KR850001279A - α-아밀라제의 제법 - Google Patents

α-아밀라제의 제법 Download PDF

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Publication number
KR850001279A
KR850001279A KR1019840004081A KR840004081A KR850001279A KR 850001279 A KR850001279 A KR 850001279A KR 1019840004081 A KR1019840004081 A KR 1019840004081A KR 840004081 A KR840004081 A KR 840004081A KR 850001279 A KR850001279 A KR 850001279A
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KR
South Korea
Prior art keywords
enzyme
amylase
atcc
clostridium spp
microorganism
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KR1019840004081A
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English (en)
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KR890001827B1 (ko
Inventor
엠 카트코신 데니이스 (외 2)
Original Assignee
엘런 피이 트리버스
시이 피이 시이 인터내쇼날 인코포레이팃드
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Application filed by 엘런 피이 트리버스, 시이 피이 시이 인터내쇼날 인코포레이팃드 filed Critical 엘런 피이 트리버스
Publication of KR850001279A publication Critical patent/KR850001279A/ko
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Publication of KR890001827B1 publication Critical patent/KR890001827B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/842—Clostridium

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Developing Agents For Electrophotography (AREA)
  • Lubrication Of Internal Combustion Engines (AREA)

Abstract

내용 없음

Description

α-아밀라제의 제법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. 클로스트리듐 종ATCC 39, 251, ATCC 39,252 그의 돌연변이 균주 또는 α-아밀라제효소 생성의 암호인 유전정보를 상기클로스트리듐종미생물로 부터 받은 미생물로 구성된 군으로 부터 선택된 미생물로 부터 유도된 α-아밀라제.
  2. 제1항에 있어서 SDS-폴리아크릴아미드겔 전기영동법에 의해 측정된 분자량이 약 75000±3000인 효소.
  3. 제1항에 있어서 5mMCa++존재하 PH 4.2, 60℃에서의 반감기가 70시간 이상인 요소.
  4. 제1항에 있어서 PH 약 5.0에서 최대 α-아밀라제 활성을 갖는 효소.
  5. 제1항에 있어서 PH 4.5, 85℃에서 최대 α-아밀라제 활성을 갖는 효소.
  6. 클로스트리듐 종ATCC 39, 251, ATCC 39, 252, 그의 돌연변이균주 또는 α-아밀라제 효소생성의 암호인 유전정보를 클로스트리듐 종 미생물로 부터 받은 미생물로 구성된 군으로부터 미생물을 선택한 후, 선택된 미생물세포를 영양배지 내에서 배양하고 배지로부터 α-아밀라제 효소를 단리시키는 것으로 구성된 α-아밀라제효소를 제조하는 방법.
  7. 전분의 수성슬러리 또는 용액을 pH 3.5-7.0에서 제1항의 α-아밀라제효소로 전분 가수분해물 용액이 생성되기에 충분한 시간 동안 처리하는 것으로 구성된 전분을 가수분해하는 방법.
  8. 제7항에 있어서 온도 약 50-100℃, pH 약 4.0-6.0에서 전환을 수행하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840004081A 1983-07-13 1984-07-12 α-아밀라제의제법 KR890001827B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/513,517 US4578352A (en) 1983-07-13 1983-07-13 Novel thermostable, aciduric alpha-amylase and method for its production
US513517 1983-07-13

Publications (2)

Publication Number Publication Date
KR850001279A true KR850001279A (ko) 1985-03-18
KR890001827B1 KR890001827B1 (ko) 1989-05-25

Family

ID=24043610

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019840004081A KR890001827B1 (ko) 1983-07-13 1984-07-12 α-아밀라제의제법

Country Status (16)

Country Link
US (1) US4578352A (ko)
EP (1) EP0131253B1 (ko)
JP (1) JPS6041482A (ko)
KR (1) KR890001827B1 (ko)
AT (1) ATE31077T1 (ko)
AU (1) AU576505B2 (ko)
CA (1) CA1221325A (ko)
DE (1) DE3467771D1 (ko)
DK (1) DK343284A (ko)
ES (2) ES534245A0 (ko)
FI (1) FI842707A (ko)
GB (1) GB2143241B (ko)
MY (1) MY101216A (ko)
NZ (1) NZ208298A (ko)
YU (1) YU120484A (ko)
ZA (1) ZA844401B (ko)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0184019B1 (en) * 1984-11-09 1991-03-06 Hitachi, Ltd. Thermostable alpha-amylase-producing, thermophilic anaerobic bacteria, thermostable alpha-amylase and process for producing the same
JPS61115491A (ja) * 1984-11-09 1986-06-03 Hitachi Ltd 耐熱性α−アミラ−ゼ及びその製造方法
JPS61115496A (ja) * 1984-11-09 1986-06-03 Hitachi Ltd 殿粉の液化方法
US4613570A (en) * 1985-02-08 1986-09-23 Cpc International Inc. Novel thermostable, aciduric alpha-amylase and method for its production
JPH0683668B2 (ja) * 1985-07-16 1994-10-26 株式会社日立製作所 耐熱性α−アミラ−ゼの製造方法
JPS62283988A (ja) * 1986-06-02 1987-12-09 Hitachi Ltd マルトオリゴ糖の製造方法
JPS6342695A (ja) * 1986-08-08 1988-02-23 Hitachi Ltd 糖の製造方法
DE3639267A1 (de) * 1986-11-17 1988-09-22 Antranikian Garabed Verfahren zur foerderung der exkretion von amylolytischen enzymen aus bakterien sowie nach dem verfahren erhaltene enzyme
DE3702626A1 (de) * 1987-01-29 1989-01-05 Antranikian Gerabed Thermostabile amylasen und pullulanasen aus 2 anaeroben mikroorganismen
DE3712051A1 (de) * 1987-04-09 1988-10-27 Antranikian Garabed Thermostabile amylasen und pullulanasen aus 2 anaeroben mikroorganismen
US5188956A (en) * 1988-07-01 1993-02-23 Showa Denka K.K. Thermostable amylase
US5010008A (en) * 1988-12-12 1991-04-23 Enzyme Bio-Systems Ltd. Stable liquid enzyme concentrate and process for its production
GB2291058B (en) * 1994-07-14 1998-12-23 Solvay Acid-stable and thermo-stable alpha-amylases derived from sufolobus species
US6391598B1 (en) * 1999-03-29 2002-05-21 Board Of Regents Of The University Of Nebraska Preparation of fatty acid metal salts and enzymes from ruminal fluid
WO2014122449A1 (en) 2013-02-05 2014-08-14 Green Biologics Ltd Cyclodextrin glucanotransferase
CN108823185A (zh) * 2018-06-25 2018-11-16 安徽新熙盟生物科技有限公司 高酶活发酵液的培养方法及提取耐酸α-淀粉酶的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR208286A1 (es) * 1973-04-10 1976-12-20 Cpc International Inc Procedimiento para convertir directamente almidon granular en un hidrolizado de almidon soluble
US4284722A (en) * 1978-08-16 1981-08-18 Cpc International Inc. Heat and acid-stable alpha-amylase enzymes and processes for producing the same
IL61982A (en) * 1980-02-15 1984-01-31 Cpc International Inc Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes
CA1170202A (en) * 1981-01-15 1984-07-03 Susan Mickel Process for cloning the gene coding for a thermostable alpha-amylase into escherichia coli and bacillus subtilis

Also Published As

Publication number Publication date
ES8506346A1 (es) 1985-07-01
EP0131253B1 (en) 1987-11-25
DK343284D0 (da) 1984-07-12
ES538508A0 (es) 1985-11-01
AU576505B2 (en) 1988-09-01
AU3031184A (en) 1985-01-17
DK343284A (da) 1985-01-14
US4578352A (en) 1986-03-25
EP0131253A1 (en) 1985-01-16
ZA844401B (en) 1985-01-30
JPS6041482A (ja) 1985-03-05
GB2143241A (en) 1985-02-06
KR890001827B1 (ko) 1989-05-25
YU120484A (en) 1986-06-30
MY101216A (en) 1991-08-17
GB8417767D0 (en) 1984-08-15
FI842707A0 (fi) 1984-07-05
ATE31077T1 (de) 1987-12-15
DE3467771D1 (en) 1988-01-07
GB2143241B (en) 1988-04-20
ES8602116A1 (es) 1985-11-01
ES534245A0 (es) 1985-07-01
FI842707A (fi) 1985-01-14
NZ208298A (en) 1988-06-30
CA1221325A (en) 1987-05-05

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