DK2314668T3 - Gen-inaktiverede mutanter med ændret proteinproduktion - Google Patents

Gen-inaktiverede mutanter med ændret proteinproduktion Download PDF

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Publication number
DK2314668T3
DK2314668T3 DK10188507.7T DK10188507T DK2314668T3 DK 2314668 T3 DK2314668 T3 DK 2314668T3 DK 10188507 T DK10188507 T DK 10188507T DK 2314668 T3 DK2314668 T3 DK 2314668T3
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Denmark
Prior art keywords
gen
protein production
inactivated mutants
changed protein
changed
Prior art date
Application number
DK10188507.7T
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English (en)
Inventor
Huaming Wang
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Danisco Us Inc
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Publication of DK2314668T3 publication Critical patent/DK2314668T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0055Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061Laccase (1.10.3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y110/00Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002Laccase (1.10.3.2)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK10188507.7T 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion DK2314668T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67041505P 2005-04-12 2005-04-12
EP09005625.0A EP2123746B1 (en) 2005-04-12 2006-04-11 Gene inactivated mutants with altered protein production

Publications (1)

Publication Number Publication Date
DK2314668T3 true DK2314668T3 (da) 2017-02-13

Family

ID=36954411

Family Applications (4)

Application Number Title Priority Date Filing Date
DK09005625.0T DK2123746T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion
DK10188484.9T DK2314667T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion
DK10188507.7T DK2314668T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion
DK10188499.7T DK2333045T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DK09005625.0T DK2123746T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion
DK10188484.9T DK2314667T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK10188499.7T DK2333045T3 (da) 2005-04-12 2006-04-11 Gen-inaktiverede mutanter med ændret proteinproduktion

Country Status (5)

Country Link
US (3) US7691621B2 (da)
EP (5) EP2333045B1 (da)
CN (1) CN101146907B (da)
DK (4) DK2123746T3 (da)
WO (1) WO2006110677A2 (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8389269B2 (en) * 2006-11-02 2013-03-05 Dsm Ip Assets B.V. Production of secreted proteins by filamentous fungi
WO2008073914A2 (en) * 2006-12-10 2008-06-19 Dyadic International Inc. Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
WO2008081031A2 (en) * 2007-01-05 2008-07-10 Novozymes A/S Methods of increasing the production yield of a secreted antibody in a filamentous fungus by overexpression of the chaperone bip
CN101883848A (zh) 2007-10-01 2010-11-10 诺维信公司 具有蛋白酶活性的多肽和编码该多肽的多核苷酸
US20090253173A1 (en) * 2008-04-08 2009-10-08 Danisco Us Inc., Genencor Division Filamentous fungi with inactivated protease genes for altered protein production
CA2732104A1 (en) * 2008-07-29 2010-02-04 Danisco Us Inc. Increased production of aspartic proteases in filamentous fungal cells
US20120149064A1 (en) * 2008-11-18 2012-06-14 Danisco Us Inc. Filamentous Fungi With Impaired PTRB Activity For Altered Protein Production
JP2012533312A (ja) * 2009-07-22 2012-12-27 ディーエスエム アイピー アセッツ ビー.ブイ. 目的の化合物を産生するための改善された宿主細胞
WO2012048334A2 (en) * 2010-10-08 2012-04-12 Dyadic International (Usa) Inc. Novel fungal proteases
WO2013048661A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
BR112014016587A2 (pt) 2012-01-05 2020-10-27 Novartis Ag células fúngicas filamentosas deficientes de protease e métodos de uso das mesmas
WO2013174927A1 (en) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production of fucosylated glycoproteins
MX2016000306A (es) 2013-07-10 2016-08-08 Novartis Ag Celulas de hongos filamentosos deficientes en multiples proteasas y métodos de uso de las mismas.
EP3036324B1 (en) * 2013-08-23 2018-12-19 Novozymes A/S Regulated pepc expression
WO2015054507A1 (en) * 2013-10-10 2015-04-16 Pronutria, Inc. Nutritive polypeptide production systems, and methods of manufacture and use thereof
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
WO2018093752A1 (en) * 2016-11-15 2018-05-24 Danisco Us Inc. Filamentous fungi with improved protein production
WO2023148301A1 (en) 2022-02-02 2023-08-10 Biotalys NV Modified microbial cells and uses thereof

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US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
CA1338400C (en) 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0625577A1 (en) 1985-08-29 1994-11-23 Genencor International, Inc. Heterologous polypeptides expressed in filamentous fungi, processes for their preparation, and vectors for their preparation
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
WO1992010581A1 (en) 1990-12-10 1992-06-25 Genencor International, Inc. IMPROVED SACCHARIFICATION OF CELLULOSE BY CLONING AND AMPLIFICATION OF THE β-GLUCOSIDASE GENE OF TRICHODERMA REESEI
US5846802A (en) * 1992-04-15 1998-12-08 Buxton; Frank Fungal Protease
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
JP2000501620A (ja) * 1995-12-15 2000-02-15 ノボ ノルディスク アクティーゼルスカブ areA,pepC及び/又はpepE遺伝子が不活性化されている菌類
EP0897012A1 (en) * 1997-07-05 1999-02-17 Societe Des Produits Nestle S.A. Cloning of the prolyl-dipeptidyl-peptidase from aspergillus oryzae
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
CN100582226C (zh) * 2001-02-23 2010-01-20 Dsmip资产有限公司 编码新蛋白水解酶的新基因
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EP2292641B1 (en) * 2003-11-06 2017-12-06 Danisco US Inc. Expression in filamentous fungi of protease inhibitors and variants thereof

Also Published As

Publication number Publication date
EP2314668A3 (en) 2011-12-14
EP2314667A2 (en) 2011-04-27
EP2333045A3 (en) 2011-12-14
EP1869160A2 (en) 2007-12-26
EP2333045A2 (en) 2011-06-15
WO2006110677A2 (en) 2006-10-19
US8716004B2 (en) 2014-05-06
EP2333045B1 (en) 2016-11-09
EP2314667A3 (en) 2011-12-14
EP2314668A2 (en) 2011-04-27
US7691621B2 (en) 2010-04-06
DK2123746T3 (da) 2017-02-27
EP2123746A2 (en) 2009-11-25
CN101146907B (zh) 2015-04-08
US9273279B2 (en) 2016-03-01
US20140287462A1 (en) 2014-09-25
WO2006110677A3 (en) 2007-07-26
US20110070612A1 (en) 2011-03-24
EP2314668B1 (en) 2016-11-09
US20060246545A1 (en) 2006-11-02
CN101146907A (zh) 2008-03-19
DK2314667T3 (da) 2017-03-06
EP2314667B1 (en) 2016-11-23
EP2123746B1 (en) 2016-11-16
DK2333045T3 (da) 2017-02-20
EP2123746A3 (en) 2010-01-06

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