DE69533317D1 - Isolierung von nukleinsäuren - Google Patents

Isolierung von nukleinsäuren

Info

Publication number
DE69533317D1
DE69533317D1 DE69533317T DE69533317T DE69533317D1 DE 69533317 D1 DE69533317 D1 DE 69533317D1 DE 69533317 T DE69533317 T DE 69533317T DE 69533317 T DE69533317 T DE 69533317T DE 69533317 D1 DE69533317 D1 DE 69533317D1
Authority
DE
Germany
Prior art keywords
sample
nucleic acid
isolation
nucleic acids
support
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69533317T
Other languages
English (en)
Other versions
DE69533317T2 (de
Inventor
Arne Helge Deggerdal
Frank Larsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Dynal AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10765877&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69533317(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dynal AS filed Critical Dynal AS
Application granted granted Critical
Publication of DE69533317D1 publication Critical patent/DE69533317D1/de
Publication of DE69533317T2 publication Critical patent/DE69533317T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE69533317T 1994-12-12 1995-12-12 Isolierung von nukleinsäuren Expired - Lifetime DE69533317T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9425138.6A GB9425138D0 (en) 1994-12-12 1994-12-12 Isolation of nucleic acid
GB9425138 1994-12-12
PCT/GB1995/002893 WO1996018731A2 (en) 1994-12-12 1995-12-12 Isolation of nucleic acid

Publications (2)

Publication Number Publication Date
DE69533317D1 true DE69533317D1 (de) 2004-09-02
DE69533317T2 DE69533317T2 (de) 2005-12-01

Family

ID=10765877

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69533317T Expired - Lifetime DE69533317T2 (de) 1994-12-12 1995-12-12 Isolierung von nukleinsäuren

Country Status (9)

Country Link
US (8) US20040215011A1 (de)
EP (1) EP0796327B1 (de)
JP (1) JP3787354B2 (de)
AT (1) ATE272110T1 (de)
AU (1) AU706211B2 (de)
CA (1) CA2207608C (de)
DE (1) DE69533317T2 (de)
GB (1) GB9425138D0 (de)
WO (1) WO1996018731A2 (de)

Families Citing this family (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
DE69734263T2 (de) * 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
WO1999013976A1 (en) 1997-09-17 1999-03-25 Gentra Systems, Inc. Apparatuses and methods for isolating nucleic acid
CA2319665C (en) * 1998-02-02 2010-06-29 Gentra Systems, Inc. Processes for isolating, amplifying and characterizing dna
US7670768B1 (en) * 1998-02-02 2010-03-02 Qiagen North American Holdings, Inc. Processes for isolating, amplifying and characterizing DNA
AU2574499A (en) * 1998-02-02 1999-08-16 Gentra Systems, Inc. Compositions and methods for using a lysing matrix for isolating dna
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
WO2000015776A1 (en) * 1998-09-10 2000-03-23 Flinders Technologies Pty. Ltd. Product and method for separation of a sample containing multiple sources of genetic material using a solid medium
US5973138A (en) * 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
FI990082A0 (fi) * 1999-01-18 1999-01-18 Labsystems Oy Puhdistusprosessi magneettipartikkeleita käyttäen
US7790865B1 (en) 1999-02-02 2010-09-07 Qiagen North American Holdings, Inc Eluting reagents, methods and kits for isolating DNA
EP1173623B1 (de) * 1999-03-11 2008-06-25 Whatman, Inc. Festmedium sowie verfahren zur speicherung und schnellen aufreinigung von nukleinsäuren
JP2002541839A (ja) * 1999-04-21 2002-12-10 アノビス， インコーポレイテッド 植物についての磁気ｄｎａ抽出キット
DE60026641T2 (de) * 1999-07-15 2007-01-25 Qiagen Gmbh Verfahren zur Trennung von teilchenförmigen Substraten aus einer Lösung indem man den Teilchenverlust minimiert
US6936414B2 (en) * 1999-12-22 2005-08-30 Abbott Laboratories Nucleic acid isolation method and kit
GB0001450D0 (en) 2000-01-21 2000-03-08 Genpoint As Cell isolation method
GB0013658D0 (en) * 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
JP2004508057A (ja) * 2000-09-06 2004-03-18 ホッジ　ティモシー　エイ トランスジェニック変異および標的特定変異をスクリ−ニングするためのシステム、方法並びに装置
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US7011943B2 (en) * 2000-09-06 2006-03-14 Transnetyx, Inc. Method for detecting a designated genetic sequence in murine genomic DNA
WO2002055727A2 (en) * 2001-01-09 2002-07-18 Whitehead Biomedical Inst Methods and reagents for the isolation of nucleic acids
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
JPWO2003006650A1 (ja) * 2001-07-09 2004-11-04 旭化成株式会社 不織布を用いた核酸精製法及び検出法
US7893228B2 (en) 2001-10-12 2011-02-22 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
US20050032105A1 (en) 2001-10-12 2005-02-10 Bair Robert Jackson Compositions and methods for using a solid support to purify DNA
US7148343B2 (en) 2001-10-12 2006-12-12 Gentra Systems, Inc. Compositions and methods for using a solid support to purify RNA
KR100445560B1 (ko) * 2001-10-31 2004-08-21 (주)바이오넥스 핵산 또는 생물학적 물질을 분리하기 위한 키트의 제조방법과, 그 방법에 의해 제조된 키트와, 그 키트를사용하는 장치
JP4106026B2 (ja) * 2001-11-28 2008-06-25 アプレラ コーポレイション 選択的な核酸の単離方法および組成物
DE60316660T3 (de) 2002-01-08 2016-01-28 Roche Diagnostics Gmbh Verwendung eines silikamaterials bei der amplifikation
EP1468116A2 (de) 2002-01-16 2004-10-20 Dynal Biotech ASA Verfahren zur isolierung von nukleinsäuren und proteinen aus einer einzigen probe
US7786295B2 (en) 2002-02-15 2010-08-31 Qiegen North American Holdings, Inc Method to isolate DNA
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
EP1388588B1 (de) * 2002-07-29 2008-09-17 JSR Corporation Methode zur Separierung von Nukleinsäuren und dafür verwendbare Reagenz
ES2320875T5 (es) † 2002-10-04 2012-10-30 Ge Healthcare Bio-Sciences Corp. Procedimientos y materiales para utilizar compuestos químicos como herramienta para el almacenamiento de ácidos nucleicos sobre medios de sistemas de purificación de ácidos nucleicos
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
EP1633869A1 (de) * 2003-06-04 2006-03-15 Qiagen AS Verfahren zur sequenziellen isolierung von dns und rns aus derselben nukleinsäure-enthaltenden probe
JP4871722B2 (ja) † 2003-07-25 2012-02-08 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 固定試料からｒｎａを調製するための方法および組成物
EP1654066B1 (de) 2003-07-31 2014-11-12 Handylab, Inc. Verarbeitung von teilchenhaltigen proben
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
CA2565572C (en) * 2004-05-03 2018-03-06 Handylab, Inc. A microfluidic device and methods for processing polynucleotide-containing samples
EP1756136B1 (de) 2004-05-21 2014-10-29 Mo Bio Laboratories, Inc. Kits und verfahren zur entfernung von verunreinigungen aus nukleinsäuren bei biologischen proben und umweltproben
WO2006004611A2 (en) * 2004-06-25 2006-01-12 Invitrogen Corporation Separation of nucleic acid
CA2575140A1 (en) 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US20060105372A1 (en) 2004-11-05 2006-05-18 Bair Robert J Compositions and methods for purifying nucleic acids from stabilization reagents
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
CA2597919A1 (en) 2005-02-15 2006-08-24 University Of Virginia Patent Foundation Nucleic acid isolation methods and materials and devices thereof
CA2842402C (en) * 2005-03-10 2016-02-23 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US20100063263A1 (en) 2005-06-23 2010-03-11 Siemens Medical Solutions Diagnostics Gmbh Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use
DE102005047736B4 (de) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
ES2692380T3 (es) 2006-03-24 2018-12-03 Handylab, Inc. Método para realizar PCR con un cartucho con varias pistas
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
WO2008002882A2 (en) * 2006-06-26 2008-01-03 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US8163535B2 (en) * 2006-06-26 2012-04-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
US20080003574A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Kits for RNA extraction
DE102006032610C5 (de) * 2006-07-11 2016-09-15 Aj Innuscreen Gmbh Verfahren zur parallelen Isolierung viraler Nukleinsäuren
US10131935B2 (en) 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
NZ576694A (en) 2006-11-06 2012-03-30 Clondiag Gmbh Device and process for assays using binding members
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2132311A1 (de) * 2007-03-21 2009-12-16 Ibis Biosciences, Inc. Reagentien zur nukleinsäurereinigung
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
DK2171098T3 (en) * 2007-06-29 2018-05-22 Becton Dickinson Co PROCEDURES FOR EXTRACTION AND CLEANING COMPONENTS IN BIOLOGICAL SAMPLES
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
WO2009012185A1 (en) 2007-07-13 2009-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
AU2008280121B2 (en) 2007-07-23 2015-02-26 Clondiag Gmbh Assays
US20110300608A1 (en) * 2007-09-21 2011-12-08 Streck, Inc. Nucleic acid isolation in preserved whole blood
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
ES2588908T3 (es) 2007-11-21 2016-11-07 Cosmosid Inc. Sistema de identificación de genoma
US8478544B2 (en) 2007-11-21 2013-07-02 Cosmosid Inc. Direct identification and measurement of relative populations of microorganisms with direct DNA sequencing and probabilistic methods
CN102257158B (zh) 2008-02-15 2014-12-10 生命科技公司 用于dna提取的方法和试剂盒
DE102008010693A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
DE102008010692A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrices, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
CL2009000647A1 (es) * 2008-04-04 2010-06-04 Chugai Pharmaceutical Co Ltd Composicion farmaceutica para tratar o prevenir cancer hepatico que comprende una combinacion de un agente quimioterapeutico y un anticuerpo anti-glipicano 3; agente para atenuar un efecto secundario que comprende dicho anticuerpo; metodo para tratar o prevenir un cancer hepatico de un sujeto.
DE102008029356A1 (de) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
CA2742598A1 (en) * 2008-11-04 2010-05-14 Blood Cell Storage, Inc. Nucleic acid extraction on curved glass surfaces
JP5570422B2 (ja) 2009-01-16 2014-08-13 アークレイ株式会社 核酸試料の製造方法、および、それを用いた核酸増幅物の製造方法
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
TW201113523A (en) * 2009-08-31 2011-04-16 Mbio Diagnostics Inc Integrated sample preparation and analyte detection
US20110223588A1 (en) * 2010-03-09 2011-09-15 Biosample Llc Solid Phase Nucleic Acid Extraction From Small Sample Volumes, and Release of Controlled Quantities
PL2588609T3 (pl) 2010-06-29 2018-06-29 Exscale Biospecimen Solutions Ab Sposób i zestaw do sekwencyjnej izolacji nukleotydów z próbki
US8747084B2 (en) 2010-07-21 2014-06-10 Aperia Technologies, Inc. Peristaltic pump
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
GB201020095D0 (en) 2010-11-26 2011-01-12 Invitrogen Dynal As Nucleic acid preparation method
MX2013008252A (es) * 2011-01-18 2014-04-14 Everist Genomics Inc Firma de pronostico para la recurrencia de cancer colorrectal.
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
EP3709025A1 (de) 2011-04-15 2020-09-16 Becton, Dickinson and Company Mikrofluidischer abtastender echtzeit-thermocycler und verfahren zur optischen detektion mittels synchronisierter thermozyklierung und abtastung
WO2012159063A2 (en) 2011-05-19 2012-11-22 Blood Cell Strorage, Inc. Gravity flow fluidic device for nucleic acid extraction
EP2546199A1 (de) 2011-07-14 2013-01-16 Université Catholique De Louvain Solider Eisenoxidträger zur Isolierung von Mikroorganismen
JP5924888B2 (ja) * 2011-08-26 2016-05-25 関東化學株式会社 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
EP2761305B1 (de) 2011-09-30 2017-08-16 Becton, Dickinson and Company Vereinheitlichter reagenzienstreifen
WO2013067509A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
US10053722B2 (en) * 2011-12-21 2018-08-21 Geneohm Sciences Canada Inc. Enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample
AU2013214849B2 (en) 2012-02-03 2016-09-01 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
WO2013119894A1 (en) 2012-02-09 2013-08-15 Life Technologies Corporation Hydrophobic diacrylamide compound
WO2013119956A1 (en) 2012-02-09 2013-08-15 Life Technologies Corporation Conjugated polymeric particle and method of making same
KR102081560B1 (ko) 2012-02-09 2020-02-25 라이프 테크놀로지스 코포레이션 친수성 중합체성 입자 및 그의 제조 방법
EP2814597A4 (de) 2012-02-15 2015-10-28 Bio Rad Laboratories Elektronische steuerung von ph-wert und ionenstärke
WO2013126620A1 (en) 2012-02-23 2013-08-29 Dow Agrosciences Llc Automation of barrier-based plant nucleic acid and protein extraction
PL2828103T3 (pl) 2012-03-20 2017-09-29 Aperia Technologies System pompowania opon
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
WO2014018195A1 (en) 2012-06-21 2014-01-30 Monsanto Technology Llc Lysis buffer and methods for extraction of dna from plant material
US9206417B2 (en) 2012-07-19 2015-12-08 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
CN103571824B (zh) 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
US20150252356A1 (en) 2012-08-28 2015-09-10 Bio Cube System Co., Ltd. Porous solid phase for rapidly isolating biological molecules for nucleic acid amplification reaction from biological sample, and use thereof
GB201216387D0 (en) 2012-09-13 2012-10-31 Ge Healthcare Uk Ltd Solid matrix for one step nucleic acid amplification
US10144254B2 (en) 2013-03-12 2018-12-04 Aperia Technologies, Inc. Tire inflation system
US11453258B2 (en) 2013-03-12 2022-09-27 Aperia Technologies, Inc. System for tire inflation
US9604157B2 (en) 2013-03-12 2017-03-28 Aperia Technologies, Inc. Pump with water management
US10052428B2 (en) 2013-03-15 2018-08-21 Fenwal, Inc. Methods and systems for the filterless reduction of leukocytes in a biological fluid
AU2014332956B2 (en) * 2013-10-07 2018-01-25 Mitsui Chemicals, Inc. PCR primer set for bacterial DNA amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
EP4148141A1 (de) 2014-02-10 2023-03-15 Zymo Research Corporation Kit zur nukleinsäureerfassung
US20150252407A1 (en) * 2014-03-09 2015-09-10 Nvigen, Inc. Nanostructure and methods of nucleic acid isolation
SG11201609008PA (en) 2014-04-29 2016-11-29 Accudx Corp A novel affinity matrix and devices for isolation and purification of rna and dna for point of care molecular devices
US9879223B2 (en) 2014-07-30 2018-01-30 Fenwal, Inc. Methods and systems for the reduction of leukocytes in a biological fluid
JP6437250B2 (ja) 2014-09-03 2018-12-12 株式会社ミズホメディー 前処理方法及びそれに用いられる核酸抽出キット
US10000750B2 (en) 2014-10-09 2018-06-19 Promega Corporation Method of isolating nucleic acid
EP3218480A1 (de) 2014-11-14 2017-09-20 Corning Incorporated Verfahren und kits zur rna-reinigung nach ivt
CN107208136A (zh) * 2014-12-18 2017-09-26 通用电气医疗集团英国有限公司 通过与免疫测定法偶联的核酸扩增的固体支持物上的分析物检测
GB201504459D0 (en) * 2015-03-17 2015-04-29 Moorlodge Biotech Ventures Ltd Isolation of DNA
US9422596B1 (en) 2015-04-27 2016-08-23 Norgen Biotek Corp. Methods and columns for nucleic acid purification
EP3317426B1 (de) 2015-07-02 2020-01-15 Life Technologies Corporation Konjugation von funktionellen hydrophilen carboxylkügelchen
CN108064253B (zh) 2015-07-02 2020-09-15 生命技术公司 由羧基官能丙烯酰胺形成的聚合物基质
WO2017007774A1 (en) 2015-07-06 2017-01-12 Life Technologies Corporation Substrates and methods useful in sequencing
EP3344643A4 (de) 2015-09-04 2019-05-01 Qiagen Sciences LLC Verfahren zur gemeinsamen isolierung von nukleinsäuren und proteinen
WO2017044710A1 (en) * 2015-09-11 2017-03-16 Corning Incorporated Compositions and methods for nucleic acid purification from blood samples
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
US20190135774A1 (en) * 2016-04-21 2019-05-09 Cell Data Sciences, Inc. Biomolecule processing from fixed biological samples
CA3022947A1 (en) 2016-05-04 2017-11-09 Children's Hospital & Research Center At Oakland Rapid extraction of nucleic acids from clinical samples for downstream applications
MX2019002569A (es) 2016-09-06 2019-10-07 Aperia Tech Inc Sistema para inflado de neumáticos.
DE102016121483B4 (de) 2016-11-09 2020-06-18 Axagarius Gmbh & Co. Kg Partikelförmiges Feststoff-Kompositmaterial zur Nukleinsäureaufreinigung, enthaltend magnetische Nanopartikel , Verfahren zu dessen Herstellung und dessen Verwendung
US11129673B2 (en) 2017-05-05 2021-09-28 Uptake Medical Technology Inc. Extra-airway vapor ablation for treating airway constriction in patients with asthma and COPD
CN111356770B (zh) 2017-09-18 2023-12-08 相达生物科技国际有限公司 使用双水相系统来分离、纯化和/或浓缩短链核酸片段的方法
US10406869B2 (en) 2017-11-10 2019-09-10 Aperia Technologies, Inc. Inflation system
WO2020112686A1 (en) 2018-11-27 2020-06-04 Aperia Technologies, Inc. Hub-integrated inflation system
TWI733302B (zh) * 2020-01-09 2021-07-11 創想生物科技有限公司 核酸分離方法及其系統
KR20220075978A (ko) 2020-11-30 2022-06-08 (주)바이오니아 중저 비유전율을 가진 화합물을 포함한 핵산 결합 완충액을 이용한 핵산 분리방법
CN112626174B (zh) * 2020-12-16 2024-05-07 广州源井生物科技有限公司 一种细胞裂解液及其应用

Family Cites Families (229)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US873102A (en) 1907-06-21 1907-12-10 William W Sly Clutch.
SE328534B (de) * 1965-08-27 1970-09-21 Astra Ab
US4055469A (en) 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
JPS598164B2 (ja) 1977-11-30 1984-02-23 三菱重工業株式会社 防塵マスクなどのフイルタ材のなめし処理装置
AU530410B2 (en) * 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
JPS5940227B2 (ja) * 1980-06-24 1984-09-28 富士通株式会社 リアクティブスパッタリング方法
DE3132491A1 (de) * 1980-08-22 1982-07-01 Laboratorium Prof. Dr. Rudolf Berthold, 7547 Wildbad Verfahren zur durchfuehrung analytischer bestimmungen mittels der chemilumineszenzmethode und anwendung des verfahrens fuer immunassays
US4379843A (en) * 1981-01-26 1983-04-12 Peter Cashion Immobilization of polynucleotides and polypeptides with tritylated polysaccharides
US5055556A (en) 1981-10-06 1991-10-08 The Board Of Trustees Of The Leland Stanford Jr. Univ. Fluorescent conjugates for analysis of molecules and cells
IL65131A0 (en) * 1982-02-28 1982-04-30 Yeda Res & Dev Process for the production of agarose-polyaldehyde beads and their biological applications
NO155316C (no) 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
US4695537A (en) * 1982-05-21 1987-09-22 The University Of Tennessee Research Corp. Particles sensitized with detergent-treated antigen for agglutination immunoassay
GR79124B (de) 1982-12-22 1984-10-02 Genentech Inc
US4861705A (en) 1983-01-31 1989-08-29 Yeda Research And Development Company, Ltd. Method for removing components of biological fluids
DE3310337A1 (de) 1983-03-22 1984-09-27 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Verfahren zur durchfuehrung von hybridisierungsreaktionen
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4648975A (en) * 1983-08-17 1987-03-10 Pedro B. Macedo Process of using improved silica-based chromatographic supports containing additives
US4920061A (en) * 1984-03-02 1990-04-24 The University Of Texas System Biological magnetic colloids
US4652517A (en) 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
AU571979B2 (en) 1984-07-06 1988-04-28 Ciba-Geigy Ag Ionically modified polysaccharides
GB8501671D0 (en) * 1985-01-23 1985-02-27 Allen G J Immunoassay
GB8507706D0 (en) 1985-03-25 1985-05-01 Genetics Int Inc Magnetic nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4897444A (en) * 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
US4710472A (en) 1985-09-25 1987-12-01 The United States Of America As Represented By The Secretary Of The Navy Magnetic separation device
US4889916A (en) 1985-11-19 1989-12-26 The Johns Hopkins University Protein label and drug delivery system
US5076950A (en) * 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
US4772550A (en) 1986-02-10 1988-09-20 Miles Inc. Heterogeneous specific binding assay employing an aggregatable binding reagent
US4871433A (en) 1986-04-04 1989-10-03 Materials Research Corporation Method and apparatus for improving the uniformity ion bombardment in a magnetron sputtering system
US6881536B1 (en) * 1986-04-30 2005-04-19 Bioveris Corporation Particle based electrochemiluminescent assays
FR2600655B1 (fr) 1986-06-30 1988-10-21 Centre Nat Rech Scient Fraction proteique extraite des activites facteurs de croissance, obtention et application comme agent de stimulation et de potentialisation des activites facteurs de croissance
US4843012A (en) * 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
DE3639949A1 (de) 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4767670A (en) 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
US4874813A (en) 1987-02-09 1989-10-17 Shannessy Daniel J O Proteins bound to a marker or solid phase support matrix using a hydrazone linkage
NO162946C (no) * 1987-08-21 1990-03-14 Otto Soerensen Anordning for magnetisk separasjon av celler.
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
US5079155A (en) * 1987-03-02 1992-01-07 E. I. Du Pont De Nemours And Company Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
ATE107654T1 (de) 1987-03-02 1994-07-15 Gen Probe Inc Polykationische träger zur reinigung, trennung und hybridisierung von nukleinsäure.
DE3717211A1 (de) 1987-05-22 1988-12-01 Diagen Inst Molekularbio Vorrichtung und verfahren zur trennung und reinigung von molekuelen
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
JPH01125395A (ja) 1987-07-29 1989-05-17 Life Technol Inc 核酸浦獲試薬
FR2619568B1 (fr) 1987-08-21 1989-07-07 Centre Nat Rech Scient Polymeres derives du polystyrene, leurs procedes de preparation et leurs applications pour l'analyse et la purification de molecules d'origine biologique
US4908318A (en) * 1987-09-04 1990-03-13 Integrated Genetics, Inc. Nucleic acid extraction methods
US5585481A (en) 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
GB8800702D0 (en) * 1988-01-13 1988-02-10 Nycomed As Test method & reagent kit therefor
US5183809A (en) * 1990-02-15 1993-02-02 The Trustees Of The University Of Pennsylvania/Childrens Hospital Corporation Cyclodextrin polymers and cyclodextrins immobilized on a solid surface
US4875045A (en) 1988-03-09 1989-10-17 Northern Telecom Limited Variable gain encoder apparatus and method
JP2791367B2 (ja) 1988-04-21 1998-08-27 マイクロプローブ・コーポレーション 核酸抽出方法
US4952519A (en) 1988-05-02 1990-08-28 E. I. Du Pont De Nemours And Company Protein immobilization with poly(ethyleneimine) derivatized with a hydroprobic group
DE3921498A1 (de) * 1988-09-28 1990-03-29 Bayer Ag Polymer-gebundene-farbstoffe, verfahren zu deren herstellung und verwendung
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
CA1335181C (en) 1988-10-11 1995-04-11 R. Alan Hardwick System for selective cell separation from cell concentrate
IE883226L (en) 1988-10-25 1990-04-25 Dna Prep Galway Ltd S 22 Separation of nucleic acids from protein material using¹cation exchangers
US5028696A (en) 1988-10-28 1991-07-02 Torres Anthony R Ion exchange and separation method
US6448091B1 (en) 1988-11-03 2002-09-10 Igen International, Inc. Method and apparatus for improved luminescence assays using particle concentration chemiluminescence detection
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
CA2005927A1 (en) * 1988-12-21 1990-06-21 Chander Bahl Method of preparing nucleotide probes using a bridging complement
WO1990008159A1 (en) 1989-01-23 1990-07-26 Invitron Corporation Method for removing dna from protein preparations
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
JP2910929B2 (ja) * 1989-03-23 1999-06-23 浜松ホトニクス株式会社 磁気粒子よりなる粒子の表面改質方法
US5126028A (en) 1989-04-17 1992-06-30 Materials Research Corporation Sputter coating process control method and apparatus
US4957605A (en) 1989-04-17 1990-09-18 Materials Research Corporation Method and apparatus for sputter coating stepped wafers
US5334499A (en) 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US6010867A (en) * 1989-04-19 2000-01-04 Ibiden Co., Ltd. Reagent for biomaterials assay, preparation method thereof, and assay method
DE3916595A1 (de) 1989-05-22 1990-11-29 Boehringer Mannheim Gmbh Verfahren zur nichtradioaktiven messung der nucleinsaeuresynthese in eukaryontischen zellen
NO168811C (no) 1989-05-31 1992-04-08 Dynal As Separasjonsanordning for separering av magnetiserbare partikler
US5124444A (en) * 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
ATE103507T1 (de) 1989-08-07 1994-04-15 Baker J T Inc Feste traeger aus quaternisierter pei-kieselsaeure fuer die chromatographie.
US5032281A (en) * 1989-08-09 1991-07-16 Daicel Chemical Industries, Ltd. Separating membrane and separation method
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5084169A (en) * 1989-09-19 1992-01-28 The University Of Colorado Foundation, Inc. Stationary magnetically stabilized fluidized bed for protein separation and purification
US5433847A (en) * 1989-11-01 1995-07-18 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Radial flow chromatography
EP0500727B1 (de) 1989-11-13 1998-01-21 Children's Medical Center Corporation Ein nichtinvasives verfahren zur trennung und zum nachweis von fetaler dna
US4997932A (en) * 1989-11-13 1991-03-05 Boehringer Mannheim Corporation Method and kit for purifying nucleic acids
US5641628A (en) * 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
US5000635A (en) * 1989-11-17 1991-03-19 The Boeing Company Spring-loaded outboard side lock
WO1991008308A1 (en) 1989-11-30 1991-06-13 Pharmacia Genetic Engineering, Inc. A new method for detecting a specific nucleic acid sequence in a sample of cells
US5523231A (en) * 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
CA2082936C (en) 1990-05-15 2003-09-23 Peter O. G. Arrhenius Fluorescent porphyrin, and fluorescent phthalocyanine-polyethylene glycol, polyol, and saccharide derivatives as fluorescent probes
US5234924A (en) 1990-09-26 1993-08-10 Adir Et Compagnie Benzothiazine and benzothiazole compounds useful as analgesics
DE4034036C2 (de) 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5234824A (en) 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5654179A (en) 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
DE4038293A1 (de) 1990-11-28 1992-06-04 Inst Molekularbiologie Ak Nucleinsaeure-addukte, verfahren zu ihrer herstellung und ihrer verwendung zur in situ polymerase-kettenreaktion
US5204246A (en) * 1990-12-26 1993-04-20 Pioneer Hi-Bred International, Inc. Dna isolation method
US5296347A (en) * 1991-02-08 1994-03-22 Ciba Corning Diagnostics Corp. Bridge immunoassay
US5491223A (en) * 1991-02-28 1996-02-13 Daicel Chemical Industries, Ltd. Polysaccharide derivative and separating agent
GB9107124D0 (en) 1991-04-05 1991-05-22 Dynal As Chemical process
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US6251581B1 (en) * 1991-05-22 2001-06-26 Dade Behring Marburg Gmbh Assay method utilizing induced luminescence
US5155018A (en) 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
FR2679255B1 (fr) * 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
WO1993003167A1 (en) 1991-08-06 1993-02-18 The World Health Organisation Method of isolation of dna
US5329000A (en) * 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
EP0555798B1 (de) 1992-02-13 1999-05-06 Becton, Dickinson and Company Celithydrat und Reinigung von DNS
DE4208645A1 (de) * 1992-03-18 1993-09-23 Bayer Ag Optischer festphasenbiosensor auf basis fluoreszenzfarbstoffmarkierter polyionischer schichten
DE4237381C1 (de) 1992-11-05 1994-04-28 Diagen Inst Molekularbio Trägermaterial zur simultanen Bindung genotypischer und phänotypischer Substanzen
GB9212164D0 (en) 1992-06-09 1992-07-22 Medical Res Council Preparation of nucleic acids
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
GB9220773D0 (en) 1992-10-02 1992-11-18 Mini Agriculture & Fisheries Detection of antigens and nucleic acids
GB2272391A (en) 1992-11-05 1994-05-18 Gordon Henry Turner Drilling jig
GB9223334D0 (en) 1992-11-06 1992-12-23 Hybaid Ltd Magnetic solid phase supports
US5672659A (en) 1993-01-06 1997-09-30 Kinerton Limited Ionic molecular conjugates of biodegradable polyesters and bioactive polypeptides
JP3607289B2 (ja) * 1993-03-29 2005-01-05 ミネソタ マイニング アンド マニュファクチャリング カンパニー 多孔質支持体（ｐ．ｅ．アズラクトン）の内部にリガンドをカップリングする方法及びその使用法
US5438129A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
US5503816A (en) * 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
GB2282138B (en) 1993-09-28 1997-09-03 Tosoh Corp Method of extracting nucleic acids and method of detecting specified nucleic acid sequences
US5919455A (en) 1993-10-27 1999-07-06 Enzon, Inc. Non-antigenic branched polymer conjugates
US20030044777A1 (en) * 1993-10-28 2003-03-06 Kenneth L. Beattie Flowthrough devices for multiple discrete binding reactions
GB9323305D0 (en) 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
DE4432654C2 (de) 1994-09-14 1998-03-26 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus natürlichen Quellen
CN1150431A (zh) 1994-04-08 1997-05-21 海布里顿公司 用阴离子交换层析法纯化寡脱氧核苷酸硫代磷酸酯
WO1996000228A1 (en) 1994-06-23 1996-01-04 Dade International Inc. Method for the rapid isolation of nucleic acid
BR9508628A (pt) 1994-08-25 1998-06-02 Iseki Kaihatsu Koki Máquina de escavação de orifício vertical
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5631146A (en) * 1995-01-19 1997-05-20 The General Hospital Corporation DNA aptamers and catalysts that bind adenosine or adenosine-5'-phosphates and methods for isolation thereof
US5762903A (en) 1995-03-10 1998-06-09 Korea Atomic Energy Research Institute Radioactive chitosan complex for radiation therapy
CA2219675C (en) * 1995-04-28 2009-08-18 Daiichi Pharmaceutical Co., Ltd. Pentacyclic compound
US5702147A (en) * 1995-05-09 1997-12-30 Bestop, Inc. Tailgate sealing arrangement
AR003122A1 (es) 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
KR19990022612A (ko) 1995-06-08 1999-03-25 퍼니스 챨스 엠 디엔에이 추출방법 및 추출장치
DE19520398B4 (de) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
DE69612390T2 (de) * 1995-08-28 2001-10-31 Advanced Nano Technologies Pty. Ltd., Nedlands Verfahren zur herstellung ultrafeiner teilchen
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
EP0866802A1 (de) 1995-11-21 1998-09-30 Novartis AG Multivalente polymere, deren herstellung und verwendung zur herstellung von biologisch aktiven verbindungen
US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
SE9600590D0 (sv) * 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
AU1811197A (en) 1996-02-25 1997-09-10 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
NO961221D0 (no) 1996-03-26 1996-03-26 Oeystein Fodstad Fremgangsmåte til å identifisere nye gener assosiert med setepreferert cancer mestastasedannelse
SE9601318D0 (sv) * 1996-04-04 1996-04-04 Pharmacia Biosensor Ab Method for nucleic acid analysis
US5780319A (en) * 1996-04-19 1998-07-14 Pasteur Sanofi Diagnostics Immunoassays to detect antiphospholipid antibodies
US5916746A (en) * 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay
US5962412A (en) 1996-06-10 1999-10-05 Arqule, Inc. Method of making polymers having specific properties
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
JP3793287B2 (ja) 1996-08-22 2006-07-05 ペンタックス株式会社 プラスミドｄｎａの単離方法
CA2214495C (en) 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
US5795748A (en) 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
EP0938549A1 (de) * 1996-10-10 1999-09-01 Biotechnology Research And Development Corporation Polymer-protein verbundwerkstoff und verfahren zur herstellung und verwendung
ATE259056T1 (de) 1996-11-06 2004-02-15 Sequenom Inc Verfahren zur massenspektrometrie
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
EP0853123A1 (de) 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US5770712A (en) * 1997-03-14 1998-06-23 Virginia Tech Intellectual Properties, Inc. Crosslinked hydrogel beads from chitosan
GB9709728D0 (en) 1997-05-13 1997-07-02 Dynal As Single step method
US20040152084A1 (en) 2003-01-31 2004-08-05 Slattum Paul M. Compounds and processes for single-pot attachment of a label to nucleic acid
US5958788A (en) 1997-05-28 1999-09-28 Nalco Chemical Company Luminol tagged polymers for treatment of industrial systems
US6218531B1 (en) * 1997-06-25 2001-04-17 Promega Corporation Method of isolating RNA
EP0897978A3 (de) 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirkoniumoxyd und verwandten Verbindungen zur Reinigung von Nukleinsäuren
DE19739218A1 (de) 1997-09-08 1999-03-11 Boehringer Mannheim Gmbh Aufreinigung von Substanzen aus einer biologischen Probe
JP4304348B2 (ja) 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
AU9527098A (en) 1997-10-23 1999-05-17 Nicholas A. D. Burke Labelling of polymers and sequencing of nucleic acids
DE19746874A1 (de) 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
DE19747572C1 (de) 1997-10-28 1999-04-08 Inst Chemo Biosensorik Vorrichtung und Verfahren zur Durchführung von Fluoreszenzimmuntests
US6120985A (en) 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
DE69839133T2 (de) * 1997-12-06 2009-02-05 Invitrogen Corp., Carlsbad Isolierung von Nukleinsäuren
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
BR9907852A (pt) * 1998-02-12 2000-10-24 Immunivest Corp Processos para detectar e enumerar células raras e cancerosas em uma população celular mista, para diagnosticar câncer de estágio precoce em um paciente de teste, para determinar a probabilidade de recorrência de câncer em um paciente humano anteriormente tratado de câncer, para distinguir um carcinoma confinado ao órgão de um carcinoma com propriedades metastáticas, para acompanhar a situação de remissão em um paciente humano com câncer que passa pelo tratamento de terapia contra o câncer e para aumentar quantidades de células epiteliais circulantes em uma amostra de sangue, partìcula magnética revestida, composição, conjuntos de teste para avaliar uma amostra de paciente quanto a presença de células raras circulantes, quanto a presença de células de tumor circulantes, quanto a presença de células de câncer de mama circulantes, quanto a presença de células de câncer de próstata circulantes, quanto a presença de células de câncer de cólon circulantes, quanto a presença de células de câncer de bexiga circulantes e para monitorar um paciente quanto a recorrência de câncer, e, fração de sangue periférico enriquecido quanto a células neoplásticas circulantes
FR2777355B1 (fr) 1998-04-10 2000-05-12 Bio Merieux Procede de fixation d'une molecule biologique sur la surface d'un support constitue de silice ou d'oxyde metallique
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US6287772B1 (en) * 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6287765B1 (en) 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US6245507B1 (en) * 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
AU2025400A (en) 1998-11-17 2000-06-05 Proteo Tools Separation, screening, and identification of biological targets
DE69929826D1 (de) 1998-11-23 2006-04-20 Us Gov Sec Army Reinigungsmethode und vorrichtung
FI990082A0 (fi) 1999-01-18 1999-01-18 Labsystems Oy Puhdistusprosessi magneettipartikkeleita käyttäen
EP1147226B1 (de) * 1999-01-27 2013-01-23 Folim G. Halaka Materialien und verfahren zur reinigung von polyelektrolyten
CA2360042A1 (en) 1999-01-29 2000-08-03 Amersham Pharmacia Biotech K.K. Temperature-responsive polymer compound and process for producing the same
DE19907023A1 (de) 1999-02-19 2000-08-24 Bayer Ag Verfahren zur Isolierung von Nucleinsäuren
GB9907245D0 (en) 1999-03-29 1999-05-26 Goldsborough Andrew Cleavage of nucleic acids from solid supports
WO2000061806A2 (en) 1999-04-09 2000-10-19 Arcturus Engineering, Inc. GENERIC cDNA OR PROTEIN ARRAY FOR CUSTOMIZED ASSAYS
CA2270106C (en) 1999-04-23 2006-03-14 Yousef Haj-Ahmad Nucleic acid purification and process
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
ATE530648T1 (de) 1999-05-14 2011-11-15 Promega Corp Zell konzentration und lysat klärung mittels paramagnetischen partikeln
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6645733B1 (en) 1999-06-25 2003-11-11 Ingeneus Corporation Fluorescent intensity method for assaying binding between proteins or peptides
DE60026641T2 (de) * 1999-07-15 2007-01-25 Qiagen Gmbh Verfahren zur Trennung von teilchenförmigen Substraten aus einer Lösung indem man den Teilchenverlust minimiert
WO2001038585A2 (en) 1999-11-24 2001-05-31 The Regents Of The University Of California Polymer arrays and methods of using labeled probe molecules to identify and quantify target molecule expression
US6582907B1 (en) * 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
US6638906B1 (en) * 1999-12-13 2003-10-28 Nobex Corporation Amphiphilic polymers and polypeptide conjugates comprising same
EP1134294A3 (de) 2000-03-16 2004-06-30 Kabushiki Kaisha Toshiba Verfahren zur Synthese eines immobilisierten Nukleinsäurestranges
US7078536B2 (en) 2001-03-14 2006-07-18 Genesoft Pharmaceuticals, Inc. Charged compounds comprising a nucleic acid binding moiety and uses therefor
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
GB0013658D0 (en) 2000-06-05 2000-07-26 Dynal Asa Nucleic acid isolation
WO2002010373A2 (en) 2000-07-31 2002-02-07 Applera Corporation Apparatus and method for specific release of captured extension products
WO2002016580A2 (en) 2000-08-24 2002-02-28 Bio-Rad Laboratories, Inc. Flow-through system for lysate clarification and nucleic acid binding in a single step
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
GB0026560D0 (en) * 2000-10-30 2000-12-13 Novartis Ag Organic compounds
DE60015360T9 (de) 2000-11-20 2005-12-15 Sony International (Europe) Gmbh Verfahren zur Immobilisierung von Nukleinsäuren auf festem Träger
US6743586B2 (en) * 2001-01-29 2004-06-01 The United States Of America As Represented By The Secretary Of The Commerce Rapid fluorescence detection of binding to nucleic acid drug targets labelled with highly fluorescent nucleotide base analogs
JP3975319B2 (ja) 2001-03-02 2007-09-12 富士ゼロックス株式会社 現像装置及びこれを用いた画像形成装置
EP1363954B1 (de) * 2001-03-02 2012-10-17 University of Western Ontario Polymerische vorläufer radiomarkierter verbindungen, und verfahren zu deren herstellung und deren verwendung
AU2002258997A1 (en) 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7338805B2 (en) * 2001-05-04 2008-03-04 Bio Merieux Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
US7183116B2 (en) * 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
WO2003017938A2 (en) * 2001-08-22 2003-03-06 Watson Pharmaceuticals, Inc. Conjugates targeted to target receptors
US20030099954A1 (en) * 2001-11-26 2003-05-29 Stefan Miltenyi Apparatus and method for modification of magnetically immobilized biomolecules
US20030229013A1 (en) 2001-12-07 2003-12-11 Shih-Kwang Wu Solid phase method for synthesis peptide-spacer-lipid conjugates, conjugates synthesized thereby and targeted liposomes containing the same
JP3871677B2 (ja) * 2001-12-27 2007-01-24 松下電器産業株式会社 免疫反応測定方法及びそれに用いる免疫反応測定用試薬キット
US20030147958A1 (en) 2002-01-29 2003-08-07 Cheol-Hee Ahn Biodegradable multi-block copolymers of poly(amino acid)s and poly(ethylene glycol) for the delivery of bioactive agents
AU2003222117A1 (en) * 2002-03-29 2003-10-13 Genentech, Inc. Methods and compositions for detection and quantitation of nucleic acid analytes
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
WO2004001379A2 (en) 2002-06-20 2003-12-31 The Regents Of The University Of California Methods and compositions for detection and analysis of polynucleotides using light harvesting multichromophores
ATE400650T1 (de) 2002-10-18 2008-07-15 Sloning Biotechnology Gmbh Verfahren zur herstellung von nukleinsäuremolekülen
FR2847581B1 (fr) * 2002-11-21 2007-03-23 Commissariat Energie Atomique Procede de fixation d'une proteine sur un polymere a base de pyrrole et son utilisation pour la fabrication d'un capteur
GB0229287D0 (en) 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
US20050123932A1 (en) * 2003-12-09 2005-06-09 Mekbib Astatke Nucleic acid-chelating agent conjugates
BE1016163A6 (fr) 2004-08-18 2006-04-04 Mc Anton Sa Procede d'oxygenotherapie percutanee hyperbare par projection de fluorocarbones.

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DE69533317T2 (de) 2005-12-01
US20110251382A1 (en) 2011-10-13
US7989614B2 (en) 2011-08-02
US20140291576A1 (en) 2014-10-02
US7173124B2 (en) 2007-02-06
US20060058519A1 (en) 2006-03-16
ATE272110T1 (de) 2004-08-15
WO1996018731A3 (en) 1996-09-12
AU706211B2 (en) 1999-06-10
US8691969B2 (en) 2014-04-08
AU4182996A (en) 1996-07-03
EP0796327A2 (de) 1997-09-24
US20090149646A1 (en) 2009-06-11
JP3787354B2 (ja) 2006-06-21
US20070190559A1 (en) 2007-08-16
US20090068724A1 (en) 2009-03-12
US20040215011A1 (en) 2004-10-28
US20080300396A1 (en) 2008-12-04
CA2207608C (en) 2009-04-07
EP0796327B1 (de) 2004-07-28
CA2207608A1 (en) 1996-06-20
GB9425138D0 (en) 1995-02-08

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