DE69629576T2 - Verfahren zur herstellung von rekombinanten plasmiden - Google Patents
Verfahren zur herstellung von rekombinanten plasmiden Download PDFInfo
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- DE69629576T2 DE69629576T2 DE69629576T DE69629576T DE69629576T2 DE 69629576 T2 DE69629576 T2 DE 69629576T2 DE 69629576 T DE69629576 T DE 69629576T DE 69629576 T DE69629576 T DE 69629576T DE 69629576 T2 DE69629576 T2 DE 69629576T2
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- 238000012986 modification Methods 0.000 description 1
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- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229940023146 nucleic acid vaccine Drugs 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
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- 238000002360 preparation method Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54805995A | 1995-10-17 | 1995-10-17 | |
| US548059 | 1995-10-17 | ||
| US56497395A | 1995-11-30 | 1995-11-30 | |
| US564973 | 1995-11-30 | ||
| PCT/CA1996/000693 WO1997014805A2 (en) | 1995-10-17 | 1996-10-17 | Methods for production of recombinant plasmids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69629576D1 DE69629576D1 (de) | 2003-09-25 |
| DE69629576T2 true DE69629576T2 (de) | 2004-06-24 |
Family
ID=27068752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69629576T Expired - Fee Related DE69629576T2 (de) | 1995-10-17 | 1996-10-17 | Verfahren zur herstellung von rekombinanten plasmiden |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5922583A (enExample) |
| EP (1) | EP0856059B1 (enExample) |
| JP (1) | JPH11513562A (enExample) |
| AT (1) | ATE247713T1 (enExample) |
| AU (1) | AU7208896A (enExample) |
| CA (1) | CA2232234A1 (enExample) |
| DE (1) | DE69629576T2 (enExample) |
| TW (1) | TW514667B (enExample) |
| WO (1) | WO1997014805A2 (enExample) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE1006085A3 (fr) * | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
| WO1999058652A2 (en) * | 1998-05-07 | 1999-11-18 | Gx Biosystems A/S | Cytotoxin-based biological containment |
| WO2000028048A1 (en) * | 1998-11-09 | 2000-05-18 | Genecare Development Aps | Novel plasmids for use in medicine and method of producing same |
| US8076130B2 (en) * | 1998-12-02 | 2011-12-13 | University Of Maryland, Baltimore | Plasmid maintenance system for antigen delivery |
| US6413768B1 (en) | 1998-12-02 | 2002-07-02 | University Of Maryland | Expression plasmids |
| JP2003506007A (ja) | 1998-12-02 | 2003-02-18 | ユニヴァーシティ オブ メリーランド，ボルチモア | 抗原送達用プラスミド維持系 |
| US6423545B1 (en) * | 1999-07-08 | 2002-07-23 | Albert Einstein College Of Medicine Of Yeshiva University | Unmarked deletion mutants of mycobacteria and methods of using same |
| EP1111061A1 (en) * | 1999-12-20 | 2001-06-27 | Universite Libre De Bruxelles | Double selection vector |
| US9012141B2 (en) * | 2000-03-27 | 2015-04-21 | Advaxis, Inc. | Compositions and methods comprising KLK3 of FOLH1 antigen |
| JP5442177B2 (ja) * | 2001-02-23 | 2014-03-12 | ユニベルスィテ リーブル ドゥ ブリュッセル | 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法 |
| US7396822B2 (en) * | 2001-05-24 | 2008-07-08 | Vaxiion Therapeutics, Inc. | Immunogenic minicells and methods of use |
| US20030194798A1 (en) * | 2001-05-24 | 2003-10-16 | Surber Mark W. | Minicell compositions and methods |
| US20030224444A1 (en) * | 2002-02-25 | 2003-12-04 | Sabbadini Roger A. | Antibodies to native conformations of membrane proteins |
| US20030224369A1 (en) * | 2002-02-25 | 2003-12-04 | Surber Mark W. | Reverse screening and target identification with minicells |
| US20030207833A1 (en) * | 2002-02-25 | 2003-11-06 | Neil Berkley | Pharmaceutical compositions with minicells |
| CA2477194A1 (en) * | 2002-03-19 | 2003-09-25 | Universite Libre De Bruxelles | Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms |
| US20040005700A1 (en) * | 2002-05-28 | 2004-01-08 | Surber Mark W. | Poroplasts |
| AU2003260200A1 (en) * | 2002-09-03 | 2004-03-29 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
| US9309518B2 (en) | 2002-09-03 | 2016-04-12 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
| GB0327056D0 (en) * | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
| WO2006055024A2 (en) * | 2004-04-05 | 2006-05-26 | Vaxiion Therapeutics, Inc. | Minicells as vaccines |
| ES2684749T3 (es) * | 2004-08-13 | 2018-10-04 | The Trustees Of The University Of Pennsylvania | Métodos para construir vacunas sin resistencia a antibióticos |
| TWI311152B (en) * | 2004-09-17 | 2009-06-21 | Boehringer Ingelheim Rcv Gmbh & Co K | Host-vector system for antibiotic-free cole1 plasmid propagation |
| CN1912127B (zh) * | 2005-08-08 | 2011-05-25 | 百瑞全球有限公司 | 一种大肠杆菌表达载体及其应用 |
| EP2152889B1 (en) | 2007-05-29 | 2017-03-15 | Nature Technology Corporation | Vectors and methods for genetic immunization |
| US9650639B2 (en) | 2008-05-19 | 2017-05-16 | Advaxis, Inc. | Dual delivery system for heterologous antigens |
| US9017660B2 (en) | 2009-11-11 | 2015-04-28 | Advaxis, Inc. | Compositions and methods for prevention of escape mutation in the treatment of Her2/neu over-expressing tumors |
| ES2741730T3 (es) * | 2008-05-19 | 2020-02-12 | Advaxis Inc | Sistema de administración doble para antígenos heterólogos que comprende una cepa de Listeria recombinante atenuada por la mutación de dal/dat y la deleción de ActA que comprende una molécula de ácido nucleico que codifica una proteína de fusión de listeriolisina O - antígeno prostático específico |
| US20120135033A1 (en) * | 2008-05-19 | 2012-05-31 | Anu Wallecha | Multiple delivery system for heterologous antigens |
| EP2498808A4 (en) * | 2009-11-11 | 2014-01-08 | Advaxis | COMPOSITIONS AND METHOD FOR PREVENTING FLUID MUTATIONS IN THE TREATMENT OF TUMORS WITH HER2 / NEW OVEREXPRESSION |
| US10016617B2 (en) | 2009-11-11 | 2018-07-10 | The Trustees Of The University Of Pennsylvania | Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers |
| WO2012138377A2 (en) | 2010-10-01 | 2012-10-11 | Trustees Of The University Of Pennsylvania | The use of listeria vaccine vectors to reverse vaccine unresponsiveness in parasitically infected individuals |
| US10005820B2 (en) | 2011-02-15 | 2018-06-26 | Vaxiion Therapeutics, Llc | Therapeutic compositions and methods for antibody and Fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells |
| WO2012125551A1 (en) | 2011-03-11 | 2012-09-20 | Advaxis | Listeria-based adjuvants |
| EP2825195A4 (en) | 2012-03-12 | 2015-10-07 | Advaxis Inc | INHIBITION OF SUPPRESSOR CELL FUNCTION AFTER TREATMENT WITH A LISTERIAL VACCINE |
| EP2890404B1 (en) | 2012-08-29 | 2018-06-13 | Nature Technology Corporation | Dna plasmids with improved expression |
| WO2014077863A1 (en) | 2012-11-19 | 2014-05-22 | Nature Technology Corporation | Replicative minicircle vectors with improved expression |
| BR122021015417B1 (pt) | 2013-08-19 | 2022-11-08 | Syngulon Sa | Célula microbiana geneticamente engenheirada que compreende um ácido nucleico que codifica uma bacteriocina |
| WO2019057774A1 (en) | 2017-09-19 | 2019-03-28 | Deutsches Krebsforschungszentrum | NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS |
| DK3456821T4 (da) | 2017-09-19 | 2024-04-15 | Deutsches Krebsforsch | Ikke-integrerende dna-vektorer til genetisk modifikation af celler |
| CN111757890A (zh) | 2017-12-19 | 2020-10-09 | 辛格隆股份公司 | 发酵工艺 |
| JP7520719B2 (ja) | 2018-03-21 | 2024-07-23 | アルデブロン，エル．エル．シー． | 生成が改良されたウイルス及び非ウイルスのナノプラスミドベクター |
| WO2025160245A1 (en) | 2024-01-25 | 2025-07-31 | Aldevron, L.L.C. | Viral and non-viral nanoplasmid vectors with improved production |
| WO2025231315A2 (en) | 2024-05-03 | 2025-11-06 | Aldevron, L.L.C. | Bacterial host strains |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4968619A (en) * | 1976-09-27 | 1990-11-06 | Research Corporation | Modified microorganisms and method of preparing and using same |
| US4190495A (en) * | 1976-09-27 | 1980-02-26 | Research Corporation | Modified microorganisms and method of preparing and using same |
| US4735801A (en) * | 1982-09-07 | 1988-04-05 | Board Of Trustees Of Leland Stanford Jr. University | Novel non-reverting salmonella live vaccines |
| US4837151A (en) * | 1980-05-19 | 1989-06-06 | The Board Of Trustees Of The Leland Stanford Junior University, Stanford University | Live vaccines comprising two mutations and foreign antigen |
| US5077044A (en) * | 1980-05-19 | 1991-12-31 | The Board Of Trustees Of The Leland Stanford Jr. University | Novel non-reverting shigella live vaccines |
| US4550081A (en) * | 1980-05-19 | 1985-10-29 | The Board Of Trustees Of The Leland Stanford Jr. University | Non-reverting salmonella |
| US5210035A (en) * | 1980-05-19 | 1993-05-11 | Board Of Trustees Of Leland Stanford Jr. University | Non-reventing live vaccines |
| US4888170A (en) * | 1981-10-22 | 1989-12-19 | Research Corporation | Vaccines obtained from antigenic gene products of recombinant genes |
| DK410783D0 (da) * | 1982-09-16 | 1983-09-09 | Benzon A Salfred | Fremgangsmade til stabilisering af plasmider |
| US5702916A (en) * | 1982-09-16 | 1997-12-30 | Gx Biosystems A/S | Biological Containment |
| FR2568891B1 (fr) * | 1984-08-09 | 1988-05-27 | Transgene Sa | Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue |
| DK594084A (da) * | 1984-12-12 | 1986-06-13 | Novo Industri As | Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning |
| GB2177097A (en) * | 1985-06-18 | 1987-01-14 | Genencor Inc | Stable maintenance of nucleic acid in recombinant cells |
| DK275685D0 (da) * | 1985-06-18 | 1985-06-18 | Benzon As Alfred | Stabilisering af biologiske systemer |
| US5387744A (en) * | 1987-06-04 | 1995-02-07 | Washington University | Avirulent microbes and uses therefor: Salmonella typhi |
| IL86583A0 (en) * | 1987-06-04 | 1988-11-15 | Molecular Eng Ass | Vaccine containing a derivative of a microbe and method for the production thereof |
| US5294441A (en) * | 1987-06-04 | 1994-03-15 | Washington University | Avirulent microbes and uses therefor: salmonella typhi |
| ATE121785T1 (de) * | 1987-10-07 | 1995-05-15 | Univ Washington | Verfahren zur erhaltung eines erwünschten rekombinanten gens in einer genetischen zellpopulation. |
| IL91525A0 (en) * | 1988-09-06 | 1990-04-29 | Univ Washington | Transgenic plants,pharmaceutical compositions containing the same and methods for the preparation thereof |
| AU5355990A (en) * | 1989-03-31 | 1990-11-05 | Washington University | Avirulent microbes and uses therefor |
| AU645489B2 (en) * | 1989-03-31 | 1994-01-20 | Washington University | Vaccines containing avirulent phoP-type microorganisms |
| EP0474646A4 (en) * | 1989-03-31 | 1992-10-21 | Washington University | Bordetella vaccines |
| US5399346A (en) * | 1989-06-14 | 1995-03-21 | The United States Of America As Represented By The Department Of Health And Human Services | Gene therapy |
| AU6737190A (en) * | 1989-11-03 | 1991-05-31 | Washington University | Cross-protective salmonella vaccines |
| DE69131014T2 (de) * | 1990-11-21 | 1999-10-07 | Washington University, St. Louis | Rekombinante avirulente salmonella antifruchtbarkeitsimpfstoffe |
| AU6705594A (en) * | 1993-04-15 | 1994-11-08 | Washington University | Compositions of antigen containing recombinant salmonella, their use in anti-malarial vaccines and method for their preparation |
-
1996
- 1996-10-16 US US08/732,612 patent/US5922583A/en not_active Expired - Fee Related
- 1996-10-17 CA CA002232234A patent/CA2232234A1/en not_active Abandoned
- 1996-10-17 DE DE69629576T patent/DE69629576T2/de not_active Expired - Fee Related
- 1996-10-17 AT AT96933294T patent/ATE247713T1/de not_active IP Right Cessation
- 1996-10-17 JP JP9515377A patent/JPH11513562A/ja not_active Ceased
- 1996-10-17 WO PCT/CA1996/000693 patent/WO1997014805A2/en not_active Ceased
- 1996-10-17 EP EP96933294A patent/EP0856059B1/en not_active Expired - Lifetime
- 1996-10-17 AU AU72088/96A patent/AU7208896A/en not_active Abandoned
- 1996-12-24 TW TW085115998A patent/TW514667B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ATE247713T1 (de) | 2003-09-15 |
| TW514667B (en) | 2002-12-21 |
| JPH11513562A (ja) | 1999-11-24 |
| WO1997014805A3 (en) | 1997-06-12 |
| US5922583A (en) | 1999-07-13 |
| EP0856059B1 (en) | 2003-08-20 |
| DE69629576D1 (de) | 2003-09-25 |
| WO1997014805A2 (en) | 1997-04-24 |
| CA2232234A1 (en) | 1997-04-24 |
| EP0856059A1 (en) | 1998-08-05 |
| AU7208896A (en) | 1997-05-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8339 | Ceased/non-payment of the annual fee |