DE483797T1 - Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease und -Methylase Nco I. - Google Patents

Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease und -Methylase Nco I.

Info

Publication number
DE483797T1
DE483797T1 DE91118511T DE91118511T DE483797T1 DE 483797 T1 DE483797 T1 DE 483797T1 DE 91118511 T DE91118511 T DE 91118511T DE 91118511 T DE91118511 T DE 91118511T DE 483797 T1 DE483797 T1 DE 483797T1
Authority
DE
Germany
Prior art keywords
methylase
nco
cloning
production
restriction endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE91118511T
Other languages
English (en)
Inventor
Elizabeth Merrill Vancott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE483797T1 publication Critical patent/DE483797T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE91118511T 1990-11-02 1991-10-30 Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease und -Methylase Nco I. Pending DE483797T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/608,228 US5202248A (en) 1990-11-02 1990-11-02 Method for cloning and producing the nco i restriction endonuclease and methylase

Publications (1)

Publication Number Publication Date
DE483797T1 true DE483797T1 (de) 1993-11-25

Family

ID=24435593

Family Applications (2)

Application Number Title Priority Date Filing Date
DE91118511T Pending DE483797T1 (de) 1990-11-02 1991-10-30 Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease und -Methylase Nco I.
DE69113390T Expired - Lifetime DE69113390T2 (de) 1990-11-02 1991-10-30 Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease Nco I.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69113390T Expired - Lifetime DE69113390T2 (de) 1990-11-02 1991-10-30 Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease Nco I.

Country Status (4)

Country Link
US (1) US5202248A (de)
EP (1) EP0483797B1 (de)
JP (1) JP3217409B2 (de)
DE (2) DE483797T1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2777947B2 (ja) * 1992-02-21 1998-07-23 寳酒造株式会社 Ｎｓｐ ７５２４Ｖ制限・修飾系酵素遺伝子及び製造方法
US5292651A (en) * 1992-05-12 1994-03-08 New England Biolabs, Inc. Method for cloning and producing the NaeI restriction endonuclease and methylase
US5262318A (en) * 1992-08-20 1993-11-16 New England Biolabs, Inc. Isolated DNA encoding the SPHI restriction endonuclease and related methods for producing the same
US5371006A (en) * 1993-01-11 1994-12-06 New England Biolabs, Inc. Isolated DNA encoding the NotI restriction endonuclease and related methods for producing the same
AU774643B2 (en) 1999-03-02 2004-07-01 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
EP1250453B1 (de) 1999-12-10 2008-04-09 Invitrogen Corporation Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
WO2004009768A2 (en) * 2002-07-18 2004-01-29 Invitrogen Corporation Viral vectors containing recombination sites
WO2004013290A2 (en) * 2002-08-05 2004-02-12 Invitrogen Corporation Compositions and methods for molecular biology
US6841806B1 (en) * 2003-06-24 2005-01-11 The University Of Connecticut Heterojunction thyristor-based amplifier
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005086654A2 (en) * 2004-02-27 2005-09-22 The Trustees Of Princeton University Self-cleaving affinity tags and methods of use
EP2540823A1 (de) 2005-08-04 2013-01-02 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
EP2390319B1 (de) * 2007-07-12 2014-12-24 New England Biolabs, Inc. Restriktionsendonukleasen mit hoher Fidelität
CN112662647A (zh) * 2021-01-08 2021-04-16 上海咏科生物科技有限公司 重组NcoI限制性内切酶的制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
ATE113653T1 (de) * 1986-06-06 1994-11-15 New England Biolabs Inc Verfahren zur klonierung eines restriktionsmodifizierungssystems.
US4983522A (en) * 1987-12-17 1991-01-08 New England Biolabs, Inc. Method for producing the HinPI restriction endonuclease and methylase
US4987074A (en) * 1987-12-17 1991-01-22 New England Biolabs, Inc. Method for producing the HgiAI restriction endonuclease and methylase
US4983542A (en) * 1987-12-21 1991-01-08 New England Biolabs, Inc. Method for producing the XbaI restriction endonuclease and methylase
US5015581A (en) * 1989-03-15 1991-05-14 New England Biolabs, Inc. Method for producing the Hinc II restriction endonuclease and methylase

Also Published As

Publication number Publication date
DE69113390D1 (de) 1995-11-02
EP0483797B1 (de) 1995-09-27
JPH05192166A (ja) 1993-08-03
US5202248A (en) 1993-04-13
JP3217409B2 (ja) 2001-10-09
EP0483797A1 (de) 1992-05-06
DE69113390T2 (de) 1996-05-15

Similar Documents

Publication Publication Date Title
DE395367T1 (de) Verfahren zur Herstellung von Restriktionsendonuklease und -methylase XamI.
DE69328758D1 (de) Verfahren zur Herstellung von SOI-Bipolar- und MOS-Transistoren
DE69434536D1 (de) Verfahren zur Herstellung von halbleitenden Wafern
DE3789881T2 (de) Verfahren zur Herstellung von Photomasken und Photomaske.
DE483797T1 (de) Verfahren zur Klonierung und Herstellung von Restriktionsendonuklease und -Methylase Nco I.
DE69221283T2 (de) Verfahren zur Herstellung von Polysilanen
DE59304719D1 (de) Verfahren zur Herstellung von Polysilazanen
DE69219428D1 (de) Verfahren zur Herstellung von Mitteldestillaten
DE69428845T2 (de) Verfahren zur Herstellung von Sende-Empfängern
DE68917372D1 (de) Verfahren zur Herstellung von Nde I-Restriktionsendonuklease und Methylase.
DE69023789D1 (de) Verfahren zur Herstellung der Hinc-II-Restriktionsendonuklease und Methylase.
DE69327822T2 (de) Verfahren zur Klonierung und zur Herstellung der AatII-Restriktionsendonuklease und -methylase
DE321270T1 (de) Verfahren zur Herstellung von HhaI-Restriktionsendonuklease und -methylase.
DE69313297T2 (de) Benzofuranone, benzodifurantrione und verfahren zur herstellung von benzodifurandionen
DE437100T1 (de) Verfahren zur Klonierung und Herstellung der SfiI-Restriktionsendonuklease und Methylase.
DE477532T1 (de) Verfahren zur Herstellung von Restriktionsendonuklease und -methylase Nla III.
DE69023681D1 (de) Verfahren zur Herstellung von Restriktionsendonuklease und -Methylase-MwoI.
DE577254T1 (de) Verfahren zur Klonierung und Herstellung der Nael-Endonuklease und Methylase.
DE475195T1 (de) Verfahren zur Herstellung und Klonierung der Sac-II-Restruktionsendonuklease und Methylase.
DE69114599D1 (de) Verfahren zur Herstellung von Esterase.
DE69424592T2 (de) Verfahren zur Klonierung und zur Herstellung der Restriktionsendonuklease und Modifikationsmethylase BglII
DE69323443D1 (de) Verfahren zur Herstellung von Blockpolyoxyalkylenepolyethern
DE69018189D1 (de) Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I.
DE69212782T2 (de) Verfahren zur Herstellung von Polyaminen und Polyisocyanaten
DE69116193T2 (de) Enzymatisches Verfahren zur Herstellung von Desoxyketosen