 EP EP01925961A patent/EP1283261B1/en not_active Expired - Lifetime
- 2001-04-26 ES ES01925961T patent/ES2312425T3/en not_active Expired - Lifetime
- 2001-04-26 CA CA002407906A patent/CA2407906A1/en not_active Abandoned
- 2001-04-26 US US10/258,579 patent/US20040091949A1/en not_active Abandoned
- 2001-04-26 AU AU2001252604A patent/AU2001252604A1/en not_active Abandoned
- 2001-04-26 DE DE60135215T patent/DE60135215D1/en not_active Expired - Fee Related
- 2001-04-26 AT AT01925961T patent/ATE403717T1/en not_active IP Right Cessation
- 2001-04-26 WO PCT/JP2001/003630 patent/WO2001083733A1/en active IP Right Grant
-
2002
- 2002-10-31 NO NO20025217A patent/NO20025217L/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE403717T1 (en) | 2008-08-15 |
| EP1283261B1 (en) | 2008-08-06 |
| NO20025217L (en) | 2002-12-30 |
| EP1283261A1 (en) | 2003-02-12 |
| WO2001083733A1 (en) | 2001-11-08 |
| EP1283261A4 (en) | 2006-09-06 |
| CA2407906A1 (en) | 2001-11-08 |
| DE60135215D1 (en) | 2008-09-18 |
| NO20025217D0 (en) | 2002-10-31 |
| ES2312425T3 (en) | 2009-03-01 |
| US20040091949A1 (en) | 2004-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2003012058A3 (en) | Media and methods for cultivation of microorganisms | |
| MX9701112A (en) | Culture medium and device for detection and enumeration of enterobacteriaceae. | |
| AU2002323649A1 (en) | Rapid detection of replicating cells | |
| EP1347060A4 (en) | Method of detecting pathogenic microorganism | |
| AU2204795A (en) | Method for processing mycobacteria | |
| CA2126250A1 (en) | Method, reagents and kits for the detection of neisseria gonorrhoeae | |
| HK1094231A1 (en) | Prebiotic effect analysis | |
| AU2003261308A1 (en) | Method for preparing cell cultures from biological specimens for chemotherapeutic and other assays | |
| FR2785620B1 (en) | DETECTION OF ALL MICRO-ORGANISMS IN A SAMPLE | |
| AU1157301A (en) | Method of freeze-drying different microorganisms | |
| WO2003010304A3 (en) | Method for producing isolated cell cultures, culture medium for cultivating cell cultures and cell culture | |
| ATE519772T1 (en) | DRY CONTROL GENE SILENCE COMPOSITION APPARATUS AND SYSTEM | |
| WO2002036815A3 (en) | Genetic analysis of microorganisms | |
| AU2001252604A1 (en) | Method of screening drug acting on cell wall | |
| DK0839211T3 (en) | Process for the preparation of riboflavin by microorganisms with altered isocitrate lyase activity | |
| AU2159801A (en) | Test for micro-organisms | |
| ATE225859T1 (en) | DETECTION OF ANTIBIOTIC RESISTANCES IN MICROORGANISMS | |
| WO2004015141A3 (en) | Method for the rapid assessment of the presence and viability of bacterial cells and use thereof________________________ | |
| DK76189D0 (en) | PROCEDURE FOR PREPARING PROTEASE FROM ENDOTHIA PARASITICA | |
| WO2002101082A3 (en) | Culture medium for detecting and/or discriminating enterococcus and method therefor | |
| EP1445327A4 (en) | Method of screening drug acting on cell wall | |
| WO2003050289A8 (en) | Method for detecting microorganisms | |
| CA2137728A1 (en) | Enzymatic Removal of Non-Target Nucleic Acid from Biological Samples | |
| WO2003064679A3 (en) | Methods for detecting vancomycin-resistant microorganisms and compositions therefor | |
| WO2004078997A3 (en) | Method for detecting micro-organisms in an electrocoating process |