DE69629576T2 - Verfahren zur herstellung von rekombinanten plasmiden - Google Patents
Verfahren zur herstellung von rekombinanten plasmiden Download PDFInfo
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- DE69629576T2 DE69629576T2 DE69629576T DE69629576T DE69629576T2 DE 69629576 T2 DE69629576 T2 DE 69629576T2 DE 69629576 T DE69629576 T DE 69629576T DE 69629576 T DE69629576 T DE 69629576T DE 69629576 T2 DE69629576 T2 DE 69629576T2
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- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229940023146 nucleic acid vaccine Drugs 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000013492 plasmid preparation Methods 0.000 description 1
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- 230000003389 potentiating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US54805995A | 1995-10-17 | 1995-10-17 | |
| US548059 | 1995-10-17 | ||
| US56497395A | 1995-11-30 | 1995-11-30 | |
| US564973 | 1995-11-30 | ||
| PCT/CA1996/000693 WO1997014805A2 (en) | 1995-10-17 | 1996-10-17 | Methods for production of recombinant plasmids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69629576D1 DE69629576D1 (de) | 2003-09-25 |
| DE69629576T2 true DE69629576T2 (de) | 2004-06-24 |
Family
ID=27068752
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69629576T Expired - Fee Related DE69629576T2 (de) | 1995-10-17 | 1996-10-17 | Verfahren zur herstellung von rekombinanten plasmiden |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5922583A (enExample) |
| EP (1) | EP0856059B1 (enExample) |
| JP (1) | JPH11513562A (enExample) |
| AT (1) | ATE247713T1 (enExample) |
| AU (1) | AU7208896A (enExample) |
| CA (1) | CA2232234A1 (enExample) |
| DE (1) | DE69629576T2 (enExample) |
| TW (1) | TW514667B (enExample) |
| WO (1) | WO1997014805A2 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE1006085A3 (fr) * | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
| DE69942249D1 (de) * | 1998-05-07 | 2010-05-27 | Univ Bruxelles | Auf cytotoxinen basierendes biologisches einschlusssystem |
| AU1031900A (en) * | 1998-11-09 | 2000-05-29 | Genecare Development Aps | Novel plasmids for use in medicine and method of producing same |
| EP1135025B1 (en) * | 1998-12-02 | 2007-04-25 | University of Maryland, Baltimore | Plasmid maintenance system for antigen delivery |
| US6413768B1 (en) | 1998-12-02 | 2002-07-02 | University Of Maryland | Expression plasmids |
| US8076130B2 (en) * | 1998-12-02 | 2011-12-13 | University Of Maryland, Baltimore | Plasmid maintenance system for antigen delivery |
| US6423545B1 (en) * | 1999-07-08 | 2002-07-23 | Albert Einstein College Of Medicine Of Yeshiva University | Unmarked deletion mutants of mycobacteria and methods of using same |
| EP1111061A1 (en) * | 1999-12-20 | 2001-06-27 | Universite Libre De Bruxelles | Double selection vector |
| US9012141B2 (en) * | 2000-03-27 | 2015-04-21 | Advaxis, Inc. | Compositions and methods comprising KLK3 of FOLH1 antigen |
| EP1362110B1 (en) | 2001-02-23 | 2012-09-12 | Université Libre de Bruxelles | Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to a toxic molecule |
| US7396822B2 (en) | 2001-05-24 | 2008-07-08 | Vaxiion Therapeutics, Inc. | Immunogenic minicells and methods of use |
| US20030194798A1 (en) * | 2001-05-24 | 2003-10-16 | Surber Mark W. | Minicell compositions and methods |
| US20030224369A1 (en) * | 2002-02-25 | 2003-12-04 | Surber Mark W. | Reverse screening and target identification with minicells |
| US20030224444A1 (en) * | 2002-02-25 | 2003-12-04 | Sabbadini Roger A. | Antibodies to native conformations of membrane proteins |
| US20030207833A1 (en) * | 2002-02-25 | 2003-11-06 | Neil Berkley | Pharmaceutical compositions with minicells |
| JP4564754B2 (ja) * | 2002-03-19 | 2010-10-20 | ユニヴェルシテ リブル ドゥ ブリュッセル | 遺伝的に改変された真核細胞又は生物の選択のための毒物／解毒剤遺伝子系 |
| US20040005700A1 (en) * | 2002-05-28 | 2004-01-08 | Surber Mark W. | Poroplasts |
| US9309518B2 (en) | 2002-09-03 | 2016-04-12 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
| EP1556485B1 (en) * | 2002-09-03 | 2008-01-09 | Université Libre de Bruxelles | Reversible, parallel and multitask cloning method and kit |
| GB0327056D0 (en) * | 2003-11-20 | 2003-12-24 | Cobra Biolog Ltd | Plasmid maintenance |
| WO2006055024A2 (en) * | 2004-04-05 | 2006-05-26 | Vaxiion Therapeutics, Inc. | Minicells as vaccines |
| ES2543079T3 (es) | 2004-08-13 | 2015-08-14 | The Trustees Of The University Of Pennsylvania | Métodos para construir vacunas sin resistencia antibiótica |
| TWI311152B (en) * | 2004-09-17 | 2009-06-21 | Boehringer Ingelheim Rcv Gmbh & Co K | Host-vector system for antibiotic-free cole1 plasmid propagation |
| CN1912127B (zh) * | 2005-08-08 | 2011-05-25 | 百瑞全球有限公司 | 一种大肠杆菌表达载体及其应用 |
| AU2008262478B9 (en) | 2007-05-29 | 2014-06-19 | Aldevron, L.L.C. | Vectors and methods for genetic immunization |
| JP5757863B2 (ja) * | 2008-05-19 | 2015-08-05 | アドバクシス インコーポレイテッド | 異種抗原のための二重送達システム |
| US9017660B2 (en) | 2009-11-11 | 2015-04-28 | Advaxis, Inc. | Compositions and methods for prevention of escape mutation in the treatment of Her2/neu over-expressing tumors |
| US20120135033A1 (en) * | 2008-05-19 | 2012-05-31 | Anu Wallecha | Multiple delivery system for heterologous antigens |
| US9650639B2 (en) | 2008-05-19 | 2017-05-16 | Advaxis, Inc. | Dual delivery system for heterologous antigens |
| JP5985397B2 (ja) * | 2009-11-11 | 2016-09-06 | アドバクシス インコーポレイテッド | 組換えリステリア株およびそれを含む免疫原性組成物 |
| US10016617B2 (en) | 2009-11-11 | 2018-07-10 | The Trustees Of The University Of Pennsylvania | Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers |
| CN107412756A (zh) | 2010-10-01 | 2017-12-01 | 宾夕法尼亚大学理事会 | 李斯特菌疫苗载体用于在寄生虫感染的个体中扭转免疫无应答的用途 |
| DK2675474T3 (en) | 2011-02-15 | 2019-04-23 | Vaxiion Therapeutics Llc | THERAPEUTIC COMPOSITIONS AND PROCEDURES FOR TARGETED ADMINISTRATION OF BIOACTIVE MOLECULES BY BACK-TERIAL MINICLES BASED ON ANTIBODY AND FC-CONTAINING TAR-GETING MOLECULES |
| CA2829960A1 (en) | 2011-03-11 | 2012-09-20 | John Rothman | Listeria-based adjuvants |
| BR112014022662A2 (pt) | 2012-03-12 | 2017-10-03 | Advaxis Inc | Inibição da função de célula supressora seguindo tratamento de vacina de listeria |
| US9550998B2 (en) | 2012-08-29 | 2017-01-24 | Nature Technology Corporation | DNA plasmids with improved expression |
| US20150322439A1 (en) | 2012-11-19 | 2015-11-12 | Nature Technology Corporation | Replicative minicircle vectors with improved expression |
| CN110564663B (zh) | 2013-08-19 | 2023-03-10 | 辛格隆股份公司 | 微生物的受控生长 |
| WO2019057774A1 (en) | 2017-09-19 | 2019-03-28 | Deutsches Krebsforschungszentrum | NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS |
| EP3456821B2 (en) | 2017-09-19 | 2024-01-24 | Deutsches Krebsforschungszentrum | Non-integrating dna vectors for the genetic modification of cells |
| JP2021506328A (ja) | 2017-12-19 | 2021-02-22 | シンギュロン・ソシエテ・アノニム | 発酵プロセス |
| AU2019240068B2 (en) | 2018-03-21 | 2025-03-13 | Aldevron, L.L.C. | Viral and non-viral nanoplasmid vectors with improved production |
| CN122497758A (zh) | 2024-01-25 | 2026-07-31 | 阿尔德夫隆有限责任公司 | 具有改进的生产性能的病毒和非病毒纳米质粒载体 |
| WO2025231315A2 (en) | 2024-05-03 | 2025-11-06 | Aldevron, L.L.C. | Bacterial host strains |
| WO2026076187A1 (en) | 2024-10-03 | 2026-04-09 | Aldevron, L.L.C. | Compositions and methods for microbial production of antibiotic-free closed-ended linear dna |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4190495A (en) * | 1976-09-27 | 1980-02-26 | Research Corporation | Modified microorganisms and method of preparing and using same |
| US4968619A (en) * | 1976-09-27 | 1990-11-06 | Research Corporation | Modified microorganisms and method of preparing and using same |
| US4735801A (en) * | 1982-09-07 | 1988-04-05 | Board Of Trustees Of Leland Stanford Jr. University | Novel non-reverting salmonella live vaccines |
| US5210035A (en) * | 1980-05-19 | 1993-05-11 | Board Of Trustees Of Leland Stanford Jr. University | Non-reventing live vaccines |
| US5077044A (en) * | 1980-05-19 | 1991-12-31 | The Board Of Trustees Of The Leland Stanford Jr. University | Novel non-reverting shigella live vaccines |
| US4837151A (en) * | 1980-05-19 | 1989-06-06 | The Board Of Trustees Of The Leland Stanford Junior University, Stanford University | Live vaccines comprising two mutations and foreign antigen |
| US4550081A (en) * | 1980-05-19 | 1985-10-29 | The Board Of Trustees Of The Leland Stanford Jr. University | Non-reverting salmonella |
| US4888170A (en) * | 1981-10-22 | 1989-12-19 | Research Corporation | Vaccines obtained from antigenic gene products of recombinant genes |
| US5702916A (en) * | 1982-09-16 | 1997-12-30 | Gx Biosystems A/S | Biological Containment |
| DK410783D0 (da) * | 1982-09-16 | 1983-09-09 | Benzon A Salfred | Fremgangsmade til stabilisering af plasmider |
| FR2568891B1 (fr) * | 1984-08-09 | 1988-05-27 | Transgene Sa | Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue |
| DK594084A (da) * | 1984-12-12 | 1986-06-13 | Novo Industri As | Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning |
| DK275685D0 (da) * | 1985-06-18 | 1985-06-18 | Benzon As Alfred | Stabilisering af biologiske systemer |
| GB2177097A (en) * | 1985-06-18 | 1987-01-14 | Genencor Inc | Stable maintenance of nucleic acid in recombinant cells |
| US5387744A (en) * | 1987-06-04 | 1995-02-07 | Washington University | Avirulent microbes and uses therefor: Salmonella typhi |
| US5294441A (en) * | 1987-06-04 | 1994-03-15 | Washington University | Avirulent microbes and uses therefor: salmonella typhi |
| IL86583A0 (en) * | 1987-06-04 | 1988-11-15 | Molecular Eng Ass | Vaccine containing a derivative of a microbe and method for the production thereof |
| AU637969B2 (en) * | 1987-10-07 | 1993-06-17 | Washington University | A method of maintaining a desired recombinant gene in a genetic population of cells |
| CA1339307C (en) * | 1988-09-06 | 1997-08-19 | Roy Curtiss, Iii | Oral immunization by transgenic plants |
| JPH04504656A (ja) * | 1989-03-31 | 1992-08-20 | ワシントン ユニバーシティー | ボルデテラワクチン |
| JPH04506000A (ja) * | 1989-03-31 | 1992-10-22 | ワシントン・ユニバーシティ | 無毒性微生物類およびそれについての使用 |
| DE69027313T2 (de) * | 1989-03-31 | 1997-01-23 | Univ Washington | Impfstoffe enthaltende avirulente phop-type mikroorganismen |
| US5399346A (en) * | 1989-06-14 | 1995-03-21 | The United States Of America As Represented By The Department Of Health And Human Services | Gene therapy |
| DE69032408T2 (de) * | 1989-11-03 | 1998-10-08 | Univ Washington | Kreuzschützende salmonella impfstoffe |
| AU9094191A (en) * | 1990-11-21 | 1992-06-25 | Washington University | Recombinant avirulent salmonella antifertility vaccines |
| AU6705594A (en) * | 1993-04-15 | 1994-11-08 | Washington University | Compositions of antigen containing recombinant salmonella, their use in anti-malarial vaccines and method for their preparation |
-
1996
- 1996-10-16 US US08/732,612 patent/US5922583A/en not_active Expired - Fee Related
- 1996-10-17 JP JP9515377A patent/JPH11513562A/ja not_active Ceased
- 1996-10-17 AU AU72088/96A patent/AU7208896A/en not_active Abandoned
- 1996-10-17 EP EP96933294A patent/EP0856059B1/en not_active Expired - Lifetime
- 1996-10-17 CA CA002232234A patent/CA2232234A1/en not_active Abandoned
- 1996-10-17 DE DE69629576T patent/DE69629576T2/de not_active Expired - Fee Related
- 1996-10-17 AT AT96933294T patent/ATE247713T1/de not_active IP Right Cessation
- 1996-10-17 WO PCT/CA1996/000693 patent/WO1997014805A2/en not_active Ceased
- 1996-12-24 TW TW085115998A patent/TW514667B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| WO1997014805A3 (en) | 1997-06-12 |
| ATE247713T1 (de) | 2003-09-15 |
| EP0856059B1 (en) | 2003-08-20 |
| EP0856059A1 (en) | 1998-08-05 |
| US5922583A (en) | 1999-07-13 |
| WO1997014805A2 (en) | 1997-04-24 |
| DE69629576D1 (de) | 2003-09-25 |
| JPH11513562A (ja) | 1999-11-24 |
| AU7208896A (en) | 1997-05-07 |
| TW514667B (en) | 2002-12-21 |
| CA2232234A1 (en) | 1997-04-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8339 | Ceased/non-payment of the annual fee |