DE58889T1 - Plasmid pcg1. - Google Patents

Plasmid pcg1.

Info

Publication number
DE58889T1
DE58889T1 DE198282101018T DE82101018T DE58889T1 DE 58889 T1 DE58889 T1 DE 58889T1 DE 198282101018 T DE198282101018 T DE 198282101018T DE 82101018 T DE82101018 T DE 82101018T DE 58889 T1 DE58889 T1 DE 58889T1
Authority
DE
Germany
Prior art keywords
plasmid
pcg1
plasmid pcg1
corynebacterium glutamicum
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282101018T
Other languages
German (de)
English (en)
Inventor
Akira Kawasaki-Shi Kanagawa-Ken Furuya
Ryoichi Machida-Shi Tokyo Katsumata
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Publication of DE58889T1 publication Critical patent/DE58889T1/de
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE198282101018T 1981-02-12 1982-02-11 Plasmid pcg1. Pending DE58889T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56018101A JPS57134500A (en) 1981-02-12 1981-02-12 Plasmid pcg1

Publications (1)

Publication Number Publication Date
DE58889T1 true DE58889T1 (de) 1983-04-14

Family

ID=11962230

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198282101018T Pending DE58889T1 (de) 1981-02-12 1982-02-11 Plasmid pcg1.
DE8282101018T Expired DE3274561D1 (en) 1981-02-12 1982-02-11 Plasmid pcg1

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8282101018T Expired DE3274561D1 (en) 1981-02-12 1982-02-11 Plasmid pcg1

Country Status (5)

Country Link
US (1) US4617267A (OSRAM)
EP (1) EP0058889B1 (OSRAM)
JP (1) JPS57134500A (OSRAM)
CA (1) CA1196588A (OSRAM)
DE (2) DE58889T1 (OSRAM)

Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4778762A (en) * 1980-04-17 1988-10-18 Ajinomoto Company Incorporated Plasmid
JPS5877895A (ja) * 1981-11-02 1983-05-11 Ajinomoto Co Inc プラスミドphm1519
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
JPS58126789A (ja) * 1981-12-29 1983-07-28 Kyowa Hakko Kogyo Co Ltd レースレオニンの製造法
US4559308A (en) * 1983-01-27 1985-12-17 Stauffer Chemical Co. Corynebacterium plasmid and vector
JPS59156292A (ja) * 1983-02-17 1984-09-05 Kyowa Hakko Kogyo Co Ltd トリプトフアンの製造法
JPS59156294A (ja) * 1983-02-17 1984-09-05 Kyowa Hakko Kogyo Co Ltd ヒスチジンの製造法
JPS6066989A (ja) * 1983-09-24 1985-04-17 Kyowa Hakko Kogyo Co Ltd L−アルギニンの製造法
JPS59196098A (ja) * 1983-04-23 1984-11-07 Ajinomoto Co Inc 発酵法によるl−トリプトフアンの製造法
US4649119A (en) * 1983-04-28 1987-03-10 Massachusetts Institute Of Technology Cloning systems for corynebacterium
JPH0783714B2 (ja) * 1983-08-29 1995-09-13 味の素株式会社 発酵法によるl―アミノ酸の製造法
JPH06102029B2 (ja) * 1984-07-31 1994-12-14 味の素株式会社 L−トリプトフアンの製造法
GB2165546B (en) * 1984-08-21 1989-05-17 Asahi Chemical Ind A plasmid containing a gene for tetracycline resistance and dna fragments derived therefrom
EP0183175B1 (en) * 1984-11-20 1990-03-14 Ajinomoto Co., Inc. Coryneform bacteria carrying recombinant dna and a process for producing aromatic amino acids using said bacteria
JP2967996B2 (ja) * 1989-06-06 1999-10-25 協和醗酵工業株式会社 L―トリプトファンの製造法
DE69111271T2 (de) * 1990-03-08 1996-02-22 Ensuiko Sugar Refining Verfahren zur Herstellung eines Pulvers/mit erhöhtem Gehalt an Lactosucrose und Verwendung dieses Pulvers.
JP3078312B2 (ja) * 1990-11-22 2000-08-21 協和醗酵工業株式会社 物質の製造法
JPH06225776A (ja) * 1992-12-07 1994-08-16 Kyowa Hakko Kogyo Co Ltd リボフラビンの製造法
US5547864A (en) * 1993-01-13 1996-08-20 Ajinomoto Co., Inc. Coryneform bacteria deficient in a cell surface protein
US6855516B1 (en) * 1998-09-04 2005-02-15 Kyowa Hakko Kogyo, Co., Ltd. Gene conferring lysozyme insensitivity to corynebacterium
EP2292763A1 (en) 1999-06-25 2011-03-09 Paik Kwang Industrial Co., Ltd. Corynebacterium glutamicum genes encoding proteins involved in carbon metabolism and energy production
EP1702980A1 (en) 1999-07-01 2006-09-20 Basf Aktiengesellschaft Corynebacterium glutamicum gene encoding Hpr of phosphoenolpyruvate:sugar phosphotransferase system
WO2001002583A2 (en) 1999-07-01 2001-01-11 Basf Aktiengesellschaft Orynebacterium glutamicum genes encoding phosphoenolpyruvate: sugar phosphotransferase system proteins
PL341895A1 (en) 1999-08-12 2001-02-26 Ajinomoto Kk Plasmide autonomously replicable in corynebacter bacteria
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
JP4469568B2 (ja) 2003-07-09 2010-05-26 三菱化学株式会社 有機酸の製造方法
BRPI0413403A (pt) 2003-08-28 2006-10-17 Mitsubishi Chem Corp método para produzir ácido succìnico
JP5572279B2 (ja) * 2004-05-20 2014-08-13 味の素株式会社 コハク酸生産菌及びコハク酸の製造方法
BRPI0510919A (pt) 2004-05-20 2008-05-20 Ajinomoto Kk bactéria produtora de ácido succìnico e processo para produzir ácido succìnico
BRPI0518845A2 (pt) * 2004-12-06 2008-12-09 Sage Biosciences Inc mÉtodo para aumentar a resistÊncia À inativaÇço ruminal de uma cepa de bactÉrias gram-positivas produtoras de lisina, suplemento alimentÍcio de lisina protegido no rémen, e, mÉtodos para aumentar o teor de lisina metabolicamente disponÍvel de uma raÇço alimentÍcia para ruminantes, e in vitro para avaliar a resistÊncia de uma cepa de bactÉrias produtoras de lisina para inativaÇço ruminal in vivo
KR101233739B1 (ko) 2004-12-28 2013-02-18 아지노모토 가부시키가이샤 L-글루탐산 생산 미생물 및 l-글루탐산의 제조방법
BRPI0617491B1 (pt) 2005-10-18 2021-04-27 Ajinomoto Co., Inc Processo para produção de ácido succínico
US8067211B2 (en) 2005-12-27 2011-11-29 Kyowa Hakko Bio Co., Ltd. Method for production of L-glutamine
EP1995308B1 (en) 2006-02-24 2014-07-30 Mitsubishi Chemical Corporation Bacterium capable of producing organic acid, and method for production of organic acid
JP2010130899A (ja) 2007-03-14 2010-06-17 Ajinomoto Co Inc L−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法
WO2008126896A1 (ja) 2007-04-10 2008-10-23 Ajinomoto Co., Inc. 有機酸の製造方法
EP2149608A4 (en) 2007-04-16 2013-02-20 Ajinomoto Kk PROCESS FOR PREPARING AN ORGANIC ACID
BRPI0815690A2 (pt) 2007-08-23 2014-10-14 Mitsubishi Chem Corp Métodos para produzir ácido succínico, e para produzir um polímero contendo ácido succínico.
CN101939412B (zh) 2007-09-04 2016-01-20 味之素株式会社 生产氨基酸的微生物以及氨基酸的生产方法
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
EP2330184B1 (en) 2008-09-01 2020-11-25 Shinshu University Process for producing a useful substance in coryneform bacteria
WO2010079594A1 (ja) 2009-01-07 2010-07-15 三菱化学株式会社 ステロール側鎖切断酵素蛋白質およびその利用
JPWO2010095642A1 (ja) 2009-02-18 2012-08-23 国立大学法人信州大学 有用物質の製造方法
CA2755310A1 (en) 2009-03-30 2010-10-07 Research Institute Of Innovative Technology For The Earth Coryneform bacterium transformant and process for producing isobutanol using the same
JP2012223091A (ja) 2009-08-25 2012-11-15 Ajinomoto Co Inc L−アミノ酸の製造法
SG187652A1 (en) 2010-08-31 2013-03-28 Api Corp Novel hydrolase protein
US9328361B2 (en) 2010-09-08 2016-05-03 Green Phenol Development Co., Ltd. Coryneform bacterium transformant and process for producing phenol using the same
JP5996434B6 (ja) 2010-11-10 2018-06-27 グリーンフェノール開発株式会社 コリネ型細菌形質転換体及びそれを用いるフェノールの製造方法
JP6032198B2 (ja) 2011-03-18 2016-11-24 三菱化学株式会社 ポリマーの製造方法、有機酸の製造方法及び有機酸生産菌
WO2013027709A1 (ja) 2011-08-22 2013-02-28 公益財団法人地球環境産業技術研究機構 コリネ型細菌形質転換体及びそれを用いるバリンの製造方法
JP6064912B2 (ja) 2011-10-25 2017-01-25 味の素株式会社 タンパク質の分泌生産法
EP2774982B1 (en) 2011-11-02 2017-06-21 Ajinomoto Co., Inc. Method for secreting and producing proteins
IN2014CN04039A (OSRAM) 2011-11-02 2015-10-23 Ajinomoto Kk
JPWO2013069634A1 (ja) 2011-11-11 2015-04-02 味の素株式会社 発酵法による目的物質の製造法
WO2013179711A1 (ja) 2012-05-29 2013-12-05 味の素株式会社 3-アセチルアミノ-4-ヒドロキシ安息香酸の製造方法
JP5673986B1 (ja) 2013-02-18 2015-02-18 味の素株式会社 タンパク質の沈殿による製造方法
RU2013119826A (ru) 2013-04-29 2014-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Коринеформная бактерия и способ получения гетерологичных гибридных белков
EP2868745B1 (en) 2013-05-13 2017-06-21 Ajinomoto Co., Inc. Method for manufacturing an L-amino acid
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
JP2016192903A (ja) 2013-09-17 2016-11-17 味の素株式会社 海藻由来バイオマスからのl−アミノ酸の製造方法
EP3061828B1 (en) 2013-10-23 2024-08-28 Ajinomoto Co., Inc. Method for producing target substance
EP3101130B1 (en) 2014-01-31 2020-05-06 Ajinomoto Co., Inc. Mutant glutamate-cysteine ligase and method for manufacturing gamma-glutamyl-valyl-glycine
JP2017216881A (ja) 2014-12-26 2017-12-14 味の素株式会社 ジカルボン酸の製造方法
TW202248422A (zh) 2015-05-19 2022-12-16 英商三菱化學英國有限公司 生物生產甲基丙烯酸之方法
WO2017073701A2 (en) 2015-10-27 2017-05-04 Ajinomoto Co., Inc. Method for producing aldehyde
JP6623690B2 (ja) 2015-10-30 2019-12-25 味の素株式会社 グルタミン酸系l−アミノ酸の製造法
CN108463555A (zh) 2016-01-12 2018-08-28 味之素株式会社 生产苯甲醛的方法
KR102337932B1 (ko) 2016-10-21 2021-12-13 아지노모토 가부시키가이샤 단백질의 분비 생산법
AU2017347016B2 (en) 2016-10-21 2021-09-23 Ajinomoto Co., Inc. Secretory production method for protein
WO2018079687A1 (en) 2016-10-26 2018-05-03 Ajinomoto Co., Inc. Method for producing objective substance
WO2018079683A1 (en) 2016-10-26 2018-05-03 Ajinomoto Co., Inc. Method for producing objective substance
CN109890972B (zh) 2016-10-26 2022-11-08 味之素株式会社 生产目标物质的方法
WO2018079684A1 (en) 2016-10-26 2018-05-03 Ajinomoto Co., Inc. Method for producing objective substance
CN109963947B (zh) 2016-10-26 2023-07-04 味之素株式会社 用于生产l-甲硫氨酸或需要s-腺苷甲硫氨酸进行合成的代谢物的方法
EP3532610B1 (en) 2016-10-27 2023-09-27 Ajinomoto Co., Inc. Method for producing aldehyde
GB201619827D0 (en) 2016-11-23 2017-01-04 Lucite Int Uk Ltd Process for the production of methyl methacrylate
EP3601583A1 (en) 2017-03-28 2020-02-05 Ajinomoto Co., Inc. Method for producing rna
JP7066977B2 (ja) 2017-04-03 2022-05-16 味の素株式会社 L-アミノ酸の製造法
US11155845B2 (en) 2017-04-13 2021-10-26 Kyowa Hakko Bio Co., Ltd. Method for producing theanine
US10858676B2 (en) 2017-05-22 2020-12-08 Ajinomoto Co., Inc. Method for producing objective substance
KR102801726B1 (ko) 2017-09-25 2025-05-07 아지노모토 가부시키가이샤 단백질의 제조법 및 2당의 제조법
WO2019078095A1 (en) 2017-10-16 2019-04-25 Ajinomoto Co., Inc. PROCESS FOR PRODUCTION OF PROTEIN HAVING ALPHA-HYDROXYLANTIC PEPTIDYLGLYCIN MONOOXYGENASE ACTIVITY
US11680279B2 (en) 2017-11-29 2023-06-20 Ajinomoto Co., Inc. Method for producing objective substance
EP3732298A1 (en) 2017-12-26 2020-11-04 Ajinomoto Co., Inc. Method of producing glycine by fermentation
JP7367667B2 (ja) 2018-02-20 2023-10-24 味の素株式会社 Rnaサイレンシングを誘導する方法
JP7124338B2 (ja) 2018-02-27 2022-08-24 味の素株式会社 変異型グルタチオン合成酵素、及び、γ-グルタミルバリルグリシンの製造法
AU2019256065B2 (en) 2018-04-20 2024-11-28 Ajinomoto Co., Inc. Method for secretory production of protein
WO2020027251A1 (en) 2018-08-03 2020-02-06 Ajinomoto Co., Inc. Method for producing objective substance
JP7540943B2 (ja) 2018-08-10 2024-08-27 協和発酵バイオ株式会社 多価不飽和脂肪酸を生産する微生物及び多価不飽和脂肪酸の製造法
JPWO2020032261A1 (ja) 2018-08-10 2021-08-10 協和発酵バイオ株式会社 エイコサペンタエン酸を生産する微生物及びエイコサペンタエン酸の製造法
EP3872096A4 (en) 2018-10-25 2022-11-30 Ajinomoto Co., Inc. PROCESS FOR THE SECRETORY PRODUCTION OF A PROTEIN
WO2020203885A1 (ja) 2019-03-29 2020-10-08 味の素株式会社 アロラクトースの製造法
CN113811524A (zh) 2019-05-08 2021-12-17 味之素株式会社 香兰素的制造方法
WO2021085405A1 (ja) 2019-10-28 2021-05-06 味の素株式会社 ベンズアルデヒドの製造方法
US20240279696A1 (en) 2019-12-16 2024-08-22 Kyowa Hakko Bio Co., Ltd. Microorganism having modified lactose permease, and method for producing lactose-containing oligosaccharide
BR112022017430A2 (pt) 2020-03-04 2022-10-18 Ajinomoto Kk Método e composição para produzir um alimento, transglutaminase mutante, gene, vetor, e, microrganismo
CN115667517A (zh) 2020-06-25 2023-01-31 协和发酵生化株式会社 二肽的制造方法
EP4223129A4 (en) 2020-09-29 2025-02-19 Ajinomoto Co., Inc. MUTANT TRANSGLUTAMINASE
CN116583605A (zh) 2020-10-28 2023-08-11 味之素株式会社 L-氨基酸的制造方法
CN116806260A (zh) 2021-02-08 2023-09-26 协和发酵生化株式会社 具有1,3-岩藻糖基转移酶活性的蛋白和含有岩藻糖的糖质的制造方法
EP4296360A4 (en) 2021-02-19 2025-10-22 Plumino Prec Fermentation Japan Co Ltd MODIFIED PROTEIN HAVING ALPHA1, 2-FUCOSYLTRANSFERASE ACTIVITY AND METHOD FOR PRODUCING FUCOSE-CONTAINING CARBOHYDRATE
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US20250215472A1 (en) 2022-03-25 2025-07-03 Kirin Holdings Kabushiki Kaisha Protein having alpha-1,2-fucosyltransferase activity and method for producing lacto-n-fucopentaose i (lnfpi)
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US20250270607A1 (en) 2022-04-28 2025-08-28 Kyowa Hakko Bio Co., Ltd. Protein having nampt activity, and method for producing nmn
CN119301241A (zh) 2022-06-06 2025-01-10 协和发酵生化株式会社 提高了3'-唾液酸乳糖的生产率的微生物和3'-唾液酸乳糖的制造方法
JPWO2024005155A1 (OSRAM) 2022-06-30 2024-01-04
GB2621337A (en) 2022-08-08 2024-02-14 Mitsubishi Chemical Uk Ltd Process for the biological production of methacrylic acid and derivatives thereof
JPWO2024122636A1 (OSRAM) 2022-12-09 2024-06-13
CN121420067A (zh) 2023-06-29 2026-01-27 麒麟控股株式会社 4-(氨甲基)环己烷-1-甲酸的制造方法
EP4692312A1 (en) 2023-12-28 2026-02-11 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing l-glutamic acid
JP7716823B1 (ja) 2023-12-28 2025-08-01 味の素株式会社 L-グルタミン酸生産菌およびl-グルタミン酸の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3003925A (en) * 1956-05-17 1961-10-10 Kyowa Hakko Kogyo Kk Method of producing l-glutamic acid by fermentation
US3002889A (en) * 1960-06-20 1961-10-03 Kyowa Hakko Kogyo Kk Method of producing l-glutamic acid
US3220929A (en) * 1964-02-10 1965-11-30 Kyowa Hakko Kogyo Kk Microbiological production of amino acid by reductive amination
GB2052516B (en) * 1979-06-01 1983-06-29 Searle & Co Plasmid vectors production and use thereof
JPS57183799A (en) * 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid

Also Published As

Publication number Publication date
US4617267A (en) 1986-10-14
EP0058889A1 (en) 1982-09-01
DE3274561D1 (en) 1987-01-15
CA1196588A (en) 1985-11-12
EP0058889B1 (en) 1986-12-03
JPS57134500A (en) 1982-08-19
JPH0112477B2 (OSRAM) 1989-03-01

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