RU94034733A - Способ получения клонирования и экспрессии гена, гены, иммунотоксины, продукты генов, днк, клетка, способ проверки гена и способ коррекции мутаций - Google Patents

Способ получения клонирования и экспрессии гена, гены, иммунотоксины, продукты генов, днк, клетка, способ проверки гена и способ коррекции мутаций

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Publication number
RU94034733A
RU94034733A RU94034733/13A RU94034733A RU94034733A RU 94034733 A RU94034733 A RU 94034733A RU 94034733/13 A RU94034733/13 A RU 94034733/13A RU 94034733 A RU94034733 A RU 94034733A RU 94034733 A RU94034733 A RU 94034733A
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RU
Russia
Prior art keywords
gene
cloning
expression
synthetic gene
gelonine
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RU94034733/13A
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English (en)
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RU2142015C1 (ru
Inventor
Г. Розенблюм Майкл
Us]
Л.Битти Кеннет
Original Assignee
Рисерч Дивелопмент Фаундейшн (US)
Рисерч Дивелопмент Фаундейшн
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Application filed by Рисерч Дивелопмент Фаундейшн (US), Рисерч Дивелопмент Фаундейшн filed Critical Рисерч Дивелопмент Фаундейшн (US)
Publication of RU94034733A publication Critical patent/RU94034733A/ru
Application granted granted Critical
Publication of RU2142015C1 publication Critical patent/RU2142015C1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
    • A61K47/6817—Toxins
    • A61K47/6819—Plant toxins
    • A61K47/6825—Ribosomal inhibitory proteins, i.e. RIP-I or RIP-II, e.g. Pap, gelonin or dianthin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Изобретение раскрывает нуклеотидную последовательность синтетического гена для растительного токсина гелонина и способ получения, клонирования и экспрессии этого синтетического гена. ДНК последовательность для синтетического гена для гелонина представлена в последовательности ID N 1. В настоящем изобретении предложены также векторы экспрессии, содержащие ДНК последовательности для гелонина, и клетки, траснформированные этими векторами. Кроме того, предложен иммунотоксин, содержащий антитела, коньюгированные с протеином гелонином.

Claims (1)

  1. Изобретение раскрывает нуклеотидную последовательность синтетического гена для растительного токсина гелонина и способ получения, клонирования и экспрессии этого синтетического гена. ДНК последовательность для синтетического гена для гелонина представлена в последовательности ID N 1. В настоящем изобретении предложены также векторы экспрессии, содержащие ДНК последовательности для гелонина, и клетки, траснформированные этими векторами. Кроме того, предложен иммунотоксин, содержащий антитела, коньюгированные с протеином гелонином.
RU94034733A 1991-09-06 1992-08-21 Фрагмент днк, кодирующий белок гелонина, и способ его получения, вектор, обеспечивающий экспрессию белка гелонина, рекомбинантный штамм e.coli - продуцент белка гелонина RU2142015C1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US75594991A 1991-09-06 1991-09-06
US07/755,949 1991-09-06
PCT/US1992/007066 WO1993005168A1 (en) 1991-09-06 1992-08-21 Dna sequences encoding gelonin polypeptide

Publications (2)

Publication Number Publication Date
RU94034733A true RU94034733A (ru) 1996-06-10
RU2142015C1 RU2142015C1 (ru) 1999-11-27

Family

ID=25041367

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94034733A RU2142015C1 (ru) 1991-09-06 1992-08-21 Фрагмент днк, кодирующий белок гелонина, и способ его получения, вектор, обеспечивающий экспрессию белка гелонина, рекомбинантный штамм e.coli - продуцент белка гелонина

Country Status (18)

Country Link
US (1) US5624827A (ru)
EP (1) EP0602144B1 (ru)
JP (1) JPH06510431A (ru)
KR (1) KR100270195B1 (ru)
CN (1) CN1047402C (ru)
AT (1) ATE173023T1 (ru)
AU (1) AU679427B2 (ru)
CA (1) CA2115049C (ru)
DE (1) DE69227519T2 (ru)
DK (1) DK0602144T3 (ru)
ES (1) ES2123008T3 (ru)
FI (1) FI113547B (ru)
IL (1) IL103057A (ru)
NO (1) NO317659B1 (ru)
NZ (1) NZ244220A (ru)
RU (1) RU2142015C1 (ru)
WO (1) WO1993005168A1 (ru)
ZA (1) ZA926705B (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2571218C2 (ru) * 2009-05-14 2015-12-20 Байонир Корпорейшн Конъюгат мирнк и способ его получения

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US5317009A (en) * 1991-08-26 1994-05-31 New York University Anti-HIV proteins GAP 31, DAP 30 and DAP 32 and therapeutic uses thereof
US6146850A (en) * 1991-11-04 2000-11-14 Xoma Corporation Proteins encoding gelonin sequences
US5621083A (en) * 1991-11-04 1997-04-15 Xoma Corporation Immunotoxins comprising ribosome-inactivating proteins
US5837491A (en) * 1991-11-04 1998-11-17 Xoma Corporation Polynucleotides encoding gelonin sequences
US5869619A (en) * 1991-12-13 1999-02-09 Xoma Corporation Modified antibody variable domains
CA2103887C (en) * 1991-12-13 2005-08-30 Gary M. Studnicka Methods and materials for preparation of modified antibody variable domains and therapeutic uses thereof
JPH08510642A (ja) * 1993-05-12 1996-11-12 ゾマ コーポレイション ゲロニンおよび抗体から成る免疫毒素
US20030166169A1 (en) * 1998-01-16 2003-09-04 Padgett Hal S. Method for constructing viral nucleic acids in a cell-free manner
ZA200305980B (en) * 2001-02-12 2007-01-31 Res Dev Foundation Modified proteins, designer toxins, and methods of making thereof
EP1414471B1 (en) * 2001-07-17 2012-06-13 Research Development Foundation Therapeutic agents comprising pro-apoptotic proteins
WO2003105761A2 (en) * 2002-06-12 2003-12-24 Research Development Foundation Immunotoxin as a therapeutic agent and uses thereof
NZ563328A (en) 2002-10-16 2009-08-28 Euro Celtique Sa Antibodies that bind cell-associated CA 125/0772P and methods of use thereof
CN101132813A (zh) * 2005-02-01 2008-02-27 研究发展基金会 靶向blys受体的blys融合蛋白和用于治疗b细胞增殖性疾病的方法
US8759036B2 (en) * 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
US20130109596A1 (en) 2011-09-26 2013-05-02 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2014055836A2 (en) 2012-10-04 2014-04-10 Research Development Foundation Serine protease molecules and therapies
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
US20180291413A1 (en) 2015-10-06 2018-10-11 Thermo Fisher Scientific Geneart Gmbh Devices and methods for producing nucleic acids and proteins
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
JP2023516827A (ja) 2020-03-06 2023-04-20 ライフ テクノロジーズ コーポレイション 高配列忠実度の核酸合成およびアセンブリ

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Publication number Priority date Publication date Assignee Title
US4888415A (en) * 1985-03-04 1989-12-19 Dana-Farber Cancer Institute, Inc. Gelonin immunotoxin
US5024947A (en) * 1987-07-24 1991-06-18 Cetus Corporation Serum free media for the growth on insect cells and expression of products thereby
US5290914A (en) * 1988-04-28 1994-03-01 Mycogen Corporation Hybrid diphtheria-B.t. pesticidal toxins
IE912716A1 (en) * 1990-08-14 1992-02-26 Res Dev Foundation Protein Structure of the Plant Toxin Gelonin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2571218C2 (ru) * 2009-05-14 2015-12-20 Байонир Корпорейшн Конъюгат мирнк и способ его получения

Also Published As

Publication number Publication date
EP0602144B1 (en) 1998-11-04
RU2142015C1 (ru) 1999-11-27
DE69227519T2 (de) 1999-06-02
IL103057A (en) 1999-09-22
FI941048A0 (fi) 1994-03-04
AU2547692A (en) 1993-04-05
NO317659B1 (no) 2004-11-29
CA2115049A1 (en) 1993-03-18
AU679427B2 (en) 1997-07-03
ZA926705B (en) 1993-04-28
CA2115049C (en) 2003-10-21
WO1993005168A1 (en) 1993-03-18
DE69227519D1 (de) 1998-12-10
JPH06510431A (ja) 1994-11-24
NO940736D0 (no) 1994-03-03
NZ244220A (en) 1994-12-22
FI113547B (fi) 2004-05-14
CN1047402C (zh) 1999-12-15
FI941048A (fi) 1994-03-04
NO940736L (no) 1994-03-03
EP0602144A1 (en) 1994-06-22
DK0602144T3 (da) 1999-07-19
US5624827A (en) 1997-04-29
CN1070227A (zh) 1993-03-24
ES2123008T3 (es) 1999-01-01
ATE173023T1 (de) 1998-11-15
EP0602144A4 (en) 1995-03-15
IL103057A0 (en) 1993-02-21
KR100270195B1 (ko) 2001-03-02

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Effective date: 20100822