DE69030449D1 - Verfahren und kit zur reinigung von nukleinsäure - Google Patents

Verfahren und kit zur reinigung von nukleinsäure

Info

Publication number
DE69030449D1
DE69030449D1 DE69030449T DE69030449T DE69030449D1 DE 69030449 D1 DE69030449 D1 DE 69030449D1 DE 69030449 T DE69030449 T DE 69030449T DE 69030449 T DE69030449 T DE 69030449T DE 69030449 D1 DE69030449 D1 DE 69030449D1
Authority
DE
Germany
Prior art keywords
kit
nucleic acid
purifying nucleic
surfactant
amphotenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69030449T
Other languages
English (en)
Other versions
DE69030449T2 (de
Inventor
Melissa Reardon
Lisa Klein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Operations Inc
Original Assignee
Boehringer Mannheim Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Corp filed Critical Boehringer Mannheim Corp
Application granted granted Critical
Publication of DE69030449D1 publication Critical patent/DE69030449D1/de
Publication of DE69030449T2 publication Critical patent/DE69030449T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
DE69030449T 1989-11-13 1990-07-31 Verfahren und kit zur reinigung von nukleinsäure Expired - Lifetime DE69030449T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/434,324 US4997932A (en) 1989-11-13 1989-11-13 Method and kit for purifying nucleic acids
PCT/US1990/004276 WO1991007422A1 (en) 1989-11-13 1990-07-31 Method and kit for purifying nucleic acids

Publications (2)

Publication Number Publication Date
DE69030449D1 true DE69030449D1 (de) 1997-05-15
DE69030449T2 DE69030449T2 (de) 1997-10-16

Family

ID=23723768

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69033304T Expired - Lifetime DE69033304T2 (de) 1989-11-13 1990-07-31 Verfahren und Montur für die Reinigung von Nukleinsäuren
DE69030449T Expired - Lifetime DE69030449T2 (de) 1989-11-13 1990-07-31 Verfahren und kit zur reinigung von nukleinsäure

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69033304T Expired - Lifetime DE69033304T2 (de) 1989-11-13 1990-07-31 Verfahren und Montur für die Reinigung von Nukleinsäuren

Country Status (6)

Country Link
US (1) US4997932A (de)
EP (3) EP0508985B1 (de)
AT (1) ATE151433T1 (de)
DE (2) DE69033304T2 (de)
ES (1) ES2100887T3 (de)
WO (1) WO1991007422A1 (de)

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US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
SE503729C2 (sv) * 1991-03-11 1996-08-12 Mats Malmquist Extraktionssystem
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren
IT1252174B (it) * 1991-12-09 1995-06-05 Crinos Industria Farmaco Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
WO1994000474A1 (en) * 1992-06-25 1994-01-06 Life Technologies, Inc. Method for the rapid and ultra-sensitive detection and quantification of nucleic acid
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
IL110463A0 (en) * 1993-08-13 1994-10-21 Du Pont In situ extraction of microbial DNA
WO1995021177A1 (de) * 1994-02-07 1995-08-10 Qiagen Gmbh Verfahren zur herstellung endotoxinfreier oder an endotoxin abgereicherter nucleinsäuren und/oder oligonucleotide für die gentherapie
US5990301A (en) * 1994-02-07 1999-11-23 Qiagen Gmbh Process for the separation and purification of nucleic acids from biological sources
US5545539A (en) * 1994-10-18 1996-08-13 Genzyme Corporation Method for nucleotide sequence amplification
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
AU706857B2 (en) * 1995-12-21 1999-06-24 Genzyme Corporation Method for lysing cells
JP4693944B2 (ja) * 1996-03-20 2011-06-01 ベーイーオー・メリュー 核酸単離法
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
ATE248631T1 (de) * 1997-06-27 2003-09-15 Life Technologies Inc Einstufige vorrichtung und verfahren zur konzentration und säuberung biologischer moleküle
US5958677A (en) * 1997-07-28 1999-09-28 The New York Blood Center, Inc. Method for purifying viral nucleic acids
EP1027145A4 (de) * 1997-09-17 2004-08-25 Gentra Systems Inc Vorrichtung und verfahren zur isolierung von nukleinsäure
AU1275899A (en) 1997-10-24 1999-05-17 Megabios Corporation Methods for preparing polynucleotide transfection complexes
US6241980B1 (en) * 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
ZA99493B (en) 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
US6441152B1 (en) * 1998-12-08 2002-08-27 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE19903507A1 (de) 1999-01-29 2000-08-10 Roche Diagnostics Gmbh Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung
AU4367500A (en) * 1999-04-21 2000-11-02 Annovis, Inc. Magnetic dna extraction kit for plants
US20020012982A1 (en) * 2000-07-13 2002-01-31 Invitrogen Corporation Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
EP1305328A2 (de) * 2000-07-21 2003-05-02 Mark B. Lyles Materialien und verfahren zum anlagern von nukleinsäure auf oberflächen
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6833238B2 (en) * 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
ATE454469T1 (de) 2002-01-28 2010-01-15 Ambion Inc Für den nachweis von nukleinsäuren kompetente biologische rohderivate
DE10231659B4 (de) * 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
US20040016702A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Device and method for purification of nucleic acids
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
US20050106602A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Simplified methods for isolating nucleic acids from cellular materials
US20050106577A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Cleavable solid phases for isolating nucleic acids
US20050106576A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Methods of using cleavable solid phases for isolating nucleic acids
US20050136477A1 (en) * 2003-11-17 2005-06-23 Hashem Akhavan-Tafti Methods for isolating nucleic acids from biological and cellular materials
WO2005061532A1 (es) * 2003-12-23 2005-07-07 Berthet Francois Xavier Composiciones y procedimientos para detectar infección patógena
KR20060129340A (ko) * 2003-12-30 2006-12-15 시그마-알드리치컴퍼니 효소 소화에 의한 핵산의 신속한 제조
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
DE102005057334A1 (de) * 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
CA2655458A1 (en) * 2006-06-19 2007-12-27 Becton, Dickinson And Company Methods and compositions for obtaining amplifiable nucleic acids from tissues, cells, or viruses exposed to transport media
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
EP2247753B1 (de) * 2008-02-01 2014-06-25 Siemens Healthcare Diagnostics Inc. Urintransportmittel
EP2367954B1 (de) 2008-12-19 2015-02-18 Life Technologies Corporation Proteinase k inhibitore, verfahren und zusammensetzungen davon
CN102575220B (zh) 2009-09-03 2015-09-16 贝克顿·迪金森公司 用于直接化学裂解的方法和组合物
US9212976B2 (en) * 2013-03-15 2015-12-15 Qiagen Gaithersburg, Inc. Vision-guided aspiration apparatus and methods
EP2970962A4 (de) * 2013-03-15 2017-03-08 Pathogenetix, Inc. Verfahren und vorrichtungen zur herstellung biologischer proben

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FR2471785A1 (fr) * 1979-12-21 1981-06-26 Fabre Sa Pierre Preparations immunostimulantes a base d'arn ribosomaux et procede de preparation des arn
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
DE3211309A1 (de) * 1982-03-26 1983-09-29 Metin Dipl.-Ing. 6100 Darmstadt Colpan Chromatographisches verfahren zur isolierung von makromolekuelen
US4482482A (en) * 1983-01-17 1984-11-13 Pharmacia, Inc. Cesium trifluoroacetate solutions for the isolation of nucleic acids
US4514497B1 (en) * 1983-12-30 1998-02-24 Novagene Inc Modified live pseudorabies viruses
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DE3788082T2 (de) * 1986-04-11 1994-06-09 Applied Biosystems Automatisches Gerät zum Extrahieren von Nukleinsäuren.
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US4833239A (en) * 1987-01-29 1989-05-23 Genetics Institute, Inc. Method for the isolation and purification of DNA molecules

Also Published As

Publication number Publication date
EP0747387A3 (de) 1996-12-18
WO1991007422A1 (en) 1991-05-30
DE69030449T2 (de) 1997-10-16
ATE151433T1 (de) 1997-04-15
EP0747387A2 (de) 1996-12-11
EP0747388B1 (de) 1999-09-22
DE69033304D1 (de) 1999-10-28
EP0747388A1 (de) 1996-12-11
DE69033304T2 (de) 2000-02-03
EP0508985A4 (en) 1992-12-02
US4997932A (en) 1991-03-05
ES2100887T3 (es) 1997-07-01
EP0508985A1 (de) 1992-10-21
EP0508985B1 (de) 1997-04-09

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ROCHE DIAGNOSTICS CORP., INDIANAPOLIS, IND., US

8327 Change in the person/name/address of the patent owner

Owner name: ROCHE DIAGNOSTICS OPERATIONS, INC., INDIANAPOLIS,

8328 Change in the person/name/address of the agent

Representative=s name: DURM & PARTNER, 76137 KARLSRUHE