DD276886A5 - Expressin und Sekretion heterologer Proteine durch transformierte Spezies von Yarrowia Lipolytica - Google Patents

Expressin und Sekretion heterologer Proteine durch transformierte Spezies von Yarrowia Lipolytica Download PDF

Info

Publication number
DD276886A5
DD276886A5 DD29535886A DD29535886A DD276886A5 DD 276886 A5 DD276886 A5 DD 276886A5 DD 29535886 A DD29535886 A DD 29535886A DD 29535886 A DD29535886 A DD 29535886A DD 276886 A5 DD276886 A5 DD 276886A5
Authority
DD
German Democratic Republic
Prior art keywords
lipolytica
gene
dna
transformed
xpr2
Prior art date
Application number
DD29535886A
Other languages
German (de)
English (en)
Inventor
Lance Steven Davidow
John Robert Dezeeuw
Arthur Ernest Franke
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of DD276886A5 publication Critical patent/DD276886A5/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/472—Complement proteins, e.g. anaphylatoxin, C3a, C5a
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Magnetic Ceramics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Sludge (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Paper (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)
  • Fodder In General (AREA)
  • Noodles (AREA)
DD29535886A 1985-10-18 1986-10-17 Expressin und Sekretion heterologer Proteine durch transformierte Spezies von Yarrowia Lipolytica DD276886A5 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78920685A 1985-10-18 1985-10-18
US06/841,121 US4937189A (en) 1985-10-18 1986-03-18 Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants

Publications (1)

Publication Number Publication Date
DD276886A5 true DD276886A5 (de) 1990-03-14

Family

ID=27120892

Family Applications (7)

Application Number Title Priority Date Filing Date
DD29535886A DD276886A5 (de) 1985-10-18 1986-10-17 Expressin und Sekretion heterologer Proteine durch transformierte Spezies von Yarrowia Lipolytica
DD31347586A DD267998A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von transformierter Yarrowia Lipolytica, die keinealkalische Protease produziert
DD31347386A DD267996A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von Plasmid Pxx-22
DD31347186A DD269396A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von Plasmid Pxx-33
DD31347286A DD269397A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von transformierten Yarrowia Lipolytica
DD31347086A DD267995A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von Plasmid Pxx-11
DD31347486A DD267997A5 (de) 1985-10-18 1986-10-17 Verfahren zur Auffindung von transformierten Yarrowia Lipolytica-Spezies

Family Applications After (6)

Application Number Title Priority Date Filing Date
DD31347586A DD267998A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von transformierter Yarrowia Lipolytica, die keinealkalische Protease produziert
DD31347386A DD267996A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von Plasmid Pxx-22
DD31347186A DD269396A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von Plasmid Pxx-33
DD31347286A DD269397A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von transformierten Yarrowia Lipolytica
DD31347086A DD267995A5 (de) 1985-10-18 1986-10-17 Verfahren zum Herstellen von Plasmid Pxx-11
DD31347486A DD267997A5 (de) 1985-10-18 1986-10-17 Verfahren zur Auffindung von transformierten Yarrowia Lipolytica-Spezies

Country Status (24)

Country Link
US (1) US4937189A (no)
EP (1) EP0220864B1 (no)
JP (1) JP3096297B2 (no)
KR (1) KR900008950B1 (no)
CN (3) CN1034428C (no)
AT (1) ATE86302T1 (no)
AU (1) AU586041B2 (no)
CA (1) CA1340837C (no)
DD (7) DD276886A5 (no)
DE (1) DE3687878T2 (no)
DK (1) DK496886A (no)
EG (1) EG18142A (no)
ES (1) ES2038966T3 (no)
FI (1) FI90352C (no)
HU (1) HU209150B (no)
IE (1) IE59774B1 (no)
IL (7) IL80297A (no)
NO (2) NO177318C (no)
NZ (1) NZ217985A (no)
PH (3) PH27026A (no)
PL (2) PL155850B1 (no)
PT (1) PT83562B (no)
RU (2) RU2157845C2 (no)
YU (2) YU46412B (no)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
EP0382332A3 (en) * 1989-01-06 1990-11-14 Collaborative Research Inc. Improved supersecreting mutants of saccharomyces cerevisiae
JPH0669365B2 (ja) * 1989-05-22 1994-09-07 株式会社ミドリ十字 アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法
EP0402226A1 (en) * 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
EP0716704A1 (en) * 1993-09-02 1996-06-19 Pfizer Inc. MULTIPLE INTEGRATIVE VECTORS AND $i(YARROWIA LIPOLYTICA) TRANSFORMANTS
US5807824A (en) * 1993-12-06 1998-09-15 Ciba-Geigy Corporation C5A receptor antagonists having substantially no agonist activity
US5837499A (en) * 1993-12-06 1998-11-17 Ciba-Geigy Corporation DNA encoding C5A receptor antagonists having substantially no agonist activity and methods of expressing same
DE19513152A1 (de) 1995-04-07 1996-10-10 Bundesrep Deutschland Verwendung eines "Immundefizienzvirus-supprimierenden Lymphokins (ISL)" zur Hemmung der Virusvermehrung, insbesondere von Retroviren
EP0832223A1 (en) * 1995-06-05 1998-04-01 Novartis AG C5a RECEPTOR ANTAGONISTS HAVING SUBSTANTIALLY NO AGONIST ACTIVITY AND METHODS FOR PREPARATION
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
FR2782733B1 (fr) * 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
US7459546B2 (en) 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7202356B2 (en) * 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
US7264949B2 (en) * 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US20060094102A1 (en) * 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
WO2006102342A2 (en) 2005-03-18 2006-09-28 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
WO2010004141A2 (fr) * 2008-07-11 2010-01-14 Institut National De La Recherche Agronomique (Inra) Nouvelles souches de levure mutantes capables d'accumuler une grande quantité de lipides
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
BR112013016769A2 (pt) * 2010-12-30 2016-10-11 Du Pont yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse
WO2012135773A1 (en) 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
EP2694657A1 (en) 2011-04-05 2014-02-12 E. I. Du Pont de Nemours and Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
GB201106188D0 (en) 2011-04-12 2011-05-25 Csir Production of extracellular polypeptides
FR3002774A1 (fr) 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel
WO2015116781A2 (en) 2014-01-31 2015-08-06 Dsm Ip Assets B.V. Promoters suitable for heterologous gene expression in yeast
EP3155094A1 (en) 2014-06-11 2017-04-19 Institut National De La Recherche Agronomique (INRA) Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
WO2017100158A1 (en) 2015-12-11 2017-06-15 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
EP3390631B1 (en) 2015-12-18 2020-04-08 Danisco US Inc. Methods and compositions for t-rna based guide rna expression
EP3455362A1 (en) 2016-05-10 2019-03-20 Institut National De La Recherche Agronomique Mutant yeast strains with enhanced production of erythritol or erythrulose
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
IN163393B (no) * 1983-10-06 1988-09-17 Pfizer
US4628033A (en) * 1983-10-06 1986-12-09 Pfizer Inc. Novel host strain for transformation of Yarrowia lipolytica
DE3572688D1 (en) * 1984-06-26 1989-10-05 Agronomique Inst Nat Rech Transformation vector for yeast yarrowia lipolytica, transformation process and transformed yeast

Also Published As

Publication number Publication date
PL155850B1 (en) 1992-01-31
AU6416186A (en) 1987-05-14
KR870004138A (ko) 1987-05-07
YU177386A (en) 1989-04-30
CN1138632A (zh) 1996-12-25
PH27026A (en) 1993-02-01
NO177318B (no) 1995-05-15
IL99894A0 (en) 1992-08-18
AU586041B2 (en) 1989-06-29
NO864148L (no) 1987-04-21
IE59774B1 (en) 1994-04-06
ES2038966T3 (es) 1993-08-16
PH27171A (en) 1993-04-02
IL99893A0 (en) 1992-08-18
CA1340837C (en) 1999-12-07
CN1034428C (zh) 1997-04-02
IL80297A0 (en) 1987-01-30
DK496886A (da) 1987-06-11
NO923616D0 (no) 1992-09-17
IL99893A (en) 1993-02-21
NO178793C (no) 1996-06-05
FI864206A0 (fi) 1986-10-17
EG18142A (en) 1992-08-30
PT83562B (pt) 1989-05-31
CN1141339A (zh) 1997-01-29
DD269396A5 (de) 1989-06-28
DD267996A5 (de) 1989-05-17
IL99894A (en) 1993-02-21
EP0220864B1 (en) 1993-03-03
RU2069694C1 (ru) 1996-11-27
PL154427B1 (en) 1991-08-30
DD267995A5 (de) 1989-05-17
IL80297A (en) 1993-02-21
DE3687878D1 (de) 1993-04-08
HUT43632A (en) 1987-11-30
NO864148D0 (no) 1986-10-17
HU209150B (en) 1994-03-28
CN1069346C (zh) 2001-08-08
NO178793B (no) 1996-02-26
ATE86302T1 (de) 1993-03-15
DD267998A5 (de) 1989-05-17
FI864206A (fi) 1987-04-19
IE862754L (en) 1987-04-18
FI90352C (fi) 1994-01-25
NZ217985A (en) 1991-06-25
DK496886D0 (da) 1986-10-17
PT83562A (en) 1986-11-01
PL261912A1 (en) 1987-10-19
CN86106522A (zh) 1987-09-16
YU46571B (sh) 1993-11-16
FI90352B (fi) 1993-10-15
IL99892A (en) 1993-02-21
KR900008950B1 (ko) 1990-12-15
YU124588A (en) 1989-08-31
US4937189A (en) 1990-06-26
PH27198A (en) 1993-04-16
IL99892A0 (en) 1992-08-18
YU46412B (sh) 1993-10-20
DE3687878T2 (de) 1993-07-15
DD269397A5 (de) 1989-06-28
JP3096297B2 (ja) 2000-10-10
JPS63164889A (ja) 1988-07-08
RU2157845C2 (ru) 2000-10-20
EP0220864A1 (en) 1987-05-06
NO923616L (no) 1987-04-21
DD267997A5 (de) 1989-05-17
NO177318C (no) 1995-08-23

Similar Documents

Publication Publication Date Title
DD276886A5 (de) Expressin und Sekretion heterologer Proteine durch transformierte Spezies von Yarrowia Lipolytica
DE68920882T3 (de) Aspartat-proteinase-defiziente filamentöse fungi.
DE3308215C2 (de) Expression, Processing und Sekretion von heterologem Protein durch Hefe
DE69532603T2 (de) Hefestämme und modifizierte albumine
DE4226971C2 (de) Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
DE69532283T2 (de) EIN PILZ, IN DEM DAS areA-GEN VERÄNDERT IST, UND EIN areA-GEN AUS ASPERGILLUS ORYZAE
EP0327797B1 (de) Verfahren zur Herstellung von Proteinen oder proteinhaltigen Genprodukten
DD218118A5 (de) Verfahren zur herstellung eines hefe- oder nichthefepolypeptids
DD255544A5 (de) Verfahren zur ortsselektiven Genommodifikation von Hefen der Gattung Pichia
DD284047A5 (de) Verfahren zur herstellung einer dna-sequenz, die fuer einen alkohol-oxidose-ii-regulationsbereich einer methylotrophen hefe kodiert
DE69333249T2 (de) Neue Schimmel-Protease
EP0324712B1 (de) Hirudin-Derivat
EP0300425B1 (de) Verfahren zur Herstellung von Proteinen in löslicher Form
WO1992000379A1 (de) Neue promotorregion
EP0323838B1 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten
EP0569806A2 (de) DNA-Verbindungen und rekombinante, die Ribloflavinsynthetaseaktivität von S. cerevisiae codierende DNA-Expressionsvektoren
DE3804890C2 (de) Verfahren zur Herstellung von Proteinen oder proteinhaltigen Genprodukten
DE3900626A1 (de) Hirudin-derivat
DE3444495A1 (de) Herstellung eines multi-kopien-hefe-vektors
DD283839A5 (de) Verfahren zur herstellung eines hefestammes ohne carboxyteptidase-ysc alpha-aktivitaet
DD257646A5 (de) Verfahren zur Herstellung von Streptokinase
DD264455A1 (de) Verfahren zur synthese von chymosin
DD284693A5 (de) Verfahren zur herstellung rekombinanter dna-molekuele mit einer codierung fuer pektonlyaseexpressionssysteme
DD243941A5 (de) Verfahren zur Herstellung eines multi-Kopien-Hefe-Vektors
DD253641A1 (de) Verfahren zur synthese von chymosin

Legal Events

Date Code Title Description
NPV Change in the person, the name or the address of the representative (addendum to changes before extension act)
ENJ Ceased due to non-payment of renewal fee