JPS6460376A - Amylase having pullulanase activity and production thereof - Google Patents
Amylase having pullulanase activity and production thereofInfo
- Publication number
- JPS6460376A JPS6460376A JP21550787A JP21550787A JPS6460376A JP S6460376 A JPS6460376 A JP S6460376A JP 21550787 A JP21550787 A JP 21550787A JP 21550787 A JP21550787 A JP 21550787A JP S6460376 A JPS6460376 A JP S6460376A
- Authority
- JP
- Japan
- Prior art keywords
- optimum
- stable
- amylase
- starch
- pullulan
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
NEW MATERIAL:The amylase having the following physical and chemical properties. Action and substrate specificity, acting to soluble starch or starch-based sugar and pullulan and scarcely acting to raw starch, dextran, maltotetraose, maltose and isomaltose; optimum pH and stable pH; optimum pH to soluble starch is 7.0-8.5 and the stable pH is 5.0-9.0, optimum pH to pullulan is 7.0 and the stable pH is 5.0-8.0; optimum temperature and heat-stability, optimum active temperature is 50 deg.C, stable up to 40 deg.C, inactivated at 60 deg.C in 30min; isoelectric point, 4.13 by electrophoresis; molecular weight, 218,000 by SDS-PAGE process. USE:Production of malto-oligosaccharide. PREPARATION:The objective amylase having pullulanase activity can be produced e.g. by culturing Bacillus circulans F-2 (FERM P-7361) belonging to Bacillus genus in a medium, separating the bacterial cells from the cultured product, collecting a pullulanase-active fraction from the supernatant liquid and subjecting the fraction to extraction, salting-out, ion exchange treatment and purification by hydrophobic chromatography.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP21550787A JPH0789918B2 (en) | 1987-08-31 | 1987-08-31 | Amylase having pullulanase activity and method for producing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP21550787A JPH0789918B2 (en) | 1987-08-31 | 1987-08-31 | Amylase having pullulanase activity and method for producing the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6460376A true JPS6460376A (en) | 1989-03-07 |
JPH0789918B2 JPH0789918B2 (en) | 1995-10-04 |
Family
ID=16673548
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP21550787A Expired - Lifetime JPH0789918B2 (en) | 1987-08-31 | 1987-08-31 | Amylase having pullulanase activity and method for producing the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0789918B2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0418835A1 (en) | 1989-09-19 | 1991-03-27 | Kao Corporation | Novel alkaline pullulanase y having alpha-amylase activity, microorganism producing the same, and process for producing the same |
-
1987
- 1987-08-31 JP JP21550787A patent/JPH0789918B2/en not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0418835A1 (en) | 1989-09-19 | 1991-03-27 | Kao Corporation | Novel alkaline pullulanase y having alpha-amylase activity, microorganism producing the same, and process for producing the same |
Also Published As
Publication number | Publication date |
---|---|
JPH0789918B2 (en) | 1995-10-04 |
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