DE3687878T2 - Expression und sekretion von heterologen proteinen durch transformanten von yarrowia lipolytica. - Google Patents

Expression und sekretion von heterologen proteinen durch transformanten von yarrowia lipolytica.

Info

Publication number
DE3687878T2
DE3687878T2 DE8686307839T DE3687878T DE3687878T2 DE 3687878 T2 DE3687878 T2 DE 3687878T2 DE 8686307839 T DE8686307839 T DE 8686307839T DE 3687878 T DE3687878 T DE 3687878T DE 3687878 T2 DE3687878 T2 DE 3687878T2
Authority
DE
Germany
Prior art keywords
lipolytica
heterologous
xpr2
expression
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8686307839T
Other languages
English (en)
Other versions
DE3687878D1 (de
Inventor
Lance Steven Davidow
Arthur Ernest Franke
John Robert Dezeeuw
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc filed Critical Pfizer Inc
Publication of DE3687878D1 publication Critical patent/DE3687878D1/de
Application granted granted Critical
Publication of DE3687878T2 publication Critical patent/DE3687878T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/472—Complement proteins, e.g. anaphylatoxin, C3a, C5a
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Sludge (AREA)
  • Magnetic Ceramics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Paper (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Fodder In General (AREA)
  • Noodles (AREA)
  • Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
DE8686307839T 1985-10-18 1986-10-10 Expression und sekretion von heterologen proteinen durch transformanten von yarrowia lipolytica. Expired - Lifetime DE3687878T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78920685A 1985-10-18 1985-10-18
US06/841,121 US4937189A (en) 1985-10-18 1986-03-18 Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants

Publications (2)

Publication Number Publication Date
DE3687878D1 DE3687878D1 (de) 1993-04-08
DE3687878T2 true DE3687878T2 (de) 1993-07-15

Family

ID=27120892

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686307839T Expired - Lifetime DE3687878T2 (de) 1985-10-18 1986-10-10 Expression und sekretion von heterologen proteinen durch transformanten von yarrowia lipolytica.

Country Status (24)

Country Link
US (1) US4937189A (de)
EP (1) EP0220864B1 (de)
JP (1) JP3096297B2 (de)
KR (1) KR900008950B1 (de)
CN (3) CN1034428C (de)
AT (1) ATE86302T1 (de)
AU (1) AU586041B2 (de)
CA (1) CA1340837C (de)
DD (7) DD267996A5 (de)
DE (1) DE3687878T2 (de)
DK (1) DK496886A (de)
EG (1) EG18142A (de)
ES (1) ES2038966T3 (de)
FI (1) FI90352C (de)
HU (1) HU209150B (de)
IE (1) IE59774B1 (de)
IL (7) IL99892A (de)
NO (2) NO177318C (de)
NZ (1) NZ217985A (de)
PH (3) PH27026A (de)
PL (2) PL154427B1 (de)
PT (1) PT83562B (de)
RU (2) RU2069694C1 (de)
YU (2) YU46412B (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
EP0382332A3 (de) * 1989-01-06 1990-11-14 Collaborative Research Inc. Mutanten von Saccharomyces cerevisiae mit stark vermehrter Sekretion
JPH0669365B2 (ja) * 1989-05-22 1994-09-07 株式会社ミドリ十字 アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法
EP0402226A1 (de) * 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformationsvektoren für Hefe Yarrowia
WO1995006739A1 (en) * 1993-09-02 1995-03-09 Pfizer Inc. Multiple integrative vectors and yarrowia lipolytica transformants
US5837499A (en) * 1993-12-06 1998-11-17 Ciba-Geigy Corporation DNA encoding C5A receptor antagonists having substantially no agonist activity and methods of expressing same
US5807824A (en) * 1993-12-06 1998-09-15 Ciba-Geigy Corporation C5A receptor antagonists having substantially no agonist activity
DE19513152A1 (de) * 1995-04-07 1996-10-10 Bundesrep Deutschland Verwendung eines "Immundefizienzvirus-supprimierenden Lymphokins (ISL)" zur Hemmung der Virusvermehrung, insbesondere von Retroviren
JPH11507215A (ja) * 1995-06-05 1999-06-29 ノバルティス・アクチエンゲゼルシャフト アゴニスト活性を実質的にもたないＣ５ａ受容体アンタゴニストおよび製造方法
EP0747484A1 (de) * 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Aufwährts-aktivierungssequenzen und rekombinante Promotorsequenzen die in Yarrowia funktionell sind, und Vektoren die sie enthalten
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
FR2782733B1 (fr) 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
US7459546B2 (en) 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
WO2005049805A2 (en) * 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
US7264949B2 (en) * 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US20060094102A1 (en) * 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7851199B2 (en) * 2005-03-18 2010-12-14 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
US8597931B2 (en) * 2008-07-11 2013-12-03 Institut National De La Recherche Agronomique (Inra) Mutant yeast strains capable of accumulating a large quantity of lipids
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
US8735137B2 (en) 2010-12-30 2014-05-27 E I Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
WO2012135773A1 (en) 2011-03-31 2012-10-04 E. I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
GB201106188D0 (en) 2011-04-12 2011-05-25 Csir Production of extracellular polypeptides
FR3002774A1 (fr) 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel
US10611807B2 (en) 2014-01-31 2020-04-07 Dsm Ip Assets B.V. Promoters suitable for heterologous gene expression in yeast
EP3155094A1 (de) 2014-06-11 2017-04-19 Institut National De La Recherche Agronomique (INRA) Verbesserte lipidanlagerung in yarrowia-lipolytica-stämmen durch überexpression von hexokinase sowie neue stämme daraus
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
EP3387134B1 (de) 2015-12-11 2020-10-14 Danisco US Inc. Verfahren und zusammensetzungen für verbesserte nukleasevermittelte genommodifizierung und verringerte off-target-nebenwirkungen
EP3708665A1 (de) 2015-12-18 2020-09-16 Danisco US Inc. Verfahren und zusammensetzungen zur t-rna-basierten guide-rna-expression
US20190136278A1 (en) 2016-05-10 2019-05-09 Institut National De La Recherche Agronomique Mutant yeast strains with enhanced production of erythritol or erythrulose
EP3348647A1 (de) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutanter hefestamm mit fähigkeit zur produktion von fettsäuren mittlerer länge

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4628033A (en) * 1983-10-06 1986-12-09 Pfizer Inc. Novel host strain for transformation of Yarrowia lipolytica
IN163393B (de) * 1983-10-06 1988-09-17 Pfizer
EP0166659B1 (de) * 1984-06-26 1989-08-30 Institut National De La Recherche Agronomique (Inra) Transformationsvektor für Hefe Yarrowia lipolytica, Transformationsverfahren und transformierte Hefe

Also Published As

Publication number Publication date
IL99894A (en) 1993-02-21
IE862754L (en) 1987-04-18
JPS63164889A (ja) 1988-07-08
KR870004138A (ko) 1987-05-07
PL155850B1 (en) 1992-01-31
PL261912A1 (en) 1987-10-19
KR900008950B1 (ko) 1990-12-15
RU2157845C2 (ru) 2000-10-20
CN1141339A (zh) 1997-01-29
IL99892A (en) 1993-02-21
PH27198A (en) 1993-04-16
EG18142A (en) 1992-08-30
YU177386A (en) 1989-04-30
FI90352C (fi) 1994-01-25
NO923616D0 (no) 1992-09-17
DD269397A5 (de) 1989-06-28
DD267997A5 (de) 1989-05-17
PH27171A (en) 1993-04-02
RU2069694C1 (ru) 1996-11-27
YU46412B (sh) 1993-10-20
NO178793B (no) 1996-02-26
NO923616L (no) 1987-04-21
FI864206A (fi) 1987-04-19
PT83562B (pt) 1989-05-31
IL80297A (en) 1993-02-21
CN86106522A (zh) 1987-09-16
DD267995A5 (de) 1989-05-17
ATE86302T1 (de) 1993-03-15
JP3096297B2 (ja) 2000-10-10
NO864148D0 (no) 1986-10-17
US4937189A (en) 1990-06-26
IL99892A0 (en) 1992-08-18
IL80297A0 (en) 1987-01-30
DE3687878D1 (de) 1993-04-08
DD267996A5 (de) 1989-05-17
EP0220864A1 (de) 1987-05-06
IL99893A0 (en) 1992-08-18
FI90352B (fi) 1993-10-15
IL99893A (en) 1993-02-21
IL99894A0 (en) 1992-08-18
EP0220864B1 (de) 1993-03-03
YU124588A (en) 1989-08-31
PL154427B1 (en) 1991-08-30
DD269396A5 (de) 1989-06-28
AU586041B2 (en) 1989-06-29
DD276886A5 (de) 1990-03-14
HU209150B (en) 1994-03-28
NO178793C (no) 1996-06-05
CA1340837C (en) 1999-12-07
PH27026A (en) 1993-02-01
DD267998A5 (de) 1989-05-17
AU6416186A (en) 1987-05-14
IE59774B1 (en) 1994-04-06
DK496886D0 (da) 1986-10-17
NO177318B (no) 1995-05-15
NO864148L (no) 1987-04-21
HUT43632A (en) 1987-11-30
PT83562A (en) 1986-11-01
NZ217985A (en) 1991-06-25
DK496886A (da) 1987-06-11
FI864206A0 (fi) 1986-10-17
CN1138632A (zh) 1996-12-25
CN1069346C (zh) 2001-08-08
NO177318C (no) 1995-08-23
CN1034428C (zh) 1997-04-02
YU46571B (sh) 1993-11-16
ES2038966T3 (es) 1993-08-16

Similar Documents

Publication Publication Date Title
DE3687878D1 (de) Expression und sekretion von heterologen proteinen durch transformanten von yarrowia lipolytica.
ATE46536T1 (de) Verfahren zur herstellung von reifen proteinen in wirtzellen von wirbeltieren.
IL80022A (en) Cloned human basic fibroblast growth factor, methods for its preparation, compositions containing it, dna sequences encoding therefor and vectors and host cells containing said dna
ES474852A1 (es) Un metodo para la expresion,en un huesped bacteriano trans- formado de un polipeptido heterologo con respecto al huespeden su estado no transformado
EP0196864A3 (de) Behandlung und Sekretion von rekombinanten Proteinen mittels alkalischer Phosphatase, dazu verwendbare DNA-Sequenzen und mittels dieser Sequenzen transformierte Zellen
FR2724183B1 (fr) Vecteurs de criblage et/ou d'expression fonctionnels chez les mycobacteries - utilisation pour l'identification et l'expression de polypeptides exportes
ES8800721A1 (es) Un metodo para producir un granulo intracelular de la hormona bovina del crecimiento, altamente homogeneo.
EP0266057A3 (de) Eine eukaryotische sekretorische Signalsequenz enthaltendes Plasmid und Verfahren zur Sekretion der kodierten Proteine
KR910000180A (ko) 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법
RU92004585A (ru) Промотор, днк-последовательность, вектор, клетки, способ экспрессии днк
LTIP237A (en) Po438, a new calcium-regulated promotor
ES8705040A1 (es) Un procedimiento para la preparacion de un vector de expre- sion de una recombinante

Legal Events

Date Code Title Description
8364 No opposition during term of opposition