ATE383421T1 - Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien - Google Patents

Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien

Info

Publication number
ATE383421T1
ATE383421T1 AT03793509T AT03793509T ATE383421T1 AT E383421 T1 ATE383421 T1 AT E383421T1 AT 03793509 T AT03793509 T AT 03793509T AT 03793509 T AT03793509 T AT 03793509T AT E383421 T1 ATE383421 T1 AT E383421T1
Authority
AT
Austria
Prior art keywords
reversible
parallel
cloning method
multitask
corresponding reagents
Prior art date
Application number
AT03793509T
Other languages
English (en)
Inventor
Cedric Szpirer
Michel Milinkovitch
Philippe Gabant
Original Assignee
Univ Bruxelles
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Bruxelles filed Critical Univ Bruxelles
Application granted granted Critical
Publication of ATE383421T1 publication Critical patent/ATE383421T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03793509T 2002-09-03 2003-09-03 Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien ATE383421T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US40848202P 2002-09-03 2002-09-03

Publications (1)

Publication Number Publication Date
ATE383421T1 true ATE383421T1 (de) 2008-01-15

Family

ID=31978622

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03793509T ATE383421T1 (de) 2002-09-03 2003-09-03 Reversible, parallele und zur vielfältigen aufgabenbewältigung verwendbare (multitask) klonierungsmethode und entprechende reagentien

Country Status (9)

Country Link
US (1) US8318497B2 (de)
EP (1) EP1556485B1 (de)
JP (1) JP4546828B2 (de)
CN (1) CN1703509B (de)
AT (1) ATE383421T1 (de)
AU (1) AU2003260200A1 (de)
CA (1) CA2496514C (de)
DE (1) DE60318603T2 (de)
WO (1) WO2004022745A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT395483B (de) * 1989-01-13 1993-01-25 Vaillant Gmbh Rein elektrisch beheizter durchlauferhitzer mit vorrichtung zum ermitteln des wasserdurchsatzes

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU766257C (en) 1998-05-07 2004-06-03 Universite Libre De Bruxelles Cytotoxin-based biological containment
CA2435956C (en) 2001-02-23 2012-07-10 Universite Libre De Bruxelles Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule
EP2119789A1 (de) 2008-05-16 2009-11-18 Université Libre de Bruxelles Hyperproliferative rekombinante Zelle
EP2612918A1 (de) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta-Rekombination
US9333227B2 (en) 2013-08-19 2016-05-10 Syngulon Sa. Controlled growth of microorganisms
US10696997B2 (en) 2014-07-04 2020-06-30 Universite Libre De Bruxelles Method and system for the production of recombinant proteins by cells
WO2016012607A1 (en) * 2014-07-25 2016-01-28 Delphi Genetics Improved host cell for producing proteins
WO2019121983A1 (en) 2017-12-19 2019-06-27 Syngulon S.A. Fermentation process

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3750125T2 (de) 1986-03-26 1994-10-27 Genexpress Aps Biologische eindämmung.
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5300431A (en) 1991-02-26 1994-04-05 E. I. Du Pont De Nemours And Company Positive selection vector for the bacteriophage P1 cloning system
US7176029B2 (en) 1992-07-31 2007-02-13 Universite Libre De Bruxelles Cloning and/or sequencing vector
BE1006085A3 (fr) 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
JP2590761B2 (ja) 1994-11-22 1997-03-12 日本電気株式会社 Tab式半導体装置およびtab式半導体装置を回路基板に接続する方法
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
DE69623057T2 (de) 1995-06-07 2003-03-27 Invitrogen Corp., Carlsbad Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
WO1997013401A1 (en) 1995-10-13 1997-04-17 Purdue Research Foundation Method for the production of hybrid plants
US5922583A (en) 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
ATE341621T1 (de) 1997-10-24 2006-10-15 Invitrogen Corp Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
AU766257C (en) 1998-05-07 2004-06-03 Universite Libre De Bruxelles Cytotoxin-based biological containment
US6134557A (en) * 1998-11-20 2000-10-17 Matlink, Inc. Materials and supplies ordering system
US6271359B1 (en) 1999-04-14 2001-08-07 Musc Foundation For Research Development Tissue-specific and pathogen-specific toxic agents and ribozymes
NZ529476A (en) 1999-10-25 2005-03-24 Invitrogen Corp Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
CN1757724B (zh) 1999-12-10 2014-06-11 茵维特罗根公司 具有独特特异性的多个重组位点在重组克隆中的用途
EP1111061A1 (de) * 1999-12-20 2001-06-27 Universite Libre De Bruxelles Vektor mit doppelter Selektion
DE10038573A1 (de) 2000-08-03 2002-02-21 Mpb Cologne Gmbh Molecular Pla Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen
CA2435956C (en) 2001-02-23 2012-07-10 Universite Libre De Bruxelles Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule
US6531153B2 (en) * 2001-05-29 2003-03-11 Drugtech Corporation Composition with sustained release of levodopa and carbidopa
EP1485491A1 (de) 2002-03-19 2004-12-15 Universite Libre De Bruxelles Toxin/antitoxin-system zur selektion genetisch modifizierter eukaryontischer zellen oder organismen

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT395483B (de) * 1989-01-13 1993-01-25 Vaillant Gmbh Rein elektrisch beheizter durchlauferhitzer mit vorrichtung zum ermitteln des wasserdurchsatzes

Also Published As

Publication number Publication date
AU2003260200A8 (en) 2004-03-29
JP2005537016A (ja) 2005-12-08
US8318497B2 (en) 2012-11-27
CN1703509A (zh) 2005-11-30
CN1703509B (zh) 2010-09-01
WO2004022745A3 (en) 2005-06-02
WO2004022745A2 (en) 2004-03-18
CA2496514C (en) 2012-11-06
CA2496514A1 (en) 2004-03-18
DE60318603T2 (de) 2008-07-03
EP1556485B1 (de) 2008-01-09
AU2003260200A1 (en) 2004-03-29
JP4546828B2 (ja) 2010-09-22
EP1556485A2 (de) 2005-07-27
US20060088841A1 (en) 2006-04-27
DE60318603D1 (de) 2008-02-21

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