KR920012446A - 병원체-저항식물의 제조방법 - Google Patents

병원체-저항식물의 제조방법 Download PDF

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Publication number
KR920012446A
KR920012446A KR1019910023698A KR910023698A KR920012446A KR 920012446 A KR920012446 A KR 920012446A KR 1019910023698 A KR1019910023698 A KR 1019910023698A KR 910023698 A KR910023698 A KR 910023698A KR 920012446 A KR920012446 A KR 920012446A
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South Korea
Prior art keywords
protein
synthesis inhibitor
pathogen
plant
inhibitor gene
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KR1019910023698A
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English (en)
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쉘 예프
로게만 위르겐
야크 귀도
먼디 존
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원본미기재
막스-플랑크-게젤샤프트 쭈어 페르데퉁 데어 비센샤프텐 에.파우
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Publication of KR920012446A publication Critical patent/KR920012446A/ko

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01014—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01039—Glucan endo-1,3-beta-D-glucosidase (3.2.1.39)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
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  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Cultivation Of Plants (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)

Abstract

내용 없음

Description

병원체-저항식물의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 보리로부터의 CHI, BGL 및 PSI 단백질 정제의 SDS-겔-전기영동사진, 제2도는 정제 단백질을 사용한 곰팡이의 생욱 테스트 결과를 도시한 도면.

Claims (8)

  1. 단백질-합성 저해제 유전자나 또는 세포에의 특이적 결합을 가능케하는 리칸드와 단백질-합성 저해제 유전자와의 융합 생성물 또는 단백질-합성 저해제 단백질과의 융합 생성물을 활성 프로모터의 통제하에 식물의 유전자형내로 도입하는 것으로 됨을 특징으로 하는 병원체-저항식물의 제조방법.
  2. 제1항에 있어서, 식물로 부터 분리된 단백질-합성 저해제 유전자를 단백질-합성 저해제 유전자로서 사용하는 것이 특징인 방법.
  3. 제2항에 있어서, 보리식물로부터 분리되고 다음의 DNA배열을 갖는 단백질-합성 저해제 유전자를 사용하는 것이 특징인 방법.
  4. 제3항에 기재된 DNA배열이 삽입된 DNA전달벡터.
  5. 제3항에 기재된 DNA배열이 삽입된 DNA발현벡터.
  6. 제1항, 2항 또는 3항의 방법에 의해 변형된 유전자형을 갖는 식물 또는 식물부분.
  7. 세균 오버프로듀서내의 단백질-합성 저해제 유전자를 도입함으로써, 얻어지는 단백질-합성 저해제 단백질의 병원체 내성 획득, 병원체 감염퇴치 및/또는 퇴행성 세포퇴치를 위한 약리적 제제생산 용도.
  8. 제7항에 있어서, 주입용액 형태의 단백질-합성 저해제 단백질의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910023698A 1990-12-20 1991-12-20 병원체-저항식물의 제조방법 KR920012446A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4040954.6 1990-12-20
DE4040954A DE4040954C2 (de) 1990-12-20 1990-12-20 Verfahren zur Erzeugung von pathogen-resistenten Pflanzen

Publications (1)

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KR920012446A true KR920012446A (ko) 1992-07-27

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Application Number Title Priority Date Filing Date
KR1019910023698A KR920012446A (ko) 1990-12-20 1991-12-20 병원체-저항식물의 제조방법

Country Status (13)

Country Link
US (2) US5633442A (ko)
EP (2) EP0492536B1 (ko)
JP (1) JPH06113856A (ko)
KR (1) KR920012446A (ko)
AT (1) ATE240400T1 (ko)
AU (1) AU657929B2 (ko)
CA (1) CA2057313C (ko)
DE (2) DE4040954C2 (ko)
ES (1) ES2198401T3 (ko)
HU (1) HU217421B (ko)
IE (1) IE914447A1 (ko)
IL (1) IL100244A0 (ko)
ZA (1) ZA919630B (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5635384A (en) * 1990-06-11 1997-06-03 Dowelanco Ribosome-inactivating proteins, inactive precursor forms thereof, a process for making and a method of using
DE4040954C2 (de) * 1990-12-20 2001-05-17 Max Planck Gesellschaft Verfahren zur Erzeugung von pathogen-resistenten Pflanzen
USRE39238E1 (en) 1992-10-09 2006-08-15 Max-Planck-Gesellschaft zür Forderung der Wissenschaften e.V. Transgenic pathogen-resistant organism
DE4234131C2 (de) * 1992-10-09 1995-08-24 Max Planck Gesellschaft Transgener pathogen-resistenter Organismus
DE4442179C2 (de) * 1994-11-26 1996-12-12 Inst Pflanzengenetik & Kultur Pathogenresistente Pflanzen und Verfahren zu ihrer Herstellung
GB9507381D0 (en) * 1995-04-10 1995-05-31 Zeneca Ltd S-adenosyl-l-homocysteine hydrolase
US8716001B2 (en) 2009-02-06 2014-05-06 Cornell University Trichoderma strains that induce resistance to plant diseases and/or increase plant growth

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0298918B1 (de) * 1987-07-10 2001-09-05 Syngenta Participations AG Induzierbare Virusresistenz bei Pflanzen
WO1989004371A1 (en) * 1987-11-02 1989-05-18 Louisiana State University Agricultural And Mechan Plants genetically enhanced for disease resistance
GB8801877D0 (en) * 1988-01-28 1988-02-24 Erba Carlo Spa Nucleotide sequence encoding plant ribosome inactivating protein
IE890206L (en) * 1988-02-02 1989-08-02 Akzo Nv Immunotoxins from barley toxin
CN1033645A (zh) * 1988-10-22 1989-07-05 中国科学院上海植物生理研究所 控制植物病毒病的基因工程方法
DE3843628A1 (de) * 1988-12-21 1990-07-05 Inst Genbiologische Forschung Wundinduzierbare und kartoffelknollenspezifische transkriptionale regulation
KR920701438A (ko) * 1989-04-04 1992-08-11 원본미기재 재조합 트리코산틴 및 코딩 서열
WO1990011770A1 (en) * 1989-04-11 1990-10-18 Calgene, Inc. Use of animal-derived anti-microbial peptides for control of plant pathogens
US5126324A (en) * 1990-06-07 1992-06-30 Genentech, Inc. Method of enhancing growth in patients using combination therapy
US5248606A (en) * 1990-06-11 1993-09-28 Dowelanco Dna encoding inactive precursor and active forms of maize ribosome inactivating protein
DE4040954C2 (de) * 1990-12-20 2001-05-17 Max Planck Gesellschaft Verfahren zur Erzeugung von pathogen-resistenten Pflanzen
US5376546A (en) * 1991-11-04 1994-12-27 Xoma Corporation Analogs of ribosome-inactivating proteins

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Publication number Publication date
EP0492536B1 (de) 2003-05-14
ES2198401T3 (es) 2004-02-01
IE914447A1 (en) 1992-07-01
CA2057313A1 (en) 1992-06-21
HU217421B (hu) 2000-01-28
AU8971691A (en) 1992-06-25
AU657929B2 (en) 1995-03-30
US5633442A (en) 1997-05-27
EP0492536A2 (de) 1992-07-01
HUT60779A (en) 1992-10-28
ZA919630B (en) 1992-09-30
JPH06113856A (ja) 1994-04-26
HU914035D0 (en) 1992-03-30
EP0896059A2 (de) 1999-02-10
EP0492536A3 (en) 1992-08-05
US6271442B1 (en) 2001-08-07
DE4040954C2 (de) 2001-05-17
IL100244A0 (en) 1992-09-06
EP0896059A3 (de) 1999-07-07
DE4040954A1 (de) 1992-06-25
CA2057313C (en) 2003-02-04
DE59109251D1 (de) 2003-06-18
ATE240400T1 (de) 2003-05-15

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