KR910000180A - 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 - Google Patents

트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 Download PDF

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KR910000180A
KR910000180A KR1019900008461A KR900008461A KR910000180A KR 910000180 A KR910000180 A KR 910000180A KR 1019900008461 A KR1019900008461 A KR 1019900008461A KR 900008461 A KR900008461 A KR 900008461A KR 910000180 A KR910000180 A KR 910000180A
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inhibitory activity
polypeptide
plasmid
thrombin inhibitory
secretory
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KR930003913B1 (ko
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요시오 후루따니
마사루 혼조오
아끼라 나까야마
고오이찌 가와무라
가즈노리 안도오
미찌꼬 호리
게이꼬 후까자와
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사와무라 하루오
미쓰이도오 아쓰가가꾸 가부시끼가이샤
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C12N15/09—Recombinant DNA-technology
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
    • F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
    • F02B1/00—Engines characterised by fuel-air mixture compression
    • F02B1/02—Engines characterised by fuel-air mixture compression with positive ignition
    • F02B1/04—Engines characterised by fuel-air mixture compression with positive ignition with fuel-air mixture admission into cylinder

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Abstract

내용 없음

Description

트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 이종 단백질 발현 분비 백터, pNPA225를 제조하는 방법을 나타낸다.
제2도는 HV1형 히루딘 분비플라스마, pHph208를 제조하는 방법을 나타낸다.
제3도는 히루딘을 코딩하는 DNA서열을 함유하는 하이브리드 플라스미드 p3009를 제조하는 방법을 나타낸다.
제4도는 히루딘을 분비하는 플라스미드, pNPH141를 제조하는 방법을 나타낸다.

Claims (14)

  1. 하기 아미노산 서열을 갖는, 트롬빈 억제 활성을 갖는 폴리펩티드의 전구체,
  2. 하기 아미노산 서열을 갖는, 트롬빈 억제 활성을 갖는 폴리펩티드,
  3. 제1항에 기재된 트롬빈 억제 활성을 갖는 폴리펩티드의 전구체를 코딩하는 하기의 DNA 서열.
  4. 제2항에 기재된 트롬빈 억제 활성을 갖는 폴리펩티드의 전구체를 코딩하는 하기의 DNA 서열.
  5. (a)프로모터 영역 및 리보소옴 결합 부위를 코팅하는 영역을 함유하는 DNA서열, (b) DNA서열(a)의 3'말단에 결합 가능한, 제1항에 기재된 전구체를 코딩하는 DNA서열, 및 (c)숙주에서 복제가능한 벡터DNA를 함유하는 분비 플라스미드.
  6. 제5항에 있어서, DNA서열(a)내의 여역이 바실루스 아밀로리퀴파시엔스(Bacillus amyloliquifaciens)의 중성 프로테아제 유전자의 것이 분비 플라스미드.
  7. 제6항에 있어서, (a)와 (b)의 결합 DNA 서열이 하기와 같은 분비 플라스미드.
  8. 제5항 내지 제7항중 어느 한 항에 있어서, 벡터 DNA가 바실루스속의 세균에서 복제 가능한 플라스미드인 분비 플라스미드.
  9. 제8항에 있어서, 프라스미드가 pUB110인 분비 플라스미드.
  10. 제9항에 있어서, 분비 플라스미드가 pNPH141인 분비 플라스미드.
  11. 제5항 내지 제10항에 기재된 분비 플라스미드 중의 하나를 사용하여 형질 전환된 형질전환체.
  12. 제11항에 있어서, 형질전환된 미생물이 바실루스 서브틸리스 (Bacillus subtilis)인 형질전환체.
  13. 제12항에 있어서, 바실루스 서브틸리스가 바실루스 서브틸리스 FERM BP-2403인 형질전환체.
  14. 제12항 또는 제13항의 형질 전환체를 배양하고 배양 상충액으로부터 트롬빈 억제 활성을 갖는 폴리펩티드를 회수하는 단계를 특징으로 하는, 트롬빈 억제 활성을 갖는 폴리펩티드의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900008461A 1989-06-09 1990-06-09 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법 KR930003913B1 (ko)

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JP?1-145489 1989-06-09
JP1145489A JP2859297B2 (ja) 1989-06-09 1989-06-09 トロンビン阻害活性を有するポリペプチドおよびその製造法
JP1-145489 1989-06-09

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KR910000180A true KR910000180A (ko) 1991-01-29
KR930003913B1 KR930003913B1 (ko) 1993-05-15

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US (1) US5166318A (ko)
EP (1) EP0402159B1 (ko)
JP (1) JP2859297B2 (ko)
KR (1) KR930003913B1 (ko)
CA (1) CA2018211C (ko)
DE (1) DE69013403T2 (ko)

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US5624822A (en) * 1989-12-22 1997-04-29 Basf Aktiengesellschaft Hirudin fusion proteins and preparation of hirudin
EP0624642B1 (en) * 1993-05-12 1999-01-20 Indian Council For Medical Research Novel thrombolytic agent, process for preparing same and its use for the preparation of a thrombi dissolving medicament
CN115572329B (zh) * 2021-06-21 2024-02-06 王大勇 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

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EP0151760A1 (en) * 1984-01-06 1985-08-21 Takeda Chemical Industries, Ltd. Novel DNA and use thereof
GB2171703B (en) * 1985-01-18 1989-11-15 Agency Ind Science Techn Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
FR2611723B1 (fr) * 1987-02-27 1989-09-08 Transgene Sa Vecteur d'expression d'adn recombinant codant pour l'hirudine, cultures de cellules de mammiferes infectees ou transformees, procede de preparation de l'hirudine et proteine obtenue

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JP2859297B2 (ja) 1999-02-17
KR930003913B1 (ko) 1993-05-15
AU620721B2 (en) 1992-02-20
CA2018211C (en) 1996-02-20
AU5697090A (en) 1990-12-13
US5166318A (en) 1992-11-24
JPH0310694A (ja) 1991-01-18
EP0402159B1 (en) 1994-10-19
DE69013403D1 (de) 1994-11-24
DE69013403T2 (de) 1995-02-23
CA2018211A1 (en) 1990-12-09
EP0402159A1 (en) 1990-12-12

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