FR2607516B2 - Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu - Google Patents

Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu

Info

Publication number
FR2607516B2
FR2607516B2 FR868616722A FR8616722A FR2607516B2 FR 2607516 B2 FR2607516 B2 FR 2607516B2 FR 868616722 A FR868616722 A FR 868616722A FR 8616722 A FR8616722 A FR 8616722A FR 2607516 B2 FR2607516 B2 FR 2607516B2
Authority
FR
France
Prior art keywords
yeast
expressing
product obtained
improved vectors
hirudin variant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
FR868616722A
Other languages
English (en)
Other versions
FR2607516A2 (fr
Inventor
Nathalie Labat
Gerard Loison
Alain Balland
Yves Lemoine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Transgene SA
Original Assignee
Transgene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene SA filed Critical Transgene SA
Priority to FR868616722A priority Critical patent/FR2607516B2/fr
Priority to MC871896A priority patent/MC1834A1/fr
Priority to AU75366/87A priority patent/AU614933B2/en
Priority to PT85296A priority patent/PT85296B/pt
Priority to SU874202962A priority patent/RU1776276C/ru
Priority to CA000541712A priority patent/CA1341490C/fr
Priority to DK198703558A priority patent/DK175195B1/da
Priority to JP62173724A priority patent/JP2733602B2/ja
Priority to NO872878A priority patent/NO179489C/no
Priority to ES87401649T priority patent/ES2103700T3/es
Priority to AT87401649T priority patent/ATE153066T1/de
Priority to PL1987266756A priority patent/PL154118B1/pl
Priority to CS875277A priority patent/CZ278268B6/cs
Priority to HU873161A priority patent/HU214236B/hu
Priority to DE3752063T priority patent/DE3752063T2/de
Priority to KR1019870007450A priority patent/KR960013464B1/ko
Priority to EP87401649A priority patent/EP0252854B1/fr
Priority to FI873068A priority patent/FI96966C/fi
Publication of FR2607516A2 publication Critical patent/FR2607516A2/fr
Application granted granted Critical
Publication of FR2607516B2 publication Critical patent/FR2607516B2/fr
Priority to US08/405,765 priority patent/US5591640A/en
Priority to US08/708,543 priority patent/US5902735A/en
Priority to GR970402015T priority patent/GR3024368T3/el
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR868616722A 1986-07-10 1986-12-01 Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu Expired FR2607516B2 (fr)

Priority Applications (21)

Application Number Priority Date Filing Date Title
FR868616722A FR2607516B2 (fr) 1986-12-01 1986-12-01 Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
MC871896A MC1834A1 (fr) 1986-07-10 1987-07-02 Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
AU75366/87A AU614933B2 (en) 1986-07-10 1987-07-08 Functional DNA block and plasmid coding for hirudin, transformed yeast, method for preparing hirudin, hirudin obtained and its pharmaceutical use
SU874202962A RU1776276C (ru) 1986-07-10 1987-07-09 Способ получени полипептида со свойствами гирудина
CA000541712A CA1341490C (fr) 1986-07-10 1987-07-09 Bloc fonctionnel d'adn et plasmide codant pour l'hirudine, levure transformee procede de preparation de l'hirudine, hirudine obtenue et son utilisation pharmaceutique
DK198703558A DK175195B1 (da) 1986-07-10 1987-07-09 Hirudinekspression
PT85296A PT85296B (pt) 1986-07-10 1987-07-09 Bloco funcional de adn, e plasmideo que codifica para a hirudina, e respectivo processo de preparacao
EP87401649A EP0252854B1 (fr) 1986-07-10 1987-07-10 Bloc fonctionnel d'ADN et plasmide codant pour l'hirudine, levure transformée et procédé de préparation de l'hirudine.
ES87401649T ES2103700T3 (es) 1986-07-10 1987-07-10 Bloque funcional de a.d.n. y plasmido que codifica para la hirudina, levadura transformada y procedimiento para la preparacion de hirudina.
AT87401649T ATE153066T1 (de) 1986-07-10 1987-07-10 Funktioneller dns-block und für hirudin kodierendes plasmid, transformierte hefe aud verfahren zur herstellung von hirudin.
JP62173724A JP2733602B2 (ja) 1986-07-10 1987-07-10 ヒルジンをコードする発現カセット、プラスミド及び形質転換酵母
CS875277A CZ278268B6 (en) 1986-07-10 1987-07-10 Process for preparing hirudin
HU873161A HU214236B (hu) 1986-07-10 1987-07-10 Eljárás hirudin és hirudinvariánsok előállítására
DE3752063T DE3752063T2 (de) 1986-07-10 1987-07-10 Funktioneller DNS-Block und für Hirudin kodierendes Plasmid, transformierte Hefe aud Verfahren zur Herstellung von Hirudin.
KR1019870007450A KR960013464B1 (ko) 1986-07-10 1987-07-10 기능성 dna 블록, 이를 함유한 플라즈미드, 플라즈미드에 의해 형질 전환된 효모, 히루딘의 제조방법 및 용도
NO872878A NO179489C (no) 1986-07-10 1987-07-10 Ekspresjonskassett, plasmid inneholdende ekspresjonskassetten, transformert gjærcelle og fremgangsmåte til fremstilling av en hirudinvariant
FI873068A FI96966C (fi) 1986-07-10 1987-07-10 Ilmentämiskasetti hirudiinivariantin tuottamiseksi hiivassa, sitä sisältävä plasmidi, sillä transformoitu hiivasolu ja menetelmä hirudiinivariantin valmistamiseksi hiivasolun avulla
PL1987266756A PL154118B1 (en) 1986-07-10 1987-07-10 Method for manufacturing hirudine
US08/405,765 US5591640A (en) 1986-07-10 1995-03-16 Functional DNA block and plasmid coding for hirudin, transformed yeast, method for preparing hirudin, hirudin obtained and its pharmaceutical use
US08/708,543 US5902735A (en) 1986-07-10 1996-09-05 Functional DNA block and plasmid coding for hirudin, transformed yeast, method for hirudin, hirudin obtained, and its pharmaceutical use
GR970402015T GR3024368T3 (en) 1986-07-10 1997-08-06 Functional DNA block and plasmid coding for hirudine, transformed yeast and process for the preparation of hirudine.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR868616722A FR2607516B2 (fr) 1986-12-01 1986-12-01 Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu

Publications (2)

Publication Number Publication Date
FR2607516A2 FR2607516A2 (fr) 1988-06-03
FR2607516B2 true FR2607516B2 (fr) 1989-12-22

Family

ID=9341392

Family Applications (1)

Application Number Title Priority Date Filing Date
FR868616722A Expired FR2607516B2 (fr) 1986-07-10 1986-12-01 Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu

Country Status (1)

Country Link
FR (1) FR2607516B2 (fr)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341417C (fr) * 1984-03-27 2003-01-21 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
ATE64956T1 (de) * 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees

Also Published As

Publication number Publication date
FR2607516A2 (fr) 1988-06-03

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