ES8504245A1 - Procedimiento para la obtencion de sarcosin-oxidasa - Google Patents

Procedimiento para la obtencion de sarcosin-oxidasa

Info

Publication number
ES8504245A1
ES8504245A1 ES534117A ES534117A ES8504245A1 ES 8504245 A1 ES8504245 A1 ES 8504245A1 ES 534117 A ES534117 A ES 534117A ES 534117 A ES534117 A ES 534117A ES 8504245 A1 ES8504245 A1 ES 8504245A1
Authority
ES
Spain
Prior art keywords
sarcosine oxidase
obtaining
sarcosine
oxidase
biomass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534117A
Other languages
English (en)
Other versions
ES534117A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of ES8504245A1 publication Critical patent/ES8504245A1/es
Publication of ES534117A0 publication Critical patent/ES534117A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE SARCOSIN-OXIDASA.COMPRENDE: A) DISGREGAR MECANICAMENTE LA MASA DE MICROORGANISMOS EN UNA SOLUCION TAMPON DE PH 6 A 9,5 QUE CONTIENE 0,1 A 3 EN PESO DE POLIETILENIMINA; B) DILUIR CON AGUA LA PORCION SOBRANTE HASTA CONSEGUIR UN 0,05 DE POLIETILENIMINA PARA CBTENER UN PRECIPITADO; C) DISOLVER EL PRECIPITADO EN EL TAMPON Y FRACCIONARLO A TRAVES DE FENIL-SEPHAROSE HIDROFOBO; D) TRATAR LA FRACCION ACTIVA CON SULFATO AMONICO OBTENIENDOSE LA FRACCION INSOLUBLE EN SULFATO AMONICO ENTRE 1,8 Y 3,2 M; Y C) SOMETER A UN FRACCIONAMIENTO EN TAMIZ MOLECULAR Y A UNA CROMATOGRAFIA A TRAVES DE OCTIL-SEPHAROSE, A LA FRACCION INSOLUBLE EN AMONIACO.
ES534117A 1983-07-26 1984-07-06 Procedimiento para la obtencion de sarcosin-oxidasa Granted ES534117A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19833326888 DE3326888A1 (de) 1983-07-26 1983-07-26 Sarcosin-oxidase

Publications (2)

Publication Number Publication Date
ES8504245A1 true ES8504245A1 (es) 1985-04-16
ES534117A0 ES534117A0 (es) 1985-04-16

Family

ID=6204937

Family Applications (1)

Application Number Title Priority Date Filing Date
ES534117A Granted ES534117A0 (es) 1983-07-26 1984-07-06 Procedimiento para la obtencion de sarcosin-oxidasa

Country Status (7)

Country Link
US (1) US5024945A (es)
EP (1) EP0135070B1 (es)
JP (1) JPS6043379A (es)
AT (1) ATE33148T1 (es)
CA (1) CA1230301A (es)
DE (2) DE3326888A1 (es)
ES (1) ES534117A0 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3515586A1 (de) * 1985-04-30 1986-11-06 Boehringer Mannheim Gmbh, 6800 Mannheim Stabilisiertes sarcosinoxidase-praeparat
DE3519218A1 (de) * 1985-05-29 1986-12-04 Boehringer Mannheim Gmbh, 6800 Mannheim H(pfeil abwaerts)2(pfeil abwaerts)o(pfeil abwaerts)2(pfeil abwaerts)-bildende sarcosinoxidase, ihre herstellung und verwendung
DE69625981T2 (de) * 1995-10-13 2004-01-22 Ajinomoto Co., Inc. Verfahren zum Entfernen von Zellen aus Fermentationsbrühe
EP0770676A3 (en) * 1995-10-23 1999-05-19 Ajinomoto Co., Ltd. Method for treating fermentation broth
JP3599472B2 (ja) * 1996-03-21 2004-12-08 オリヱント化学工業株式会社 黒色ポリアミド樹脂組成物
US5827911A (en) * 1996-04-23 1998-10-27 Orient Chemical Industries, Ltd. Colored thermoplastic resin composition
KR100611546B1 (ko) 1998-11-02 2006-08-10 오리엔트 가가쿠 고교 가부시키가이샤 착색 열가소성 수지 조성물 및 그의 관련 기술
JP3757081B2 (ja) 1999-06-30 2006-03-22 オリヱント化学工業株式会社 水不溶性ニグロシン及びその関連技術
US7229812B2 (en) 2002-11-13 2007-06-12 Toyo Boseki Kabushiki Kaisha Modified sarcosine oxidase, process for producing the same and reagent composition using the same
JP4405324B2 (ja) * 2003-11-18 2010-01-27 キッコーマン株式会社 改変型ザルコシンオキシダーゼ、改変型ザルコシンオキシダーゼ遺伝子及び改変型ザルコシンオキシダーゼの製造法
CN109715799B (zh) 2016-09-15 2023-04-04 龟甲万株式会社 经修饰的肌氨酸氧化酶及其基因和制造方法
CN110029094B (zh) * 2019-03-01 2020-11-17 武汉生之源生物科技股份有限公司 一种突变的肌氨酸氧化酶及其在肌酐检测中的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2001902C3 (de) * 1970-01-16 1978-10-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Reinigung und Fraktionierung von gelösten aktiven Proteinen
JPS6029473B2 (ja) * 1977-10-04 1985-07-10 東洋醸造株式会社 ザルコシン・オキシダ−ゼの製法
US4461833A (en) * 1982-06-23 1984-07-24 University Patents, Inc. Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract

Also Published As

Publication number Publication date
DE3326888A1 (de) 1985-02-14
EP0135070A2 (de) 1985-03-27
US5024945A (en) 1991-06-18
JPS6043379A (ja) 1985-03-07
DE3470060D1 (en) 1988-04-28
ATE33148T1 (de) 1988-04-15
ES534117A0 (es) 1985-04-16
CA1230301A (en) 1987-12-15
EP0135070A3 (en) 1985-05-08
EP0135070B1 (de) 1988-03-23
JPS6219153B2 (es) 1987-04-27

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20040601