FI116965B - Enzymbaserat tillsatsämne, djurfoder innehållande detsamma och förfarande för framställning av ett enzymbaserat tillsatsämne - Google Patents

Enzymbaserat tillsatsämne, djurfoder innehållande detsamma och förfarande för framställning av ett enzymbaserat tillsatsämne Download PDF

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Publication number
FI116965B
FI116965B FI953867A FI953867A FI116965B FI 116965 B FI116965 B FI 116965B FI 953867 A FI953867 A FI 953867A FI 953867 A FI953867 A FI 953867A FI 116965 B FI116965 B FI 116965B
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FI
Finland
Prior art keywords
ser
thr
gly
asn
enzyme
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Application number
FI953867A
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English (en)
Finnish (fi)
Other versions
FI953867A0 (sv
FI953867A (sv
Inventor
Michael Ward
Kathleen A Clarkson
Edmund Larenas
Andrew John Morgan
Timothy Fowler
Michael Richard Bedford
Katherine D Collier
Original Assignee
Genencor Int
Finnfeeds Int Ltd
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22617873&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI116965(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor Int, Finnfeeds Int Ltd filed Critical Genencor Int
Publication of FI953867A0 publication Critical patent/FI953867A0/sv
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Publication of FI116965B publication Critical patent/FI116965B/sv

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Detergent Compositions (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (7)

1. Enzymbaserat fodertillsatsämne, k ä tecknatav att det innehäller 5 (i) en eller flera av de följande: (a) en< nas EGI vilket saknar en cellulosa bindande domän, doglucanas EGII vilket saknar en cellulosa bindande och (c) en aktiv cellulaskärna av en endoglucanas punkt (a) eller (b), varvid EGI hänvisar tili ett < 10 canas, som härstammar frän en Trichoderma-art med optimum av ca 4,0 - 6,0, en isoelektrisk punkt (pl 4,5 - 4,7 och en molekylvikt av ca 47 - 49 kilodali EGII hänvisar tili ett endoglucanas, som härstamn en Trichoderma-art med pH-optimum av ca 4,0 - 6,0, i 15 ca 5,5 och en molekylvikt av ca 35 kilodalton; och (ii) 0-20 vikt-% av ett cellobiohydrolas nat pä halten av cellulasproteiner i fodertillsatsä
2. Enzymbaserat fodertillsatsämne enligt krav 1, kännetecknatav att det inte ai] 20 häller cellobiohydrolas.
3. Enzymbaserat fodertillsatsämne enligt krav 1 eller 2, kännetecknatav att fo< : satsämnet ytterligare innehäller ätminstone ett el] ·1· :Yj ra andra enzymer, vilka väljs bland ett xylanas, e :v. 25 teas, ett a-amylas, ett glucoamylas, ett lipas, ett * nas, ett mannanas, ett α-galaktosidas, ett a-arab: * 1 i nosidas och ett fytas. * · · * 4. Enzymbaserat fodertillsatsämne enligt krav 3, kännetecknatav att xylanaset ä2 *···1 30 longibrachiatum xylanas med ett högt pl-värde oc ** · _· ö-h-h lÄrrl" nT-värrlp. 79
6. Spannmälsbaserat djurfoder, kannet n a t av att det innehäller ätminstone ett spanni väljs bland korn, väte, rägväte, räg och majs, och zymbaserat fodertillsatsämne enligt nagot av fö 5 patentkrav.
7. Spannmälsbaserat djurfoder enligt paten kännetecknat av att spannmälet är at; korn.
8. Förfarande för framställning av ett en 10 rat fodertillsatsämne enligt nägot av patentkraver kännetecknat av att det innehäller ett vilket man bildar gentekniskt frän en Trichoderma-genetiskt modifierad stam, vilken producerar önska< mer i lämpliga relativa mängder, odlar den genetis 15 fierade stammen och tillvaratar tillsatsämnet frän oc
9. Förfarande enligt patentkrav 8, k ä tecknat av att den genetiskt modifierade härstammar frän Γ. longibrachiatum, T. reesei elle ri.de, vars genom har modifierats sä att det produi 20 ett anrikat sätt ett eller flera endoglucanaser o det är inkapabelt att producera ett eller flera * nelit aktiva cellobiohydrolaser. : 10. Förfarande enligt patentkrav 9, k ä ;V* tecknat av att genomen av den genetiskt mod • * 25 stammen är inkapabel att bilda funktionellt akt • och/eller EGII. » * 5 *·* * • *· • · « • « « « i · · • * ··· • *
FI953867A 1993-12-17 1995-08-16 Enzymbaserat tillsatsämne, djurfoder innehållande detsamma och förfarande för framställning av ett enzymbaserat tillsatsämne FI116965B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/169,948 US5861271A (en) 1993-12-17 1993-12-17 Cellulase enzymes and systems for their expressions
US16994893 1993-12-17
PCT/EP1994/004212 WO1995016360A1 (en) 1993-12-17 1994-12-19 An enzyme feed additive and animal feed including it
EP9404212 1994-12-19

Publications (3)

Publication Number Publication Date
FI953867A0 FI953867A0 (sv) 1995-08-16
FI953867A FI953867A (sv) 1995-10-03
FI116965B true FI116965B (sv) 2006-04-28

Family

ID=22617873

Family Applications (2)

Application Number Title Priority Date Filing Date
FI953867A FI116965B (sv) 1993-12-17 1995-08-16 Enzymbaserat tillsatsämne, djurfoder innehållande detsamma och förfarande för framställning av ett enzymbaserat tillsatsämne
FI962444A FI119695B (sv) 1993-12-17 1996-06-12 Nya cellulasenzymer och system för expression av dem

Family Applications After (1)

Application Number Title Priority Date Filing Date
FI962444A FI119695B (sv) 1993-12-17 1996-06-12 Nya cellulasenzymer och system för expression av dem

Country Status (16)

Country Link
US (3) US5861271A (sv)
EP (3) EP1700524B1 (sv)
JP (1) JPH09506514A (sv)
CN (1) CN1093379C (sv)
AT (3) ATE329039T1 (sv)
AU (1) AU685210B2 (sv)
CA (2) CA2156066C (sv)
DE (3) DE69427812T2 (sv)
DK (2) DK0684770T3 (sv)
ES (1) ES2160694T3 (sv)
FI (2) FI116965B (sv)
HK (1) HK1097163A1 (sv)
NO (1) NO314562B1 (sv)
NZ (1) NZ278373A (sv)
PT (1) PT684770E (sv)
WO (2) WO1995016360A1 (sv)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8715647B2 (en) 1993-12-17 2014-05-06 Danisco Us Inc. Enzyme feed additive and animal feed including it

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DE69435078D1 (de) 2008-04-10
ES2160694T3 (es) 2001-11-16
ATE387104T1 (de) 2008-03-15
DE69435078T2 (de) 2009-03-26
EP1700524A1 (en) 2006-09-13
DE69434759D1 (de) 2006-07-20
AU1383695A (en) 1995-07-03
CA2156066C (en) 2005-07-12
EP0733115A1 (en) 1996-09-25
US6562340B1 (en) 2003-05-13
JPH09506514A (ja) 1997-06-30
FI119695B (sv) 2009-02-13
EP1700524B1 (en) 2008-02-27
HK1097163A1 (en) 2007-06-22
CA2156066A1 (en) 1995-06-22
EP0684770A1 (en) 1995-12-06
NO953218L (no) 1995-10-16
DE69427812D1 (de) 2001-08-30
US5874276A (en) 1999-02-23
EP0733115B1 (en) 2006-06-07
FI962444A0 (sv) 1996-06-12
EP0684770B1 (en) 2001-07-25
FI953867A0 (sv) 1995-08-16
FI962444A (sv) 1996-06-12
NZ278373A (en) 1997-10-24
NO953218D0 (no) 1995-08-16
ATE203373T1 (de) 2001-08-15
DE69434759T2 (de) 2007-05-16
ATE329039T1 (de) 2006-06-15
CN1093379C (zh) 2002-10-30
DK0684770T3 (da) 2001-09-17
US5861271A (en) 1999-01-19
CA2178636A1 (en) 1995-06-22
PT684770E (pt) 2001-11-30
CN1118131A (zh) 1996-03-06
DK0733115T3 (da) 2006-10-09
NO314562B1 (no) 2003-04-14
WO1995016360A1 (en) 1995-06-22
FI953867A (sv) 1995-10-03
DE69427812T2 (de) 2002-06-06
WO1995016782A1 (en) 1995-06-22
AU685210B2 (en) 1998-01-15

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