ATE247713T1 - Verfahren zur herstellung von rekombinanten plasmiden - Google Patents

Verfahren zur herstellung von rekombinanten plasmiden

Info

Publication number
ATE247713T1
ATE247713T1 AT96933294T AT96933294T ATE247713T1 AT E247713 T1 ATE247713 T1 AT E247713T1 AT 96933294 T AT96933294 T AT 96933294T AT 96933294 T AT96933294 T AT 96933294T AT E247713 T1 ATE247713 T1 AT E247713T1
Authority
AT
Austria
Prior art keywords
cells
genetic material
plasmids
culture system
toxic
Prior art date
Application number
AT96933294T
Other languages
German (de)
English (en)
Inventor
Mohamad A Morsey
Original Assignee
Novartis Pharma Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Pharma Gmbh filed Critical Novartis Pharma Gmbh
Application granted granted Critical
Publication of ATE247713T1 publication Critical patent/ATE247713T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT96933294T 1995-10-17 1996-10-17 Verfahren zur herstellung von rekombinanten plasmiden ATE247713T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US54805995A 1995-10-17 1995-10-17
US56497395A 1995-11-30 1995-11-30
PCT/CA1996/000693 WO1997014805A2 (en) 1995-10-17 1996-10-17 Methods for production of recombinant plasmids

Publications (1)

Publication Number Publication Date
ATE247713T1 true ATE247713T1 (de) 2003-09-15

Family

ID=27068752

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96933294T ATE247713T1 (de) 1995-10-17 1996-10-17 Verfahren zur herstellung von rekombinanten plasmiden

Country Status (9)

Country Link
US (1) US5922583A (enExample)
EP (1) EP0856059B1 (enExample)
JP (1) JPH11513562A (enExample)
AT (1) ATE247713T1 (enExample)
AU (1) AU7208896A (enExample)
CA (1) CA2232234A1 (enExample)
DE (1) DE69629576T2 (enExample)
TW (1) TW514667B (enExample)
WO (1) WO1997014805A2 (enExample)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
WO1999058652A2 (en) * 1998-05-07 1999-11-18 Gx Biosystems A/S Cytotoxin-based biological containment
WO2000028048A1 (en) * 1998-11-09 2000-05-18 Genecare Development Aps Novel plasmids for use in medicine and method of producing same
US8076130B2 (en) * 1998-12-02 2011-12-13 University Of Maryland, Baltimore Plasmid maintenance system for antigen delivery
US6413768B1 (en) 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
JP2003506007A (ja) 1998-12-02 2003-02-18 ユニヴァーシティ オブ メリーランド，ボルチモア 抗原送達用プラスミド維持系
US6423545B1 (en) * 1999-07-08 2002-07-23 Albert Einstein College Of Medicine Of Yeshiva University Unmarked deletion mutants of mycobacteria and methods of using same
EP1111061A1 (en) * 1999-12-20 2001-06-27 Universite Libre De Bruxelles Double selection vector
US9012141B2 (en) * 2000-03-27 2015-04-21 Advaxis, Inc. Compositions and methods comprising KLK3 of FOLH1 antigen
JP5442177B2 (ja) * 2001-02-23 2014-03-12 ユニベルスィテ リーブル ドゥ ブリュッセル 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法
US7396822B2 (en) * 2001-05-24 2008-07-08 Vaxiion Therapeutics, Inc. Immunogenic minicells and methods of use
US20030194798A1 (en) * 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
US20030224444A1 (en) * 2002-02-25 2003-12-04 Sabbadini Roger A. Antibodies to native conformations of membrane proteins
US20030224369A1 (en) * 2002-02-25 2003-12-04 Surber Mark W. Reverse screening and target identification with minicells
US20030207833A1 (en) * 2002-02-25 2003-11-06 Neil Berkley Pharmaceutical compositions with minicells
CA2477194A1 (en) * 2002-03-19 2003-09-25 Universite Libre De Bruxelles Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
US20040005700A1 (en) * 2002-05-28 2004-01-08 Surber Mark W. Poroplasts
AU2003260200A1 (en) * 2002-09-03 2004-03-29 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
WO2006055024A2 (en) * 2004-04-05 2006-05-26 Vaxiion Therapeutics, Inc. Minicells as vaccines
ES2684749T3 (es) * 2004-08-13 2018-10-04 The Trustees Of The University Of Pennsylvania Métodos para construir vacunas sin resistencia a antibióticos
TWI311152B (en) * 2004-09-17 2009-06-21 Boehringer Ingelheim Rcv Gmbh & Co K Host-vector system for antibiotic-free cole1 plasmid propagation
CN1912127B (zh) * 2005-08-08 2011-05-25 百瑞全球有限公司 一种大肠杆菌表达载体及其应用
EP2152889B1 (en) 2007-05-29 2017-03-15 Nature Technology Corporation Vectors and methods for genetic immunization
US9650639B2 (en) 2008-05-19 2017-05-16 Advaxis, Inc. Dual delivery system for heterologous antigens
US9017660B2 (en) 2009-11-11 2015-04-28 Advaxis, Inc. Compositions and methods for prevention of escape mutation in the treatment of Her2/neu over-expressing tumors
ES2741730T3 (es) * 2008-05-19 2020-02-12 Advaxis Inc Sistema de administración doble para antígenos heterólogos que comprende una cepa de Listeria recombinante atenuada por la mutación de dal/dat y la deleción de ActA que comprende una molécula de ácido nucleico que codifica una proteína de fusión de listeriolisina O - antígeno prostático específico
US20120135033A1 (en) * 2008-05-19 2012-05-31 Anu Wallecha Multiple delivery system for heterologous antigens
EP2498808A4 (en) * 2009-11-11 2014-01-08 Advaxis COMPOSITIONS AND METHOD FOR PREVENTING FLUID MUTATIONS IN THE TREATMENT OF TUMORS WITH HER2 / NEW OVEREXPRESSION
US10016617B2 (en) 2009-11-11 2018-07-10 The Trustees Of The University Of Pennsylvania Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers
WO2012138377A2 (en) 2010-10-01 2012-10-11 Trustees Of The University Of Pennsylvania The use of listeria vaccine vectors to reverse vaccine unresponsiveness in parasitically infected individuals
US10005820B2 (en) 2011-02-15 2018-06-26 Vaxiion Therapeutics, Llc Therapeutic compositions and methods for antibody and Fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells
WO2012125551A1 (en) 2011-03-11 2012-09-20 Advaxis Listeria-based adjuvants
EP2825195A4 (en) 2012-03-12 2015-10-07 Advaxis Inc INHIBITION OF SUPPRESSOR CELL FUNCTION AFTER TREATMENT WITH A LISTERIAL VACCINE
EP2890404B1 (en) 2012-08-29 2018-06-13 Nature Technology Corporation Dna plasmids with improved expression
WO2014077863A1 (en) 2012-11-19 2014-05-22 Nature Technology Corporation Replicative minicircle vectors with improved expression
BR122021015417B1 (pt) 2013-08-19 2022-11-08 Syngulon Sa Célula microbiana geneticamente engenheirada que compreende um ácido nucleico que codifica uma bacteriocina
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
DK3456821T4 (da) 2017-09-19 2024-04-15 Deutsches Krebsforsch Ikke-integrerende dna-vektorer til genetisk modifikation af celler
CN111757890A (zh) 2017-12-19 2020-10-09 辛格隆股份公司 发酵工艺
JP7520719B2 (ja) 2018-03-21 2024-07-23 アルデブロン，エル．エル．シー． 生成が改良されたウイルス及び非ウイルスのナノプラスミドベクター
WO2025160245A1 (en) 2024-01-25 2025-07-31 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025231315A2 (en) 2024-05-03 2025-11-06 Aldevron, L.L.C. Bacterial host strains

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4968619A (en) * 1976-09-27 1990-11-06 Research Corporation Modified microorganisms and method of preparing and using same
US4190495A (en) * 1976-09-27 1980-02-26 Research Corporation Modified microorganisms and method of preparing and using same
US4735801A (en) * 1982-09-07 1988-04-05 Board Of Trustees Of Leland Stanford Jr. University Novel non-reverting salmonella live vaccines
US4837151A (en) * 1980-05-19 1989-06-06 The Board Of Trustees Of The Leland Stanford Junior University, Stanford University Live vaccines comprising two mutations and foreign antigen
US5077044A (en) * 1980-05-19 1991-12-31 The Board Of Trustees Of The Leland Stanford Jr. University Novel non-reverting shigella live vaccines
US4550081A (en) * 1980-05-19 1985-10-29 The Board Of Trustees Of The Leland Stanford Jr. University Non-reverting salmonella
US5210035A (en) * 1980-05-19 1993-05-11 Board Of Trustees Of Leland Stanford Jr. University Non-reventing live vaccines
US4888170A (en) * 1981-10-22 1989-12-19 Research Corporation Vaccines obtained from antigenic gene products of recombinant genes
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
US5702916A (en) * 1982-09-16 1997-12-30 Gx Biosystems A/S Biological Containment
FR2568891B1 (fr) * 1984-08-09 1988-05-27 Transgene Sa Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
GB2177097A (en) * 1985-06-18 1987-01-14 Genencor Inc Stable maintenance of nucleic acid in recombinant cells
DK275685D0 (da) * 1985-06-18 1985-06-18 Benzon As Alfred Stabilisering af biologiske systemer
US5387744A (en) * 1987-06-04 1995-02-07 Washington University Avirulent microbes and uses therefor: Salmonella typhi
IL86583A0 (en) * 1987-06-04 1988-11-15 Molecular Eng Ass Vaccine containing a derivative of a microbe and method for the production thereof
US5294441A (en) * 1987-06-04 1994-03-15 Washington University Avirulent microbes and uses therefor: salmonella typhi
ATE121785T1 (de) * 1987-10-07 1995-05-15 Univ Washington Verfahren zur erhaltung eines erwünschten rekombinanten gens in einer genetischen zellpopulation.
IL91525A0 (en) * 1988-09-06 1990-04-29 Univ Washington Transgenic plants,pharmaceutical compositions containing the same and methods for the preparation thereof
AU5355990A (en) * 1989-03-31 1990-11-05 Washington University Avirulent microbes and uses therefor
AU645489B2 (en) * 1989-03-31 1994-01-20 Washington University Vaccines containing avirulent phoP-type microorganisms
EP0474646A4 (en) * 1989-03-31 1992-10-21 Washington University Bordetella vaccines
US5399346A (en) * 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
AU6737190A (en) * 1989-11-03 1991-05-31 Washington University Cross-protective salmonella vaccines
DE69131014T2 (de) * 1990-11-21 1999-10-07 Washington University, St. Louis Rekombinante avirulente salmonella antifruchtbarkeitsimpfstoffe
AU6705594A (en) * 1993-04-15 1994-11-08 Washington University Compositions of antigen containing recombinant salmonella, their use in anti-malarial vaccines and method for their preparation

Also Published As

Publication number Publication date
TW514667B (en) 2002-12-21
JPH11513562A (ja) 1999-11-24
WO1997014805A3 (en) 1997-06-12
DE69629576T2 (de) 2004-06-24
US5922583A (en) 1999-07-13
EP0856059B1 (en) 2003-08-20
DE69629576D1 (de) 2003-09-25
WO1997014805A2 (en) 1997-04-24
CA2232234A1 (en) 1997-04-24
EP0856059A1 (en) 1998-08-05
AU7208896A (en) 1997-05-07

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