ES2912033T3 - Aptámeros y usos de los mismos - Google Patents

Aptámeros y usos de los mismos Download PDF

Info

Publication number
ES2912033T3
ES2912033T3 ES13840121T ES13840121T ES2912033T3 ES 2912033 T3 ES2912033 T3 ES 2912033T3 ES 13840121 T ES13840121 T ES 13840121T ES 13840121 T ES13840121 T ES 13840121T ES 2912033 T3 ES2912033 T3 ES 2912033T3
Authority
ES
Spain
Prior art keywords
cancer
cell
aptamers
sample
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13840121T
Other languages
English (en)
Spanish (es)
Inventor
David Halbert
Valeriy Domenyuk
David Spetzler
Tassilo Hornung
Frank Schafer
Nick Xiao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caris Science Inc
Original Assignee
Caris Science Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caris Science Inc filed Critical Caris Science Inc
Application granted granted Critical
Publication of ES2912033T3 publication Critical patent/ES2912033T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5758—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites
    • G01N33/5759—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites involving compounds localised on the membrane of tumour or cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/344—Position-specific modifications, e.g. on every purine, at the 3'-end
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/50—Determining the risk of developing a disease

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
ES13840121T 2012-10-23 2013-10-23 Aptámeros y usos de los mismos Active ES2912033T3 (es)

Applications Claiming Priority (22)

Application Number Priority Date Filing Date Title
US201261717566P 2012-10-23 2012-10-23
US201261731419P 2012-11-29 2012-11-29
US201261735915P 2012-12-11 2012-12-11
US201361748437P 2013-01-02 2013-01-02
US201361749773P 2013-01-07 2013-01-07
US201361750331P 2013-01-08 2013-01-08
US201361754471P 2013-01-18 2013-01-18
US201361767131P 2013-02-20 2013-02-20
US201361769064P 2013-02-25 2013-02-25
US201361805365P 2013-03-26 2013-03-26
US201361808144P 2013-04-03 2013-04-03
US201361820419P 2013-05-07 2013-05-07
US201361826957P 2013-05-23 2013-05-23
US201361838762P 2013-06-24 2013-06-24
US201361843256P 2013-07-05 2013-07-05
US201361862809P 2013-08-06 2013-08-06
US201361863828P 2013-08-08 2013-08-08
US201361866014P 2013-08-14 2013-08-14
US201361867978P 2013-08-20 2013-08-20
US201361871107P 2013-08-28 2013-08-28
US201361874621P 2013-09-06 2013-09-06
PCT/IB2013/003092 WO2014068408A2 (en) 2012-10-23 2013-10-23 Aptamers and uses thereof

Publications (1)

Publication Number Publication Date
ES2912033T3 true ES2912033T3 (es) 2022-05-24

Family

ID=50343809

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13840121T Active ES2912033T3 (es) 2012-10-23 2013-10-23 Aptámeros y usos de los mismos

Country Status (11)

Country Link
US (1) US9958448B2 (cg-RX-API-DMAC10.html)
EP (2) EP2912182B1 (cg-RX-API-DMAC10.html)
JP (4) JP6404822B2 (cg-RX-API-DMAC10.html)
KR (1) KR20150090072A (cg-RX-API-DMAC10.html)
CN (1) CN105452466A (cg-RX-API-DMAC10.html)
AU (3) AU2013340414B2 (cg-RX-API-DMAC10.html)
BR (1) BR112015009138A2 (cg-RX-API-DMAC10.html)
CA (1) CA2928520C (cg-RX-API-DMAC10.html)
ES (1) ES2912033T3 (cg-RX-API-DMAC10.html)
IL (2) IL238408B (cg-RX-API-DMAC10.html)
WO (1) WO2014068408A2 (cg-RX-API-DMAC10.html)

Families Citing this family (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2923757T3 (es) * 2011-12-16 2022-09-30 Modernatx Inc Composiciones de ARNm modificado
AU2013340414B2 (en) 2012-10-23 2019-05-02 Caris Science, Inc. Aptamers and uses thereof
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
AU2013361323B2 (en) 2012-12-19 2018-09-06 Caris Science, Inc. Compositions and methods for aptamer screening
WO2014144423A2 (en) * 2013-03-15 2014-09-18 Techulon Inc. Antisense molecules for treatment of staphylococcus aureus infection
WO2015083731A1 (ja) * 2013-12-04 2015-06-11 独立行政法人産業技術総合研究所 マイクロベシクルに対する核酸アプタマー
US9605266B2 (en) 2014-07-16 2017-03-28 City Of Hope Cell-specific internalizing RNA aptamers against human CCR5 and uses therefore
WO2016019270A1 (en) * 2014-07-31 2016-02-04 Academia Sinica An antagonistic pd-1 aptamer and its applications in cancer therapy related applications
US10385343B2 (en) 2014-08-29 2019-08-20 Children's Medical Center Corporation Methods and compositions for the treatment of cancer
JP6978317B2 (ja) * 2015-02-11 2021-12-08 ディーキン・ユニバーシティー ＥｐＣＡＭアプタマー及びそのコンジュゲート
US11000548B2 (en) 2015-02-18 2021-05-11 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11318163B2 (en) 2015-02-18 2022-05-03 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11304976B2 (en) 2015-02-18 2022-04-19 Enlivex Therapeutics Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11596652B2 (en) 2015-02-18 2023-03-07 Enlivex Therapeutics R&D Ltd Early apoptotic cells for use in treating sepsis
US11512289B2 (en) 2015-02-18 2022-11-29 Enlivex Therapeutics Rdo Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US11497767B2 (en) 2015-02-18 2022-11-15 Enlivex Therapeutics R&D Ltd Combination immune therapy and cytokine control therapy for cancer treatment
US10683506B2 (en) * 2015-04-10 2020-06-16 The Methodist Hospital System CD117 ligand-drug conjugates for targeted cancer therapy
US10253314B2 (en) * 2015-04-13 2019-04-09 Research Foundation Of The City University Of New York Ligand-guided-selection method for screening antigen-specific ligands
US11634704B2 (en) 2015-04-13 2023-04-25 Research Foundation Of The City University Of New York Ligand-guided-selection method for screening antigen-specific ligands
JP6803339B2 (ja) 2015-04-21 2020-12-23 エンリヴェックス セラピューティクス リミテッド 治療用のプールされた血液アポトーシス細胞調製物及びそれらの使用
WO2016176426A1 (en) 2015-04-28 2016-11-03 Duke University Thrombus imaging aptamers and methods of using same
US20180291424A1 (en) * 2015-05-20 2018-10-11 Jsr Corporation Separation method, detection method, signal measurement method, disease determination method, drug efficacy assessment method, kit, liquid composition, and specimen diluent
US10758573B2 (en) 2015-06-09 2020-09-01 President And Fellows Of Harvard College Compositions and methods for enrichment of cells
WO2017004243A1 (en) * 2015-06-29 2017-01-05 Caris Science, Inc. Therapeutic oligonucleotides
US10941176B2 (en) 2015-07-28 2021-03-09 Caris Science, Inc. Therapeutic oligonucleotides
EP3130680A1 (en) * 2015-08-11 2017-02-15 Universitat de Girona Method for the detection, follow up and/or classification of intestinal diseases
CN106018843A (zh) * 2015-11-29 2016-10-12 卢美珍 一种用于人脑疾病检测的试剂盒
DE102016100039A1 (de) * 2016-01-04 2017-07-06 Universitätsklinikum Hamburg-Eppendorf (UKE) α6-Integrin bindendes DNA-Aptamer
WO2017127762A1 (en) * 2016-01-20 2017-07-27 Vitrisa Therapeutics, Inc. Methods for improved aptamer selection
JP6884155B2 (ja) 2016-02-18 2021-06-09 エンリヴェックス セラピューティクス リミテッド 癌治療のための併用免疫療法及びサイトカイン制御療法
US11219635B2 (en) 2016-02-19 2022-01-11 City Of Hope Bi-specific aptamer
CN105755005A (zh) * 2016-03-13 2016-07-13 曹帅 一种用于骨癌检测的适配子及其试剂盒
AU2017235661B2 (en) 2016-03-18 2023-06-08 Caris Science, Inc. Oligonucleotide probes and uses thereof
EP3440463B1 (en) * 2016-04-05 2020-07-08 Université de Strasbourg Intra-droplet surface engineering to capture a molecular target
EP4134427A1 (en) * 2016-04-30 2023-02-15 Biolegend, Inc. Compositions and methods for performing magnetibuoyant separations
KR102516168B1 (ko) 2016-05-02 2023-03-30 엔코디아, 인코포레이티드 암호화 핵산을 사용한 거대분자 분석
IL298701B2 (en) 2016-05-25 2024-03-01 Caris Science Inc Oligonucleotide probes and uses thereof
EP3514237B1 (en) * 2016-09-15 2021-07-14 NEC Solution Innovators, Ltd. Secretory immunoglobulin a (siga)-binding nucleic acid molecule, sensor for siga analysis, and analysis method for siga
EP3512526A4 (en) 2016-09-16 2020-05-27 Duke University VON WILLEBRAND FACTOR (VWF) TARGETING AGENTS AND METHODS OF USE
JP2019205353A (ja) * 2016-09-27 2019-12-05 Jsr株式会社 核酸の分析方法
US10526608B2 (en) * 2016-10-31 2020-01-07 The Governors Of The University Of Alberta In vitro selection of formaldehyde cross-linking aptamers
WO2018126043A1 (en) 2016-12-30 2018-07-05 Arizona Board Of Regents On Behalf Of Arizona State University Nanoplasmonic quantification of tumor-derived extracellular vesicles in plasma microsamples
CN106636107B (zh) * 2017-01-20 2018-07-31 江苏集萃药康生物科技有限公司 一种喉癌细胞的核酸适配体及试剂盒
JP6695369B2 (ja) * 2017-02-16 2020-05-20 住友化学株式会社 硬化性樹脂組成物、硬化膜及び表示装置
JP7231229B2 (ja) 2017-03-23 2023-03-01 デューク ユニバーシティ 細胞外アプタマー染色のアンチドート媒介性解除
ES2968625T3 (es) * 2017-07-27 2024-05-13 Univ Cornell Un método para la fijación y retención de vesículas extracelulares, vesículas extracelulares fijadas y un método para diagnosticar una enfermedad
US10934549B2 (en) * 2017-08-18 2021-03-02 University Of Iowa Research Foundation Nucleic acid aptamers
WO2019079167A1 (en) 2017-10-16 2019-04-25 Institute For Cancer Research D/B/A The Research Institute Of Fox Chase Cancer Center PEPTIDES VARIANTS OF HUMAN CHORIONIC GONADOTROPIN AND TREATMENT OF BREAST CANCER
MX2020004559A (es) 2017-10-31 2020-10-05 Encodia Inc Kits para análisis utilizando codificación y/o etiqueta de ácido nucleico.
JP2021502071A (ja) * 2017-11-07 2021-01-28 エックス４ ファーマシューティカルズ， インコーポレイテッド がんバイオマーカーおよびその使用方法
JP7153460B2 (ja) * 2018-03-30 2022-10-14 シスメックス株式会社 リポタンパク質の取り込み能を測定する方法及び試薬
JP2021521273A (ja) * 2018-04-12 2021-08-26 メディアファーマ エス．アール．エル． Ｌｇａｌｓ３ｂｐ抗体−薬剤結合体及びがん治療のためのその使用
US12534734B2 (en) * 2018-05-15 2026-01-27 Drive Therapeutics Llc Stem-loop compositions and methods for inhibiting interleukin-8
EP3793715A2 (en) * 2018-05-17 2021-03-24 Meso Scale Technologies, LLC Methods for isolating surface marker displaying agents
CN108866064B (zh) * 2018-07-10 2022-05-06 中国医科大学 一种靶向转移性人乳腺癌细胞的核酸适配体及其应用
EP3827082B1 (en) * 2018-07-26 2025-05-14 Bracco Imaging SpA Icam-1 aptamers, diagnostic and therapeutic uses thereof
KR102893528B1 (ko) * 2018-07-27 2025-11-28 베라바스, 인크. 고갈 및 농축을 위한 방법
WO2020033585A1 (en) * 2018-08-07 2020-02-13 The Broad Institute, Inc. Methods for combinatorial screening and use of therapeutic targets thereof
US11752175B2 (en) 2018-09-21 2023-09-12 Cornell University Compositions and methods for glaucoma
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
US12631624B2 (en) 2018-10-23 2026-05-19 Meso Scale Technologies, Llc. Methods for isolating surface marker displaying agents
EP3870969A4 (en) * 2018-10-24 2022-07-27 Path Ex, Inc. PROCESS FOR RECOVERING AND ISOLATION OF DISEASE MATERIAL FROM FLOWING MATERIAL
EP3887826A1 (en) 2018-11-30 2021-10-06 Corning Incorporated System and method for analyzing extracellular vesicles with an optical biosensor
US11766484B2 (en) * 2019-01-03 2023-09-26 International Business Machines Corporation Exosome vessels for delivery of molecular cargo
CN109580960A (zh) * 2019-01-14 2019-04-05 周明 细胞外囊泡的分离方法、以及检测细胞外囊泡表面标志物的方法和试剂盒
CN111487222B (zh) * 2019-01-28 2023-11-14 上海乐合生物科技有限公司 一种检测bnp的spr芯片的制备方法
JP7670336B2 (ja) * 2019-03-07 2025-04-30 ユニバーシティ オブ マイアミ Ｒｎａアプタマー及びその使用
JP7740988B2 (ja) * 2019-03-12 2025-09-17 バイオエヌテック エスエー 前立腺癌のための治療用ｒｎａ
KR102212149B1 (ko) * 2019-03-28 2021-02-04 부산대학교 산학협력단 암 줄기세포에 특이적으로 결합하는 압타머 및 이의 용도
EP3963070A4 (en) 2019-04-30 2023-02-22 Encodia, Inc. METHODS OF PREPARATION OF ANALYTES AND RELATED KITS
CA3142278A1 (en) * 2019-06-05 2020-12-10 Liv Process, Inc. Aptamers against clostridium difficile
CN112083168A (zh) * 2019-06-14 2020-12-15 复旦大学附属华山医院 多肽段诊断模型及其在预测髓母细胞瘤转移风险中的应用
WO2020257401A1 (en) 2019-06-21 2020-12-24 The Children's Medical Center Corporation Methods and compositions for the treatment of cancer
CN111235106B (zh) * 2019-08-30 2022-08-05 武汉大学 一种靶向肿瘤细胞的Aptamer-CD3+T细胞及其构建方法与应用
CN110522724A (zh) * 2019-09-10 2019-12-03 华东理工大学 包载基因药物的靶向EpCAM脂质体纳米颗粒及制备方法、应用
WO2021051011A1 (en) * 2019-09-13 2021-03-18 Google Llc Methods and compositions for protein and peptide sequencing
IT201900018239A1 (it) * 2019-10-08 2021-04-08 Consiglio Nazionale Ricerche Metodo per l'isolamento di vescicole extracellulari intatte
WO2021102919A1 (zh) * 2019-11-29 2021-06-03 京东方科技集团股份有限公司 类肽化合物以及表面偶联有类肽化合物的检测芯片
CN110982821B (zh) * 2019-12-27 2023-07-07 广东石油化工学院 一种具有黄嘌呤氧化酶抑制活性的核酸适配体及其应用
EP3868884A1 (en) * 2020-02-19 2021-08-25 Aarhus Universitet Method for subtyping bladder cancer using aptamers
WO2021178209A1 (en) * 2020-03-03 2021-09-10 Aptitude Medical Systems, Inc. Aptamers and use thereof
CN111518883B (zh) * 2020-04-02 2022-12-30 深圳大学 一种用于冠心病诊断的血浆miRNA标志物及其应用
CN111574617A (zh) * 2020-04-26 2020-08-25 无锡可尚生物科技有限公司 一种防治老年人骨质疏松的胶原蛋白肽及其制备方法
CN111579785B (zh) * 2020-04-29 2022-09-13 南方医科大学深圳医院 一种基于血浆外泌体蛋白的hpv感染致宫颈癌的早期诊断标志物及其应用
CN111549034B (zh) * 2020-05-21 2022-11-04 昆明理工大学 与趋化因子配体-5特异性结合的核酸适配体及其应用
CN111999270A (zh) * 2020-06-10 2020-11-27 北京工业大学 一种基于回音壁模式光学微腔的外泌体检测方法
CN113817740B (zh) * 2020-06-19 2024-07-09 中国科学院化学研究所 一种核酸适配体识别并结合ptprf及其相关功能的应用研究
MX2022016049A (es) 2020-06-25 2023-02-09 Procter & Gamble Aptameros para aplicaciones para el cuidado personal de la salud.
KR20230044429A (ko) * 2020-07-08 2023-04-04 메르시 바이오애널리틱스, 인코포레이티드 폐암의 검출을 위한 조성물 및 방법
WO2022023564A1 (en) * 2020-07-30 2022-02-03 Assistance Publique - Hopitaux De Paris Whole nucleic acids rt-qpcr method for detecting histoplasma capsulatum fungus pathogen(s), histoplasmosis diagnosis and treatment methods, means thereof
US20230340599A1 (en) * 2020-09-22 2023-10-26 The Secretary Of State For Defence Apparatus, kits and methods for predicting the development of sepsis
US11664189B2 (en) * 2020-10-04 2023-05-30 Borries Pte. Ltd. Apparatus of charged-particle beam such as scanning electron microscope comprising plasma generator, and method thereof
CA3196924A1 (en) * 2020-10-05 2022-04-14 Gina-Life Diagnostics Ltd. Identification and characterization of ovarian cancer specific biomarkers in vaginal secretions
EP4255467A1 (en) * 2020-12-07 2023-10-11 Institute For Cancer Research d/b/a The Research Institute of Fox Chase Cancer Center Treatment of females having brca1/2 mutations with human chorionic gonadotropin to reduce the risk of developing breast cancer
CA3183773A1 (en) * 2020-12-21 2022-06-30 Cassandra CHAMOUN Selecting aptamers using sequencing
CN116710781A (zh) * 2021-01-05 2023-09-05 密歇根大学董事会 用于抗体表征的方式
WO2022148777A1 (en) * 2021-01-05 2022-07-14 The Regents Of The University Of Michigan Means for antibody characterization
US12631636B2 (en) * 2021-02-04 2026-05-19 Fresenius Medical Care Holdings, Inc. Peptidoglycan (PGN) aptamers and associated methods
CN113456797B (zh) * 2021-06-18 2024-03-08 暨南大学 长春碱类衍生物在制备治疗骨肉瘤和/或软组织肉瘤药物中的应用
CN113481205B (zh) * 2021-07-30 2023-11-03 杭州凡泰生物科技有限公司 一种hpv18类病毒颗粒的核酸适配体hpv1801及其应用
BR102021018527A2 (pt) * 2021-09-17 2023-03-28 Fundação Oswaldo Cruz Aptâmero de ácido nucleico, composição, uso de um aptâmero, kit diagnóstico, método para detectar ou diagnosticar um tumor, e, método para tratamento de câncer
CN113884685B (zh) * 2021-10-18 2023-03-14 中国农业大学 牛结核病血清学诊断标志物及其临床应用
JPWO2023100486A1 (cg-RX-API-DMAC10.html) 2021-12-02 2023-06-08
WO2023100567A1 (ja) 2021-12-02 2023-06-08 株式会社デンソー 分析方法
CN114323863B (zh) * 2021-12-31 2023-12-22 吉林大学 一种外泌体阵列捕获探针的制备方法与应用
CN114460310A (zh) * 2022-04-12 2022-05-10 天津康博尔生物基因技术有限公司 一种彩色乳胶微球及其制备方法与应用
CN117229363A (zh) * 2022-06-06 2023-12-15 天津赛德生物制药有限公司 用于肺癌早期诊断的肽核酸功能化核孔膜及制备方法与应用
CN119343465A (zh) * 2022-07-05 2025-01-21 卫材R&D管理有限公司 生物流体配体文库富集技术及其用途
US20260139318A1 (en) * 2022-11-17 2026-05-21 Universite De Poitiers Method for determining microsatellite instability status, kits and uses thereof
CN115806993B (zh) * 2022-12-27 2023-11-14 中国人民解放军军事科学院军事医学研究院 特异识别人绒毛促性腺激素的核酸适配体hcg-2
CN115747221B (zh) * 2022-12-27 2023-11-21 中国人民解放军军事科学院军事医学研究院 Hcg-52及其在特异识别人绒毛促性腺激素中的应用
CN116286831B (zh) * 2023-01-29 2024-02-20 珠海市人民医院 靶向egfr t790m突变体的核酸适配体及其在制备治疗肺癌药物中的应用
CN116272708B (zh) * 2023-03-16 2023-11-14 海南医学院 一种量子点-抗体复合物微球及其制备方法、应用
WO2024215162A1 (ko) * 2023-04-13 2024-10-17 (주)메디엔진 단일 융합 단백질 및 이를 포함하는 약학적 조성물
CN118409092B (zh) * 2023-09-21 2024-10-18 上海市肺科医院（上海市职业病防治院） 一种用于干扰素γ检测的核酸适体功能化磁珠及其制备方法和试剂盒
CN117783055B (zh) * 2023-12-26 2024-10-01 西南大学 一种基于亲和释放的脂质体修饰物的筛选方法
WO2025197978A1 (ja) * 2024-03-19 2025-09-25 合同会社Ｈ．Ｕ．グループ中央研究所 核酸の回収方法
WO2025205534A1 (ja) * 2024-03-25 2025-10-02 積水メディカル株式会社 免疫測定方法、精度管理方法、標準試料及び免疫測定試薬
CN118460549B (zh) * 2024-07-12 2024-09-24 上海交通大学医学院附属仁济医院 特异性识别cd276的核酸适体及其应用
KR20260055294A (ko) * 2024-10-15 2026-04-23 재단법인대구경북과학기술원 혈액-뇌 장벽 투과용 약물전달체 및 이를 포함하는 뇌 질환 치료 또는 진단용 조성물
CN119500128B (zh) * 2024-12-03 2025-09-16 吉林大学 Dna驱动纳米酶/生物酶复合材料制备及速灭威检测应用
CN119804853A (zh) * 2024-12-30 2025-04-11 北京中生金域诊断技术股份有限公司 一种前白蛋白免疫层析检测试剂条的制备方法及应用

Family Cites Families (229)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US794764A (en) 1905-02-13 1905-07-18 Peter M Wolf Pump-platform.
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4551435A (en) 1983-08-24 1985-11-05 Immunicon, Inc. Selective removal of immunospecifically recognizable substances from solution
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
US4925788A (en) 1986-10-24 1990-05-15 Immunicon Corporation Immunoassay system and procedure based on precipitin-like interaction between immune complex and Clq or other non-immunospecific factor
US5158871A (en) 1988-02-12 1992-10-27 University Of Connecticut Method of using magnetic particles for isolating, collecting and assaying diagnostic ligates
US5108933A (en) 1988-09-16 1992-04-28 Immunicon Corporation Manipulation of colloids for facilitating magnetic separations
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US6309822B1 (en) 1989-06-07 2001-10-30 Affymetrix, Inc. Method for comparing copy number of nucleic acid sequences
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
SE470347B (sv) 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Mikrostruktur för vätskeflödessystem och förfarande för tillverkning av ett sådant system
US5496938A (en) 1990-06-11 1996-03-05 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands to HIV-RT and HIV-1 rev
US5853984A (en) 1990-06-11 1998-12-29 Nexstar Pharmaceuticals, Inc. Use of nucleic acid ligands in flow cytometry
US5705337A (en) 1990-06-11 1998-01-06 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chemi-SELEX
US5707796A (en) 1990-06-11 1998-01-13 Nexstar Pharmaceuticals, Inc. Method for selecting nucleic acids on the basis of structure
US5789163A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Enzyme linked oligonucleotide assays (ELONAS)
US5683867A (en) 1990-06-11 1997-11-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: blended SELEX
US5789157A (en) 1990-06-11 1998-08-04 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5650275A (en) 1990-06-11 1997-07-22 Nexstar Pharmacueticals Inc Target detection method using spectroscopically detectable nucleic acid ligands
US5763177A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
US5567588A (en) 1990-06-11 1996-10-22 University Research Corporation Systematic evolution of ligands by exponential enrichment: Solution SELEX
US5864026A (en) 1990-06-11 1999-01-26 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5580737A (en) 1990-06-11 1996-12-03 Nexstar Pharmaceuticals, Inc. High-affinity nucleic acid ligands that discriminate between theophylline and caffeine
ATE318832T1 (de) 1990-06-11 2006-03-15 Gilead Sciences Inc Verfahren zur vervendung von nukleinsäureliganden
US5763566A (en) 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
US6716580B2 (en) 1990-06-11 2004-04-06 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US6569620B1 (en) 1990-06-11 2003-05-27 Somalogic, Inc. Method for the automated generation of nucleic acid ligands
US5637459A (en) 1990-06-11 1997-06-10 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: chimeric selex
US5756287A (en) 1990-06-11 1998-05-26 Nexstar Pharmaceuticals, Inc. High affinity HIV integrase inhibitors
US6011020A (en) 1990-06-11 2000-01-04 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand complexes
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
US5660985A (en) 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
US5712375A (en) 1990-06-11 1998-01-27 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5861254A (en) 1997-01-31 1999-01-19 Nexstar Pharmaceuticals, Inc. Flow cell SELEX
US5648214A (en) 1990-06-11 1997-07-15 University Research Corporation High-affinity oligonucleotide ligands to the tachykinin substance P
US5200084A (en) 1990-09-26 1993-04-06 Immunicon Corporation Apparatus and methods for magnetic separation
AU649074B2 (en) 1990-10-12 1994-05-12 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Modified ribozymes
IE920151A1 (en) 1991-01-18 1992-07-29 Univ New York A novel expression-cloning method for identifying target¹proteins for receptor tyrosine kinases and novel human grb¹proteins
US5186827A (en) 1991-03-25 1993-02-16 Immunicon Corporation Apparatus for magnetic separation featuring external magnetic means
US6864048B2 (en) 1993-04-28 2005-03-08 Affymetrix, Inc. Factorial chemical libraries
US5817635A (en) 1993-08-09 1998-10-06 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Modified ribozymes
US6013443A (en) 1995-05-03 2000-01-11 Nexstar Pharmaceuticals, Inc. Systematic evolution of ligands by exponential enrichment: tissue SELEX
EP0823914B1 (en) 1995-05-03 2004-08-25 Gilead Sciences, Inc. Systematic evolution of ligands by exponential enrichment: tissue selex
US5736330A (en) 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
DE69735292T2 (de) 1996-10-25 2006-10-19 Gilead Sciences, Inc., Foster City Gefaesszellen wachstumsfaktor (vegf) nukleinsäureligand-komplexe
US6426335B1 (en) 1997-10-17 2002-07-30 Gilead Sciences, Inc. Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
US6051698A (en) 1997-06-06 2000-04-18 Janjic; Nebojsa Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
EP0996455B1 (en) 1997-04-16 2009-12-16 Sloan-Kettering Institute For Cancer Research Alpha-o-linked glycoconjugates with clustered (2,6)-st epitopes, methods of preparation and uses thereof
AU8505398A (en) 1997-07-23 1999-02-16 Northeastern University Methods for enhancing or reducing preimplantation embryo survival rates
US6632526B1 (en) 1997-10-14 2003-10-14 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
US20060057573A1 (en) 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
US20070166741A1 (en) 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US6719868B1 (en) 1998-03-23 2004-04-13 President And Fellows Of Harvard College Methods for fabricating microfluidic structures
US6180348B1 (en) 1998-04-20 2001-01-30 Weihua Li Method of isolating target specific oligonucleotide ligands
US6986902B1 (en) 1998-04-28 2006-01-17 Inex Pharmaceuticals Corporation Polyanionic polymers which enhance fusogenicity
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6867289B1 (en) 1998-10-26 2005-03-15 Board Of Regents, The University Of Texas Systems Thio-modified aptamer synthetic methods and compositions
US6623526B1 (en) 1999-01-08 2003-09-23 Corin Limited Knee prosthesis
US6329145B1 (en) 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6379698B1 (en) 1999-04-06 2002-04-30 Isis Pharmaceuticals, Inc. Fusogenic lipids and vesicles
US7074586B1 (en) 1999-06-17 2006-07-11 Source Precision Medicine, Inc. Quantitative assay for low abundance molecules
US6811668B1 (en) 1999-06-22 2004-11-02 Caliper Life Sciences, Inc. Apparatus for the operation of a microfluidic device
US6929030B2 (en) 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
CN100402850C (zh) 1999-06-28 2008-07-16 加利福尼亚技术学院 微型制造的弹性体的阀和泵系统
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7195670B2 (en) 2000-06-27 2007-03-27 California Institute Of Technology High throughput screening of crystallization of materials
AUPQ147799A0 (en) 1999-07-07 1999-07-29 Medvet Science Pty. Ltd. Mesenchymal precursor cell
US6387620B1 (en) 1999-07-28 2002-05-14 Gilead Sciences, Inc. Transcription-free selex
US6506887B1 (en) 1999-07-29 2003-01-14 Somalogic, Incorporated Conditional-selex
WO2001051918A1 (en) 2000-01-12 2001-07-19 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
US7452713B2 (en) 2000-02-29 2008-11-18 Stmicroelectronics S.R.L. Process for manufacturing a microfluidic device with buried channels
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
WO2001078087A2 (en) 2000-04-06 2001-10-18 Luminex Corporation Magnetically-responsive microspheres
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
WO2002016904A2 (en) 2000-08-23 2002-02-28 Imego Ab Microfluidic device and method with transport of magnetic particles into and out of wells
US20020048534A1 (en) 2000-08-24 2002-04-25 David Storek Sample preparing arrangement and a method relating to such an arrangement
CA2418724A1 (en) 2000-09-13 2002-03-21 Archemix Corporation Target activated nucleic acid biosensor and methods of using same
US6376190B1 (en) 2000-09-22 2002-04-23 Somalogic, Inc. Modified SELEX processes without purified protein
WO2002026932A2 (en) 2000-09-26 2002-04-04 Duke University Rna aptamers and methods for identifying the same
EP1409720A4 (en) 2000-10-27 2005-09-07 Res Dev Foundation IN VITRO SELECTION OF SIGNAL PATTERNS
US7083958B2 (en) 2000-11-20 2006-08-01 The Board Of Trustees Of The University Of Illinois Membrane scaffold proteins
US6822454B2 (en) 2000-12-01 2004-11-23 Protasis Corporation Microfluidic device with multiple microcoil NMR detectors and field gradient focusing
US20020097419A1 (en) 2001-01-19 2002-07-25 Chang William Ho Information apparatus for universal data output
DE10109897A1 (de) 2001-02-21 2002-11-07 Novosom Ag Fakultativ kationische Liposomen und Verwendung dieser
DE10109898A1 (de) 2001-02-21 2002-09-05 Novosom Gmbh Lipide mit veränderlicher Ladung
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US6802342B2 (en) 2001-04-06 2004-10-12 Fluidigm Corporation Microfabricated fluidic circuit elements and applications
WO2002099077A2 (en) 2001-06-06 2002-12-12 Proteologics, Inc. Methods and compositions related to tagging of membrane surface proteins
US7390463B2 (en) 2001-09-07 2008-06-24 Corning Incorporated Microcolumn-based, high-throughput microfluidic device
DE60237289D1 (de) 2001-09-17 2010-09-23 Gyros Patent Ab Einen kontrollierten strom in einer mikrofluidvorrichtung ermöglichende funktionseinheit
DE60233885D1 (de) 2001-10-16 2009-11-12 Kode Biotech Ltd Aus modifizierten zellen präparierte empfindlichkeitskontrollen für blutserologie
US7253003B2 (en) 2001-10-19 2007-08-07 Wisconsin Alumni Research Foundation Method for monitoring the environment within a microfluidic device
US7189580B2 (en) 2001-10-19 2007-03-13 Wisconsin Alumni Research Foundation Method of pumping fluid through a microfluidic device
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
EP1463796B1 (en) 2001-11-30 2013-01-09 Fluidigm Corporation Microfluidic device and methods of using same
AU2002360499A1 (en) 2001-12-05 2003-06-17 University Of Washington Microfluidic device and surface decoration process for solid phase affinity binding assays
US7238255B2 (en) 2001-12-31 2007-07-03 Gyros Patent Ab Microfluidic device and its manufacture
US7858117B2 (en) 2002-02-21 2010-12-28 Novosom Ag Amphoteric liposomes and their use
US6958119B2 (en) 2002-02-26 2005-10-25 Agilent Technologies, Inc. Mobile phase gradient generation microfluidic device
US20030219801A1 (en) 2002-03-06 2003-11-27 Affymetrix, Inc. Aptamer base technique for ligand identification
US7195986B1 (en) 2002-03-08 2007-03-27 Caliper Life Sciences, Inc. Microfluidic device with controlled substrate conductivity
NZ518163A (en) 2002-04-05 2005-04-29 Kiwi Ingenuity Ltd Embryo modified with a glycolipid to enhance implantation into the endometrium
AU2002253468B2 (en) 2002-04-18 2008-04-03 Esbatech Ag Method for the identification of modulators of a secretase activity
GB2388189B (en) 2002-04-29 2006-01-11 Robert Jeffrey Geddes Carr Optical detection and analysis of particles
US7189581B2 (en) 2002-04-30 2007-03-13 Wisconsin Alumni Research Foundation Method of obtaining a sample concentration of a solution in a microfluidic device
EP1362827B1 (en) 2002-05-16 2008-09-03 Micronit Microfluidics B.V. Method of fabrication of a microfluidic device
US7288368B2 (en) 2002-06-17 2007-10-30 Stephen Eliot Zweig Membrane receptor reagent and assay
WO2004007582A2 (en) 2002-07-15 2004-01-22 University Of Virginia Patent Foundation Hybrid polymers for functional tuning of microfluidic device surfaces
EP1552302B1 (en) 2002-07-24 2009-03-25 Bio-Rad Laboratories, Inc. Protein interaction difference mapping
US7452509B2 (en) 2002-07-26 2008-11-18 Applied Biosystems Inc. Microfluidic device including displaceable material trap, and system
US7201881B2 (en) 2002-07-26 2007-04-10 Applera Corporation Actuator for deformable valves in a microfluidic device, and method
US7135147B2 (en) 2002-07-26 2006-11-14 Applera Corporation Closing blade for deformable valve in a microfluidic device and method
KR100480338B1 (ko) 2002-08-08 2005-03-30 한국전자통신연구원 극소량의 유체제어를 위한 미세 유체제어소자
TW536524B (en) 2002-09-17 2003-06-11 Fan-Gen Tzeng Network-type micro-channel device for micro-fluid
US7118661B2 (en) 2002-09-30 2006-10-10 The Regents Of The University Of California Nanolaminate microfluidic device for mobility selection of particles
EP2719756B1 (en) 2002-10-04 2017-04-05 The Regents of the University of California Microfluidic multi-compartment device for neuroscience research
CA2502610A1 (en) 2002-10-16 2005-01-13 Board Of Regents Of The University Of Texas System Bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries
US20050037394A1 (en) 2002-12-03 2005-02-17 Keefe Anthony D. Method for in vitro selection of 2'-substituted nucleic acids
WO2004050899A2 (en) 2002-12-03 2004-06-17 Archemix Corporation Method for in vitro selection of 2’-substituted nucleic acids
FR2848125B1 (fr) 2002-12-04 2006-06-09 Commissariat Energie Atomique Dispositif microfluidique dans lequel l'interface liquide/fluide est stabilisee
US7467928B2 (en) 2002-12-12 2008-12-23 Board Of Trustees Of The University Of Arkansas Microfluidic device utilizing magnetohydrodynamics and method for fabrication thereof
US7125711B2 (en) 2002-12-19 2006-10-24 Bayer Healthcare Llc Method and apparatus for splitting of specimens into multiple channels of a microfluidic device
US7338637B2 (en) 2003-01-31 2008-03-04 Hewlett-Packard Development Company, L.P. Microfluidic device with thin-film electronic devices
US7413709B2 (en) 2003-02-12 2008-08-19 Agilent Technologies, Inc. PAEK-based microfluidic device with integrated electrospray emitter
JP3856763B2 (ja) 2003-03-11 2006-12-13 財団法人川村理化学研究所 マイクロ流体素子の製造方法
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
EP1615721B1 (en) 2003-04-03 2014-06-18 Fluidigm Corporation Microfluidic devices and methods of using same
US7422725B2 (en) 2003-05-01 2008-09-09 Enplas Corporation Sample handling unit applicable to microchip, and microfluidic device having microchips
US7544506B2 (en) 2003-06-06 2009-06-09 Micronics, Inc. System and method for heating, cooling and heat cycling on microfluidic device
WO2005024042A2 (en) 2003-09-04 2005-03-17 The Regents Of The University Of California Aptamers and methods for their in vitro selection and uses thereof
FR2862007B1 (fr) 2003-11-12 2005-12-23 Commissariat Energie Atomique Dispositif microfluidique muni d'un nez d'electronebulisation.
US7807351B2 (en) 2003-11-22 2010-10-05 Techno Medica Co., Ltd. Method of detecting target molecule by using aptamer
US7329391B2 (en) 2003-12-08 2008-02-12 Applera Corporation Microfluidic device and material manipulating method using same
EP1547688A1 (en) 2003-12-23 2005-06-29 STMicroelectronics S.r.l. Microfluidic device and method of locally concentrating electrically charged substances in a microfluidic device
US7099778B2 (en) 2003-12-30 2006-08-29 Caliper Life Sciences, Inc. Method for determining diffusivity and molecular weight in a microfluidic device
US7611863B2 (en) 2004-01-08 2009-11-03 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Selective staining of biomembranes using voltage-sensitive dyes
US7351380B2 (en) 2004-01-08 2008-04-01 Sandia Corporation Microfluidic structures and methods for integrating a functional component into a microfluidic device
US20080261204A1 (en) 2004-01-23 2008-10-23 Lingvitae As Polynucleotide Ligation Reactions
CA2554240A1 (en) 2004-01-25 2005-08-11 Fluidigm Corporation Crystal forming devices and systems and methods for making and using the same
AU2005223715A1 (en) 2004-03-22 2005-09-29 Kode Biotech Limited Synthetic membrane anchors
US7402229B2 (en) 2004-03-31 2008-07-22 Intel Corporation Fabrication and use of semipermeable membranes and gels for the control of electrolysis in a microfluidic device
US7575722B2 (en) 2004-04-02 2009-08-18 Eksigent Technologies, Inc. Microfluidic device
US7419639B2 (en) 2004-05-12 2008-09-02 The Board Of Trustees Of The Leland Stanford Junior University Multilayer microfluidic device
WO2005121864A2 (en) 2004-06-07 2005-12-22 Fluidigm Corporation Optical lens system and method for microfluidic devices
US7514400B2 (en) 2004-10-06 2009-04-07 The Penn State Research Foundation Synthetic mimics of mammalian cell surface receptors: method and compositions
JP2008523354A (ja) 2004-11-17 2008-07-03 ルミネックス・コーポレーション フロー・アナライザからデータを管理するための装置と方法
US7488596B2 (en) 2004-12-17 2009-02-10 Samsung Electronics Co., Ltd. Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same
EP2857523A1 (en) 2005-02-01 2015-04-08 Applied Biosystems, LLC Method for identifying a sequence in a polynucleotide
CN101663576B (zh) 2005-04-12 2012-05-23 卡钳生命科学股份有限公司 用于微流体器件的紧凑型光检测系统
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
KR100590581B1 (ko) 2005-05-10 2006-06-19 삼성전자주식회사 미세유동장치 및 그 제조방법
CA2609328A1 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
JP4992201B2 (ja) 2005-06-07 2012-08-08 富士ゼロックス株式会社 マイクロ流体制御方法、マイクロ流体素子およびその製造方法
JP4706019B2 (ja) 2005-07-08 2011-06-22 国立大学法人東京農工大学 アプタマーの同定方法
US20100254901A1 (en) 2005-07-28 2010-10-07 Smith Cassandra L Compositions comprising nucleic acid aptamers
WO2007032359A1 (ja) 2005-09-12 2007-03-22 National University Corporation Tokyo University Of Agriculture And Technology アプタマー・プローブ複合体を用いた標的分子の検出方法
US8367627B2 (en) 2005-09-15 2013-02-05 Duke University Focused libraries, functional profiling, laser SELEX, and DESELEX
TWI274040B (en) 2005-12-23 2007-02-21 Ind Tech Res Inst Microfluidic device and method of manufacturing the same
US7568399B2 (en) 2006-01-05 2009-08-04 Integrated Sensing Systems, Inc. Microfluidic device
US7581429B2 (en) 2006-01-06 2009-09-01 Integrated Sensing Systems, Inc. Microfluidic device and method of operation
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
US8492084B2 (en) 2006-01-24 2013-07-23 Koji Sode Method and apparatus for assaying test substance in sample
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
AU2007237909A1 (en) 2006-04-19 2007-10-25 Applied Biosystems, Llc. Reagents, methods, and libraries for gel-free bead-based sequencing
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
US8048448B2 (en) 2006-06-15 2011-11-01 Abbott Cardiovascular Systems Inc. Nanoshells for drug delivery
WO2008038696A1 (en) 2006-09-27 2008-04-03 National University Corporation Tokyo University Of Agriculture And Technology Method of assaying target substance in sample, aptamer molecule and method of constructing the same
EP2099930B1 (en) 2006-12-13 2015-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
CN101536072B (zh) 2006-12-15 2012-07-11 欧司朗股份有限公司 带有专用的色彩调节的发光二极管模块以及相应的方法
US7947447B2 (en) 2007-01-16 2011-05-24 Somalogic, Inc. Method for generating aptamers with improved off-rates
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
ES3045958T3 (en) 2007-07-17 2025-12-01 Somalogic Operating Co Inc Method for generating aptamers with improved off-rates
GB2459228B (en) * 2007-07-25 2012-06-20 Univ Louisville Res Found Exosome-associated microrna as a diagnostic marker
US8431367B2 (en) 2007-09-14 2013-04-30 Predictive Biosciences Corporation Detection of nucleic acids and proteins
BRPI0816852A2 (pt) 2007-09-14 2017-06-06 Univ Ohio State Res Found método de diagnosticar ou prognosticar doença ou desordem em sujeito, biomarcador, método para determinar e/ou prever se sujeito tem diferenciação da desordem, biomarcador para desordem pulmonar, método para diagnosticar se sujeito tem, ou está em risco de desenvolver doença ou desordem, método de inibir a proliferação de doença ou desordem e método para identificar agente terapêutico do câncer.
US8975215B2 (en) 2007-09-17 2015-03-10 Postech Foundation Methods for producing surface bound oligonucleotide on solid substrate and uses thereof
ES2448426T3 (es) 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
US8088601B2 (en) 2007-11-15 2012-01-03 Wisconsin Alumni Research Foundation Expression systems for functional membrane polypeptides
US8008019B2 (en) 2007-11-28 2011-08-30 Luminex Molecular Diagnostics Use of dual-tags for the evaluation of genomic variable repeat regions
AU2009212543B2 (en) * 2008-02-01 2015-07-09 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
US9193968B2 (en) 2008-03-12 2015-11-24 Aptamatrix, Inc. Library compositions and methods for acyclic identification of aptamers
CN102066562A (zh) 2008-03-12 2011-05-18 雪城大学 结构限定的适配子的直接选择
EP2159286A1 (en) 2008-09-01 2010-03-03 Consiglio Nazionale Delle Ricerche Method for obtaining oligonucleotide aptamers and uses thereof
US10359425B2 (en) 2008-09-09 2019-07-23 Somalogic, Inc. Lung cancer biomarkers and uses thereof
US20100221752A2 (en) 2008-10-06 2010-09-02 Somalogic, Inc. Ovarian Cancer Biomarkers and Uses Thereof
JP2012507300A (ja) 2008-10-30 2012-03-29 カリス ライフ サイエンシズ ルクセンブルク ホールディングス Ｒｎａパターンを評価する方法
EP2350320A4 (en) * 2008-11-12 2012-11-14 Caris Life Sciences Luxembourg Holdings METHOD AND SYSTEMS FOR USE OF EXOSOMES FOR THE DETERMINATION OF PHENOTYPES
EP2202522A1 (en) 2008-12-23 2010-06-30 Universiteit Leiden Methods for immobilizing microvesicles, means and methods for detecting them, and uses thereof
EP2391343B1 (en) 2009-01-29 2017-03-01 Arbutus Biopharma Corporation Improved lipid formulation for the delivery of nucleic acids
US8633140B2 (en) 2009-02-27 2014-01-21 The Regents Of The University Of Michigan Functionalized polydiacetylene sensors
NZ597504A (en) 2009-06-15 2013-10-25 Alnylam Pharmaceuticals Inc Lipid formulated dsrna targeting the pcsk9 gene
US20130029339A1 (en) 2009-09-09 2013-01-31 The General Hospital Corporation Use of microvesicles in analyzing kras mutations
US20120264810A1 (en) 2009-09-22 2012-10-18 The University Of British Columbia Compositions and methods for enhancing cellular uptake and intracellular delivery of lipid particles
CA2779223A1 (en) 2009-10-27 2011-05-12 Caris Mpi, Inc. Molecular profiling for personalized medicine
CA2782284A1 (en) 2009-11-30 2011-06-03 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Methods and systems for isolating, storing, and analyzing vesicles
EP2524059A4 (en) 2010-01-13 2013-11-20 Caris Life Sciences Luxembourg Holdings DETECTION OF GASTROINTESTINAL DISEASES
JP2011193873A (ja) 2010-02-26 2011-10-06 Canon Inc 核酸リガンドのスクリーニング方法
AU2011223789A1 (en) * 2010-03-01 2012-09-20 Caris Life Sciences Switzerland Holdings Gmbh Biomarkers for theranostics
KR20130043104A (ko) 2010-04-06 2013-04-29 카리스 라이프 사이언스 룩셈부르크 홀딩스 질병용 순환 생물학적 지표들
NZ602618A (en) 2010-04-12 2013-12-20 Somalogic Inc Aptamers to beta-ngf and their use in treating beta-ngf mediated diseases and disorders
EP4481047A3 (en) 2010-06-03 2025-04-16 Alnylam Pharmaceuticals, Inc. Biodegradable lipids for the delivery of active agents
US20130116129A1 (en) 2010-07-01 2013-05-09 National Institute Of Advanced Industrial Science And Technology Method for detecting target molecules
JP5905003B2 (ja) 2010-07-09 2016-04-20 ソマロジック・インコーポレーテッド 肺癌バイオマーカーとその使用
WO2012024543A1 (en) * 2010-08-18 2012-02-23 Caris Life Sciences Luxembourg Holdings Circulating biomarkers for disease
ES2688048T3 (es) 2010-09-27 2018-10-30 Somalogic, Inc. Biomarcadores del mesotelioma y usos de los mismos
AU2011352167A1 (en) 2010-12-28 2013-07-11 Caris Mpi, Inc. Molecular profiling for cancer
JP2014507160A (ja) * 2011-02-22 2014-03-27 カリス ライフ サイエンシズ ルクセンブルク ホールディングス エス．アー．エール．エル． 循環バイオマーカー
JP2014526032A (ja) 2011-06-07 2014-10-02 カリス ライフ サイエンシズ ルクセンブルク ホールディングス エス．アー．エール．エル． 癌に対する循環バイオマーカー
EP2721179A4 (en) 2011-06-16 2014-10-01 Caris Life Sciences Luxembourg Holdings S A R L BIOMARKER COMPOSITIONS AND METHOD THEREFOR
CA2865335A1 (en) 2012-03-09 2013-09-12 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Biomarker compositions and methods
US9119701B2 (en) 2012-10-22 2015-09-01 Alcon Research, Ltd. Pressure control in phacoemulsification system
AU2013340414B2 (en) 2012-10-23 2019-05-02 Caris Science, Inc. Aptamers and uses thereof
CA2892490A1 (en) 2012-11-26 2014-05-30 Caris Science, Inc. Biomarker compositions and methods
BR112016004153A2 (pt) 2013-08-28 2019-09-17 Caris Life Sciences Switzerland Holdings Gmbh "método para caracterizar uma doença ou distúrbio, kit, composição, método para gerar uma biblioteca de entrada, oligonucleotídeo, pluralidade de oligonucleotídeos e método para identificar um aptâmero
JP2016031772A (ja) 2014-07-30 2016-03-07 株式会社日立ハイテクファインシステムズ 熱アシスト磁気ヘッド素子の検査方法及びその装置
US9815124B1 (en) 2016-03-22 2017-11-14 Flamur Tulovic Hole saw with threadably removable portion

Also Published As

Publication number Publication date
IL269045B (en) 2021-03-25
IL238408B (en) 2019-09-26
JP2016506716A (ja) 2016-03-07
AU2013340414A1 (en) 2015-05-14
JP2019033750A (ja) 2019-03-07
JP6933619B2 (ja) 2021-09-08
EP4170031A1 (en) 2023-04-26
JP7442483B2 (ja) 2024-03-04
US20160003835A1 (en) 2016-01-07
IL238408A0 (en) 2015-06-30
WO2014068408A3 (en) 2014-11-27
EP2912182A2 (en) 2015-09-02
CA2928520C (en) 2023-03-14
EP2912182B1 (en) 2021-12-08
JP2022017220A (ja) 2022-01-25
AU2021269417B2 (en) 2024-09-19
CN105452466A (zh) 2016-03-30
AU2019208237A1 (en) 2019-08-15
IL269045A (en) 2019-10-31
JP2024059757A (ja) 2024-05-01
WO2014068408A2 (en) 2014-05-08
AU2013340414B2 (en) 2019-05-02
AU2019208237B2 (en) 2021-12-02
EP4170031B1 (en) 2026-07-29
CA2928520A1 (en) 2014-05-08
AU2021269417A9 (en) 2022-07-21
KR20150090072A (ko) 2015-08-05
AU2021269417A1 (en) 2021-12-16
US9958448B2 (en) 2018-05-01
BR112015009138A2 (pt) 2020-10-20
JP6404822B2 (ja) 2018-10-17

Similar Documents

Publication Publication Date Title
JP7442483B2 (ja) アプタマーおよびその使用
EP3039174B1 (en) Oligonucleotide probes and uses thereof
AU2013361323B2 (en) Compositions and methods for aptamer screening
US20220290124A1 (en) Oligonucleotide probes and uses thereof
US20210062191A1 (en) Oligonucleotide probes and uses thereof
AU2016229076A1 (en) Oligonucleotide probes and uses thereof
EP3577127A1 (en) Targeted oligonucleotides
US20210382059A1 (en) Aptamers and uses thereof
WO2019173799A1 (en) Oligonucleotide probes and uses thereof
EP3268476B1 (en) Oligonucleotide probes and uses thereof
HK40007814A (en) Oligonucleotide probes and uses thereof