FI853324A0 - NYTT MEKANISKT BEHANDLINGSFOERFARANDE FOER ISOLERING AV BAKTERIECELLER FRAON REKOMBINAT FRAMSTAELLDA PEPTIDER. - Google Patents

NYTT MEKANISKT BEHANDLINGSFOERFARANDE FOER ISOLERING AV BAKTERIECELLER FRAON REKOMBINAT FRAMSTAELLDA PEPTIDER.

Info

Publication number
FI853324A0
FI853324A0 FI853324A FI853324A FI853324A0 FI 853324 A0 FI853324 A0 FI 853324A0 FI 853324 A FI853324 A FI 853324A FI 853324 A FI853324 A FI 853324A FI 853324 A0 FI853324 A0 FI 853324A0
Authority
FI
Finland
Prior art keywords
rekombinat
mekaniskt
behandlingsfoerfarande
bakterieceller
framstaellda
Prior art date
Application number
FI853324A
Other languages
Finnish (fi)
Other versions
FI85979C (en
FI85979B (en
FI853324L (en
Inventor
Guenther Adolf
Gerhard Bodo
Wolfgang Gibley
Kurt Konopitzky
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Publication of FI853324A0 publication Critical patent/FI853324A0/en
Publication of FI853324L publication Critical patent/FI853324L/en
Application granted granted Critical
Publication of FI85979B publication Critical patent/FI85979B/en
Publication of FI85979C publication Critical patent/FI85979C/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1. Process for isolating a polypeptide prepared by recombinant methods from bacterial cells, characterised in that bacterial cells containing a polypeptide prepared by genetic engineering, these cells being suspended in an aqueous acid at a pH of from 2.0 to 4.0, are mechanically destroyed by shear forces and subsequently the polypeptide now present in the resulting suspension is separated and further purified by known methods.
FI853324A 1984-09-01 1985-08-30 FOERFARANDE FOER FRAMSTAELLNING AV ETT REKOMBINANTFRAMSTAELLT INTERFERON UR BAKTERIECELLER. FI85979C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3432196 1984-09-01
DE19843432196 DE3432196A1 (en) 1984-09-01 1984-09-01 NEW MECHANICAL DIGESTIVE METHOD OF BACTERIAL CELLS FOR ISOLATING RECOMBINANTLY PRODUCED PEPTIDES

Publications (4)

Publication Number Publication Date
FI853324A0 true FI853324A0 (en) 1985-08-30
FI853324L FI853324L (en) 1986-03-02
FI85979B FI85979B (en) 1992-03-13
FI85979C FI85979C (en) 1992-06-25

Family

ID=6244436

Family Applications (1)

Application Number Title Priority Date Filing Date
FI853324A FI85979C (en) 1984-09-01 1985-08-30 FOERFARANDE FOER FRAMSTAELLNING AV ETT REKOMBINANTFRAMSTAELLT INTERFERON UR BAKTERIECELLER.

Country Status (12)

Country Link
EP (1) EP0173924B1 (en)
JP (1) JPH078238B2 (en)
KR (1) KR930002886B1 (en)
AT (1) ATE49231T1 (en)
DE (2) DE3432196A1 (en)
DK (1) DK165748C (en)
ES (1) ES8608038A1 (en)
FI (1) FI85979C (en)
HU (1) HU193806B (en)
IE (1) IE58521B1 (en)
IL (1) IL76264A (en)
ZA (1) ZA856640B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196323A (en) * 1985-04-27 1993-03-23 Boehringer Ingelheim International Gmbh Process for preparing and purifying alpha-interferon
US4675387A (en) * 1985-07-26 1987-06-23 E. I. Du Pont De Nemours And Company Method for extracting protein with organic acid
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
US4912200A (en) * 1987-05-11 1990-03-27 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
EP1539798B1 (en) 2002-09-06 2010-11-24 Genentech, Inc. Process for protein extraction
JP4779537B2 (en) * 2005-09-28 2011-09-28 パナソニック株式会社 Vacuum cleaner

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1432039A (en) * 1972-11-10 1976-04-14 Dai Nippon Sugar Mfg Co Ltd Method of treating microorganisms
JPS5530834A (en) * 1978-08-25 1980-03-04 Nec Corp Method of forming ohmic contact in semiconductor pellet
US4315852A (en) * 1980-11-26 1982-02-16 Schering Corporation Extraction of interferon from bacteria
JPS57118798A (en) * 1980-11-26 1982-07-23 Schering Plough Corp Obtaining of interferon solution
IT1167610B (en) * 1982-01-19 1987-05-13 Cetus Corp MULTICLASS HYBRID INTERFRERON, PHARMACEUTICAL COMPOSITION CONTAINING IT AND PRODUCTION PROCESS
WO1984002129A1 (en) * 1982-11-22 1984-06-07 Takeda Chemical Industries Ltd Human immune interferon protein and process for its preparation

Also Published As

Publication number Publication date
EP0173924A3 (en) 1988-02-03
JPS6170984A (en) 1986-04-11
KR930002886B1 (en) 1993-04-12
HUT39471A (en) 1986-09-29
JPH078238B2 (en) 1995-02-01
ES8608038A1 (en) 1986-06-01
DK165748B (en) 1993-01-11
IE852141L (en) 1986-03-01
IE58521B1 (en) 1993-10-06
FI85979C (en) 1992-06-25
FI85979B (en) 1992-03-13
IL76264A (en) 1990-02-09
DK396685D0 (en) 1985-08-30
DK165748C (en) 1993-06-14
HU193806B (en) 1987-12-28
ZA856640B (en) 1987-05-27
ES546528A0 (en) 1986-06-01
DE3432196A1 (en) 1986-03-06
IL76264A0 (en) 1986-01-31
EP0173924A2 (en) 1986-03-12
FI853324L (en) 1986-03-02
DK396685A (en) 1986-03-02
ATE49231T1 (en) 1990-01-15
KR860002571A (en) 1986-04-26
EP0173924B1 (en) 1990-01-03
DE3575162D1 (en) 1990-02-08

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Legal Events

Date Code Title Description
MM Patent lapsed
MM Patent lapsed

Owner name: BOEHRINGER INGELHEIM INTERNATIONAL GMBH