JPH11513562A - 組換えプラスミドの生産方法 - Google Patents

組換えプラスミドの生産方法

Info

Publication number
JPH11513562A
JPH11513562A JP9515377A JP51537797A JPH11513562A JP H11513562 A JPH11513562 A JP H11513562A JP 9515377 A JP9515377 A JP 9515377A JP 51537797 A JP51537797 A JP 51537797A JP H11513562 A JPH11513562 A JP H11513562A
Authority
JP
Japan
Prior art keywords
plasmid
cells
cell
gene
bacterial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP9515377A
Other languages
English (en)
Japanese (ja)
Other versions
JPH11513562A5 (enExample
Inventor
エイ． モージー，モハマド
Original Assignee
バイオスター，インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by バイオスター，インコーポレイテッド filed Critical バイオスター，インコーポレイテッド
Publication of JPH11513562A publication Critical patent/JPH11513562A/ja
Publication of JPH11513562A5 publication Critical patent/JPH11513562A5/ja
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP9515377A 1995-10-17 1996-10-17 組換えプラスミドの生産方法 Ceased JPH11513562A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US54805995A 1995-10-17 1995-10-17
US08/548,059 1995-10-17
US56497395A 1995-11-30 1995-11-30
US08/564,973 1995-11-30
PCT/CA1996/000693 WO1997014805A2 (en) 1995-10-17 1996-10-17 Methods for production of recombinant plasmids

Publications (2)

Publication Number Publication Date
JPH11513562A true JPH11513562A (ja) 1999-11-24
JPH11513562A5 JPH11513562A5 (enExample) 2004-10-14

Family

ID=27068752

Family Applications (1)

Application Number Title Priority Date Filing Date
JP9515377A Ceased JPH11513562A (ja) 1995-10-17 1996-10-17 組換えプラスミドの生産方法

Country Status (9)

Country Link
US (1) US5922583A (enExample)
EP (1) EP0856059B1 (enExample)
JP (1) JPH11513562A (enExample)
AT (1) ATE247713T1 (enExample)
AU (1) AU7208896A (enExample)
CA (1) CA2232234A1 (enExample)
DE (1) DE69629576T2 (enExample)
TW (1) TW514667B (enExample)
WO (1) WO1997014805A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007511236A (ja) * 2003-11-20 2007-05-10 コブラ バイオロジックス リミテッド プラスミドの維持
JP2008512998A (ja) * 2004-09-17 2008-05-01 ベーリンガー インゲルハイム オーストリア ゲゼルシャフト ミット ベシュレンクテル ハフツング 抗生物質フリーのＣｏ１Ｅ１プラスミドを増殖するための宿主ベクター系
JP2014064579A (ja) * 2001-02-23 2014-04-17 Universite Libre De Bruxelles 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法

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BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
EP2180050B1 (en) * 1998-05-07 2016-04-20 Universite Libre De Bruxelles Cytotoxin-based biological containment
AU1031900A (en) * 1998-11-09 2000-05-29 Genecare Development Aps Novel plasmids for use in medicine and method of producing same
ES2286905T3 (es) 1998-12-02 2007-12-01 University Of Maryland, Baltimore Sistema de estabilizacion de plasmidos para el suministro de antigenos.
US6413768B1 (en) 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
US8076130B2 (en) * 1998-12-02 2011-12-13 University Of Maryland, Baltimore Plasmid maintenance system for antigen delivery
US6423545B1 (en) * 1999-07-08 2002-07-23 Albert Einstein College Of Medicine Of Yeshiva University Unmarked deletion mutants of mycobacteria and methods of using same
EP1111061A1 (en) * 1999-12-20 2001-06-27 Universite Libre De Bruxelles Double selection vector
US9012141B2 (en) 2000-03-27 2015-04-21 Advaxis, Inc. Compositions and methods comprising KLK3 of FOLH1 antigen
US7396822B2 (en) 2001-05-24 2008-07-08 Vaxiion Therapeutics, Inc. Immunogenic minicells and methods of use
US20030194798A1 (en) 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
US20030207833A1 (en) * 2002-02-25 2003-11-06 Neil Berkley Pharmaceutical compositions with minicells
US20030224369A1 (en) * 2002-02-25 2003-12-04 Surber Mark W. Reverse screening and target identification with minicells
US20030224444A1 (en) * 2002-02-25 2003-12-04 Sabbadini Roger A. Antibodies to native conformations of membrane proteins
US20050260585A1 (en) * 2002-03-19 2005-11-24 Cedric Szpirer Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
US20040005700A1 (en) * 2002-05-28 2004-01-08 Surber Mark W. Poroplasts
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
AU2003260200A1 (en) * 2002-09-03 2004-03-29 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
US20060002956A1 (en) * 2004-04-05 2006-01-05 Surber Mark W Minicells as vaccines
CA2577306A1 (en) 2004-08-13 2006-02-16 The Trustees Of The University Of Pennsylvania Methods for constructing antibiotic resistance free vaccines
CN1912127B (zh) * 2005-08-08 2011-05-25 百瑞全球有限公司 一种大肠杆菌表达载体及其应用
EP2333091B1 (en) 2007-05-29 2017-06-21 Nature Technology Corporation Vectors and methods for genetic immunization
US20120135033A1 (en) * 2008-05-19 2012-05-31 Anu Wallecha Multiple delivery system for heterologous antigens
EP2853269B1 (en) 2008-05-19 2019-05-01 Advaxis, Inc. Dual delivery system for heterologous antigens comprising a recombinant Listeria strain attenuated by mutation of dal/dat and deletion of ActA comprising a nucleic acid molecule encoding an listeriolysin O - prostate specific anigen fusion protein
US9017660B2 (en) 2009-11-11 2015-04-28 Advaxis, Inc. Compositions and methods for prevention of escape mutation in the treatment of Her2/neu over-expressing tumors
US9650639B2 (en) 2008-05-19 2017-05-16 Advaxis, Inc. Dual delivery system for heterologous antigens
US10016617B2 (en) 2009-11-11 2018-07-10 The Trustees Of The University Of Pennsylvania Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers
EP2498808A4 (en) * 2009-11-11 2014-01-08 Advaxis COMPOSITIONS AND METHOD FOR PREVENTING FLUID MUTATIONS IN THE TREATMENT OF TUMORS WITH HER2 / NEW OVEREXPRESSION
CN103282048B (zh) 2010-10-01 2017-05-17 宾夕法尼亚大学理事会 李斯特菌疫苗载体用于在寄生虫感染的个体中扭转免疫无应答的用途
EP3527222B1 (en) 2011-02-15 2021-04-07 Vaxiion Therapeutics, LLC Therapeutic compositions and methods for antibody and fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells
CA2829960A1 (en) 2011-03-11 2012-09-20 John Rothman Listeria-based adjuvants
KR20140134695A (ko) 2012-03-12 2014-11-24 어드박시스, 인크. 리스테리아 백신 치료 후 억제 세포 기능 저해
AU2013309488A1 (en) 2012-08-29 2015-03-05 Nature Technology Corporation DNA plasmids with improved expression
WO2014077863A1 (en) 2012-11-19 2014-05-22 Nature Technology Corporation Replicative minicircle vectors with improved expression
BR112016003533B1 (pt) 2013-08-19 2021-11-03 Syngulon Sa Célula microbiana geneticamente modificada e método para controlar o crescimento de uma segunda célula microbiana em um meio de cultura
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
FI3456821T4 (fi) 2017-09-19 2024-04-24 Deutsches Krebsforsch Ei-integroituvia DNA-vektoreita solujen geneettistä muokkausta varten
WO2019121983A1 (en) 2017-12-19 2019-06-27 Syngulon S.A. Fermentation process
AU2019240068B2 (en) 2018-03-21 2025-03-13 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025160245A1 (en) 2024-01-25 2025-07-31 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025231315A2 (en) 2024-05-03 2025-11-06 Aldevron, L.L.C. Bacterial host strains
WO2026076187A1 (en) 2024-10-03 2026-04-09 Aldevron, L.L.C. Compositions and methods for microbial production of antibiotic-free closed-ended linear dna

Family Cites Families (26)

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US4190495A (en) * 1976-09-27 1980-02-26 Research Corporation Modified microorganisms and method of preparing and using same
US4968619A (en) * 1976-09-27 1990-11-06 Research Corporation Modified microorganisms and method of preparing and using same
US5077044A (en) * 1980-05-19 1991-12-31 The Board Of Trustees Of The Leland Stanford Jr. University Novel non-reverting shigella live vaccines
US4735801A (en) * 1982-09-07 1988-04-05 Board Of Trustees Of Leland Stanford Jr. University Novel non-reverting salmonella live vaccines
US4837151A (en) * 1980-05-19 1989-06-06 The Board Of Trustees Of The Leland Stanford Junior University, Stanford University Live vaccines comprising two mutations and foreign antigen
US5210035A (en) * 1980-05-19 1993-05-11 Board Of Trustees Of Leland Stanford Jr. University Non-reventing live vaccines
US4550081A (en) * 1980-05-19 1985-10-29 The Board Of Trustees Of The Leland Stanford Jr. University Non-reverting salmonella
US4888170A (en) * 1981-10-22 1989-12-19 Research Corporation Vaccines obtained from antigenic gene products of recombinant genes
US5702916A (en) * 1982-09-16 1997-12-30 Gx Biosystems A/S Biological Containment
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
FR2568891B1 (fr) * 1984-08-09 1988-05-27 Transgene Sa Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
GB2177097A (en) * 1985-06-18 1987-01-14 Genencor Inc Stable maintenance of nucleic acid in recombinant cells
DK275685D0 (da) * 1985-06-18 1985-06-18 Benzon As Alfred Stabilisering af biologiske systemer
IL86583A0 (en) * 1987-06-04 1988-11-15 Molecular Eng Ass Vaccine containing a derivative of a microbe and method for the production thereof
US5387744A (en) * 1987-06-04 1995-02-07 Washington University Avirulent microbes and uses therefor: Salmonella typhi
US5294441A (en) * 1987-06-04 1994-03-15 Washington University Avirulent microbes and uses therefor: salmonella typhi
JPH04501351A (ja) * 1987-10-07 1992-03-12 ワシントン ユニバーシティ 細胞の遺伝的集団において所望の組換え遺伝子を維持する方法
IL91525A0 (en) * 1988-09-06 1990-04-29 Univ Washington Transgenic plants,pharmaceutical compositions containing the same and methods for the preparation thereof
WO1990012086A1 (en) * 1989-03-31 1990-10-18 Washington University Bordetella vaccines
WO1990011687A1 (en) * 1989-03-31 1990-10-18 Washington University VACCINES CONTAINING AVIRULENT phoP-TYPE MICROORGANISMS
EP0465561A4 (en) * 1989-03-31 1992-04-08 Washington University Avirulent microbes and uses therefor
US5399346A (en) * 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
AU6737190A (en) * 1989-11-03 1991-05-31 Washington University Cross-protective salmonella vaccines
AU9094191A (en) * 1990-11-21 1992-06-25 Washington University Recombinant avirulent salmonella antifertility vaccines
WO1994024291A2 (en) * 1993-04-15 1994-10-27 Washington University Compositions of antigen containing recombinant salmonella, their use in anti-malarial vaccines and method for their preparation

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014064579A (ja) * 2001-02-23 2014-04-17 Universite Libre De Bruxelles 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法
JP2007511236A (ja) * 2003-11-20 2007-05-10 コブラ バイオロジックス リミテッド プラスミドの維持
JP2008512998A (ja) * 2004-09-17 2008-05-01 ベーリンガー インゲルハイム オーストリア ゲゼルシャフト ミット ベシュレンクテル ハフツング 抗生物質フリーのＣｏ１Ｅ１プラスミドを増殖するための宿主ベクター系

Also Published As

Publication number Publication date
CA2232234A1 (en) 1997-04-24
WO1997014805A3 (en) 1997-06-12
DE69629576T2 (de) 2004-06-24
TW514667B (en) 2002-12-21
US5922583A (en) 1999-07-13
ATE247713T1 (de) 2003-09-15
EP0856059B1 (en) 2003-08-20
DE69629576D1 (de) 2003-09-25
WO1997014805A2 (en) 1997-04-24
EP0856059A1 (en) 1998-08-05
AU7208896A (en) 1997-05-07

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