DE69018189T2 - Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I. - Google Patents

Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I.

Info

Publication number
DE69018189T2
DE69018189T2 DE69018189T DE69018189T DE69018189T2 DE 69018189 T2 DE69018189 T2 DE 69018189T2 DE 69018189 T DE69018189 T DE 69018189T DE 69018189 T DE69018189 T DE 69018189T DE 69018189 T2 DE69018189 T2 DE 69018189T2
Authority
DE
Germany
Prior art keywords
methylase
hpa
production
restriction endonuclease
endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69018189T
Other languages
English (en)
Other versions
DE69018189D1 (de
Inventor
Jack Stanley Benner
Phyllis Rees
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE69018189D1 publication Critical patent/DE69018189D1/de
Application granted granted Critical
Publication of DE69018189T2 publication Critical patent/DE69018189T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
DE69018189T 1989-10-13 1990-10-12 Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I. Expired - Lifetime DE69018189T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/421,272 US5298404A (en) 1989-10-13 1989-10-13 Method for producing the Hpa I restriction endonuclease and methylase

Publications (2)

Publication Number Publication Date
DE69018189D1 DE69018189D1 (de) 1995-05-04
DE69018189T2 true DE69018189T2 (de) 1995-11-09

Family

ID=23669879

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69018189T Expired - Lifetime DE69018189T2 (de) 1989-10-13 1990-10-12 Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I.
DE90311190T Pending DE422951T1 (de) 1989-10-13 1990-10-12 Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE90311190T Pending DE422951T1 (de) 1989-10-13 1990-10-12 Verfahren zur Herstellung von Restriktionsendonuklease und Methylase Hpa I.

Country Status (4)

Country Link
US (1) US5298404A (de)
EP (1) EP0422951B1 (de)
JP (1) JP3003117B2 (de)
DE (2) DE69018189T2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731185A (en) * 1995-07-21 1998-03-24 New England Biolabs, Inc. Isolated DNA encoding the hphi restriction endonuclease and related methods for producing the same
EP2562252A1 (de) 2005-08-04 2013-02-27 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
DE3751923T2 (de) * 1986-06-06 1997-04-03 New England Biolabs Inc Verfahren zur Klonierung von Restriktions-Modifikationssystemen
US5030569A (en) * 1987-10-15 1991-07-09 New England Biolabs, Inc. Method for producing the AFL II restriction endonuclease and methylase
US4999294A (en) * 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the FokI restriction endonuclease and methylase
US4987074A (en) * 1987-12-17 1991-01-22 New England Biolabs, Inc. Method for producing the HgiAI restriction endonuclease and methylase
US4988620A (en) * 1987-12-17 1991-01-29 New England Biolabs, Inc. Method for producing the FnuDI restriction endonuclease and methylase
US4999293A (en) * 1987-12-17 1991-03-12 New England Biolabs, Inc. Method for producing the HhaI restriction endonuclease and methylase
US4983522A (en) * 1987-12-17 1991-01-08 New England Biolabs, Inc. Method for producing the HinPI restriction endonuclease and methylase
US4983542A (en) * 1987-12-21 1991-01-08 New England Biolabs, Inc. Method for producing the XbaI restriction endonuclease and methylase
JPH02216708A (ja) * 1988-04-11 1990-08-29 Fuji Photo Film Co Ltd 導電性皮膜
US4996151A (en) * 1988-05-19 1991-02-26 New England Biolabs, Inc. Method for producing the Eag I restriction endonuclease and methylase
US5015581A (en) * 1989-03-15 1991-05-14 New England Biolabs, Inc. Method for producing the Hinc II restriction endonuclease and methylase
US5002882A (en) * 1989-04-27 1991-03-26 New England Biolabs, Inc. Method for producing the XmaI restriction endonuclease and methylase

Also Published As

Publication number Publication date
JP3003117B2 (ja) 2000-01-24
DE69018189D1 (de) 1995-05-04
EP0422951B1 (de) 1995-03-29
JPH03224488A (ja) 1991-10-03
DE422951T1 (de) 1994-02-03
US5298404A (en) 1994-03-29
EP0422951A1 (de) 1991-04-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition