DE3675684D1 - Diagnostisches reagenz, satz und verfahren unter verwendung von polynukleotid-versetzung, trennung, enzymatischer spaltung und nachweis von adenosinphosphat. - Google Patents

Diagnostisches reagenz, satz und verfahren unter verwendung von polynukleotid-versetzung, trennung, enzymatischer spaltung und nachweis von adenosinphosphat.

Info

Publication number
DE3675684D1
DE3675684D1 DE8686104981T DE3675684T DE3675684D1 DE 3675684 D1 DE3675684 D1 DE 3675684D1 DE 8686104981 T DE8686104981 T DE 8686104981T DE 3675684 T DE3675684 T DE 3675684T DE 3675684 D1 DE3675684 D1 DE 3675684D1
Authority
DE
Germany
Prior art keywords
polynucleotide
disposal
separation
detection
enzymatic cleavage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8686104981T
Other languages
English (en)
Inventor
Vary Calvin Pardee Hull
Steven Elliot Diamond
Neil M Wolfman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Honeywell International Inc
Original Assignee
AlliedSignal Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AlliedSignal Inc filed Critical AlliedSignal Inc
Application granted granted Critical
Publication of DE3675684D1 publication Critical patent/DE3675684D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE8686104981T 1985-05-02 1986-04-11 Diagnostisches reagenz, satz und verfahren unter verwendung von polynukleotid-versetzung, trennung, enzymatischer spaltung und nachweis von adenosinphosphat. Expired - Lifetime DE3675684D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/729,503 US4767699A (en) 1985-05-02 1985-05-02 Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine phosphate detection

Publications (1)

Publication Number Publication Date
DE3675684D1 true DE3675684D1 (de) 1991-01-03

Family

ID=24931345

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686104981T Expired - Lifetime DE3675684D1 (de) 1985-05-02 1986-04-11 Diagnostisches reagenz, satz und verfahren unter verwendung von polynukleotid-versetzung, trennung, enzymatischer spaltung und nachweis von adenosinphosphat.

Country Status (5)

Country Link
US (1) US4767699A (de)
EP (1) EP0200057B1 (de)
JP (1) JPS61254856A (de)
CA (1) CA1249534A (de)
DE (1) DE3675684D1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4795701A (en) * 1985-07-17 1989-01-03 Allied Corporation Homogeneous polynucleotide displacement assay method kit and reagent complex
US4824776A (en) * 1985-07-25 1989-04-25 Molecular Biosystems, Inc. Method for increasing the sensitivity of nucleic acid hybridization assays
US5849478A (en) * 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
DE3639109A1 (de) * 1986-11-15 1988-05-19 Merck Patent Gmbh Verfahren zur bestimmung von nucleinsaeuren
WO1989004874A1 (en) * 1987-11-03 1989-06-01 Molecular Biosystems, Inc. Method and apparatus for fixation of biopolymers to a solid support by centrifugation
EP0385410B1 (de) * 1989-02-28 1996-10-02 Canon Kabushiki Kaisha Partiell doppelsträngiges Oligonukleotid und Verfahren zu seiner Bildung
EP0463036B1 (de) * 1989-03-10 1995-02-22 Amoco Corporation Immobilisierte oligonukleotidsonden und ihre verwendungen
EP0481999B1 (de) * 1989-03-10 1997-07-23 VYSIS, Inc. Interferenzverhinderung mit affinitätseinfangschemas
JPH03112496A (ja) * 1989-09-27 1991-05-14 Kikkoman Corp 物質の定量分析用標識剤
GB9009903D0 (en) * 1990-05-02 1990-06-27 Ici Plc Assay for specific nucleic acid sequences
US5451502A (en) * 1990-05-04 1995-09-19 Oncor, Inc. Restriction amplification assay
FR2666096B1 (fr) * 1990-08-21 1993-12-31 Sfri Sa Nouveau procede de dosage d'acide desoxyribonucleique present en position extracellulaire dans un milieu.
WO1992011390A1 (en) * 1990-12-17 1992-07-09 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
WO1992015708A1 (en) * 1991-02-27 1992-09-17 Amoco Corporation Methods for improving the sensitivity of hybridization assays
US5569582A (en) * 1991-07-15 1996-10-29 Institute Of Molecular Biology & Technology Rapid amplification and detection of nucleic acids
US5445933A (en) * 1992-09-15 1995-08-29 Boehringer Mannheim Corporation Strand displacement assay and complex useful therefor
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
GB9308411D0 (en) * 1993-04-23 1993-06-09 Celsis Ltd Detection of biological material
US5550040A (en) * 1993-06-23 1996-08-27 Hoffman-La Roche Inc. Method, reagents and kits for the detection of Neisseria gonorrhoeae
US6268133B1 (en) 1997-06-25 2001-07-31 Invitrogen Corporation Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
EP1092047B1 (de) * 1998-07-02 2009-08-26 Gen-Probe Incorporated Molekulare fackeln
EP1829856A3 (de) 1998-11-12 2009-02-25 Invitrogen Corporation Transfektionsreagenzien
US6514700B1 (en) * 1999-04-30 2003-02-04 Aclara Biosciences, Inc. Nucleic acid detection using degradation of a tagged sequence
US20040067498A1 (en) * 2000-04-28 2004-04-08 Ahmed Chenna Detection of nucleic acid sequences by cleavage and separation of tag-containing structures
CN1166422C (zh) * 2001-11-05 2004-09-15 北京源德生物医学工程股份有限公司 用于体外高能聚焦超声波治疗机的坐位架
US20050053939A1 (en) * 2001-11-09 2005-03-10 Ahmed Chenna Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
DE102010025161A1 (de) * 2010-06-25 2011-12-29 Qiagen Gmbh Verfahren zum Nachweis von Ribonukleinsäuren
WO2012092540A1 (en) * 2010-12-30 2012-07-05 Ibis Biosciences, Inc. Methods for the synthesis of polyadenylic acid
US9138064B2 (en) 2013-01-18 2015-09-22 Fxi, Inc. Mattress with combination of pressure redistribution and internal air flow guides
WO2014165007A1 (en) * 2013-03-12 2014-10-09 Promega Corporation Luminescent detection of inorganic phosphate and coupled reactions
EP3169310A1 (de) 2014-07-15 2017-05-24 Life Technologies Corporation Zusammensetzungen mit lipidaggregaten und verfahren zur effizienten bereitstellung von molekülen an zellen

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3933592A (en) * 1965-02-17 1976-01-20 Hazleton Laboratories, Incorporated Method of detecting living microorganisms
US3423290A (en) * 1966-08-03 1969-01-21 Nasa Lyophilized reaction mixtures
IL49685A (en) * 1976-05-31 1978-10-31 Technion Res & Dev Foundation Specific binding assay method for determining the concentration of materials and reagent means therefor
SE428379B (sv) * 1978-05-31 1983-06-27 Lkb Produkter Ab Bioluminiscens bestemning av atp och reagens herfor
DE2828658C3 (de) * 1978-06-29 1981-10-22 Lkb-Produkter Ab, Stockholm Verfahren zur photometrischen Bestimmung der Untereinheit B der Creatinkinase und Reagens hierfür
DE2834704A1 (de) * 1978-08-08 1980-02-21 Boehringer Mannheim Gmbh Verfahren zur quantitativen enzymatischen bestimmung von adp
DE2908054A1 (de) * 1979-03-02 1980-09-11 Boehringer Mannheim Gmbh Verfahren und reagenz zur bestimmung der creatinkinase
US4446231A (en) * 1979-10-03 1984-05-01 Self Colin H Immunoassay using an amplified cyclic detection system
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
CA1223831A (en) * 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
GB8306426D0 (en) * 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
GB8311905D0 (en) * 1983-04-29 1983-06-02 Cox R A Isolation and detection of nucleotide sequence
CA1254114A (en) * 1984-05-07 1989-05-16 Allied Corporation Displacement polynucleotide assay employing recombination protein and diagnostic kit

Also Published As

Publication number Publication date
CA1249534A (en) 1989-01-31
US4767699A (en) 1988-08-30
EP0200057A3 (en) 1987-02-25
EP0200057B1 (de) 1990-11-22
EP0200057A2 (de) 1986-11-05
JPS61254856A (ja) 1986-11-12

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Legal Events

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8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee