ES8605040A1 - Procedimiento para preparar un polipeptido con actividad de hirudina - Google Patents

Procedimiento para preparar un polipeptido con actividad de hirudina

Info

Publication number
ES8605040A1
ES8605040A1 ES545994A ES545994A ES8605040A1 ES 8605040 A1 ES8605040 A1 ES 8605040A1 ES 545994 A ES545994 A ES 545994A ES 545994 A ES545994 A ES 545994A ES 8605040 A1 ES8605040 A1 ES 8605040A1
Authority
ES
Spain
Prior art keywords
gly
glu
cys
ile
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES545994A
Other languages
English (en)
Other versions
ES545994A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES8605040A1 publication Critical patent/ES8605040A1/es
Publication of ES545994A0 publication Critical patent/ES545994A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

PROCEDIMIENTO PARA PREPARAR UN POLIPEPTIDO CON ACTIVIDAD DE HIRUDINA. CONSISTE EN INCORPORAR EN UN PLASMIDO DE EXPRESION UN GEN QUE CODIFICA UN POLIPEPTIDO DE FORMULA (I), NO PRESENTANDOSE OTROS DE LOS AMINOACIDOS O PUDIENDO SER REEMPLAZADO O REEMPLAZADOS POR UNO O VARIOS OTRO(S) AMINOACIDO(S) GENETICAMENTE CODIFICABLE(S), DONDE M ES DE 0 A 50; N ES DE 0 A 100 Y X REPRESENTA RADICALES IGUALES O DIFERENTES DE AMINOACIDOS GENETICAMENTE CODIFICABLES; A ES ILE O UN ENLACE DIRECTO; B ES ILE, THR O UN ENLACE DIRECTO; C ES THR, VAL, ILE, LEU O PHE; D ES THR O UN ENLACE DIRECTO; E ES THR O ILE; F ES GLY O UN ENLACE DIRECTO; G ES GLU O UN ENLACE DIRECTO; H ES GLU O PRO; Y Z REPRESENTA RADICALES IGUALES O DIFERENTES DE AMINOACIDOS GENETICAMENTE CODIFICABLES. SE EMPLEAN COMO ANTICOAGULANTES CON ACTIVIDAD ANTITROMBINA.
ES545994A 1984-08-10 1985-08-08 Procedimiento para preparar un polipeptido con actividad de hirudina Expired ES8605040A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843429430 DE3429430A1 (de) 1984-08-10 1984-08-10 Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens

Publications (2)

Publication Number Publication Date
ES8605040A1 true ES8605040A1 (es) 1986-02-16
ES545994A0 ES545994A0 (es) 1986-02-16

Family

ID=6242746

Family Applications (1)

Application Number Title Priority Date Filing Date
ES545994A Expired ES8605040A1 (es) 1984-08-10 1985-08-08 Procedimiento para preparar un polipeptido con actividad de hirudina

Country Status (18)

Country Link
EP (1) EP0171024B1 (es)
JP (1) JPH0811073B2 (es)
KR (1) KR930009337B1 (es)
AT (1) ATE67245T1 (es)
AU (1) AU584628B2 (es)
CA (1) CA1341124C (es)
DE (2) DE3429430A1 (es)
DK (1) DK175337B1 (es)
ES (1) ES8605040A1 (es)
FI (1) FI91883C (es)
GR (1) GR851962B (es)
HU (1) HU206135B (es)
IE (1) IE58485B1 (es)
IL (1) IL76059A (es)
NO (1) NO177434C (es)
NZ (1) NZ213049A (es)
PT (1) PT80930B (es)
ZA (1) ZA856026B (es)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3438296A1 (de) * 1984-04-18 1985-11-07 Hoechst Ag, 6230 Frankfurt Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
EP0209061B1 (de) * 1985-07-17 1994-01-12 Hoechst Aktiengesellschaft Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel
DE3541856A1 (de) * 1985-11-27 1987-06-04 Hoechst Ag Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
GB2188322A (en) * 1986-03-26 1987-09-30 Bayer Ag Aprotinin and analogues thereof produced by a recombinant host
GB2188933A (en) * 1986-04-10 1987-10-14 Bayer Ag Expression vectors for production of polypeptides, method for enhanced expression of polypeptides, hosts containing the expression vectors, products manufactured thereby
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607516B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
GB2199582A (en) * 1987-01-07 1988-07-13 Bayer Ag Analogues of pancreatic secretory trypsin inhibitor
US5032573A (en) * 1987-03-23 1991-07-16 Bayer Aktiengesellschaft Homologs of aprotinin produced from a recombinant host, process, expression vector and recombinant host therefor and pharmaceutical use thereof
US5236898A (en) * 1987-05-21 1993-08-17 Merrell Dow Pharmaceuticals Inc. Cyclic anticoagulant peptides
US5192745A (en) * 1987-05-21 1993-03-09 Merrell Dow Pharmaceuticals Inc. Cyclic anticoagulant peptides
GB8723662D0 (en) * 1987-10-08 1987-11-11 British Bio Technology Synthetic gene
GB8723661D0 (en) * 1987-10-08 1987-11-11 British Bio Technology Synthetic gene
US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
FR2628429B1 (fr) * 1988-03-08 1990-12-28 Transgene Sa Variants de l'hirudine, leurs utilisations et les procedes pour les obtenir
DE3819079A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Hirudin-derivate mit verzoegerter wirkung
US5167960A (en) * 1988-08-03 1992-12-01 New England Deaconess Hospital Corporation Hirudin-coated biocompatible substance
US5112615A (en) * 1988-08-03 1992-05-12 New England Deaconess Hospital Corporation Soluble hirudin conjugates
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
US5192747A (en) * 1989-10-03 1993-03-09 Merrell Dow Pharmaceuticals Inc. Anticoagulant peptides
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
GB9010552D0 (en) * 1990-05-10 1990-07-04 Erba Carlo Spa Method for the recombinant production of hirudins and novel hirudins
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
DE4140381A1 (de) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet
US5972648A (en) * 1993-09-28 1999-10-26 Japan Energy Corporation Hirudin analogs, methods of manufacture thereof and anticoagulant compositions having these as active ingredients
DE4404168A1 (de) * 1994-02-10 1995-08-17 Hoechst Ag Hirudinderivate und Verfahren zu deren Herstellung
DE19529997C1 (de) 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE19543737A1 (de) 1995-11-24 1997-05-28 Hoechst Ag Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) * 1995-11-28 1997-06-05 Hoechst Ag Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
DE19607239A1 (de) 1996-02-27 1997-08-28 Behringwerke Ag Pharmazeutische Zusammensetzung, enthaltend Hirudin und Verfahren zu deren Herstellung
DE19944870A1 (de) * 1999-09-18 2001-03-29 Aventis Pharma Gmbh Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
DE10149678A1 (de) 2001-10-09 2009-03-12 Novel Science International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
DE10301255A1 (de) 2003-01-15 2004-07-29 Cpi Creative Pharma International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK339781A (da) * 1980-08-05 1982-02-06 Searle & Co Syntetisk gen
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
CA1341414C (fr) * 1984-03-27 2002-12-31 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
ATE64956T1 (de) * 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.

Also Published As

Publication number Publication date
KR930009337B1 (ko) 1993-09-27
HU206135B (en) 1992-08-28
AU584628B2 (en) 1989-06-01
JPS6147198A (ja) 1986-03-07
IL76059A0 (en) 1985-12-31
AU4597785A (en) 1986-02-13
DK364585A (da) 1986-02-11
CA1341124C (en) 2000-10-24
NO853155L (no) 1986-02-11
DE3429430A1 (de) 1986-02-20
FI853056L (fi) 1986-02-11
FI91883C (fi) 1994-08-25
NO177434B (no) 1995-06-06
ATE67245T1 (de) 1991-09-15
PT80930A (de) 1985-09-01
EP0171024A1 (de) 1986-02-12
ZA856026B (en) 1986-03-26
GR851962B (es) 1985-12-03
DK364585D0 (da) 1985-08-09
PT80930B (pt) 1987-11-11
JPH0811073B2 (ja) 1996-02-07
DK175337B1 (da) 2004-08-30
IL76059A (en) 1993-08-18
FI91883B (fi) 1994-05-13
ES545994A0 (es) 1986-02-16
FI853056A0 (fi) 1985-08-08
EP0171024B1 (de) 1991-09-11
IE851973L (en) 1986-02-10
DE3584053D1 (de) 1991-10-17
HUT38968A (en) 1986-07-28
NO177434C (no) 1995-09-13
IE58485B1 (en) 1993-09-22
KR870002257A (ko) 1987-03-30
NZ213049A (en) 1988-05-30

Similar Documents

Publication Publication Date Title
ES8605040A1 (es) Procedimiento para preparar un polipeptido con actividad de hirudina
EP0125023B2 (en) Recombinant immunoglobulin preparations
Oka et al. Synthesis and secretion of human epidermal growth factor by Escherichia coli.
US4888286A (en) Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides
US4994379A (en) Modified signal peptides
AU607209B2 (en) Secretion of insulin-like growth factor-i in e. coli
JPS63501614A (ja) 外来タンパク質を分泌することを目的としたｂａｒ１の使用法
AU591087B2 (en) Chimaeric gene coding for a transit peptide and a heterologous polypeptide
KR880014110A (ko) 인체 프로아포지방단백질 a-i의 발현
ES8604252A1 (es) Un metodo para recuperar una hormona de credimiento de un animal purificada
EP0305488A4 (en) Mycobacterial recombinants and peptides
HUT46061A (en) Dna sequences encoding hirudin-like protein and process for producing such proteins
RU2001110120A (ru) Модифицированный сигнальный пептид энтеротоксина-ii e. coli и микроорганизм, экспрессирующий белок слияния упомянутого пептида с гетерологичным белком
EP0211047A4 (en) ARAB PROMOTERS AND PROCESS FOR THE PRODUCTION OF POLYPEPTIDES, INCLUDING CECROPINS, BY MICROBIOLOGICAL TECHNIQUES.
DE3686579D1 (en) Prokaryotisches expressionssystem.
EP0317641A4 (en) Fused protein containing lymphotoxin
EP0346500A4 (en) Elastase inhibiting polypeptides and process for their preparation by genetic recombination
ES2258797T3 (es) Proteinas con extension n-terminal expresadas en la levadura.
EP0528686B8 (en) Process for producing peptides
CA2315282A1 (en) Single-chain polypeptides comprising troponin i and troponin c
EP0035454A3 (en) Desacetylthymosin alpha 1, its process of preparation and its use
JPH02150285A (ja) バシルス・コアグランスのアミラーゼ遺伝子に基づくバシルス発現ベクター
US20010021515A1 (en) Dnas encoding new fusion proteins and processes for preparing useful polypeptides through expression of the dnas
Takahashi et al. The amino acid sequence of ribonuclease U1, a guanine-specific ribonuclease from the fungus Ustilago sphaerogena
EP0254315A3 (en) Method for producing peptides

Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20051128