DE69838839D1 - Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen - Google Patents

Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen

Info

Publication number
DE69838839D1
DE69838839D1 DE69838839T DE69838839T DE69838839D1 DE 69838839 D1 DE69838839 D1 DE 69838839D1 DE 69838839 T DE69838839 T DE 69838839T DE 69838839 T DE69838839 T DE 69838839T DE 69838839 D1 DE69838839 D1 DE 69838839D1
Authority
DE
Germany
Prior art keywords
nucleic acid
amplification reaction
amplification reactions
removing nucleic
acid pollution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69838839T
Other languages
English (en)
Other versions
DE69838839T2 (de
Inventor
Inge Waller Nilsen
Erling Sandsdalen
Even Stenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Norwegian Institute of Fisheries and Aquaculture Ltd
Original Assignee
Norwegian Institute of Fisheries and Aquaculture Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Norwegian Institute of Fisheries and Aquaculture Ltd filed Critical Norwegian Institute of Fisheries and Aquaculture Ltd
Application granted granted Critical
Publication of DE69838839D1 publication Critical patent/DE69838839D1/de
Publication of DE69838839T2 publication Critical patent/DE69838839T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
DE69838839T 1997-08-06 1998-08-06 Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen Expired - Lifetime DE69838839T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9716664.9A GB9716664D0 (en) 1997-08-06 1997-08-06 A method of removing nucleic acid contamination reactions
GB9716664 1997-08-06
PCT/GB1998/002369 WO1999007887A2 (en) 1997-08-06 1998-08-06 A method of removing nucleic acid contamination in amplification reactions

Publications (2)

Publication Number Publication Date
DE69838839D1 true DE69838839D1 (de) 2008-01-24
DE69838839T2 DE69838839T2 (de) 2008-12-04

Family

ID=10817101

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69838839T Expired - Lifetime DE69838839T2 (de) 1997-08-06 1998-08-06 Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen

Country Status (8)

Country Link
US (1) US6541204B2 (de)
EP (1) EP1003913B1 (de)
JP (1) JP3801865B2 (de)
AT (1) ATE380881T1 (de)
AU (1) AU749882B2 (de)
DE (1) DE69838839T2 (de)
GB (1) GB9716664D0 (de)
WO (1) WO1999007887A2 (de)

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US7569342B2 (en) * 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US20080064108A1 (en) * 1997-12-10 2008-03-13 Tony Baker Urine Preservation System
US20020012916A1 (en) * 1998-10-14 2002-01-31 Gundling Gerard J A method of reducing contamination in an essay vessel
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US8361712B2 (en) * 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
AUPS064202A0 (en) * 2002-02-20 2002-03-14 Biomolecular Research Solutions Pty Ltd Improved method for the analysis of nucleic acid samples
AU2003205424B2 (en) * 2002-02-20 2008-11-20 Biomolecular Research Solutions Pty Ltd Removal of nucleic acid contaminants
EP1431387B1 (de) * 2002-12-20 2008-02-27 Roche Diagnostics GmbH Hitzelabile Desoxyribonuklease I-Varianten
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
WO2006009870A2 (en) * 2004-06-17 2006-01-26 Epigenomics Ag Compositions and methods for preventing carry-over contamination in nucleic acid amplification reactions
WO2006034009A2 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
JP4857260B2 (ja) 2005-03-08 2012-01-18 タカラバイオ株式会社 低温性微生物由来エンドヌクレアーゼ
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US9134237B2 (en) * 2005-09-20 2015-09-15 Janssen Diagnotics, LLC High sensitivity multiparameter method for rare event analysis in a biological sample
CA2623405C (en) * 2005-09-20 2014-11-25 Immunivest Corporation Methods and composition to generate unique sequence dna probes labeling of dna probes and the use of these probes
KR100804670B1 (ko) 2006-08-18 2008-02-20 명지대학교 산학협력단 정밀 중합효소 연쇄반응 방법
FR2914317B1 (fr) * 2007-03-30 2012-12-28 Biomerieux Sa Methode de decontamination d'une solution des acides nucleiques indesirables
EP2071034A1 (de) 2007-12-12 2009-06-17 bioMérieux Verfahren zur Behandlung einer Lösung zum Vernichten von Ribonukleinsäure nach der Amplifikation
US20090258365A1 (en) * 2008-03-25 2009-10-15 Terstappen Leon W M M METHOD FOR DETECTING IGF1R/Chr 15 in CIRCULATING TUMOR CELLS USING FISH
WO2009143500A2 (en) * 2008-05-23 2009-11-26 Life Technologies Corporation Methods for removing nucleic acid contamination from reagents
WO2010012464A1 (en) * 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Improved lysis and reverse transcription for mrna quantification
JP5517284B2 (ja) * 2008-09-03 2014-06-11 西川ゴム工業株式会社 耐熱性２本鎖特異的核酸分解酵素
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
MX340406B (es) 2009-01-15 2016-07-07 Nat Univ Corp Univ Of Toyama Preparacion enzimatica que contiene polimerasa de adn termoestable, metodo para su produccion, y metodo para detectar un organismo objeto.
EP2425240A4 (de) 2009-04-30 2012-12-12 Good Start Genetics Inc Verfahren und zusammensetzung zur bewertung genetischer marker
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
ES2576927T3 (es) 2010-10-22 2016-07-12 T2 Biosystems, Inc. Sistemas de RMN y métodos para la detección rápida de analitos
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN104245928B (zh) 2012-02-17 2017-08-18 生物科技药物学会 核酸内切酶
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
WO2016040446A1 (en) * 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
CN105737395A (zh) * 2014-10-13 2016-07-06 崔荀 一种太阳能热水器加辅助电热水箱组合系统
US10066259B2 (en) 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
US11266730B2 (en) 2015-09-29 2022-03-08 The General Hospital Corporation Methods of treating and diagnosing disease using biomarkers for BCG therapy
KR20200118422A (ko) * 2017-12-31 2020-10-15 에버그린 바이오사이언시즈 동결보존용 조성물 및 이의 사용 방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1382386A3 (de) * 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Neue Ordnungen für Oligonukleotide und ihr Nutzen zum Sortieren, Isolieren, Sequenzierung und Manipulieren von Nukleinsäuren
DE4314271A1 (de) * 1993-04-30 1994-11-03 Maiwald Matthias Dr Med Verfahren zur Elimination bakterieller DNA aus hitzestabiler DNA-Polymerase mittels eines DNA verdauenden Enzyms
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
DE19513728A1 (de) * 1995-04-11 1996-10-31 Boehringer Mannheim Gmbh Verfahren zur Reduzierung der Bildung von Artefakten

Also Published As

Publication number Publication date
JP2001512696A (ja) 2001-08-28
WO1999007887A3 (en) 1999-04-29
AU8640298A (en) 1999-03-01
EP1003913A2 (de) 2000-05-31
GB9716664D0 (en) 1997-10-15
DE69838839T2 (de) 2008-12-04
WO1999007887A2 (en) 1999-02-18
EP1003913B1 (de) 2007-12-12
US6541204B2 (en) 2003-04-01
AU749882B2 (en) 2002-07-04
ATE380881T1 (de) 2007-12-15
US20020042052A1 (en) 2002-04-11
JP3801865B2 (ja) 2006-07-26

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