ZA856640B - New process for the disruption of bacteria cells containing recombinant polypeptides - Google Patents

New process for the disruption of bacteria cells containing recombinant polypeptides

Info

Publication number
ZA856640B
ZA856640B ZA856640A ZA856640A ZA856640B ZA 856640 B ZA856640 B ZA 856640B ZA 856640 A ZA856640 A ZA 856640A ZA 856640 A ZA856640 A ZA 856640A ZA 856640 B ZA856640 B ZA 856640B
Authority
ZA
South Africa
Prior art keywords
cells containing
disruption
new process
recombinant polypeptides
containing recombinant
Prior art date
Application number
ZA856640A
Inventor
Guenter Dr Adolf
Gerhard Prof Dr Bodo
Wolfgang Gibley
Kurt Konopitzky
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of ZA856640B publication Critical patent/ZA856640B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods

Abstract

1. Process for isolating a polypeptide prepared by recombinant methods from bacterial cells, characterised in that bacterial cells containing a polypeptide prepared by genetic engineering, these cells being suspended in an aqueous acid at a pH of from 2.0 to 4.0, are mechanically destroyed by shear forces and subsequently the polypeptide now present in the resulting suspension is separated and further purified by known methods.
ZA856640A 1984-09-01 1985-08-30 New process for the disruption of bacteria cells containing recombinant polypeptides ZA856640B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843432196 DE3432196A1 (en) 1984-09-01 1984-09-01 NEW MECHANICAL DIGESTIVE METHOD OF BACTERIAL CELLS FOR ISOLATING RECOMBINANTLY PRODUCED PEPTIDES

Publications (1)

Publication Number Publication Date
ZA856640B true ZA856640B (en) 1987-05-27

Family

ID=6244436

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA856640A ZA856640B (en) 1984-09-01 1985-08-30 New process for the disruption of bacteria cells containing recombinant polypeptides

Country Status (12)

Country Link
EP (1) EP0173924B1 (en)
JP (1) JPH078238B2 (en)
KR (1) KR930002886B1 (en)
AT (1) ATE49231T1 (en)
DE (2) DE3432196A1 (en)
DK (1) DK165748C (en)
ES (1) ES8608038A1 (en)
FI (1) FI85979C (en)
HU (1) HU193806B (en)
IE (1) IE58521B1 (en)
IL (1) IL76264A (en)
ZA (1) ZA856640B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196323A (en) * 1985-04-27 1993-03-23 Boehringer Ingelheim International Gmbh Process for preparing and purifying alpha-interferon
US4675387A (en) * 1985-07-26 1987-06-23 E. I. Du Pont De Nemours And Company Method for extracting protein with organic acid
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
US4912200A (en) * 1987-05-11 1990-03-27 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
US6967241B2 (en) 2002-09-06 2005-11-22 Genentech, Inc. Process for protein extraction
JP4779537B2 (en) * 2005-09-28 2011-09-28 パナソニック株式会社 Vacuum cleaner

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1432039A (en) * 1972-11-10 1976-04-14 Dai Nippon Sugar Mfg Co Ltd Method of treating microorganisms
JPS5530834A (en) * 1978-08-25 1980-03-04 Nec Corp Method of forming ohmic contact in semiconductor pellet
JPS57118798A (en) * 1980-11-26 1982-07-23 Schering Plough Corp Obtaining of interferon solution
US4315852A (en) * 1980-11-26 1982-02-16 Schering Corporation Extraction of interferon from bacteria
IT1167610B (en) * 1982-01-19 1987-05-13 Cetus Corp MULTICLASS HYBRID INTERFRERON, PHARMACEUTICAL COMPOSITION CONTAINING IT AND PRODUCTION PROCESS
WO1984002129A1 (en) * 1982-11-22 1984-06-07 Takeda Chemical Industries Ltd Human immune interferon protein and process for its preparation

Also Published As

Publication number Publication date
EP0173924B1 (en) 1990-01-03
ES546528A0 (en) 1986-06-01
IE58521B1 (en) 1993-10-06
JPH078238B2 (en) 1995-02-01
DE3432196A1 (en) 1986-03-06
FI853324L (en) 1986-03-02
DK396685A (en) 1986-03-02
EP0173924A3 (en) 1988-02-03
DK165748C (en) 1993-06-14
ES8608038A1 (en) 1986-06-01
DK396685D0 (en) 1985-08-30
KR860002571A (en) 1986-04-26
HUT39471A (en) 1986-09-29
HU193806B (en) 1987-12-28
IL76264A (en) 1990-02-09
DE3575162D1 (en) 1990-02-08
JPS6170984A (en) 1986-04-11
ATE49231T1 (en) 1990-01-15
FI85979C (en) 1992-06-25
EP0173924A2 (en) 1986-03-12
IE852141L (en) 1986-03-01
DK165748B (en) 1993-01-11
KR930002886B1 (en) 1993-04-12
IL76264A0 (en) 1986-01-31
FI85979B (en) 1992-03-13
FI853324A0 (en) 1985-08-30

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