DE58889T1 - Plasmid pcg1. - Google Patents

Plasmid pcg1.

Info

Publication number
DE58889T1
DE58889T1 DE198282101018T DE82101018T DE58889T1 DE 58889 T1 DE58889 T1 DE 58889T1 DE 198282101018 T DE198282101018 T DE 198282101018T DE 82101018 T DE82101018 T DE 82101018T DE 58889 T1 DE58889 T1 DE 58889T1
Authority
DE
Germany
Prior art keywords
plasmid
pcg1
plasmid pcg1
corynebacterium glutamicum
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282101018T
Other languages
German (de)
English (en)
Inventor
Akira Kawasaki-Shi Kanagawa-Ken Furuya
Ryoichi Machida-Shi Tokyo Katsumata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Publication of DE58889T1 publication Critical patent/DE58889T1/de
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE198282101018T 1981-02-12 1982-02-11 Plasmid pcg1. Pending DE58889T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56018101A JPS57134500A (en) 1981-02-12 1981-02-12 Plasmid pcg1

Publications (1)

Publication Number Publication Date
DE58889T1 true DE58889T1 (de) 1983-04-14

Family

ID=11962230

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198282101018T Pending DE58889T1 (de) 1981-02-12 1982-02-11 Plasmid pcg1.
DE8282101018T Expired DE3274561D1 (en) 1981-02-12 1982-02-11 Plasmid pcg1

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8282101018T Expired DE3274561D1 (en) 1981-02-12 1982-02-11 Plasmid pcg1

Country Status (5)

Country Link
US (1) US4617267A (OSRAM)
EP (1) EP0058889B1 (OSRAM)
JP (1) JPS57134500A (OSRAM)
CA (1) CA1196588A (OSRAM)
DE (2) DE58889T1 (OSRAM)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4778762A (en) * 1980-04-17 1988-10-18 Ajinomoto Company Incorporated Plasmid
JPS5877895A (ja) * 1981-11-02 1983-05-11 Ajinomoto Co Inc プラスミドphm1519
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
JPS58126789A (ja) * 1981-12-29 1983-07-28 Kyowa Hakko Kogyo Co Ltd レースレオニンの製造法
US4559308A (en) * 1983-01-27 1985-12-17 Stauffer Chemical Co. Corynebacterium plasmid and vector
JPS59156292A (ja) * 1983-02-17 1984-09-05 Kyowa Hakko Kogyo Co Ltd トリプトフアンの製造法
JPS59156294A (ja) * 1983-02-17 1984-09-05 Kyowa Hakko Kogyo Co Ltd ヒスチジンの製造法
JPS6066989A (ja) * 1983-09-24 1985-04-17 Kyowa Hakko Kogyo Co Ltd L−アルギニンの製造法
JPS59196098A (ja) * 1983-04-23 1984-11-07 Ajinomoto Co Inc 発酵法によるl−トリプトフアンの製造法
US4649119A (en) * 1983-04-28 1987-03-10 Massachusetts Institute Of Technology Cloning systems for corynebacterium
JPH0783714B2 (ja) * 1983-08-29 1995-09-13 味の素株式会社 発酵法によるl―アミノ酸の製造法
JPH06102029B2 (ja) * 1984-07-31 1994-12-14 味の素株式会社 L−トリプトフアンの製造法
GB2165546B (en) * 1984-08-21 1989-05-17 Asahi Chemical Ind A plasmid containing a gene for tetracycline resistance and dna fragments derived therefrom
EP0183175B1 (en) * 1984-11-20 1990-03-14 Ajinomoto Co., Inc. Coryneform bacteria carrying recombinant dna and a process for producing aromatic amino acids using said bacteria
JP2967996B2 (ja) 1989-06-06 1999-10-25 協和醗酵工業株式会社 L―トリプトファンの製造法
KR0161531B1 (ko) * 1990-03-08 1998-11-16 하야시바라 겐 락토수크로오스 고함유 분말의 제조방법과 그 분말의 용도
JP3078312B2 (ja) * 1990-11-22 2000-08-21 協和醗酵工業株式会社 物質の製造法
JPH06225776A (ja) * 1992-12-07 1994-08-16 Kyowa Hakko Kogyo Co Ltd リボフラビンの製造法
DE69409895T2 (de) * 1993-01-13 1998-12-24 Ajinomoto Co., Inc., Tokio/Tokyo Zelloberflächenprotein aus brevibakterium lactofermentum
JP4184606B2 (ja) * 1998-09-04 2008-11-19 協和醗酵工業株式会社 新規遺伝子
CA2383875A1 (en) 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production
DE1246922T1 (de) 1999-07-01 2003-03-20 Basf Ag Für proteine des phosphoenolpyruvat:zucker phosphotransferase systems kodierende gene aus corynebacterium glutamicum
TR200103854T2 (tr) 1999-07-01 2002-06-21 Basf Aktiengesellschaft Corynebacterium glutamicum gen kodlayıcı fosfonolpiruvat: şeker fosfotransferaz sistem proteini
PL341895A1 (en) 1999-08-12 2001-02-26 Ajinomoto Kk Plasmide autonomously replicable in corynebacter bacteria
US6927046B1 (en) 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
JP4469568B2 (ja) 2003-07-09 2010-05-26 三菱化学株式会社 有機酸の製造方法
BRPI0413403A (pt) 2003-08-28 2006-10-17 Mitsubishi Chem Corp método para produzir ácido succìnico
JP5023701B2 (ja) 2004-05-20 2012-09-12 味の素株式会社 コハク酸生産菌及びコハク酸の製造方法
JP5572279B2 (ja) * 2004-05-20 2014-08-13 味の素株式会社 コハク酸生産菌及びコハク酸の製造方法
JP2008522609A (ja) * 2004-12-06 2008-07-03 セージ バイオサイエンセズ インコーポレイテッド 反芻動物の腸へ生物活性化合物を供給するための細菌の培養方法
BR122020021800B1 (pt) 2004-12-28 2021-05-04 Ajinomoto Co., Inc Método para produzir ácido l-glutâmico
EP1947190B1 (en) 2005-10-18 2017-11-29 Ajinomoto Co., Inc. Process for production of succinic acid
ES2382207T3 (es) 2005-12-27 2012-06-06 Kyowa Hakko Bio Co., Ltd. Procedimiento para la producción de L-glutamina
EP1995308B1 (en) 2006-02-24 2014-07-30 Mitsubishi Chemical Corporation Bacterium capable of producing organic acid, and method for production of organic acid
JP2010130899A (ja) 2007-03-14 2010-06-17 Ajinomoto Co Inc L−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法
EP2149607B1 (en) 2007-04-10 2017-12-20 Ajinomoto Co., Inc. Method for production of succinic acid
WO2008133131A1 (ja) 2007-04-16 2008-11-06 Ajinomoto Co., Inc. 有機酸の製造方法
WO2009025363A1 (ja) 2007-08-23 2009-02-26 Mitsubishi Chemical Corporation コハク酸の製造方法
EP2192170B1 (en) 2007-09-04 2017-02-15 Ajinomoto Co., Inc. Amino acid-producing microorganism and method of producing amino acid
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
WO2010024267A1 (ja) 2008-09-01 2010-03-04 国立大学法人信州大学 有用物質の製造法
JPWO2010079594A1 (ja) 2009-01-07 2012-06-21 三菱化学株式会社 ステロール側鎖切断酵素蛋白質およびその利用
WO2010095642A1 (ja) 2009-02-18 2010-08-26 国立大学法人信州大学 有用物質の製造方法
CA2755310A1 (en) 2009-03-30 2010-10-07 Research Institute Of Innovative Technology For The Earth Coryneform bacterium transformant and process for producing isobutanol using the same
JP2012223091A (ja) 2009-08-25 2012-11-15 Ajinomoto Co Inc L−アミノ酸の製造法
CN102834515B (zh) 2010-08-31 2016-08-03 株式会社Api 新的水解酶蛋白
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KR101972556B1 (ko) 2010-11-10 2019-04-25 그린 케미칼즈 가부시키가이샤 코리네형 세균 형질 전환체 및 그것을 사용하는 페놀의 제조 방법
CA2830373A1 (en) 2011-03-18 2012-09-27 Mitsubishi Chemical Corporation Method for producing polymer, method for producing organic acid, and organic acid-producing microorganism
EP2749638A4 (en) 2011-08-22 2015-04-22 Res Inst Innovative Tech Earth MEANS FOR TRANSFORMING CORYNEFORMER BACTERIA AND PROCESS FOR VALIN PRODUCTION THEREWITH
EP2772546B1 (en) 2011-10-25 2019-05-15 Ajinomoto Co., Inc. Secretion production method for protein
CN103946372B (zh) 2011-11-02 2016-06-08 味之素株式会社 用于分泌产生蛋白质的方法
KR101653555B1 (ko) 2011-11-02 2016-09-02 아지노모토 가부시키가이샤 단백질의 분비 생산 방법
CN103930557A (zh) 2011-11-11 2014-07-16 味之素株式会社 利用发酵法制造目标物质的方法
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WO2014126260A1 (ja) 2013-02-18 2014-08-21 味の素株式会社 タンパク質の沈殿による製造方法
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JP5831669B2 (ja) 2013-05-13 2015-12-09 味の素株式会社 L−アミノ酸の製造法
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
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BR112016008830B1 (pt) 2013-10-23 2023-02-23 Ajinomoto Co., Inc Método para produzir uma substância alvo
EP3101130B1 (en) 2014-01-31 2020-05-06 Ajinomoto Co., Inc. Mutant glutamate-cysteine ligase and method for manufacturing gamma-glutamyl-valyl-glycine
JP2017216881A (ja) 2014-12-26 2017-12-14 味の素株式会社 ジカルボン酸の製造方法
EP3298153A1 (en) 2015-05-19 2018-03-28 Lucite International UK Limited Process for the biological production of methacrylic acid and derivatives thereof
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EP3530748B1 (en) 2016-10-21 2021-06-02 Ajinomoto Co., Inc. Method for the secretory production proteins
CN109952380B (zh) 2016-10-26 2023-07-14 味之素株式会社 用于生产目标物质的方法
WO2018079685A1 (en) 2016-10-26 2018-05-03 Ajinomoto Co., Inc. Method for producing objective substance
WO2018079683A1 (en) 2016-10-26 2018-05-03 Ajinomoto Co., Inc. Method for producing objective substance
WO2018079686A1 (en) 2016-10-26 2018-05-03 Ajinomoto Co., Inc. Method for producing l-methionine or metabolites requiring s-adenosylmethionine for synthesis
JP2019536449A (ja) 2016-10-26 2019-12-19 味の素株式会社 目的物質の製造方法
WO2018079705A1 (en) 2016-10-27 2018-05-03 Ajinomoto Co., Inc. Method for producing aldehyde
GB201619827D0 (en) 2016-11-23 2017-01-04 Lucite Int Uk Ltd Process for the production of methyl methacrylate
WO2018179834A1 (en) 2017-03-28 2018-10-04 Ajinomoto Co., Inc. Method for producing rna
JP7066977B2 (ja) 2017-04-03 2022-05-16 味の素株式会社 L-アミノ酸の製造法
WO2018190398A1 (ja) 2017-04-13 2018-10-18 協和発酵バイオ株式会社 テアニンの製造方法
US10858676B2 (en) 2017-05-22 2020-12-08 Ajinomoto Co., Inc. Method for producing objective substance
AU2018334768B2 (en) 2017-09-25 2024-08-08 Ajinomoto Co., Inc. Protein production method and disaccharide production method
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
US11680279B2 (en) 2017-11-29 2023-06-20 Ajinomoto Co., Inc. Method for producing objective substance
EP3732298A1 (en) 2017-12-26 2020-11-04 Ajinomoto Co., Inc. Method of producing glycine by fermentation
BR112020016770A2 (pt) 2018-02-20 2020-12-15 Ajinomoto Co., Inc. Método para induzir silenciamento de rna e para produzir uma composição para induzir silenciamento de rna em um organismo-alvo, e, composição para induzir silenciamento de rna em um organismo-alvo
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WO2020027251A1 (en) 2018-08-03 2020-02-06 Ajinomoto Co., Inc. Method for producing objective substance
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EP3835412A4 (en) 2018-08-10 2022-05-11 Kyowa Hakko Bio Co., Ltd. Microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid
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GB2621337A (en) 2022-08-08 2024-02-14 Mitsubishi Chemical Uk Ltd Process for the biological production of methacrylic acid and derivatives thereof
WO2024122636A1 (ja) 2022-12-09 2024-06-13 キリンホールディングス株式会社 ポリエチレンテレフタレート分解活性を有する蛋白質及びポリエチレンテレフタレートを分解する方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3003925A (en) * 1956-05-17 1961-10-10 Kyowa Hakko Kogyo Kk Method of producing l-glutamic acid by fermentation
US3002889A (en) * 1960-06-20 1961-10-03 Kyowa Hakko Kogyo Kk Method of producing l-glutamic acid
US3220929A (en) * 1964-02-10 1965-11-30 Kyowa Hakko Kogyo Kk Microbiological production of amino acid by reductive amination
AU542264B2 (en) * 1979-06-01 1985-02-14 G.D. Searle & Co. Plasmid vectors
JPS57183799A (en) * 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid

Also Published As

Publication number Publication date
EP0058889B1 (en) 1986-12-03
EP0058889A1 (en) 1982-09-01
DE3274561D1 (en) 1987-01-15
CA1196588A (en) 1985-11-12
JPS57134500A (en) 1982-08-19
JPH0112477B2 (OSRAM) 1989-03-01
US4617267A (en) 1986-10-14

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