ES2058108T3 - Sistema de expresion de genes. - Google Patents

Sistema de expresion de genes.

Info

Publication number
ES2058108T3
ES2058108T3 ES87303397T ES87303397T ES2058108T3 ES 2058108 T3 ES2058108 T3 ES 2058108T3 ES 87303397 T ES87303397 T ES 87303397T ES 87303397 T ES87303397 T ES 87303397T ES 2058108 T3 ES2058108 T3 ES 2058108T3
Authority
ES
Spain
Prior art keywords
acting
trans
gene expression
dna segment
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87303397T
Other languages
English (en)
Inventor
Sven Hastrup
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2058108T3 publication Critical patent/ES2058108T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

SISTEMAS DE EXPRESION GENETICA QUE COMPRENDEN UN VECTOR DE EXPRESION Y UN SEGMENTO DE ADN "DE ACTUACION TRANS" DONDE EL VECTOR DE EXPRESION COMPRENDE EL GEN O GENES QUE SERAN EXPRESADOS Y UNO O MAS ELEMENTOS REGULADORES "DE ACTUACION CIS" QUE SON CORRESPONDIENTES A UN FACTOR "DE ACTUACION TRANS" PRODUCIDO POR DICHO SEGMENTO DE ADN "DE ACTUACION TRANS". MAS ESPECIALMENTE, LA INVENCION SE REFIERE A DICHOS SISTEMAS DE EXPRESION GENETICA DONDE DICHO SEGMENTO DE ADN "DE ACTUACION TRANS" Y DICHOS ELEMENTOS REGULADORES "DE ACTUACION CIS" COMPRENDEN UNO O MAS SEGMENTOS DEL GENOMA DE UNA ESPECIE DE BACILLUS. TAMBIEN SE DECUBREN METODOS PARA ESTIMULAR LA PRODUCCION DE PRODUCTOS GENETICOS, VECTORES PARA TRANSFORMAR MICROORGANISMOS, Y SU USO.
ES87303397T 1986-04-17 1987-04-16 Sistema de expresion de genes. Expired - Lifetime ES2058108T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK177786A DK160947C (da) 1986-04-17 1986-04-17 Gen-udtrykkelsessystem

Publications (1)

Publication Number Publication Date
ES2058108T3 true ES2058108T3 (es) 1994-11-01

Family

ID=8107985

Family Applications (1)

Application Number Title Priority Date Filing Date
ES87303397T Expired - Lifetime ES2058108T3 (es) 1986-04-17 1987-04-16 Sistema de expresion de genes.

Country Status (7)

Country Link
US (1) US5036002A (es)
EP (1) EP0242220B1 (es)
JP (1) JPS63157987A (es)
AT (1) ATE91715T1 (es)
DE (1) DE3786586T2 (es)
DK (1) DK160947C (es)
ES (1) ES2058108T3 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8807939D0 (en) * 1988-04-05 1988-05-05 Univ Brunel Regulatory cassette for expression vectors
EP0347915A3 (en) * 1988-06-23 1991-03-20 The Rockefeller University Method and agents for inhibiting low density lipoprotein synthesis
PL184463B1 (pl) * 1995-06-23 2002-11-29 Danisco Ingredients As Kaseta kwasu nukleinowego wektor komórka gospodarza i ich zastosowania fragment kwasu nukleinowego mutant sekwencji aminokwasowej sposób wytwarzania i selekcjonowania mutantów oraz sposób identyfikacji i oznaczania sekwencji kwasu nukleinowego
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
WO2000071691A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 125 and 126
EP1183342B2 (en) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
EP1183341B2 (en) 1999-05-20 2012-05-02 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
US6238273B1 (en) 1999-08-31 2001-05-29 Micron Technology, Inc. Methods for predicting polishing parameters of polishing pads and methods and machines for planarizing microelectronic substrate assemblies in mechanical or chemical-mechanical planarization
GB0116798D0 (en) * 2001-07-10 2001-08-29 Secr Defence Expression system
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
WO2005043132A1 (en) * 2003-10-31 2005-05-12 Applied Materials, Inc. Polishing endpoint detection system and method using friction sensor

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3484078D1 (de) * 1983-06-27 1991-03-14 Genentech Inc Uebertragbare induzierbare kontrollsysteme, diese enthaltende expressionsvektoren, mit diesen transformierte mikroorganismen und ihre verwendung bei der expression von exogenem protein.
JPS6075286A (ja) * 1983-09-28 1985-04-27 Hirosuke Okada キシラナ−ゼ遺伝子を有する組み換え体プラスミドおよびそれにより形質転換されたＢａｃｉｌｌｕｓ属菌
GB2166743B (en) * 1984-11-13 1989-01-05 Solvay Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression
JPH09198978A (ja) * 1996-01-23 1997-07-31 Advantest Corp 光導電スイッチ

Also Published As

Publication number Publication date
DK160947B (da) 1991-05-06
US5036002A (en) 1991-07-30
DK177786D0 (da) 1986-04-17
JPS63157987A (ja) 1988-06-30
DK160947C (da) 1991-10-21
DE3786586D1 (de) 1993-08-26
EP0242220A2 (en) 1987-10-21
EP0242220B1 (en) 1993-07-21
EP0242220A3 (en) 1989-05-03
DE3786586T2 (de) 1993-10-28
DK177786A (da) 1988-02-16
ATE91715T1 (de) 1993-08-15

Similar Documents

Publication Publication Date Title
ES2058108T3 (es) Sistema de expresion de genes.
AU5437886A (en) Phenotypic modifications of host cells via RNA transformation vector
GB2136814B (en) Dna vectors
ATE251218T1 (de) Herstellung von polyalkanoat
IL86724A0 (en) Transcription-based nucleic acid amplification/detection systems
DE3772485D1 (de) Rekombinante dns-sequenzen, diese enthaltende vektoren und verfahren zur verwendung derselben.
YU65194A (sh) Promotorni elementi himernih gena za alfa-tubulin
ES2056799T3 (es) Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
DK0519053T3 (da) Nucleinsyreamplifikation med DNA-afhængig RNA-polymeraseaktivitet hos RNA-replicaser
DK28191D0 (da) Dna-planteekspressionskassette og dennes anvendelse
ATE315087T1 (de) Neuartige auf p53 ansprechende gene
JPS6437294A (en) Protection of plant from virus infection or sequela
DE69734512D1 (de) Steigerung der genexpression
ATE237693T1 (de) Rekombinante foamy-virus-vektoren zur medizinischen und diagnostischen verwendung und verfahren zur herstellung rekombinanter foamy- virus-vektoren
DE3782844D1 (de) Vektoren fuer die klonierung und expression heterologer gene in hefen und durch diese vektoren transformierte hefestaemme.
BR9704067A (pt) Produção melhorada de riboflavina
HUP9802681A1 (hu) Onkogénnel vagy vírussal szabályozott expressziós rendszerek
NZ307444A (en) Polyphenol oxidase genes from lettuce and banana
AU6144294A (en) Plant virus resistance conferring polyribozyme and resistant plants producing same
ATE72837T1 (de) Vielfach amplifizierbare vektoren fuer hohe expression exogener dns.
ATE240400T1 (de) Verfahren zur erzeugung von pathogen-resistenten pflanzen
EP0128042A3 (en) A process for indentifying or isolating a dna sequence, and a dna hybridization probe therefor
IT8521507A0 (it) Vettore di clonaggio, molecole di dna ricombinante, ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni.
TH7509EX (th) กระบวนการที่ใช้ในการรวมยีนที่เลือกสรรแล้วเข้าเป็นหน่วยเดียวกันกับโครโมโซมของแบคทีเรีย และแบคทีเรียที่ได้จากกระบวนการดังกล่าว
MX9709380A (es) Acidos nucleicos virales recombinantes.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 242220

Country of ref document: ES