RU94042389A - Промотор, вектор, система экспрессии - Google Patents

Промотор, вектор, система экспрессии

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Publication number
RU94042389A
RU94042389A RU94042389/13A RU94042389A RU94042389A RU 94042389 A RU94042389 A RU 94042389A RU 94042389/13 A RU94042389/13 A RU 94042389/13A RU 94042389 A RU94042389 A RU 94042389A RU 94042389 A RU94042389 A RU 94042389A
Authority
RU
Russia
Prior art keywords
promoters
native
promoter
level
inducible
Prior art date
Application number
RU94042389/13A
Other languages
English (en)
Other versions
RU2128225C1 (ru
Inventor
Филмас Джордж
Ca]
Клейн Мишель
Original Assignee
Коннот Лабораториз Лимитед (CA)
Коннот Лабораториз Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Коннот Лабораториз Лимитед (CA), Коннот Лабораториз Лимитед filed Critical Коннот Лабораториз Лимитед (CA)
Publication of RU94042389A publication Critical patent/RU94042389A/ru
Application granted granted Critical
Publication of RU2128225C1 publication Critical patent/RU2128225C1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Orthopedics, Nursing, And Contraception (AREA)
  • Electrophonic Musical Instruments (AREA)
  • Soil Working Implements (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Measuring And Recording Apparatus For Diagnosis (AREA)
  • Electrotherapy Devices (AREA)
  • Developing Agents For Electrophotography (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Multicomponent Fibers (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Синтетические индуцибельные эукариотические промоторы, регулирующие транскрипцию гена, приводят к повышению уровня экспрессии белка и снижению основного уровня экспрессии гена. Такие промоторы содержат как минимум два различных класса индуцибельных элементов, получаемых на основе нативного промотора, при этом один из них является нативным индуцибельным элементом, а второй получается за счет инсерции в указанный нативный промотор. В приведенных методиках к нативным промоторам, в частности hMT-IIA и MMTV - LTR, добавляются дополнительные элементы, отвечающие на индукцию металлом (МОЭ, MRE) и/или элементы, отвечающие на индукцию глюкокортикоидом (ГОЭ, GREs). Один или более конститутивных элемента могут иметь функциональные нарушения с целью понижения уровня основной экспрессии гена.

Claims (1)

  1. Синтетические индуцибельные эукариотические промоторы, регулирующие транскрипцию гена, приводят к повышению уровня экспрессии белка и снижению основного уровня экспрессии гена. Такие промоторы содержат как минимум два различных класса индуцибельных элементов, получаемых на основе нативного промотора, при этом один из них является нативным индуцибельным элементом, а второй получается за счет инсерции в указанный нативный промотор. В приведенных методиках к нативным промоторам, в частности hMT-IIA и MMTV - LTR, добавляются дополнительные элементы, отвечающие на индукцию металлом (МОЭ, MRE) и/или элементы, отвечающие на индукцию глюкокортикоидом (ГОЭ, GREs). Один или более конститутивных элемента могут иметь функциональные нарушения с целью понижения уровня основной экспрессии гена.
RU94042389A 1992-03-30 1993-03-30 Синтетический индуцибельный эукариотический промотор для регуляции транскрипции гена (варианты) RU2128225C1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB929206874A GB9206874D0 (en) 1992-03-30 1992-03-30 Generation of improved inducible mammalian expression vectors
GB9206874.1 1992-03-30
PCT/CA1993/000130 WO1993020218A1 (en) 1992-03-30 1993-03-30 Synthetic eukaryotic promoters containing two inducible elements

Publications (2)

Publication Number Publication Date
RU94042389A true RU94042389A (ru) 1997-03-10
RU2128225C1 RU2128225C1 (ru) 1999-03-27

Family

ID=10713085

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94042389A RU2128225C1 (ru) 1992-03-30 1993-03-30 Синтетический индуцибельный эукариотический промотор для регуляции транскрипции гена (варианты)

Country Status (17)

Country Link
US (1) US5559027A (ru)
EP (1) EP0633941B1 (ru)
JP (1) JP2701983B2 (ru)
KR (1) KR100208311B1 (ru)
AT (1) ATE217649T1 (ru)
AU (1) AU667532B2 (ru)
BR (1) BR9306167A (ru)
CA (1) CA2128602C (ru)
DE (1) DE69331931T2 (ru)
DK (1) DK0633941T3 (ru)
ES (1) ES2176202T3 (ru)
FI (1) FI113548B (ru)
GB (1) GB9206874D0 (ru)
NO (1) NO316451B1 (ru)
PT (1) PT633941E (ru)
RU (1) RU2128225C1 (ru)
WO (1) WO1993020218A1 (ru)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6071695A (en) * 1992-02-21 2000-06-06 Creative Biomolecules, Inc. Methods and products for identification of modulators of osteogenic protein-1 gene expression
US5512483A (en) * 1993-05-21 1996-04-30 Mcgill University Expression vectors responsive to steroid hormones
JPH07163368A (ja) * 1993-12-15 1995-06-27 Hayashibara Biochem Lab Inc 組換えｄｎａとその組換えｄｎａを含む形質転換体
AU703445B2 (en) * 1994-06-07 1999-03-25 Stryker Corporation Methods and compositions for modulating morphogenic protein expression
JP2001509395A (ja) 1997-07-14 2001-07-24 バレンティス・インコーポレーテッド 細胞又は組織特異的な人工転写調節領域の同定方法
JP2001520170A (ja) 1997-10-16 2001-10-30 ボード・オヴ・リージェンツ，ザ・ユニヴァーシティ・オヴ・テキサス・システム Ｎｆ−ａｔ３機能に関連した心肥大動物モデルと治療法
FR2772786A1 (fr) * 1997-12-18 1999-06-25 Inst Nat Sante Rech Med Sequences nucleotidiques chevauchantes, et leur utilisation dans le cadre de la detection de xenohormones ainsi que dans des compositions pharmaceutiques
CA2362970A1 (en) * 1999-02-19 2000-08-24 Octagene Gmbh Hormone-hormone receptor complexes and nucleic acid constructs and their use in gene therapy
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
US7341847B2 (en) * 2003-04-02 2008-03-11 Agency For Science, Technology And Research Promoter construct for gene expression in neuronal cells
WO2005046450A2 (en) 2003-11-12 2005-05-26 Children's Hospital Medical Center Method for diagnosis and treatment of pulmonary disorders
KR100647490B1 (ko) * 2004-10-04 2006-11-23 사회복지법인 삼성생명공익재단 저산소 조건 및 다른 스트레스에 특이적으로 반응할 수있는 키메라 인핸서 요소를 포함하는 벡터, 상기 벡터를포함하는 약제학적 조성물 및 상기 조성물을 이용하는 방법
JP2009508596A (ja) 2005-09-19 2009-03-05 ヒストジェニックス コーポレイション 細胞支持基材及びその調製方法
CN101583651A (zh) * 2006-09-04 2009-11-18 生物能源株式会社 聚酯多元醇
RU2370280C2 (ru) * 2007-10-08 2009-10-20 Институт физико-химической биологии им. А.Н.Белозерского СПОСОБ СУПЕРПРОДУКЦИИ В РАСТЕНИИ АНТИТЕЛ ПРОТИВ ОНКОГЕНА HER2/neu
DK2393927T3 (en) 2009-02-09 2015-03-30 Morphosys Ag Preparation of oligoclonal mixtures of antibodies in the individual cells
WO2011146833A1 (en) 2010-05-20 2011-11-24 Evolva Inc. Method of producing isoprenoid compounds in yeast
US10077420B2 (en) 2014-12-02 2018-09-18 Histogenics Corporation Cell and tissue culture container
WO2019106035A1 (en) 2017-11-30 2019-06-06 Friedrich Miescher Institute For Biomedical Research Synpiii, a promoter for the specific expression of genes in retinal pigment epithelium

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58502180A (ja) * 1981-11-23 1983-12-22 ユニヴアシテイ　パテンツ，インコ−ポレイテツド Ｄｎａ配列の転写の調節
EP0511285A4 (en) * 1990-01-18 1993-05-26 Us Commerce Vector with multiple target response elements affecting gene expression
CA2075182C (en) * 1990-02-09 2003-03-25 David J. Mangelsdorf Retinoid receptor compositions and methods

Also Published As

Publication number Publication date
DE69331931D1 (de) 2002-06-20
KR100208311B1 (ko) 1999-07-15
DK0633941T3 (da) 2002-06-17
KR950700420A (ko) 1995-01-16
NO943610L (no) 1994-11-30
PT633941E (pt) 2002-10-31
CA2128602A1 (en) 1993-10-14
CA2128602C (en) 2008-01-22
FI944451A0 (fi) 1994-09-26
WO1993020218A1 (en) 1993-10-14
DE69331931T2 (de) 2002-09-12
AU3883393A (en) 1993-11-08
RU2128225C1 (ru) 1999-03-27
NO316451B1 (no) 2004-01-26
GB9206874D0 (en) 1992-05-13
ATE217649T1 (de) 2002-06-15
EP0633941A1 (en) 1995-01-18
NO943610D0 (no) 1994-09-29
US5559027A (en) 1996-09-24
JP2701983B2 (ja) 1998-01-21
FI944451A (fi) 1994-09-26
BR9306167A (pt) 1998-01-13
FI113548B (fi) 2004-05-14
EP0633941B1 (en) 2002-05-15
AU667532B2 (en) 1996-03-28
ES2176202T3 (es) 2002-12-01
JPH07501456A (ja) 1995-02-16

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