DE69629576D1 - Verfahren zur herstellung von rekombinanten plasmiden - Google Patents

Verfahren zur herstellung von rekombinanten plasmiden

Info

Publication number
DE69629576D1
DE69629576D1 DE69629576T DE69629576T DE69629576D1 DE 69629576 D1 DE69629576 D1 DE 69629576D1 DE 69629576 T DE69629576 T DE 69629576T DE 69629576 T DE69629576 T DE 69629576T DE 69629576 D1 DE69629576 D1 DE 69629576D1
Authority
DE
Germany
Prior art keywords
cells
genetic material
culture system
toxic
plasmids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69629576T
Other languages
English (en)
Other versions
DE69629576T2 (de
Inventor
A Morsey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Application granted granted Critical
Publication of DE69629576D1 publication Critical patent/DE69629576D1/de
Publication of DE69629576T2 publication Critical patent/DE69629576T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
DE69629576T 1995-10-17 1996-10-17 Verfahren zur herstellung von rekombinanten plasmiden Expired - Fee Related DE69629576T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US54805995A 1995-10-17 1995-10-17
US548059 1995-10-17
US56497395A 1995-11-30 1995-11-30
US564973 1995-11-30
PCT/CA1996/000693 WO1997014805A2 (en) 1995-10-17 1996-10-17 Methods for production of recombinant plasmids

Publications (2)

Publication Number Publication Date
DE69629576D1 true DE69629576D1 (de) 2003-09-25
DE69629576T2 DE69629576T2 (de) 2004-06-24

Family

ID=27068752

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69629576T Expired - Fee Related DE69629576T2 (de) 1995-10-17 1996-10-17 Verfahren zur herstellung von rekombinanten plasmiden

Country Status (9)

Country Link
US (1) US5922583A (de)
EP (1) EP0856059B1 (de)
JP (1) JPH11513562A (de)
AT (1) ATE247713T1 (de)
AU (1) AU7208896A (de)
CA (1) CA2232234A1 (de)
DE (1) DE69629576T2 (de)
TW (1) TW514667B (de)
WO (1) WO1997014805A2 (de)

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US6413768B1 (en) 1998-12-02 2002-07-02 University Of Maryland Expression plasmids
US6423545B1 (en) * 1999-07-08 2002-07-23 Albert Einstein College Of Medicine Of Yeshiva University Unmarked deletion mutants of mycobacteria and methods of using same
EP1111061A1 (de) * 1999-12-20 2001-06-27 Universite Libre De Bruxelles Vektor mit doppelter Selektion
US9012141B2 (en) * 2000-03-27 2015-04-21 Advaxis, Inc. Compositions and methods comprising KLK3 of FOLH1 antigen
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US7396822B2 (en) 2001-05-24 2008-07-08 Vaxiion Therapeutics, Inc. Immunogenic minicells and methods of use
US20030194798A1 (en) 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
US20030224444A1 (en) * 2002-02-25 2003-12-04 Sabbadini Roger A. Antibodies to native conformations of membrane proteins
US20030207833A1 (en) * 2002-02-25 2003-11-06 Neil Berkley Pharmaceutical compositions with minicells
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CA2477194A1 (en) * 2002-03-19 2003-09-25 Universite Libre De Bruxelles Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms
US20040005700A1 (en) * 2002-05-28 2004-01-08 Surber Mark W. Poroplasts
US9309518B2 (en) 2002-09-03 2016-04-12 Universite Libre De Bruxelles Reversible, parallel and multitask cloning method and kit
ATE383421T1 (de) * 2002-09-03 2008-01-15 Univ Bruxelles Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
US20060002956A1 (en) * 2004-04-05 2006-01-05 Surber Mark W Minicells as vaccines
ES2543079T3 (es) 2004-08-13 2015-08-14 The Trustees Of The University Of Pennsylvania Métodos para construir vacunas sin resistencia antibiótica
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CN1912127B (zh) * 2005-08-08 2011-05-25 百瑞全球有限公司 一种大肠杆菌表达载体及其应用
US9109012B2 (en) 2007-05-29 2015-08-18 Nature Technology Corporation Vectors and method for genetic immunization
US9084747B2 (en) * 2009-11-11 2015-07-21 Advaxis, Inc. Compositions and methods for prevention of escape mutation in the treatment of HER2/NEU over-expressing tumors
US9650639B2 (en) 2008-05-19 2017-05-16 Advaxis, Inc. Dual delivery system for heterologous antigens
US9017660B2 (en) 2009-11-11 2015-04-28 Advaxis, Inc. Compositions and methods for prevention of escape mutation in the treatment of Her2/neu over-expressing tumors
US20120135033A1 (en) * 2008-05-19 2012-05-31 Anu Wallecha Multiple delivery system for heterologous antigens
EP2288379A4 (de) 2008-05-19 2012-08-08 Advaxis Doppeltes freisetzungssystem für heterologe antigene
US10016617B2 (en) 2009-11-11 2018-07-10 The Trustees Of The University Of Pennsylvania Combination immuno therapy and radiotherapy for the treatment of Her-2-positive cancers
JP5981436B2 (ja) 2010-10-01 2016-08-31 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア 寄生的に感染している対象におけるワクチン不応答性を反転させるためのリステリアワクチンベクターの使用
JP6199746B2 (ja) 2011-02-15 2017-09-20 バキシオン セラピューティクス，リミテッド ライアビリティ カンパニー 抗体およびＦｃ含有標的化分子に基づく生理活性分子の標的送達のための細菌ミニ細胞による治療用組成物および方法
CA2829960A1 (en) 2011-03-11 2012-09-20 John Rothman Listeria-based adjuvants
BR112014022662A2 (pt) 2012-03-12 2017-10-03 Advaxis Inc Inibição da função de célula supressora seguindo tratamento de vacina de listeria
CA2883227A1 (en) 2012-08-29 2014-03-06 Nature Technology Corporation Dna plasmids with improved expression
US20150322439A1 (en) 2012-11-19 2015-11-12 Nature Technology Corporation Replicative minicircle vectors with improved expression
BR122021015417B1 (pt) 2013-08-19 2022-11-08 Syngulon Sa Célula microbiana geneticamente engenheirada que compreende um ácido nucleico que codifica uma bacteriocina
ES2821655T3 (es) 2017-09-19 2021-04-27 Deutsches Krebsforsch Vectores de ADN no integrantes para la modificación genética de células
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
US11932672B2 (en) 2017-12-19 2024-03-19 Syngulon S.A. Fermentation process
JP2021518150A (ja) 2018-03-21 2021-08-02 ネイチャー テクノロジー コーポレーション 生成が改良されたウイルス及び非ウイルスのナノプラスミドベクター

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Also Published As

Publication number Publication date
WO1997014805A2 (en) 1997-04-24
CA2232234A1 (en) 1997-04-24
DE69629576T2 (de) 2004-06-24
AU7208896A (en) 1997-05-07
EP0856059A1 (de) 1998-08-05
EP0856059B1 (de) 2003-08-20
US5922583A (en) 1999-07-13
TW514667B (en) 2002-12-21
JPH11513562A (ja) 1999-11-24
ATE247713T1 (de) 2003-09-15
WO1997014805A3 (en) 1997-06-12

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Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee