EA199600001A2 - Агент экстракции периплазматических рекомбинантных белков, способ экстракции периплазматического белка и способ культивирования прокариотического микроорганизма - Google Patents
Агент экстракции периплазматических рекомбинантных белков, способ экстракции периплазматического белка и способ культивирования прокариотического микроорганизмаInfo
- Publication number
- EA199600001A2 EA199600001A2 EA199600001A EA199600001A EA199600001A2 EA 199600001 A2 EA199600001 A2 EA 199600001A2 EA 199600001 A EA199600001 A EA 199600001A EA 199600001 A EA199600001 A EA 199600001A EA 199600001 A2 EA199600001 A2 EA 199600001A2
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- extraction
- arginine
- protein
- prokaryotic
- agrinin
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title abstract 6
- 239000004475 Arginine Substances 0.000 title abstract 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 title abstract 3
- 238000000605 extraction Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 108010090127 Periplasmic Proteins Proteins 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 210000001322 periplasm Anatomy 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5437—IL-13
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Изобретение относится к биотехнологии, а именно к культивированию прокариотического микроорганизма и экстракции рекомбинантных белков, продуцированных прокариотическими микроорганизмами. Сущность изобретения заключается в применении аргинина в качестве агента экстракции периплазматических рекомбинантных белков, а также в способе экстракции целевого периплазматического белка, в котором предусматривают суспендирование осадка клеток или клеточного дебриса клеток, полученных при культивировании прокариотического микроорганизма, трансформированного вектором экспрессии, содержащим ген, кодирующий указанный белок, и средства для его экспрессии в периплазму микроорганизма в буферном растворе, который содержит аргинин, и извлечение целевого белка из супернатанта полученной бактериальной суспензии. Способ также предусматривает культивирование прокариотического микроорганизма, трансформированного вектором экспрессии, содержащим ген, кодирующий целевой белок в присутствии агринина, при этом концентрация агринина составляет по крайней мере 2 г/л.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9501083A FR2729972B1 (fr) | 1995-01-31 | 1995-01-31 | Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| EA199600001A2 true EA199600001A2 (ru) | 1996-07-01 |
| EA199600001A3 EA199600001A3 (ru) | 1996-10-01 |
| EA000017B1 EA000017B1 (ru) | 1997-12-30 |
Family
ID=9475667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA199600001A EA000017B1 (ru) | 1995-01-31 | 1996-01-26 | Агент экстракции периплазматического рекомбинантного белка, способ получения периплазматического рекомбинантного белка и способ культивирования прокариотического микроорганизма |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US5700665A (ru) |
| EP (1) | EP0725140B1 (ru) |
| JP (1) | JP3005464B2 (ru) |
| KR (1) | KR100401028B1 (ru) |
| CN (1) | CN1132842C (ru) |
| AR (1) | AR000831A1 (ru) |
| AT (1) | ATE250132T1 (ru) |
| AU (1) | AU700509B2 (ru) |
| BR (1) | BR9600270A (ru) |
| CA (1) | CA2168382C (ru) |
| CZ (1) | CZ286509B6 (ru) |
| DE (1) | DE69629965D1 (ru) |
| EA (1) | EA000017B1 (ru) |
| FI (1) | FI960427A (ru) |
| FR (1) | FR2729972B1 (ru) |
| HU (1) | HU222598B1 (ru) |
| IL (1) | IL116973A0 (ru) |
| MX (1) | MX9600380A (ru) |
| NO (1) | NO316519B1 (ru) |
| NZ (1) | NZ280919A (ru) |
| PL (1) | PL183598B1 (ru) |
| SK (1) | SK281946B6 (ru) |
| TW (1) | TW403760B (ru) |
| UA (1) | UA27997C2 (ru) |
| ZA (1) | ZA96734B (ru) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1048732A1 (de) * | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
| KR20010056451A (ko) * | 1999-12-15 | 2001-07-04 | 윤재승 | 아르기닌이 강화된 동물세포 배양용 배지 조성물 |
| AU2003300295A1 (en) * | 2002-12-20 | 2004-07-22 | Cardiac Inventions Unlimited, Inc. | Left ventricular pacing lead and implantation method |
| BRPI0621124A2 (pt) * | 2005-12-22 | 2011-11-29 | Genentech Inc | métodos para recuperação de uma proteìna de ligação à heparina |
| WO2008008975A2 (en) | 2006-07-14 | 2008-01-17 | Genentech, Inc. | Refolding of recombinant proteins |
| GB201107737D0 (en) | 2011-05-09 | 2011-06-22 | Univ Birmingham | Extraction from cells |
| WO2022191223A1 (ja) * | 2021-03-10 | 2022-09-15 | 株式会社カネカ | 原核生物細胞から標的タンパク質を抽出する方法 |
| JPWO2022201917A1 (ru) | 2021-03-22 | 2022-09-29 | ||
| CN113249391A (zh) * | 2021-05-10 | 2021-08-13 | 江苏坤力生物制药有限责任公司 | 一种编码rPD蛋白的核酸、其制备方法和用途 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6244198A (ja) * | 1985-08-23 | 1987-02-26 | Toray Ind Inc | 有用タンパク質の生産方法 |
| JPS6251983A (ja) * | 1985-09-02 | 1987-03-06 | Hagiwara Yoshihide | ヒト／ヒト・ハイブリド−マ培養用無血清培地 |
| JPH0672105B2 (ja) * | 1985-10-02 | 1994-09-14 | 持田製薬株式会社 | 血栓溶解剤及びその製法 |
| DE3537708A1 (de) * | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| JP2769541B2 (ja) * | 1987-06-24 | 1998-06-25 | ジェネンテク，インコーポレイテッド | 平衡型構成誘導性転写系 |
| FR2636644B1 (fr) * | 1988-08-24 | 1990-12-28 | Sanofi Sa | Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide |
| PT100580A (pt) * | 1991-06-12 | 1993-09-30 | Regeneron Pharma | Producao e recuperacao de neurotrofinas recombinantes |
-
1995
- 1995-01-31 FR FR9501083A patent/FR2729972B1/fr not_active Expired - Fee Related
-
1996
- 1996-01-25 SK SK106-96A patent/SK281946B6/sk unknown
- 1996-01-26 EA EA199600001A patent/EA000017B1/ru not_active IP Right Cessation
- 1996-01-26 MX MX9600380A patent/MX9600380A/es unknown
- 1996-01-29 DE DE69629965T patent/DE69629965D1/de not_active Expired - Lifetime
- 1996-01-29 EP EP96400201A patent/EP0725140B1/fr not_active Expired - Lifetime
- 1996-01-29 UA UA96010341A patent/UA27997C2/uk unknown
- 1996-01-29 AT AT96400201T patent/ATE250132T1/de not_active IP Right Cessation
- 1996-01-30 NO NO960396A patent/NO316519B1/no not_active IP Right Cessation
- 1996-01-30 BR BR9600270A patent/BR9600270A/pt not_active Application Discontinuation
- 1996-01-30 HU HU9600209A patent/HU222598B1/hu not_active IP Right Cessation
- 1996-01-30 CA CA002168382A patent/CA2168382C/en not_active Expired - Fee Related
- 1996-01-30 PL PL96312543A patent/PL183598B1/pl not_active IP Right Cessation
- 1996-01-30 AR ARP960101196A patent/AR000831A1/es unknown
- 1996-01-30 KR KR1019960002120A patent/KR100401028B1/ko not_active IP Right Cessation
- 1996-01-30 FI FI960427A patent/FI960427A/fi not_active IP Right Cessation
- 1996-01-31 JP JP8015273A patent/JP3005464B2/ja not_active Expired - Fee Related
- 1996-01-31 CN CN96105578A patent/CN1132842C/zh not_active Expired - Fee Related
- 1996-01-31 AU AU42244/96A patent/AU700509B2/en not_active Ceased
- 1996-01-31 TW TW085101206A patent/TW403760B/zh not_active IP Right Cessation
- 1996-01-31 IL IL11697396A patent/IL116973A0/xx unknown
- 1996-01-31 NZ NZ280919A patent/NZ280919A/en unknown
- 1996-01-31 ZA ZA96734A patent/ZA96734B/xx unknown
- 1996-01-31 US US08/594,469 patent/US5700665A/en not_active Expired - Fee Related
- 1996-01-31 CZ CZ1996290A patent/CZ286509B6/cs not_active IP Right Cessation
-
1997
- 1997-08-06 US US08/906,957 patent/US5856142A/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AZ KZ KG TJ TM |
|
| PD4A | Registration of transfer of a eurasian patent in accordance with the succession in title | ||
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): BY RU |