KR860002571A - 재조합 폴리펩티드의 분리 방법 - Google Patents

재조합 폴리펩티드의 분리 방법 Download PDF

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KR860002571A
KR860002571A KR1019850006323A KR850006323A KR860002571A KR 860002571 A KR860002571 A KR 860002571A KR 1019850006323 A KR1019850006323 A KR 1019850006323A KR 850006323 A KR850006323 A KR 850006323A KR 860002571 A KR860002571 A KR 860002571A
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bacterial cells
acid
produced
polypeptide
recombinant methods
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KR1019850006323A
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KR930002886B1 (ko
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아돈프(외 3) 군테르
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디터라우디엔, 프리츠 좀머
베링거 인겔하임 인터내셔널 지엠비에이취
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Molecular Biology (AREA)
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  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

재조합 폴리펩티드의 분리 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 세균 세포를 분해하는 디스팩스-리액터(Dispax-Reactor) 3-6/6형 균질화기의 횡단면도.

Claims (10)

  1. 유전 공학적으로 생성된 폴리펩파드를 함유하는 세균 세포를 pH 내지 2.0의 수성산에 현탁시키고 기계적으로 파괴시킨 다음에, 생성된 현탁액중에 존재하는 폴리펩티드를 분리 및 정제함을 특징으로 하여, 재조합 방법에 의해 생성된 폴리펩티드를 세균 세포로 부터 분리하는 방법.
  2. 제1항에 있어서, 세균 세포로 재조합 방법에 의해 생성된 산-안정성 인터페론을 함유하는 세균 세포를 사용하는 방법.
  3. 제1항에 있어서, 세균 세포로 재조합 방법에 의해 생성된 알파-인터페론을 함유하는 세균 세포를 사용하는 방법.
  4. 제1항에 있어서, 세균 세포로 재조합방법에 의해 생성된 IFN-α2(arg)를 함유하는 세균세포를 사용하는 방법.
  5. 제2항 내지 제4항중 어느 하나에 있어서, pH 2.5 내지 3.5에서 수행하는 방법.
  6. 제1항 내지 제5항중 어느 하나에 있어서, 생성된 전단력에 의해 세균 세포를 파괴시키는 방법.
  7. 제1항 내지 제6항중 어느 하나에 있어서, 세균 세포를 균질화기를 사용하여 파괴시키는 방법.
  8. 제7항에 있어서, 사용되는 균질화기가 울트라-투랙스형(ultra-Turrax type), 바람직하게는 디스팩스-리액터 3-6/6(Dispax-Reactor 3-6/6)의 균질화기인 방법.
  9. 제1항 내지 제8항중 어느 하나에 있어서, 사용되는 산이무기산, 또는 아세트산이나 프로피온산과 같은 알카노산인 방법.
  10. 제1항 내지 제9항중 어느 하나에 있어서, 사용되는 수성산이 pH 2.5의 묽은 염산, 또는 1% 아세트산인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019850006323A 1984-09-01 1985-08-30 재조합 폴리펩티드의 분리방법 KR930002886B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19843432196 DE3432196A1 (de) 1984-09-01 1984-09-01 Neues mechanisches aufschlussverfahren von bakterienzellen zur isolierung von rekombinant hergestellten peptiden
DEP3432196.9 1984-09-01
ATP3432196.9 1984-09-01

Publications (2)

Publication Number Publication Date
KR860002571A true KR860002571A (ko) 1986-04-26
KR930002886B1 KR930002886B1 (ko) 1993-04-12

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KR1019850006323A KR930002886B1 (ko) 1984-09-01 1985-08-30 재조합 폴리펩티드의 분리방법

Country Status (12)

Country Link
EP (1) EP0173924B1 (ko)
JP (1) JPH078238B2 (ko)
KR (1) KR930002886B1 (ko)
AT (1) ATE49231T1 (ko)
DE (2) DE3432196A1 (ko)
DK (1) DK165748C (ko)
ES (1) ES8608038A1 (ko)
FI (1) FI85979C (ko)
HU (1) HU193806B (ko)
IE (1) IE58521B1 (ko)
IL (1) IL76264A (ko)
ZA (1) ZA856640B (ko)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196323A (en) * 1985-04-27 1993-03-23 Boehringer Ingelheim International Gmbh Process for preparing and purifying alpha-interferon
US4675387A (en) * 1985-07-26 1987-06-23 E. I. Du Pont De Nemours And Company Method for extracting protein with organic acid
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
US4912200A (en) * 1987-05-11 1990-03-27 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
EP2292637B1 (en) 2002-09-06 2016-01-06 Genentech, Inc. Process for protein extraction
JP4779537B2 (ja) * 2005-09-28 2011-09-28 パナソニック株式会社 電気掃除機

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1432039A (en) * 1972-11-10 1976-04-14 Dai Nippon Sugar Mfg Co Ltd Method of treating microorganisms
JPS5530834A (en) * 1978-08-25 1980-03-04 Nec Corp Method of forming ohmic contact in semiconductor pellet
JPS57118798A (en) * 1980-11-26 1982-07-23 Schering Plough Corp Obtaining of interferon solution
US4315852A (en) * 1980-11-26 1982-02-16 Schering Corporation Extraction of interferon from bacteria
WO1983002461A1 (en) * 1982-01-19 1983-07-21 Cetus Corp Multiclass hybrid interferons
WO1984002129A1 (en) * 1982-11-22 1984-06-07 Takeda Chemical Industries Ltd Human immune interferon protein and process for its preparation

Also Published As

Publication number Publication date
FI853324A0 (fi) 1985-08-30
HUT39471A (en) 1986-09-29
IE58521B1 (en) 1993-10-06
ZA856640B (en) 1987-05-27
DE3432196A1 (de) 1986-03-06
DE3575162D1 (de) 1990-02-08
IE852141L (en) 1986-03-01
KR930002886B1 (ko) 1993-04-12
EP0173924A3 (en) 1988-02-03
ATE49231T1 (de) 1990-01-15
FI85979B (fi) 1992-03-13
DK396685A (da) 1986-03-02
EP0173924B1 (de) 1990-01-03
IL76264A0 (en) 1986-01-31
IL76264A (en) 1990-02-09
DK396685D0 (da) 1985-08-30
FI85979C (fi) 1992-06-25
FI853324L (fi) 1986-03-02
EP0173924A2 (de) 1986-03-12
ES8608038A1 (es) 1986-06-01
ES546528A0 (es) 1986-06-01
HU193806B (en) 1987-12-28
DK165748C (da) 1993-06-14
DK165748B (da) 1993-01-11
JPH078238B2 (ja) 1995-02-01
JPS6170984A (ja) 1986-04-11

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