AU5049100A - Phage-dependent super-production of biologically active protein and peptides - Google Patents
Phage-dependent super-production of biologically active protein and peptidesInfo
- Publication number
- AU5049100A AU5049100A AU50491/00A AU5049100A AU5049100A AU 5049100 A AU5049100 A AU 5049100A AU 50491/00 A AU50491/00 A AU 50491/00A AU 5049100 A AU5049100 A AU 5049100A AU 5049100 A AU5049100 A AU 5049100A
- Authority
- AU
- Australia
- Prior art keywords
- phage
- production
- peptides
- biologically active
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 3
- 230000001419 dependent effect Effects 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 241000701959 Escherichia virus Lambda Species 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000002101 lytic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/73—Expression systems using phage (lambda) regulatory sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10111—Myoviridae
- C12N2795/10141—Use of virus, viral particle or viral elements as a vector
- C12N2795/10143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
This invention relates to a method for enhancing the production of biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage lambda with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/318,288 US6268178B1 (en) | 1999-05-25 | 1999-05-25 | Phage-dependent super-production of biologically active protein and peptides |
US09318288 | 1999-05-25 | ||
PCT/US2000/040020 WO2000071731A1 (en) | 1999-05-25 | 2000-05-24 | Phage-dependent super-production of biologically active protein and peptides |
Publications (1)
Publication Number | Publication Date |
---|---|
AU5049100A true AU5049100A (en) | 2000-12-12 |
Family
ID=23237514
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU50491/00A Abandoned AU5049100A (en) | 1999-05-25 | 2000-05-24 | Phage-dependent super-production of biologically active protein and peptides |
Country Status (7)
Country | Link |
---|---|
US (2) | US6268178B1 (en) |
EP (1) | EP1180153B1 (en) |
JP (1) | JP2003500059A (en) |
AT (1) | ATE293170T1 (en) |
AU (1) | AU5049100A (en) |
DE (1) | DE60019411T2 (en) |
WO (1) | WO2000071731A1 (en) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7252818B2 (en) * | 1998-07-24 | 2007-08-07 | Cardiovascular Biotherapeutics, Inc. | Method of producing biologically active human acidic fibroblast growth factor and its use in promoting angiogenesis |
US6268178B1 (en) * | 1999-05-25 | 2001-07-31 | Phage Biotechnology Corp. | Phage-dependent super-production of biologically active protein and peptides |
KR100761486B1 (en) * | 2000-08-15 | 2007-10-04 | 카디오배스큘러 바이오 떼러퓨틱스, 인크. | Phage-Dependent Superproduction of Biologically Active Protein and Peptides |
CA2419338A1 (en) * | 2000-08-15 | 2002-02-21 | Phage Biotechnology Corporation | A method of producing biologically active human acidic fibroblast growth factor and its use in promoting angiogenesis |
KR20050071498A (en) * | 2002-09-18 | 2005-07-07 | 상트르 오스피딸리에 드 루니버시떼 드 몬트리알 | Ghrh analogues |
WO2019068006A1 (en) * | 2017-09-29 | 2019-04-04 | The Charles Stark Draper Laboratory, Inc. | Stimulus-controlled cell lysis |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE276694C (en) | ||||
FI86437C (en) | 1978-12-22 | 1992-08-25 | Biogen Inc | A FRUIT PROCEDURE FOR THE PREPARATION OF A POLYPEPTIDIDE WITH ANTIGENA EGENSKAPERNA HOS ETT HEPATITIS B- VIRUSANTIGEN. |
CH651308A5 (en) | 1980-07-01 | 1985-09-13 | Hoffmann La Roche | INTERFERON AND THEIR PRODUCTION. |
US4775622A (en) | 1982-03-08 | 1988-10-04 | Genentech, Inc. | Expression, processing and secretion of heterologous protein by yeast |
CA1209501A (en) | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
JPS5979746A (en) | 1982-10-30 | 1984-05-09 | Mitsubishi Heavy Ind Ltd | Control apparatus for vulcanizing machine |
SU1089119A1 (en) * | 1982-11-29 | 1984-04-30 | Институт Молекулярной Биологии И Генетики Ан Усср | Process for preparing beta-lactamase |
GB2143238A (en) | 1983-03-31 | 1985-02-06 | Dr Anthony Stuart Breeze | A method for enzyme liberation from bacterial cells |
US4637980A (en) * | 1983-08-09 | 1987-01-20 | Smithkline Beckman Corporation | Externalization of products of bacteria |
CA1309044C (en) | 1987-11-09 | 1992-10-20 | Toshiya Takano | Method of producing foreign gene products |
IL92124A (en) | 1988-10-28 | 1996-10-31 | Sidney Pestka | Recombinant proteins modified to contain non-naturally occurring phosphorylation sites |
US5102797A (en) | 1989-05-26 | 1992-04-07 | Dna Plant Technology Corporation | Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system |
US5196318A (en) | 1990-06-26 | 1993-03-23 | The Texas A&M University System | Precisely regulated expression of deleterious genes |
NZ314630A (en) | 1991-01-17 | 2000-11-24 | Harvard College | Use of trans-splicing ribozymes for genetic modification and cell ablation in a host cell |
US5346830A (en) | 1991-06-11 | 1994-09-13 | The Center For Innovative Technology | Gene expression system based on regulatory elements of bateriophage P2 and satellite phage P4 |
US5834233A (en) | 1992-04-06 | 1998-11-10 | Gx Biosystems A/S | Method of limiting the survival of genetically engineered microorganisms in their enivronment |
US5622852A (en) | 1994-10-31 | 1997-04-22 | Washington University | Bcl-x/Bcl-2 associated cell death regulator |
US5861273A (en) | 1993-12-21 | 1999-01-19 | Celtrix Phamraceuticals, Inc. | Chromosomal expression of heterologous genes in bacterial cells |
US6268178B1 (en) * | 1999-05-25 | 2001-07-31 | Phage Biotechnology Corp. | Phage-dependent super-production of biologically active protein and peptides |
KR100761486B1 (en) * | 2000-08-15 | 2007-10-04 | 카디오배스큘러 바이오 떼러퓨틱스, 인크. | Phage-Dependent Superproduction of Biologically Active Protein and Peptides |
CA2419338A1 (en) * | 2000-08-15 | 2002-02-21 | Phage Biotechnology Corporation | A method of producing biologically active human acidic fibroblast growth factor and its use in promoting angiogenesis |
-
1999
- 1999-05-25 US US09/318,288 patent/US6268178B1/en not_active Expired - Fee Related
-
2000
- 2000-05-24 AU AU50491/00A patent/AU5049100A/en not_active Abandoned
- 2000-05-24 JP JP2000620108A patent/JP2003500059A/en active Pending
- 2000-05-24 DE DE60019411T patent/DE60019411T2/en not_active Expired - Lifetime
- 2000-05-24 EP EP00932819A patent/EP1180153B1/en not_active Expired - Lifetime
- 2000-05-24 AT AT00932819T patent/ATE293170T1/en active
- 2000-05-24 WO PCT/US2000/040020 patent/WO2000071731A1/en active IP Right Grant
-
2001
- 2001-05-17 US US09/859,651 patent/US6794162B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP1180153A1 (en) | 2002-02-20 |
US6268178B1 (en) | 2001-07-31 |
DE60019411D1 (en) | 2005-05-19 |
US20010044133A1 (en) | 2001-11-22 |
ATE293170T1 (en) | 2005-04-15 |
DE60019411T2 (en) | 2006-03-09 |
US6794162B2 (en) | 2004-09-21 |
JP2003500059A (en) | 2003-01-07 |
EP1180153B1 (en) | 2005-04-13 |
WO2000071731A1 (en) | 2000-11-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
MK6 | Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase |