DE68917372D1 - Verfahren zur Herstellung von Nde I-Restriktionsendonuklease und Methylase. - Google Patents

Verfahren zur Herstellung von Nde I-Restriktionsendonuklease und Methylase.

Info

Publication number
DE68917372D1
DE68917372D1 DE68917372T DE68917372T DE68917372D1 DE 68917372 D1 DE68917372 D1 DE 68917372D1 DE 68917372 T DE68917372 T DE 68917372T DE 68917372 T DE68917372 T DE 68917372T DE 68917372 D1 DE68917372 D1 DE 68917372D1
Authority
DE
Germany
Prior art keywords
methylase
nde
preparation
restriction endonuclease
endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68917372T
Other languages
English (en)
Other versions
DE68917372T2 (de
Inventor
Jack Stanley Benner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of DE68917372D1 publication Critical patent/DE68917372D1/de
Application granted granted Critical
Publication of DE68917372T2 publication Critical patent/DE68917372T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE68917372T 1988-05-19 1989-05-19 Verfahren zur Herstellung von Nde I-Restriktionsendonuklease und Methylase. Expired - Lifetime DE68917372T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/196,028 US5139942A (en) 1988-05-19 1988-05-19 Method for producing the nde i restriction endonuclease and methylase

Publications (2)

Publication Number Publication Date
DE68917372D1 true DE68917372D1 (de) 1994-09-15
DE68917372T2 DE68917372T2 (de) 1995-03-09

Family

ID=22723846

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68917372T Expired - Lifetime DE68917372T2 (de) 1988-05-19 1989-05-19 Verfahren zur Herstellung von Nde I-Restriktionsendonuklease und Methylase.

Country Status (4)

Country Link
US (1) US5139942A (de)
EP (1) EP0343009B1 (de)
JP (1) JP3162059B2 (de)
DE (1) DE68917372T2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5288696A (en) * 1990-09-07 1994-02-22 New England Biolabs, Inc. Method for producing and cloning SacII restriction endonuclease and methylase
AU774643B2 (en) 1999-03-02 2004-07-01 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
EP1250453B1 (de) 1999-12-10 2008-04-09 Invitrogen Corporation Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP4210769B2 (ja) * 2001-11-22 2009-01-21 独立行政法人産業技術総合研究所 Ｇａｔｅｗａｙエントリークローンの作製方法
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005086654A2 (en) * 2004-02-27 2005-09-22 The Trustees Of Princeton University Self-cleaving affinity tags and methods of use
EP2540823A1 (de) 2005-08-04 2013-01-02 New England Biolabs, Inc. Neuartige Restriktionsendonukleasen, DNA-Kodierung dieser Endonukleasen und Verfahren zur Identifizierung neuer Endonukleasen mit der gleichen oder variierten Spezifizität
US10429869B2 (en) 2011-02-16 2019-10-01 Kortek Industries Pty Ltd Wireless power, light and automation control
WO2016030501A1 (en) 2014-08-28 2016-03-03 Centre National De La Recherche Scientifique - Cnrs - Synthetic alu-retrotransposon vectors for gene therapy
CN110438102A (zh) * 2019-09-03 2019-11-12 莫纳(武汉)生物科技有限公司 一种甲基化保护宿主菌及其构建方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN166864B (de) * 1985-03-01 1990-07-28 New England Biolabs Inc
ATE113653T1 (de) * 1986-06-06 1994-11-15 New England Biolabs Inc Verfahren zur klonierung eines restriktionsmodifizierungssystems.

Also Published As

Publication number Publication date
JPH0220285A (ja) 1990-01-23
US5139942A (en) 1992-08-18
EP0343009B1 (de) 1994-08-10
EP0343009A2 (de) 1989-11-23
JP3162059B2 (ja) 2001-04-25
EP0343009A3 (en) 1990-03-21
DE68917372T2 (de) 1995-03-09

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