ATE218140T1 - Isolierung von nukleinsäuren - Google Patents

Isolierung von nukleinsäuren

Info

Publication number
ATE218140T1
ATE218140T1 AT98957019T AT98957019T ATE218140T1 AT E218140 T1 ATE218140 T1 AT E218140T1 AT 98957019 T AT98957019 T AT 98957019T AT 98957019 T AT98957019 T AT 98957019T AT E218140 T1 ATE218140 T1 AT E218140T1
Authority
AT
Austria
Prior art keywords
nucleic acids
beads
isolation
solid phase
activated
Prior art date
Application number
AT98957019T
Other languages
English (en)
Inventor
Matthew John Baker
Original Assignee
Dna Res Innovations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26312726&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE218140(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9725839.6A external-priority patent/GB9725839D0/en
Priority claimed from GBGB9815541.9A external-priority patent/GB9815541D0/en
Application filed by Dna Res Innovations Ltd filed Critical Dna Res Innovations Ltd
Application granted granted Critical
Publication of ATE218140T1 publication Critical patent/ATE218140T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
AT98957019T 1997-12-06 1998-12-04 Isolierung von nukleinsäuren ATE218140T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9725839.6A GB9725839D0 (en) 1997-12-06 1997-12-06 Isolation of nucleic acids
GBGB9815541.9A GB9815541D0 (en) 1998-07-17 1998-07-17 Isolation of nucleic acids
PCT/GB1998/003602 WO1999029703A2 (en) 1997-12-06 1998-12-04 Isolation of nucleic acids

Publications (1)

Publication Number Publication Date
ATE218140T1 true ATE218140T1 (de) 2002-06-15

Family

ID=26312726

Family Applications (2)

Application Number Title Priority Date Filing Date
AT98957019T ATE218140T1 (de) 1997-12-06 1998-12-04 Isolierung von nukleinsäuren
AT02003403T ATE386044T1 (de) 1997-12-06 1998-12-04 Isolierung von nukleinsäuren

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT02003403T ATE386044T1 (de) 1997-12-06 1998-12-04 Isolierung von nukleinsäuren

Country Status (16)

Country Link
US (1) US20030130499A1 (de)
EP (2) EP1036082B1 (de)
JP (2) JP2004501054A (de)
KR (2) KR20050088164A (de)
CN (1) CN1230440C (de)
AT (2) ATE218140T1 (de)
AU (1) AU755342B2 (de)
BR (1) BR9815569A (de)
CA (1) CA2318306A1 (de)
DE (2) DE69839133T2 (de)
DK (1) DK1036082T3 (de)
ES (2) ES2301581T3 (de)
HK (1) HK1034520A1 (de)
NO (1) NO315323B1 (de)
PT (1) PT1036082E (de)
WO (1) WO1999029703A2 (de)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
EP1171584A1 (de) * 1999-04-21 2002-01-16 Annovis, Inc. Kit für die magnetische dns-extraktion aus pflanzen
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
SE9904539D0 (sv) * 1999-12-10 1999-12-10 Alphahelix Ab Method and device for the handling of samples and reagents
JP2001221721A (ja) * 2000-02-09 2001-08-17 Sapporo Imuno Diagnostic Laboratory:Kk 乾燥濾紙便による遺伝子診断
DE10006590B4 (de) * 2000-02-11 2007-10-18 Qiagen North American Holdings, Inc. Verwendung funktionalisierter Membranen bzw. Matrizes zur Aufreinigung von Nukleinsäuren sowie entsprechende Verfahren
CA2433746A1 (en) * 2001-01-09 2002-07-18 Whitehead Institute For Biomedical Research Methods and reagents for the isolation of nucleic acids
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
GB0212825D0 (en) * 2002-05-31 2002-07-10 Dna Res Innovations Ltd Methods compositions and kits for cell separation
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
CN100395257C (zh) * 2003-05-08 2008-06-18 慈溪市中鼎生物技术有限公司 钾离子酸性水溶液和利用这种溶液的dna提取方法和试剂盒
JP4996248B2 (ja) 2003-07-31 2012-08-08 ハンディーラブ インコーポレイテッド 粒子含有サンプルの処理
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2005108620A2 (en) * 2004-05-03 2005-11-17 Handylab, Inc. Processing polynucleotide-containing samples
CA2566866A1 (en) * 2004-05-11 2006-03-09 Wyeth Novel polynucleotides related to oligonucleotide arrays to monitor gene expression
WO2006004611A2 (en) * 2004-06-25 2006-01-12 Invitrogen Corporation Separation of nucleic acid
AU2005330267A1 (en) 2004-07-28 2006-10-12 Canon U.S. Life Sciences, Inc. Methods for monitoring genomic DNA of organisms
EP1776458A2 (de) * 2004-07-30 2007-04-25 Agencourt Bioscience Corporation Verfahren zur isolierung von nukleinsäuren mit hilfe von festphasenträgern mit multifunktionalen gruppen
WO2006017428A2 (en) * 2004-08-03 2006-02-16 Becton, Dickinson And Company Use of magnetic material to direct isolation of compounds and fractionation of multipart samples
KR100647306B1 (ko) * 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 pH에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
US7964380B2 (en) 2005-01-21 2011-06-21 Argylia Technologies Nanoparticles for manipulation of biopolymers and methods of thereof
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
KR100647315B1 (ko) * 2005-02-02 2006-11-23 삼성전자주식회사 실란화된 고상 물질을 이용한 핵산의 분리 및 증폭 방법
JP4982387B2 (ja) * 2005-02-18 2012-07-25 キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッド 微生物のゲノムdnaを同定するデバイスおよび方法
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
KR100668337B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은pH 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
CN101300352B (zh) 2005-09-01 2013-03-20 佳能美国生命科学公司 检测、分析和鉴定基因组dna的方法和分子诊断装置
GB0522193D0 (en) 2005-10-31 2005-12-07 Axis Shield Asa Method
WO2007066665A1 (ja) * 2005-12-05 2007-06-14 Universal Bio Research Co., Ltd. cRNAの調製方法
ES2692380T3 (es) 2006-03-24 2018-12-03 Handylab, Inc. Método para realizar PCR con un cartucho con varias pistas
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
KR100785010B1 (ko) * 2006-04-06 2007-12-11 삼성전자주식회사 수소 결합을 이용하여 고체 지지체의 친수성 표면 상에서핵산 정제 방법 및 장치
WO2008054514A2 (en) 2006-04-21 2008-05-08 Wyeth Differential expression profiling analysis of cell culture phenotypes and the uses thereof
US7608399B2 (en) 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
KR100813264B1 (ko) * 2006-08-21 2008-03-13 삼성전자주식회사 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법
KR100837401B1 (ko) * 2006-08-21 2008-06-12 삼성전자주식회사 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 분리하는 방법,상기 분리된 핵산을 주형으로 하여핵산을 증폭하는 방법 및 상기 고체 지지체를 포함하는핵산 분리 및 증폭 장치
US20080070282A1 (en) 2006-08-21 2008-03-20 Samsung Electronics Co., Ltd. Method and device for obtaining or amplifying nucleic acid from a cell using a nonplanar solid substrate
KR100813265B1 (ko) * 2006-08-21 2008-03-13 삼성전자주식회사 비평면 형상의 고체 지지체를 이용하여 미생물로부터핵산을 증폭하는 방법
US8158411B2 (en) 2006-08-21 2012-04-17 Samsung Electronics Co., Ltd. Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same
WO2008035991A2 (en) * 2006-09-19 2008-03-27 Michael Ronald Cook A nucleic acid extraction method
GB2445441B (en) 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
WO2008061165A2 (en) * 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
US8999636B2 (en) * 2007-01-08 2015-04-07 Toxic Report Llc Reaction chamber
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
WO2008157299A2 (en) * 2007-06-15 2008-12-24 Wyeth Differential expression profiling analysis of cell culture phenotypes and uses thereof
WO2009009127A2 (en) * 2007-07-12 2009-01-15 U.S. Genomics, Inc. Method and system for transferring and/or concentrating a sample
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
AU2008276211B2 (en) 2007-07-13 2015-01-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8399190B2 (en) 2009-09-21 2013-03-19 Akonni Biosystems Magnetic lysis method and device
US10125388B2 (en) 2007-10-31 2018-11-13 Akonni Biosystems, Inc. Integrated sample processing system
WO2009086215A2 (en) * 2007-12-21 2009-07-09 Wyeth Pathway analysis of cell culture phenotypes and uses thereof
WO2009117167A1 (en) 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
GB0814570D0 (en) 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
KR101015502B1 (ko) 2008-09-09 2011-02-22 삼성전자주식회사 제1 pH에서 양전하 및 제2 pH에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법
US8283165B2 (en) 2008-09-12 2012-10-09 Genvault Corporation Matrices and media for storage and stabilization of biomolecules
US8361716B2 (en) * 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
US20100204462A1 (en) 2008-10-13 2010-08-12 Thomas Walter Pipette tip with separation material
DE102008063001A1 (de) 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
DE102008063003A1 (de) 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
JP5766178B2 (ja) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴThe University Of Chicago SlipChip装置および方法
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
EP2256195A1 (de) * 2009-05-12 2010-12-01 Qiagen GmbH Nukleinsäureaufreinigungsverfahren
US8415467B2 (en) * 2009-12-14 2013-04-09 Betty Wu Method and materials for separating nucleic acid materials
KR101878749B1 (ko) 2010-03-05 2018-07-17 삼성전자주식회사 표적 세포의 분리 방법 및 키트
BR112013001647A2 (pt) 2010-07-23 2016-05-24 Beckman Coulter Inc sistema e método contendo unidades analíticas
ES2769028T3 (es) 2011-04-15 2020-06-24 Becton Dickinson Co Termociclador microfluídico de barrido en tiempo real
WO2013004710A2 (en) * 2011-07-04 2013-01-10 Qiagen Gmbh Reagent usable for the isolation and/or purification of nucleic acids
CN113388605A (zh) 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
DK3273253T3 (da) 2011-09-30 2020-10-12 Becton Dickinson Co Forenet reagensstrimmel
WO2013062476A1 (en) 2011-10-27 2013-05-02 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
CN104040238B (zh) 2011-11-04 2017-06-27 汉迪拉布公司 多核苷酸样品制备装置
WO2013070740A1 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Aliquotter system and workflow
ES2729283T3 (es) 2011-11-07 2019-10-31 Beckman Coulter Inc Sistema de centrífuga y flujo de trabajo
WO2013070756A2 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. System and method for processing samples
BR112014011048A2 (pt) 2011-11-07 2017-05-02 Beckman Coulter Inc braço robótico
EP2776844B1 (de) 2011-11-07 2020-09-30 Beckman Coulter, Inc. Probenbehälterdetektion
KR20140091032A (ko) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 검체 수송 시스템의 자기 감쇠
CN107881219B (zh) 2012-02-03 2021-09-10 贝克顿·迪金森公司 用于分子诊断测试分配和测试之间兼容性确定的外部文件
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
CA2870999A1 (en) 2012-04-20 2013-10-24 SlipChip, LLC Fluidic devices and systems for sample preparation or autonomous analysis
WO2013159116A1 (en) 2012-04-20 2013-10-24 University Of Chicago Fluidic devices for biospecimen preservation
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
WO2014035090A1 (ko) 2012-08-28 2014-03-06 주식회사 바이오큐브시스템 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
US9719082B2 (en) * 2013-10-31 2017-08-01 General Electric Company Substrates and associated methods for elution of nucleic acids
WO2015199423A1 (ko) * 2014-06-24 2015-12-30 (주)바이오니아 자성 입자를 이용한 핵산 분리 방법
CN104513819B (zh) * 2014-12-17 2020-05-05 吉林大学 一种选择性提取dna的方法
EP3885438A1 (de) 2015-06-10 2021-09-29 QIAGEN GmbH Verfahren zur isolierung extrazellulärer nukleinsäuren mittels anionenaustauschpartikeln
KR101696549B1 (ko) * 2015-07-31 2017-01-13 전북대학교산학협력단 수소이온농도 대응 검체 스왑 장치
KR102190922B1 (ko) * 2015-09-01 2020-12-14 삼성전자주식회사 핵산 분리 방법
JP7163186B2 (ja) 2016-03-19 2022-10-31 キアゲン ゲーエムベーハー Rnaの安定化
US11161107B2 (en) 2016-05-25 2021-11-02 Integrated Micro-Chromatography Systems, Inc. Dispersive pipette extraction system for purification of large biomolecules
CN106520522A (zh) * 2016-12-06 2017-03-22 厦门华厦学院 一种dna萃取装置及方法
EP3502274A1 (de) * 2017-12-22 2019-06-26 Attomol GmbH Probenträger und verfahren zu dessen herstellung
CN108660074B (zh) * 2018-05-28 2022-04-29 嘉兴市艾科诺生物科技有限公司 核酸提取pcr扩增检测一体化解决方案
WO2020153248A1 (ja) * 2019-01-25 2020-07-30 積水化学工業株式会社 核酸の単離方法、核酸の単離キット、及び検査チップ
JP2020124129A (ja) * 2019-02-01 2020-08-20 積水化学工業株式会社 核酸の単離方法及び核酸単離用デバイス
CN110129314B (zh) * 2019-04-24 2021-04-13 合肥欧创基因生物科技有限公司 一种氨基酸缓冲液及血浆游离dna提取试剂盒
CN114845957A (zh) * 2019-09-18 2022-08-02 南科征途有限公司 用于按尺寸富集和分离核酸的设备系统和方法
US20230028205A1 (en) 2019-12-16 2023-01-26 Qiagen Gmbh Enrichment method
WO2022263500A1 (en) 2021-06-17 2022-12-22 Qiagen Gmbh Method for isolating non-vesicular mirna

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3797202A (en) * 1971-08-27 1974-03-19 Gen Electric Microporous/non-porous composite membranes
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
US4843012A (en) * 1986-09-17 1989-06-27 Genetics Institute, Inc. Novel composition for nucleic acid purification
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
JPH01125395A (ja) * 1987-07-29 1989-05-17 Life Technol Inc 核酸浦獲試薬
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US4908318A (en) * 1987-09-04 1990-03-13 Integrated Genetics, Inc. Nucleic acid extraction methods
US4923978A (en) * 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5124444A (en) * 1989-07-24 1992-06-23 Microprobe Corporation Lactam-containing compositions and methods useful for the extraction of nucleic acids
US5128247A (en) * 1989-08-14 1992-07-07 Board Of Regents, The University Of Texas System Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources
US5433847A (en) * 1989-11-01 1995-07-18 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Radial flow chromatography
US5641628A (en) * 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
IT1240870B (it) * 1990-02-14 1993-12-17 Talent Procedimento per l'estrazione e la purificazione di dna genomico umano
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5654179A (en) * 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5204246A (en) * 1990-12-26 1993-04-20 Pioneer Hi-Bred International, Inc. Dna isolation method
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
CA2187338A1 (en) * 1994-04-08 1995-10-19 Jin-Yan Tang Purification of oligodeoxynucleotide phosphorothioates using anion exchange chromatography
US5622822A (en) * 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) * 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) * 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
US5631146A (en) * 1995-01-19 1997-05-20 The General Hospital Corporation DNA aptamers and catalysts that bind adenosine or adenosine-5'-phosphates and methods for isolation thereof
US5612473A (en) * 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
SE9600590D0 (sv) * 1996-02-19 1996-02-19 Pharmacia Biotech Ab Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
US5916746A (en) * 1996-05-09 1999-06-29 Kirkegaard & Perry Laboratories, Inc. Formazan-based immunoassay
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
JP3793287B2 (ja) * 1996-08-22 2006-07-05 ペンタックス株式会社 プラスミドdnaの単離方法
CA2214495C (en) * 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
US6060246A (en) * 1996-11-15 2000-05-09 Avi Biopharma, Inc. Reagent and method for isolation and detection of selected nucleic acid sequences
US5770712A (en) * 1997-03-14 1998-06-23 Virginia Tech Intellectual Properties, Inc. Crosslinked hydrogel beads from chitosan
EP0897978A3 (de) * 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirkoniumoxyd und verwandten Verbindungen zur Reinigung von Nukleinsäuren
JP4304348B2 (ja) * 1997-09-22 2009-07-29 独立行政法人理化学研究所 Dnaの単離方法
DE19743518A1 (de) * 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
WO2000044928A2 (en) * 1999-01-27 2000-08-03 Halaka Folim G Materials and methods for the purification of polyelectrolytes
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids

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EP1234832A2 (de) 2002-08-28
NO20002540L (no) 2000-07-07
ATE386044T1 (de) 2008-03-15
JP2010162037A (ja) 2010-07-29
DE69839133T2 (de) 2009-02-05
NO315323B1 (no) 2003-08-18
EP1234832B1 (de) 2008-02-13
EP1036082B1 (de) 2002-05-29
US20030130499A1 (en) 2003-07-10
KR20050088164A (ko) 2005-09-01
WO1999029703A3 (en) 1999-08-26
ES2301581T3 (es) 2008-07-01
EP1234832A3 (de) 2003-08-13
CN1281462A (zh) 2001-01-24
NO20002540D0 (no) 2000-05-16
ES2177093T3 (es) 2002-12-01
DK1036082T3 (da) 2002-08-12
PT1036082E (pt) 2002-10-31
BR9815569A (pt) 2001-10-09
DE69805649D1 (de) 2002-07-04
JP2004501054A (ja) 2004-01-15
KR20010032806A (ko) 2001-04-25
DE69839133D1 (de) 2008-03-27
HK1034520A1 (en) 2001-10-26
EP1036082A2 (de) 2000-09-20
AU755342B2 (en) 2002-12-12
CN1230440C (zh) 2005-12-07
DE69805649T2 (de) 2002-12-05
AU1344799A (en) 1999-06-28
CA2318306A1 (en) 1999-06-17
WO1999029703A2 (en) 1999-06-17

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