KR960029343A - 아르기닌 존재시에 원핵 미생물로부터 주변세포질형 단백질의 추출방법 - Google Patents
아르기닌 존재시에 원핵 미생물로부터 주변세포질형 단백질의 추출방법 Download PDFInfo
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- KR960029343A KR960029343A KR1019960002120A KR19960002120A KR960029343A KR 960029343 A KR960029343 A KR 960029343A KR 1019960002120 A KR1019960002120 A KR 1019960002120A KR 19960002120 A KR19960002120 A KR 19960002120A KR 960029343 A KR960029343 A KR 960029343A
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- South Korea
- Prior art keywords
- arginine
- protein
- interest
- extracting
- peripheral
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract 21
- 102000004169 proteins and genes Human genes 0.000 title claims abstract 14
- 239000004475 Arginine Substances 0.000 title claims abstract 13
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 title claims abstract 13
- 230000001086 cytosolic effect Effects 0.000 title claims abstract 8
- 230000002093 peripheral effect Effects 0.000 title claims abstract 8
- 244000005700 microbiome Species 0.000 title claims abstract 7
- 238000000605 extraction Methods 0.000 title abstract 2
- 239000007853 buffer solution Substances 0.000 claims abstract 5
- 239000013604 expression vector Substances 0.000 claims abstract 4
- 239000008188 pellet Substances 0.000 claims abstract 4
- 230000001580 bacterial effect Effects 0.000 claims abstract 3
- 239000006228 supernatant Substances 0.000 claims abstract 3
- 239000000725 suspension Substances 0.000 claims abstract 3
- 238000012258 culturing Methods 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims 5
- 239000012670 alkaline solution Substances 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 210000000805 cytoplasm Anatomy 0.000 claims 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5437—IL-13
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 주변 세포질형 재조합 단백질의 추출제로서 아르기닌을 사용하는 관한 것으로, 특히 관심있는 주변세포질형 단백질의 추출이: (ⅰ) 아르기닌을 포함하는 버퍼 용액 내에서, 원핵 미생물의 주변 세포질에서의 벡터 발현에 필요한 요소 및 상기 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양을 통해 생겨나는 세포로부터의 세포데브리 펠럿이나 세포 펠럿을 현탁시키는 단계; 및 (ⅱ) 상기 단계를 통해 수득된 박테리아 현탁액의 상청액에서 관심있는 단백질을 회수하는 단계로 이루어진 주변 세포질형 단백질의 추출방법에 관한 것이다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (7)
- 주변세포질형 제조합 단백질의 추출제용으로 사용되는 아르기닌.
- 관심있는 주변 세포질형 단백질의 추출방법으로서: -아르기닌을 포함하는 버퍼 용액내에서, 원핵 미생물의 주변세포질에서의 발현에 필요한 요소 및 상기 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양물로부터 생겨나는 페포펠럿을 현탁시키는 단계; 및 - 상기 단계를 통해 수득된 박테리아 현탁액의 상청액에서 관심있는 단백질을 회수하는 단계로 이루어지는 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
- 관심있는 주변세포질형 단백질의 추출방법으로서: -아르기닌을 포함하는 버퍼 용액내에서, 원핵 미생물의 주변 세포질에서의 발현에 필요한 요소 및 상기 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양물로부터 생겨나는 세포로 부터의 세포데브리 펠럿을 현탁시키는 단계; 및 - 상기 단계를 통해 수득된 박테리아 현탁액의 상청액에서 관심있는 단백질을 회수하는 단계로 이루어지는 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
- 제2항에 있어서, 아르기닌을 포함하는 버퍼용액이, 0.4M 이상과 동등한 아르기닌 농도를 갖는 알칼리 수용액인 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
- 제3항에 있어서, 아르기닌을 포함하는 버퍼용액이, 0.4M 이상과 동등한 아르기닌 농도를 갖는 알칼리 수용액인 것을 특징으로 하는 주변 세포질형 단백질의 추출방법.
- 관심있는 단백질을 코오딩하는 유전자를 포함하는 발현벡터에 의해 형질전환된 원핵 미생물의 배양에 있어서, 2g/1 이상과 동등한 농도의 아르기닌 존재하에서 배양이 수행되는 것을 특징으로 하는 원핵 미생물의 배양방법.
- 제6항에 있어서, 2g/1와 10g/1 사이 농도의 아르기닌 존재하에서 배양이 수행되는 것을 특징으로 하는 원핵 미생물의 배양방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9501083 | 1995-01-31 | ||
| FR95-01083 | 1995-01-31 | ||
| FR9501083A FR2729972B1 (fr) | 1995-01-31 | 1995-01-31 | Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR960029343A true KR960029343A (ko) | 1996-08-17 |
| KR100401028B1 KR100401028B1 (ko) | 2004-03-31 |
Family
ID=9475667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019960002120A KR100401028B1 (ko) | 1995-01-31 | 1996-01-30 | 아르기닌존재하의원핵미생물로부터주변세포질형단백질의추출방법 |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US5700665A (ko) |
| EP (1) | EP0725140B1 (ko) |
| JP (1) | JP3005464B2 (ko) |
| KR (1) | KR100401028B1 (ko) |
| CN (1) | CN1132842C (ko) |
| AR (1) | AR000831A1 (ko) |
| AT (1) | ATE250132T1 (ko) |
| AU (1) | AU700509B2 (ko) |
| BR (1) | BR9600270A (ko) |
| CA (1) | CA2168382C (ko) |
| CZ (1) | CZ286509B6 (ko) |
| DE (1) | DE69629965D1 (ko) |
| EA (1) | EA000017B1 (ko) |
| FI (1) | FI960427A (ko) |
| FR (1) | FR2729972B1 (ko) |
| HU (1) | HU222598B1 (ko) |
| IL (1) | IL116973A0 (ko) |
| MX (1) | MX9600380A (ko) |
| NO (1) | NO316519B1 (ko) |
| NZ (1) | NZ280919A (ko) |
| PL (1) | PL183598B1 (ko) |
| SK (1) | SK281946B6 (ko) |
| TW (1) | TW403760B (ko) |
| UA (1) | UA27997C2 (ko) |
| ZA (1) | ZA96734B (ko) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1048732A1 (de) * | 1999-04-26 | 2000-11-02 | F. Hoffmann-La Roche Ag | Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen |
| KR20010056451A (ko) * | 1999-12-15 | 2001-07-04 | 윤재승 | 아르기닌이 강화된 동물세포 배양용 배지 조성물 |
| AU2003300295A1 (en) * | 2002-12-20 | 2004-07-22 | Cardiac Inventions Unlimited, Inc. | Left ventricular pacing lead and implantation method |
| BRPI0621124A2 (pt) * | 2005-12-22 | 2011-11-29 | Genentech Inc | métodos para recuperação de uma proteìna de ligação à heparina |
| WO2008008975A2 (en) | 2006-07-14 | 2008-01-17 | Genentech, Inc. | Refolding of recombinant proteins |
| GB201107737D0 (en) | 2011-05-09 | 2011-06-22 | Univ Birmingham | Extraction from cells |
| WO2022191223A1 (ja) * | 2021-03-10 | 2022-09-15 | 株式会社カネカ | 原核生物細胞から標的タンパク質を抽出する方法 |
| JPWO2022201917A1 (ko) | 2021-03-22 | 2022-09-29 | ||
| CN113249391A (zh) * | 2021-05-10 | 2021-08-13 | 江苏坤力生物制药有限责任公司 | 一种编码rPD蛋白的核酸、其制备方法和用途 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6244198A (ja) * | 1985-08-23 | 1987-02-26 | Toray Ind Inc | 有用タンパク質の生産方法 |
| JPS6251983A (ja) * | 1985-09-02 | 1987-03-06 | Hagiwara Yoshihide | ヒト／ヒト・ハイブリド−マ培養用無血清培地 |
| JPH0672105B2 (ja) * | 1985-10-02 | 1994-09-14 | 持田製薬株式会社 | 血栓溶解剤及びその製法 |
| DE3537708A1 (de) * | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| JP2769541B2 (ja) * | 1987-06-24 | 1998-06-25 | ジェネンテク，インコーポレイテッド | 平衡型構成誘導性転写系 |
| FR2636644B1 (fr) * | 1988-08-24 | 1990-12-28 | Sanofi Sa | Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide |
| PT100580A (pt) * | 1991-06-12 | 1993-09-30 | Regeneron Pharma | Producao e recuperacao de neurotrofinas recombinantes |
-
1995
- 1995-01-31 FR FR9501083A patent/FR2729972B1/fr not_active Expired - Fee Related
-
1996
- 1996-01-25 SK SK106-96A patent/SK281946B6/sk unknown
- 1996-01-26 EA EA199600001A patent/EA000017B1/ru not_active IP Right Cessation
- 1996-01-26 MX MX9600380A patent/MX9600380A/es unknown
- 1996-01-29 DE DE69629965T patent/DE69629965D1/de not_active Expired - Lifetime
- 1996-01-29 EP EP96400201A patent/EP0725140B1/fr not_active Expired - Lifetime
- 1996-01-29 UA UA96010341A patent/UA27997C2/uk unknown
- 1996-01-29 AT AT96400201T patent/ATE250132T1/de not_active IP Right Cessation
- 1996-01-30 NO NO960396A patent/NO316519B1/no not_active IP Right Cessation
- 1996-01-30 BR BR9600270A patent/BR9600270A/pt not_active Application Discontinuation
- 1996-01-30 HU HU9600209A patent/HU222598B1/hu not_active IP Right Cessation
- 1996-01-30 CA CA002168382A patent/CA2168382C/en not_active Expired - Fee Related
- 1996-01-30 PL PL96312543A patent/PL183598B1/pl not_active IP Right Cessation
- 1996-01-30 AR ARP960101196A patent/AR000831A1/es unknown
- 1996-01-30 KR KR1019960002120A patent/KR100401028B1/ko not_active IP Right Cessation
- 1996-01-30 FI FI960427A patent/FI960427A/fi not_active IP Right Cessation
- 1996-01-31 JP JP8015273A patent/JP3005464B2/ja not_active Expired - Fee Related
- 1996-01-31 CN CN96105578A patent/CN1132842C/zh not_active Expired - Fee Related
- 1996-01-31 AU AU42244/96A patent/AU700509B2/en not_active Ceased
- 1996-01-31 TW TW085101206A patent/TW403760B/zh not_active IP Right Cessation
- 1996-01-31 IL IL11697396A patent/IL116973A0/xx unknown
- 1996-01-31 NZ NZ280919A patent/NZ280919A/en unknown
- 1996-01-31 ZA ZA96734A patent/ZA96734B/xx unknown
- 1996-01-31 US US08/594,469 patent/US5700665A/en not_active Expired - Fee Related
- 1996-01-31 CZ CZ1996290A patent/CZ286509B6/cs not_active IP Right Cessation
-
1997
- 1997-08-06 US US08/906,957 patent/US5856142A/en not_active Expired - Fee Related
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