DK496886A - Udtrykkelse og sekretion af heterologe proteiner ved hjaelp af yarrowia lipolytica transformanter - Google Patents

Udtrykkelse og sekretion af heterologe proteiner ved hjaelp af yarrowia lipolytica transformanter Download PDF

Info

Publication number
DK496886A
DK496886A DK496886A DK496886A DK496886A DK 496886 A DK496886 A DK 496886A DK 496886 A DK496886 A DK 496886A DK 496886 A DK496886 A DK 496886A DK 496886 A DK496886 A DK 496886A
Authority
DK
Denmark
Prior art keywords
lipolytica
heterologous
xpr2
expression
vectors
Prior art date
Application number
DK496886A
Other languages
Danish (da)
English (en)
Other versions
DK496886D0 (da
Inventor
Lance Steven Davidow
John Robert Dezeeuw
Arthur Ernest Franke
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of DK496886D0 publication Critical patent/DK496886D0/da
Publication of DK496886A publication Critical patent/DK496886A/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/472Complement proteins, e.g. anaphylatoxin, C3a, C5a
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/55Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Magnetic Ceramics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Treatment Of Sludge (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Paper (AREA)
  • Fodder In General (AREA)
  • Noodles (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Measurement Of Velocity Or Position Using Acoustic Or Ultrasonic Waves (AREA)
DK496886A 1985-10-18 1986-10-17 Udtrykkelse og sekretion af heterologe proteiner ved hjaelp af yarrowia lipolytica transformanter DK496886A (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US78920685A 1985-10-18 1985-10-18
US06/841,121 US4937189A (en) 1985-10-18 1986-03-18 Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants

Publications (2)

Publication Number Publication Date
DK496886D0 DK496886D0 (da) 1986-10-17
DK496886A true DK496886A (da) 1987-06-11

Family

ID=27120892

Family Applications (1)

Application Number Title Priority Date Filing Date
DK496886A DK496886A (da) 1985-10-18 1986-10-17 Udtrykkelse og sekretion af heterologe proteiner ved hjaelp af yarrowia lipolytica transformanter

Country Status (24)

Country Link
US (1) US4937189A (no)
EP (1) EP0220864B1 (no)
JP (1) JP3096297B2 (no)
KR (1) KR900008950B1 (no)
CN (3) CN1034428C (no)
AT (1) ATE86302T1 (no)
AU (1) AU586041B2 (no)
CA (1) CA1340837C (no)
DD (7) DD276886A5 (no)
DE (1) DE3687878T2 (no)
DK (1) DK496886A (no)
EG (1) EG18142A (no)
ES (1) ES2038966T3 (no)
FI (1) FI90352C (no)
HU (1) HU209150B (no)
IE (1) IE59774B1 (no)
IL (7) IL99894A (no)
NO (2) NO177318C (no)
NZ (1) NZ217985A (no)
PH (3) PH27026A (no)
PL (2) PL155850B1 (no)
PT (1) PT83562B (no)
RU (2) RU2069694C1 (no)
YU (2) YU46412B (no)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH082304B2 (ja) * 1987-12-17 1996-01-17 オリエンタル酵母工業株式会社 プラスミドとその製造方法
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
EP0382332A3 (en) * 1989-01-06 1990-11-14 Collaborative Research Inc. Improved supersecreting mutants of saccharomyces cerevisiae
JPH0669365B2 (ja) * 1989-05-22 1994-09-07 株式会社ミドリ十字 アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法
EP0402226A1 (en) * 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
US5786212A (en) * 1993-09-02 1998-07-28 Pfizer Inc. Multiple integrative vectors and Yarrowia lipolytica transformants
US5807824A (en) * 1993-12-06 1998-09-15 Ciba-Geigy Corporation C5A receptor antagonists having substantially no agonist activity
US5837499A (en) * 1993-12-06 1998-11-17 Ciba-Geigy Corporation DNA encoding C5A receptor antagonists having substantially no agonist activity and methods of expressing same
DE19513152A1 (de) * 1995-04-07 1996-10-10 Bundesrep Deutschland Verwendung eines "Immundefizienzvirus-supprimierenden Lymphokins (ISL)" zur Hemmung der Virusvermehrung, insbesondere von Retroviren
WO1996039503A1 (en) * 1995-06-05 1996-12-12 Novartis Ag C5a RECEPTOR ANTAGONISTS HAVING SUBSTANTIALLY NO AGONIST ACTIVITY AND METHODS FOR PREPARATION
EP0747484A1 (en) 1995-06-08 1996-12-11 Institut National De La Recherche Agronomique (Inra) Upstream activator sequences and recombinant promoter sequences functional in yarrowia and vectors containing them
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
FR2782733B1 (fr) 1998-09-01 2002-02-08 Agronomique Inst Nat Rech Procede de transformation non-homologue de yarrowia lipolytica
US20110059496A1 (en) * 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7259255B2 (en) * 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7459546B2 (en) 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7202356B2 (en) * 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
US7264949B2 (en) * 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US20060094102A1 (en) * 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
UA94038C2 (ru) 2005-03-18 2011-04-11 Майкробиа, Инк. Продуцирование каротиноидов в маслянистых дрожжах и грибах
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
ES2575008T3 (es) * 2008-07-11 2016-06-23 Nstitut National De La Recherche Agronomique (Inra) Nuevas cepas de levadura mutantes capaces de acumular una gran cantidad de lípidos
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
KR20140000711A (ko) 2010-12-30 2014-01-03 이 아이 듀폰 디 네모아 앤드 캄파니 수크로스 이용에 있어서의 야로위아 리포라이티카에서의 사카로마이세스 세레비지애 suc2 유전자의 용도
US8969049B2 (en) 2011-03-31 2015-03-03 E I Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
US8609369B2 (en) 2011-04-07 2013-12-17 E I Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-CoA reductase promoter regions for gene expression in yeast
GB201106188D0 (en) * 2011-04-12 2011-05-25 Csir Production of extracellular polypeptides
FR3002774A1 (fr) 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
FR3005317B1 (fr) 2013-05-02 2016-03-18 Agronomique Inst Nat Rech Levures mutantes capables de produire un acide gras inhabituel
WO2015116781A2 (en) 2014-01-31 2015-08-06 Dsm Ip Assets B.V. Promoters suitable for heterologous gene expression in yeast
EP3155094A1 (en) 2014-06-11 2017-04-19 Institut National De La Recherche Agronomique (INRA) Improved lipid accumulation in yarrowia lipolytica strains by overexpression of hexokinase and new strains thereof
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
US20180362961A1 (en) 2015-12-11 2018-12-20 Danisco Us Inc. Methods and compositions for enhanced nuclease-mediated genome modification and reduced off-target site effects
DK3390631T3 (da) 2015-12-18 2020-07-13 Danisco Us Inc Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
EP3455362A1 (en) 2016-05-10 2019-03-20 Institut National De La Recherche Agronomique Mutant yeast strains with enhanced production of erythritol or erythrulose
EP3348647A1 (en) 2017-01-13 2018-07-18 Institut National De La Recherche Agronomique Mutant yeast strain capable of producing medium chain fatty acids

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4321365A (en) * 1977-10-19 1982-03-23 Research Corporation Oligonucleotides useful as adaptors in DNA cloning, adapted DNA molecules, and methods of preparing adaptors and adapted molecules
US4394443A (en) * 1980-12-18 1983-07-19 Yale University Method for cloning genes
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4628033A (en) * 1983-10-06 1986-12-09 Pfizer Inc. Novel host strain for transformation of Yarrowia lipolytica
IN163393B (no) * 1983-10-06 1988-09-17 Pfizer
EP0166659B1 (fr) * 1984-06-26 1989-08-30 Institut National De La Recherche Agronomique (Inra) Vecteurs de transformation de la levure yarrowia lipolytica, procédé de transformation et levure transformée

Also Published As

Publication number Publication date
FI864206A0 (fi) 1986-10-17
DE3687878T2 (de) 1993-07-15
NO177318C (no) 1995-08-23
DD267996A5 (de) 1989-05-17
NO177318B (no) 1995-05-15
IE862754L (en) 1987-04-18
AU586041B2 (en) 1989-06-29
IL99893A0 (en) 1992-08-18
YU177386A (en) 1989-04-30
PH27198A (en) 1993-04-16
YU46412B (sh) 1993-10-20
YU46571B (sh) 1993-11-16
KR870004138A (ko) 1987-05-07
HU209150B (en) 1994-03-28
IL80297A (en) 1993-02-21
NO178793C (no) 1996-06-05
EP0220864B1 (en) 1993-03-03
EP0220864A1 (en) 1987-05-06
IL99892A (en) 1993-02-21
IL99894A0 (en) 1992-08-18
CN86106522A (zh) 1987-09-16
PT83562B (pt) 1989-05-31
DD269396A5 (de) 1989-06-28
CN1069346C (zh) 2001-08-08
DD269397A5 (de) 1989-06-28
JP3096297B2 (ja) 2000-10-10
IL99894A (en) 1993-02-21
IL99893A (en) 1993-02-21
NO864148D0 (no) 1986-10-17
RU2069694C1 (ru) 1996-11-27
NO923616D0 (no) 1992-09-17
ATE86302T1 (de) 1993-03-15
NZ217985A (en) 1991-06-25
FI864206A (fi) 1987-04-19
IL99892A0 (en) 1992-08-18
PL261912A1 (en) 1987-10-19
DD267995A5 (de) 1989-05-17
IE59774B1 (en) 1994-04-06
DE3687878D1 (de) 1993-04-08
PL155850B1 (en) 1992-01-31
US4937189A (en) 1990-06-26
DD267997A5 (de) 1989-05-17
YU124588A (en) 1989-08-31
RU2157845C2 (ru) 2000-10-20
ES2038966T3 (es) 1993-08-16
NO923616L (no) 1987-04-21
IL80297A0 (en) 1987-01-30
PH27026A (en) 1993-02-01
CA1340837C (en) 1999-12-07
DD276886A5 (de) 1990-03-14
EG18142A (en) 1992-08-30
DK496886D0 (da) 1986-10-17
CN1141339A (zh) 1997-01-29
CN1034428C (zh) 1997-04-02
NO178793B (no) 1996-02-26
CN1138632A (zh) 1996-12-25
PL154427B1 (en) 1991-08-30
FI90352B (fi) 1993-10-15
HUT43632A (en) 1987-11-30
AU6416186A (en) 1987-05-14
PT83562A (en) 1986-11-01
JPS63164889A (ja) 1988-07-08
FI90352C (fi) 1994-01-25
KR900008950B1 (ko) 1990-12-15
DD267998A5 (de) 1989-05-17
PH27171A (en) 1993-04-02
NO864148L (no) 1987-04-21

Similar Documents

Publication Publication Date Title
DK496886A (da) Udtrykkelse og sekretion af heterologe proteiner ved hjaelp af yarrowia lipolytica transformanter
IL80022A (en) Cloned human basic fibroblast growth factor, methods for its preparation, compositions containing it, dna sequences encoding therefor and vectors and host cells containing said dna
ATE46536T1 (de) Verfahren zur herstellung von reifen proteinen in wirtzellen von wirbeltieren.
EP0196864A3 (en) Alkaline phosphatase-mediated processing and secretion of recombinant proteins, dna sequences for use therein and cells transformed using such sequences
SE7811461L (sv) Forfarande och medel for polypeptidbildning via mikrober
EP0260148A3 (en) Improved recombinant expression method, vector and transformed cells
DE3072070D1 (en) Plasmid vectors, plasmids containing genes for alkaline phosphatases and bacteria containing the latter, their preparation and use
EP0206783A3 (en) Expression and secretion of polypeptides from saccharomyces cerevisiae
FR2724183B1 (fr) Vecteurs de criblage et/ou d'expression fonctionnels chez les mycobacteries - utilisation pour l'identification et l'expression de polypeptides exportes
EP0266057A3 (en) Plasmid containing an eucaryotic secretory signal sequence and process for secreting encoded proteins
ES8800721A1 (es) Un metodo para producir un granulo intracelular de la hormona bovina del crecimiento, altamente homogeneo.
KR910000180A (ko) 트롬빈 억제 활성을 갖는 폴리펩티드 및 그의 제조방법
RU92004585A (ru) Промотор, днк-последовательность, вектор, клетки, способ экспрессии днк
ATE154392T1 (de) Kalzium-geregelter promotor

Legal Events

Date Code Title Description
AHS Application shelved for other reasons than non-payment