ATE250132T1 - Verfahren zur extraktion von periplasmatischen proteinen aus prokaryotischen mikroorganismen in der anwesenheit von arginin - Google Patents

Verfahren zur extraktion von periplasmatischen proteinen aus prokaryotischen mikroorganismen in der anwesenheit von arginin

Info

Publication number
ATE250132T1
ATE250132T1 AT96400201T AT96400201T ATE250132T1 AT E250132 T1 ATE250132 T1 AT E250132T1 AT 96400201 T AT96400201 T AT 96400201T AT 96400201 T AT96400201 T AT 96400201T AT E250132 T1 ATE250132 T1 AT E250132T1
Authority
AT
Austria
Prior art keywords
arginine
extracting
prokaryotic microorganisms
periplasmatic
proteins
Prior art date
Application number
AT96400201T
Other languages
English (en)
Inventor
Richard Legoux
Paul Maldonado
Marc Salome
Original Assignee
Sanofi Synthelabo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Synthelabo filed Critical Sanofi Synthelabo
Application granted granted Critical
Publication of ATE250132T1 publication Critical patent/ATE250132T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormones [GH] (Somatotropin)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/54Interleukins [IL]
    • C07K14/5437IL-13
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT96400201T 1995-01-31 1996-01-29 Verfahren zur extraktion von periplasmatischen proteinen aus prokaryotischen mikroorganismen in der anwesenheit von arginin ATE250132T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9501083A FR2729972B1 (fr) 1995-01-31 1995-01-31 Procede d'extraction de proteines periplasmiques de microorganismes procaryotes en presence d'arginine

Publications (1)

Publication Number Publication Date
ATE250132T1 true ATE250132T1 (de) 2003-10-15

Family

ID=9475667

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96400201T ATE250132T1 (de) 1995-01-31 1996-01-29 Verfahren zur extraktion von periplasmatischen proteinen aus prokaryotischen mikroorganismen in der anwesenheit von arginin

Country Status (25)

Country Link
US (2) US5700665A (de)
EP (1) EP0725140B1 (de)
JP (1) JP3005464B2 (de)
KR (1) KR100401028B1 (de)
CN (1) CN1132842C (de)
AR (1) AR000831A1 (de)
AT (1) ATE250132T1 (de)
AU (1) AU700509B2 (de)
BR (1) BR9600270A (de)
CA (1) CA2168382C (de)
CZ (1) CZ286509B6 (de)
DE (1) DE69629965D1 (de)
EA (1) EA000017B1 (de)
FI (1) FI960427A (de)
FR (1) FR2729972B1 (de)
HU (1) HU222598B1 (de)
IL (1) IL116973A0 (de)
MX (1) MX9600380A (de)
NO (1) NO316519B1 (de)
NZ (1) NZ280919A (de)
PL (1) PL183598B1 (de)
SK (1) SK281946B6 (de)
TW (1) TW403760B (de)
UA (1) UA27997C2 (de)
ZA (1) ZA96734B (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
KR20010056451A (ko) * 1999-12-15 2001-07-04 윤재승 아르기닌이 강화된 동물세포 배양용 배지 조성물
US20040215139A1 (en) * 2002-12-20 2004-10-28 Todd Cohen Apparatus and method for implanting left ventricular pacing leads within the coronary sinus
CA2633554C (en) * 2005-12-22 2012-11-13 Genetech, Inc. Recombinant production of heparin binding proteins
CN101506222B (zh) 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
GB201107737D0 (en) 2011-05-09 2011-06-22 Univ Birmingham Extraction from cells
WO2022191223A1 (ja) * 2021-03-10 2022-09-15 株式会社カネカ 原核生物細胞から標的タンパク質を抽出する方法
EP4317169A1 (de) 2021-03-22 2024-02-07 Kaneka Corporation Verfahren zur herstellung von fremdproteinen mit escherichia coli
CN113249391A (zh) * 2021-05-10 2021-08-13 江苏坤力生物制药有限责任公司 一种编码rPD蛋白的核酸、其制备方法和用途

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6244198A (ja) * 1985-08-23 1987-02-26 Toray Ind Inc 有用タンパク質の生産方法
JPS6251983A (ja) * 1985-09-02 1987-03-06 Hagiwara Yoshihide ヒト/ヒト・ハイブリド−マ培養用無血清培地
JPH0672105B2 (ja) * 1985-10-02 1994-09-14 持田製薬株式会社 血栓溶解剤及びその製法
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
WO1988010307A1 (en) * 1987-06-24 1988-12-29 Genentech, Inc. Balanced constitutive inducible transcription system
FR2636644B1 (fr) * 1988-08-24 1990-12-28 Sanofi Sa Sequence d'adn participant a la regulation de l'expression d'une sequence d'adn codant pour un precurseur d'un polypeptide, vecteurs d'expression et procede de production periplasmique du polypeptide
NZ243084A (en) * 1991-06-12 1995-08-28 Regeneron Pharma Production and recovery of recombinant neurotrophins, genes for a modified lamb signal sequence, fusion genes and plasmids

Also Published As

Publication number Publication date
KR100401028B1 (ko) 2004-03-31
IL116973A0 (en) 1996-05-14
EP0725140B1 (de) 2003-09-17
PL183598B1 (pl) 2002-06-28
CN1132842C (zh) 2003-12-31
HU222598B1 (hu) 2003-08-28
BR9600270A (pt) 1997-12-23
PL312543A1 (en) 1996-08-05
CZ29096A3 (en) 1996-08-14
UA27997C2 (uk) 2000-10-16
EA000017B1 (ru) 1997-12-30
CA2168382A1 (en) 1996-08-01
FI960427A (fi) 1996-08-01
ZA96734B (en) 1996-08-16
NZ280919A (en) 1997-07-27
HUP9600209A2 (en) 1997-05-28
AR000831A1 (es) 1997-08-06
SK281946B6 (sk) 2001-09-11
MX9600380A (es) 1997-01-31
HUP9600209A3 (en) 2000-08-28
CA2168382C (en) 2003-09-30
FI960427A0 (fi) 1996-01-30
AU4224496A (en) 1996-08-08
JPH08242879A (ja) 1996-09-24
NO316519B1 (no) 2004-02-02
NO960396L (no) 1996-08-01
TW403760B (en) 2000-09-01
HU9600209D0 (en) 1996-03-28
US5700665A (en) 1997-12-23
EA199600001A2 (ru) 1996-07-01
KR960029343A (ko) 1996-08-17
US5856142A (en) 1999-01-05
DE69629965D1 (de) 2003-10-23
EP0725140A1 (de) 1996-08-07
AU700509B2 (en) 1999-01-07
JP3005464B2 (ja) 2000-01-31
FR2729972B1 (fr) 1997-04-18
CN1142502A (zh) 1997-02-12
SK10696A3 (en) 1996-09-04
NO960396D0 (no) 1996-01-30
FR2729972A1 (fr) 1996-08-02
EA199600001A3 (ru) 1996-10-01
CZ286509B6 (en) 2000-04-12

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