CY1118000T1 - Διαδικασια υψηλης παροχης για αναλυση μεθυλιωσης dna - Google Patents

Διαδικασια υψηλης παροχης για αναλυση μεθυλιωσης dna

Info

Publication number
CY1118000T1
CY1118000T1 CY20161100185T CY161100185T CY1118000T1 CY 1118000 T1 CY1118000 T1 CY 1118000T1 CY 20161100185 T CY20161100185 T CY 20161100185T CY 161100185 T CY161100185 T CY 161100185T CY 1118000 T1 CY1118000 T1 CY 1118000T1
Authority
CY
Cyprus
Prior art keywords
nucleic acid
genomic dna
dna samples
specifically
dna methylation
Prior art date
Application number
CY20161100185T
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English (en)
Inventor
Peter W Laird
Cindy A Carroll
Kathleen D Danenberg
Original Assignee
University Of Southern California
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23209034&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1118000(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University Of Southern California filed Critical University Of Southern California
Publication of CY1118000T1 publication Critical patent/CY1118000T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Κοινοποιείται μία ποσοτική διαδικασία υψηλής παροχής για τον προσδιορισμό των μοτίβων μεθυλίωσης σε δείγματα γονιδιωματικού DNA που βασίζεται σε ενίσχυση τροποποιημένου νουκλεϊκου (nucleic)οξέος, και ανίχνευση μεθυλιωμένου νουκλεϊκου (nucleic) οξέος που βασίζεται σε μετατόπιση εξαρτώμενη από την ενίσχυση ειδικά ανοπτημένων αισθητηρίων υβριδοποίησης. Ειδικότερα η διαδικασία της εφεύρεσης παρέχει επεξεργασία δειγμάτων γονιδιωματικού DNA με όξινο θειώδες νάτριο για την δημιουργία διαφορών αλληλουχίας που εξαρτώνται από την μεθυλίωση, που ακολουθείται από ανίχνευση με τεχνικές ποσοτικής PCR που βασίζεται σε φθορισμό. Η διαδικασία είναι ιδιαίτερα κατάλληλη για ταχεία ανάλυση μεγάλου αριθμού δειγμάτων νουκλεϊκού (nuclei)οξέος, όπως αυτά από συλλογές ιστών όγκου.
CY20161100185T 1999-05-14 2016-03-03 Διαδικασια υψηλης παροχης για αναλυση μεθυλιωσης dna CY1118000T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/311,912 US6331393B1 (en) 1999-05-14 1999-05-14 Process for high-throughput DNA methylation analysis
EP00928969.5A EP1185695B2 (en) 1999-05-14 2000-05-12 Process for high throughput dna methylation analysis

Publications (1)

Publication Number Publication Date
CY1118000T1 true CY1118000T1 (el) 2017-05-17

Family

ID=23209034

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20161100185T CY1118000T1 (el) 1999-05-14 2016-03-03 Διαδικασια υψηλης παροχης για αναλυση μεθυλιωσης dna

Country Status (11)

Country Link
US (4) US6331393B1 (el)
EP (2) EP1710321B1 (el)
JP (2) JP4044290B2 (el)
AT (1) ATE332980T2 (el)
AU (2) AU775798B2 (el)
CA (1) CA2372665C (el)
CY (1) CY1118000T1 (el)
DE (1) DE60029323T3 (el)
DK (2) DK1185695T4 (el)
ES (2) ES2564048T3 (el)
WO (1) WO2000070090A1 (el)

Families Citing this family (256)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US7611869B2 (en) * 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US8076063B2 (en) 2000-02-07 2011-12-13 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
JP2004505612A (ja) * 2000-03-31 2004-02-26 ユニバーシティ・オブ・サザン・カリフォルニア 食道腺ガンに関する後成的配列
JP2004508807A (ja) * 2000-04-06 2004-03-25 エピゲノミクス　アーゲー 遺伝子調節に関連する疾患の診断
DE60125065T2 (de) * 2000-08-25 2007-06-28 Lovelace Respiratory Research Institute, Albuquerque Verfahren zur krebsdetektion mittels verschachtelter ("nested") methylierungsspezifischer pcr
US20020058265A1 (en) * 2000-09-15 2002-05-16 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US6844152B1 (en) * 2000-09-15 2005-01-18 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
WO2002034942A2 (en) * 2000-10-23 2002-05-02 Cancer Research Technology Limited Nucleic acid amplification-based methods for the determination of a methylation profile and reagents therefor
GB0025913D0 (en) 2000-10-23 2000-12-06 Guldberg Per Materials and methods relating to nucleic acid amplification and profiling
US20030188326A1 (en) 2000-11-03 2003-10-02 Dana Farber Cancer Institute Methods and compositions for the diagnosis of cancer susceptibilities and defective DNA repair mechanisms and treatment thereof
DE10056802B4 (de) * 2000-11-14 2005-06-16 Epigenomics Ag Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik
DE10104937B4 (de) * 2001-01-29 2005-03-17 Epigenomics Ag Fluoreszenzpolarisation 2
US6893820B1 (en) * 2001-01-31 2005-05-17 The Ohio State University Research Foundation Detection of methylated CpG rich sequences diagnostic for malignant cells
EP1364069B1 (en) * 2001-03-01 2009-04-22 Epigenomics AG Method for the development of gene panels for diagnostic and therapeutic purposes based on the expression and methylatoin status of the genes
EP1379694B1 (en) * 2001-03-01 2006-05-10 Epigenomics AG Methods and computer program products for determining the biological effect and/or activity of drugs, chemical substances and/or pharmaceutical compositions based on their effect on the methylation status of the dna
DE10112515B4 (de) * 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US20020197639A1 (en) * 2001-06-08 2002-12-26 Shia Michael A. Methods and products for analyzing nucleic acids based on methylation status
DE10128508A1 (de) 2001-06-14 2003-02-06 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren
DE10128509A1 (de) * 2001-06-14 2003-01-02 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata- und Nierenkarzinomen
DE10130800B4 (de) 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
DE10132211A1 (de) * 2001-06-27 2003-01-16 Epigenomics Ag Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET)
US8207142B2 (en) 2001-07-31 2012-06-26 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Inhibitor of DNA methylation
DE10139283A1 (de) 2001-08-09 2003-03-13 Epigenomics Ag Verfahren und Nukleinsäuren zur Analyse von Colon-Krebs
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
AU2002352745A1 (en) * 2001-11-16 2003-06-10 The Johns Hopkins University School Of Medicine Method of detection of prostate cancer
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2467814A1 (en) * 2001-12-03 2003-06-12 Illumina, Inc. Multiplexed methylation detection methods
AU2003212878A1 (en) * 2002-01-30 2003-09-02 Epigenomics Ag Identification of cell differentiation states based on methylation patterns
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
AU2003245465A1 (en) * 2002-02-13 2003-09-04 Medimolecular Pty. Ltd. Method of isolating nucleic acids from stool samples
KR100470221B1 (ko) * 2002-02-20 2005-02-05 굿젠 주식회사 유전자 프로모터 ＣｐＧ 아일랜드의 메틸화 여부를분석하는 염기서열분석형 올리고뉴클레오타이드칩, 이의제조방법 및 이를 이용한 암 검색방법
EP1340818A1 (en) * 2002-02-27 2003-09-03 Epigenomics AG Method and nucleic acids for the analysis of a colon cell proliferative disorder
DE60207979T2 (de) * 2002-03-05 2006-09-28 Epigenomics Ag Verfahren und Vorrichtung zur Bestimmung der Gewebespezifität von freier DNA in Körperflüssigkeiten
US20060099581A1 (en) * 2002-04-09 2006-05-11 Kurt Berlin Method for analysis of methylated nucleic acids
AU2003244135A1 (en) * 2002-06-21 2004-01-06 Hinode Co, . Ltd. Device for removing raindrops on umrella and system for promoting installation of the device
US7186512B2 (en) * 2002-06-26 2007-03-06 Cold Spring Harbor Laboratory Methods and compositions for determining methylation profiles
WO2004013284A2 (en) * 2002-08-02 2004-02-12 Tufts University A method to assess genomic dna methylation using high-performance liquid chromatography-electospray ionization mass spectrometry
DE10236406C1 (de) * 2002-08-02 2003-12-24 Epigenomics Ag Verfahren zur Amplifikation von Nukleinsäuren mit geringer Komplexität
AU2003236461B2 (en) * 2002-08-29 2009-05-28 Epigenomics Ag Improved method for bisulfite treatment
EP1552001A4 (en) * 2002-09-19 2006-01-04 Applera Corp METHODS AND COMPOSITIONS FOR TARGET DETECTION
EP2157191B1 (en) * 2002-10-01 2013-12-25 Epigenomics AG Use of PITX2 nucleic acids for the improved treatment of breast cell proliferative disorders
CA2442438C (en) 2002-10-04 2012-06-19 Nisshinbo Industries, Inc. Oligonucleotide-immobilized substrate for detecting methylation
US8039218B2 (en) * 2002-11-14 2011-10-18 John Wayne Cancer Institute Detection of cancer cells in body fluids
EP1572105B1 (en) * 2002-11-14 2011-08-31 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative rt-pcr
US20040101843A1 (en) * 2002-11-22 2004-05-27 Gerald Zon Detection of methylated DNA sites
EP1567669B1 (en) * 2002-12-02 2010-03-24 Illumina Cambridge Limited Determination of methylation of nucleic acid sequences
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
JP2006516391A (ja) * 2003-01-29 2006-07-06 エフ．ホフマン−ラ　ロシュ　アーゲー 重亜硫酸塩処理の改良された方法
DE10304219B3 (de) * 2003-01-30 2004-08-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US20090305294A1 (en) * 2003-02-13 2009-12-10 Medimolecular Pty Ltd Method of isolating nucleic acids from stool samples
WO2004087957A2 (en) * 2003-04-03 2004-10-14 Oncomethylome Sciences S.A. Hypermethylated genes and cervical cancer
US20060024676A1 (en) * 2003-04-14 2006-02-02 Karen Uhlmann Method of detecting epigenetic biomarkers by quantitative methyISNP analysis
US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US8150626B2 (en) * 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US20050026183A1 (en) * 2003-05-15 2005-02-03 Jian-Bing Fan Methods and compositions for diagnosing conditions associated with specific DNA methylation patterns
DE602004028478D1 (de) * 2003-06-17 2010-09-16 Human Genetic Signatures Pty Verfahren zur genomamplifikation
DE602004030910D1 (de) * 2003-06-23 2011-02-17 Epigenomics Ag Verfahren und nukleinsäuren zur analyse von störungen der proliferation von kolonzellen
CA2531451A1 (en) 2003-06-23 2005-01-06 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
US20040265833A1 (en) * 2003-06-23 2004-12-30 Cathy Lofton-Day Methods and nucleic acids for the analysis of colorectal cell proliferative disorders
AU2004252554B2 (en) 2003-06-23 2012-01-19 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
US20070117093A1 (en) * 2003-06-24 2007-05-24 Reimo Tetzner Heavymethyl assay for the methylation analysis of the gstpi gene
EP1651778A1 (en) * 2003-08-01 2006-05-03 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
GB0318110D0 (en) * 2003-08-01 2003-09-03 Isaeo Ltd Methods and kits for detecting an enzyme capable of modifying a nucleic acid
DE10338308B4 (de) * 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
DE602004018801D1 (de) * 2003-09-04 2009-02-12 Human Genetic Signatures Pty Nukleinsäurenachweistest
US7655399B2 (en) * 2003-10-08 2010-02-02 Trustees Of Boston University Methods for prenatal diagnosis of chromosomal abnormalities
CA2540310A1 (en) 2003-10-09 2005-04-28 Epigenomics Ag Improved bisulfite conversion of dna
US7901880B2 (en) * 2003-10-21 2011-03-08 Orion Genomics Llc Differential enzymatic fragmentation
CA2548528A1 (en) 2003-12-01 2005-06-16 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
WO2005054502A1 (en) 2003-12-02 2005-06-16 Roche Diagnostics Gmbh Improved method for bisulfite treatment
EP1561821B1 (en) 2003-12-11 2011-02-16 Epigenomics AG Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
CA2487578A1 (en) * 2003-12-11 2005-06-11 Epigenomics Ag Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
EP1718766A4 (en) * 2004-02-10 2009-05-27 Cornell Res Foundation Inc METHOD FOR DETECTING PROMOTER METHYLATION STATE
US7459274B2 (en) * 2004-03-02 2008-12-02 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
JP2007530051A (ja) * 2004-03-24 2007-11-01 アプレラ コーポレイション 標的分子を決定するためのライゲーション反応および増幅反応
EP1655377A1 (de) * 2004-04-06 2006-05-10 Epigenomics AG Verfahren zur Quantifizierung methylierter DNA
US20050287553A1 (en) * 2004-04-06 2005-12-29 Epigenomics Ag Method for the quantification of methylated DNA
US20050233340A1 (en) * 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2005108618A2 (en) * 2004-04-30 2005-11-17 Applera Corporation Methods and kits for methylation detection
US7427479B2 (en) * 2004-04-30 2008-09-23 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
JPWO2005116254A1 (ja) * 2004-05-25 2008-04-03 財団法人ヒューマンサイエンス振興財団 Ｄｎａのメチル化を定量的に評価する方法
US7943308B2 (en) * 2004-06-23 2011-05-17 Epigenomics Ag Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders
EP1609873B1 (en) * 2004-06-23 2010-12-08 Epigenomics AG Method for quantification of methylated DNA
US20060234252A1 (en) * 2004-07-02 2006-10-19 Applera Corporation Methods and kits for methylation detection
EP1985715A3 (en) * 2004-07-16 2008-12-31 Oncomethylome Sciences, S.A. ESR1 and cervical cancer
EP1961827A3 (en) 2004-07-18 2008-09-17 Epigenomics AG Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
EP1632578A1 (en) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
DE602005022740D1 (de) * 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
WO2006034009A2 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
EP1812589A2 (en) * 2004-09-30 2007-08-01 Epigenomics AG Epigenetic methods and nucleic acids for the detection of lung cell proliferative disorders
US7658288B2 (en) * 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP2410064A1 (en) * 2004-11-10 2012-01-25 University Of Southern California High throughput methods comprising analysis of repetitive element DNA methylation
EP2311984A1 (en) 2004-12-02 2011-04-20 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders
CN101111606B (zh) * 2004-12-03 2012-05-16 人类遗传标记控股有限公司 通过化学修饰胞嘧啶而简化微生物核酸的方法
US20080118926A1 (en) 2004-12-13 2008-05-22 National University Corporation Okayama University Method For Detecting Methylation In Genes And Method For Examining Neoplasm Through Detecting Methylation In Genes
US20060134650A1 (en) * 2004-12-21 2006-06-22 Illumina, Inc. Methylation-sensitive restriction enzyme endonuclease method of whole genome methylation analysis
EP1828414A4 (en) * 2004-12-23 2008-12-10 Human Genetic Signatures Pty DETECTION OF HUMAN PAPILLOMA VIRUS
CA2599055C (en) * 2005-02-14 2016-01-12 The Johns Hopkins University Neoplasia screening compositions and methods of use
US20060188910A1 (en) * 2005-02-18 2006-08-24 Applera Corporation Compositions, methods, and kits for analyzing DNA methylation
EP2385136A1 (en) 2005-04-01 2011-11-09 Epigenomics AG Improved bisulfite conversion of DNA
WO2006113466A2 (en) 2005-04-15 2006-10-26 Epigenomics Ag. Methods and nucleic acids for analyses of cellular proliferative disorders
WO2006119434A2 (en) * 2005-05-02 2006-11-09 University Of Southern California DNA METHYLATION MARKERS ASSOCIATED WITH THE CpG ISLAND METHYLATOR PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER
KR100673811B1 (ko) 2005-05-11 2007-01-24 한국표준과학연구원 유전자 내 메틸 시토신의 정량 분석방법 및 이의 사용용도
JP2008541705A (ja) * 2005-05-26 2008-11-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 非標準塩基を含むプライマーを使用する等温鎖置換増幅
WO2006131391A1 (en) * 2005-06-10 2006-12-14 Epigenomics Ag Prognostic assay for prediction of treatment response and/or survival of breast cell proliferative disorder patients
US20070037184A1 (en) * 2005-06-16 2007-02-15 Applera Corporation Methods and kits for evaluating dna methylation
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP1904649A2 (en) * 2005-07-18 2008-04-02 Epigenomics AG Compositions and methods for cancer diagnostics comprising pan-cancer markers
DE502005008746D1 (de) * 2005-07-21 2010-02-04 Epigenomics Ag Verfahren zur Quantifizierung methylierter DNA
ATE531820T1 (de) * 2005-09-14 2011-11-15 Human Genetic Signatures Pty Gesundheitszustandstest
EP1945806B1 (en) 2005-09-21 2013-02-27 Epigenomics AG Markers for the prediction of outcome of anthracycline treatment
US20090191548A1 (en) 2005-09-29 2009-07-30 Epigenomics Ag Methods and nucleic acids for the analysis of gene expression associated with tissue classification
EP1943356B1 (en) * 2005-10-03 2014-05-14 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the prognosis of cell proliferative disorders
US20090042195A1 (en) * 2005-10-07 2009-02-12 Bradford Coffee Methods and systems for screening for and diagnosing dna methylation associated abnormalities and sex chromosome aneuploidies
WO2007042041A1 (en) * 2005-10-12 2007-04-19 Scanvaegt International A/S Device for transfer of items
US20070087358A1 (en) * 2005-10-19 2007-04-19 Melanie Ehrlich Methods for diagnosing cancer based on DNA methylation status in NBL2
CA2629008A1 (en) 2005-11-08 2007-09-13 Euclid Diagnostics Llc Materials and methods for assaying for methylation of cpg islands associated with genes in the evaluation of cancer
EP3095878A1 (en) 2005-11-17 2016-11-23 Epigenomics AG Method for the determination of the dna methylation level of a cpg position in identical cells within a tissue sample
EP1963352B1 (en) * 2005-12-14 2010-09-08 Roche Diagnostics GmbH New method for bisulfite treatment
CA2629824A1 (en) * 2005-12-16 2007-06-21 University Of Maryland, Baltimore Methylation of gene promoters as a predictor of effectiveness of therapy
DE102006001161A1 (de) 2006-01-06 2007-07-12 Qiagen Gmbh Verfahren zum Nachweis von Cytosin-Methylierungen
WO2007088744A1 (ja) * 2006-02-03 2007-08-09 The University Of Tokyo ゲノムdnaのメチル化状況に基づく細胞の同定方法
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
EP1840224A1 (en) 2006-03-29 2007-10-03 Pangaea Biotech, S.A. Method of predicting survival of a non-small-cell lung cancer patient to a chemotherapeutic treatment
EP2011068A4 (en) * 2006-03-30 2010-06-23 Univ Maryland METHYLATION OF GENES AS A PREDICTOR FOR POLYPENE AND REZIDIV
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US20090317810A1 (en) * 2006-04-17 2009-12-24 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
ES2587068T3 (es) 2006-04-17 2016-10-20 Epigenomics Ag Métodos y ácidos nucleicos para la detección de desórdenes celulares proliferativos colorrectales
US20080003609A1 (en) * 2006-05-10 2008-01-03 The Cleveland Clinic Foundation Method of detecting bladder urothelial carcinoma
KR100761031B1 (ko) 2006-05-27 2007-10-04 김연수 디엔에이 메틸화 현상을 이용한 암 진단용 키트
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
EP2041574B1 (en) 2006-07-14 2016-11-16 The Regents of The University of California Cancer biomarkers and methods of use threof
WO2008011620A2 (en) * 2006-07-21 2008-01-24 Epigenomics Ag Methods and nucleic acids for analyses of cellular proliferative disorders
EP2049681B1 (en) * 2006-07-21 2016-09-21 Epigenomics AG Methods for analyses of cellular proliferative disorders
CA2660218A1 (en) * 2006-08-08 2008-02-14 Epigenomics Ag A method for methylation analysis of nucleic acid
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
EP2087137B1 (en) 2006-10-18 2011-11-30 Epigenomics AG A molecule for providing a standard for the quantitative analysis of the methylation status of a nucleic acid
US20100203514A1 (en) 2006-11-24 2010-08-12 Sledziewski Andrew Z Methods and nucleic acids for the analysis of gene expression associated with the development of prostate cell proliferative disorders
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法
EP2115165B1 (en) 2007-01-19 2012-03-14 Epigenomics AG Methods and nucleic acids for analyses of cell proliferative disorders
US20090203011A1 (en) * 2007-01-19 2009-08-13 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
WO2008091913A2 (en) 2007-01-23 2008-07-31 Sequenom, Inc. Method for accurate assessment of dna quality after bisulfite treatment
WO2008096146A1 (en) * 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
JP2010521142A (ja) * 2007-03-16 2010-06-24 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 遺伝子発現アッセイ
WO2008128198A2 (en) * 2007-04-12 2008-10-23 Usc Stevens - University Of Southern California Dna methylation analysis by digital bisulfite genomic sequencing and digital methylight
MX2009012054A (es) 2007-05-08 2009-11-19 Schering Corp Metodos de tratamiento que usan formulaciones intravenosas que comprenden temozolomida.
WO2009006543A1 (en) * 2007-07-02 2009-01-08 Euclid Diagnostics Llc Methods for evaluating the methylation status of a polynucleotide
US20090042290A1 (en) * 2007-08-06 2009-02-12 Christopher Steele Method of modifying a macromolecule without prior extraction from a sample
EA017879B1 (ru) * 2007-09-17 2013-03-29 Онкометилом Сайенсиз Са Улучшенное определение экспрессии mage-a
US20090246771A1 (en) * 2007-11-02 2009-10-01 University Of Southern California Compositions and methods comprising biomarkers of sperm quality, semen quality and fertility
EP2215250B1 (en) * 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009074328A2 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of lung carcinoma
US20110003700A1 (en) * 2007-12-20 2011-01-06 Human Genetic Signatures Pty Ltd. Elimination of contaminants associated with nucleic acid amplification
DE102008008313A1 (de) 2008-02-07 2009-08-13 Qiagen Gmbh Amplifikation von bisulfitierten Nukleinsäuren
EP2294216A4 (en) 2008-05-14 2011-11-23 Dermtech Int DIAGNOSIS OF MELANOMA AND SOLAR LENTIGO BY ANALYSIS OF NUCLEIC ACIDS
EP2304057B1 (en) 2008-06-17 2014-09-17 Signature Diagnostics AG Method for the detection of ovarian cancer
EP2143807A1 (en) * 2008-06-27 2010-01-13 Epigenomics AG Method for methylation-selective amplification
WO2010003153A2 (en) * 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
US8361746B2 (en) 2008-07-24 2013-01-29 Brookhaven Science Associates, Llc Methods for detection of methyl-CpG dinucleotides
US8541207B2 (en) 2008-10-22 2013-09-24 Illumina, Inc. Preservation of information related to genomic DNA methylation
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
US20100136559A1 (en) * 2008-12-02 2010-06-03 Bio-Rad Laboratories, Inc. Chromatin structure detection
US8497066B2 (en) * 2008-12-04 2013-07-30 Rush University Medical Center DNA methylation based test for monitoring efficacy of treatment
WO2010083046A2 (en) * 2009-01-15 2010-07-22 The Salk Institute For Biological Studies Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
EP2966181B1 (en) 2009-06-26 2018-02-14 Epigenomics AG Methods and nucleic acids for analysis of bladder carcinoma
ES2534200T3 (es) 2009-08-03 2015-04-20 Epigenomics Ag Métodos para la preservación de la complejidad de la secuencia del ADN genómico
EP2287335A1 (en) * 2009-08-05 2011-02-23 DiaSorin S.p.A. Highly sensitive rapid isothermal method for the detection of methylation patterns
WO2011028533A2 (en) * 2009-08-24 2011-03-10 Perkinelmer Health Sciences, Inc. Methods for detecting dna methylation using encoded particles
CA2777906A1 (en) 2009-10-28 2011-05-05 Signature Diagnostics Ag Method for the prognosis of ovarian carcinoma
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
US8568984B2 (en) * 2010-02-19 2013-10-29 Philadelphia Health & Education Corporation Methods of diagnosing non-urinary tract diseases by detecting aberrant methylation
EP2564200B8 (en) 2010-04-27 2019-10-02 The Regents of The University of California Cancer biomarkers and methods of use thereof
DK2576832T3 (en) * 2010-05-25 2016-06-20 Univ Johns Hopkins CONFIGURATIONS AND METHODS FOR DETECTION OF neoplasia
JP5871933B2 (ja) 2010-09-10 2016-03-01 バイオ−ラッド ラボラトリーズ インコーポレーティッド Ｄｎａ内のｒｎａ相互作用領域の検出
CN103314114B (zh) 2010-09-13 2016-08-10 临床基因组学股份有限公司 结直肠癌的外遗传标记以及使用它们的诊断方法
US10428390B2 (en) * 2010-12-16 2019-10-01 Genomictree, Inc. Method for detecting methylation of colorectal cancer specific methylation marker gene for colorectal cancer diagnosis
EP2673380B1 (en) 2011-02-09 2018-12-12 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
EP2675913B1 (en) 2011-02-15 2016-12-14 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
US10435743B2 (en) 2011-05-20 2019-10-08 The Regents Of The University Of California Method to estimate age of individual based on epigenetic markers in biological sample
NO2729579T3 (el) 2011-07-08 2018-03-03
US8728987B2 (en) 2011-08-03 2014-05-20 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
JP6085603B2 (ja) 2011-08-25 2017-02-22 クリニカル ゲノミクス プロプライエタリー リミテッド 結腸直腸がん及び乳がん診断法におけるｄｎａメチル化
WO2013033380A1 (en) 2011-08-31 2013-03-07 Genentech, Inc. Diagnostic markers
CA2848144C (en) 2011-09-07 2018-03-06 Human Genetic Signatures Pty Ltd Molecular detection assay
US20130084287A1 (en) 2011-09-30 2013-04-04 Genentech, Inc. Diagnostic markers
US10131953B2 (en) 2011-11-03 2018-11-20 Wisconsin Alumni Research Foundation Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
CA2858144C (en) 2011-12-06 2021-05-04 Mdxhealth Sa Methods of detecting mutations and epigenetic changes
WO2013096661A1 (en) 2011-12-22 2013-06-27 Illumina, Inc. Methylation biomarkers for ovarian cancer
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
US11254985B2 (en) 2012-05-11 2022-02-22 Clinical Genomics Pty. Ltd. Diagnostic gene marker panel for colorectal cancer
WO2013170191A1 (en) 2012-05-11 2013-11-14 Genentech, Inc. Methods of using antagonists of nad biosynthesis from nicotinamide
ES2778028T3 (es) 2012-05-18 2020-08-07 Clinical Genomics Pty Ltd Un método de cribado para cáncer colorrectal
CA2874407A1 (en) 2012-05-24 2013-11-28 Fundacio Institut D'investigacio Biomedica De Bellvitge (Idibell) Method for the identification of the origin of a cancer of unknown primary origin by methylation analysis
US20150337386A1 (en) * 2012-08-28 2015-11-26 Istat Biomedical Co., Ltd. Test composition for screening cancers
AU2013337353B2 (en) 2012-11-02 2019-04-04 The Johns Hopkins University DNA methylation biomarkers of post-partum depression risk
US9427737B2 (en) 2012-12-14 2016-08-30 Bio-Rad Laboratories, Inc. Methods and compositions for using oils for analysis and detection of molecules
US20140242583A1 (en) * 2013-02-28 2014-08-28 Jim Z. Lu Assays, methods and compositions for diagnosing cancer
CN105378107A (zh) * 2013-03-14 2016-03-02 雅培分子公司 多重甲基化-特异性扩增系统和方法
US20140274757A1 (en) 2013-03-14 2014-09-18 Marie K. Kirby Differential Methylation Level of CpG Loci That Are Determinative of a Biochemical Reoccurrence of Prostate Cancer
CA2905949A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
EP2978861B1 (en) 2013-03-28 2020-07-15 Wisconsin Alumni Research Foundation Unbiased dna methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
WO2014172288A2 (en) 2013-04-19 2014-10-23 Raindance Technologies, Inc. Digital analyte analysis
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
EP3019630B1 (en) 2013-07-11 2018-09-19 The Johns Hopkins University A dna methylation and genotype specific biomarker of suicide attempt and/or suicide ideation
GB201402644D0 (en) * 2014-02-14 2014-04-02 Base4 Innovation Ltd Methylation detection method
MY193347A (en) 2014-05-02 2022-10-06 Malaysian Palm Oil Board Mantle phenotype detection in palm
RU2016147914A (ru) 2014-05-09 2018-06-18 Лайфкодекс Аг Обнаружение днк, которая происходит из специфического клеточного типа, и относящиеся к нему способы
EP2942401A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Detection of DNA that originates from a specific cell-type
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
WO2015181804A2 (en) * 2014-05-30 2015-12-03 Pontificia Universidad Católica De Chile Method and assay for the non-invasive detection of early gastric cancer by the combined use of methylated reprimo cell-free dna and pepsinogen i/ii
MX2016016062A (es) * 2014-06-05 2017-03-16 Clinical Genomics Pty Ltd Metodo para analisis de metilacion.
US11072828B2 (en) 2014-10-06 2021-07-27 The Johns Hopkins University DNA methylation and genotype specific biomarker for predicting post-traumatic stress disorder
WO2016061465A1 (en) * 2014-10-17 2016-04-21 The Regents Of The University Of Colorado, A Body Corporate Biomarkers for head and neck cancer and methods of their use
EP3227687A4 (en) 2014-12-05 2018-10-24 Prelude, Inc. Dcis recurrence and invasive breast cancer
US9984201B2 (en) 2015-01-18 2018-05-29 Youhealth Biotech, Limited Method and system for determining cancer status
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
CN109152824B (zh) 2015-11-27 2022-12-06 卡瑟里克斯私人有限公司 经遗传修饰的细胞及其用途
CA3168463A1 (en) 2015-12-17 2017-06-22 Illumina, Inc. Distinguishing methylation levels in complex biological samples
WO2017136482A1 (en) 2016-02-01 2017-08-10 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
US10202650B2 (en) 2016-05-31 2019-02-12 Youhealth Biotech, Limited Methods for monitoring ELOVL2, KLF14 and PENK gene expression following treatment with vitamin C
US10093986B2 (en) 2016-07-06 2018-10-09 Youhealth Biotech, Limited Leukemia methylation markers and uses thereof
EP3481941A4 (en) 2016-07-06 2020-04-15 Youhealth Biotech, Limited BREAST AND OVARY CANCER METHYLATION MARKERS AND USES THEREOF
CN107847515B (zh) 2016-07-06 2021-01-29 优美佳生物技术有限公司 实体瘤甲基化标志物及其用途
US11130998B2 (en) 2016-11-14 2021-09-28 Wisconsin Alumni Research Foundation Unbiased DNA methylation markers define an extensive field defect in histologically normal prostate tissues associated with prostate cancer: new biomarkers for men with prostate cancer
EP3336197B1 (en) 2016-12-16 2022-04-06 Eurofins Genomics Europe Sequencing GmbH Epigenetic markers and related methods and means for the detection and management of ovarian cancer
EP3589371A4 (en) 2017-03-02 2020-11-25 Youhealth Oncotech, Limited METHYLATION MARKERS FOR THE DIAGNOSIS OF HEPER CELL CARCINOMA AND LUNG CANCER
EP3655418A4 (en) 2017-06-22 2021-05-19 Triact Therapeutics, Inc. METHOD OF TREATMENT OF GLIOBLASTOMA
EP3687501A4 (en) 2017-09-29 2021-06-23 Triact Therapeutics, Inc. INIPARIB FORMS AND USES THEREOF
WO2020056338A1 (en) 2018-09-14 2020-03-19 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
SG11202107670TA (en) 2019-01-18 2021-08-30 Univ California Dna methylation measurement for mammals based on conserved loci
EP3948290A4 (en) 2019-03-26 2023-08-09 Dermtech, Inc. NEW GENE CLASSIFIERS AND THEIR USES FOR SKIN CANCERS
US11542548B2 (en) 2019-06-11 2023-01-03 Wisconsin Alumni Research Foundation; Blood DNA methylation biomarker diagnostic test for anxiety and depressive disorders
CN112662761A (zh) * 2020-03-05 2021-04-16 博尔诚（北京）科技有限公司 一种检测3种实质性器官肿瘤的探针组合物
WO2021237103A1 (en) 2020-05-22 2021-11-25 Wisconsin Alumni Research Foundation Epigenetic modification of select genes as markers of hypersomnolence
WO2023052640A1 (en) 2021-09-30 2023-04-06 Tivenix Sa A method for diagnosing and predicting progression of neurodegenerative diseases or disorders
EP4170661A1 (en) 2021-10-19 2023-04-26 Institut National de la Santé et de la Recherche Médicale (INSERM) Methylation profile analysis using smoothing method
WO2023066972A1 (en) 2021-10-19 2023-04-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Dna methylation signature for diagnosing hepatocellular carcinoma
WO2023175019A1 (en) 2022-03-15 2023-09-21 Genknowme S.A. Method determining the difference between the biological age and the chronological age of a subject
WO2024044578A1 (en) 2022-08-22 2024-02-29 University Of Virginia Dna methylation biomarkers of premenstrual dysphoric disorder and perimenopausal depression
WO2024059649A1 (en) 2022-09-13 2024-03-21 The Saban Research Institute; Children's Hospital Los Angeles Dna methylation signature of retinoblastoma

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5788146A (en) * 1996-02-13 1998-08-04 Bradford Company Parent welding partition assembly
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
EP1179600B1 (en) 1996-06-04 2005-05-11 University Of Utah Research Foundation Monitoring hybridization during PCR
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
AUPP312998A0 (en) 1998-04-23 1998-05-14 Commonwealth Scientific And Industrial Research Organisation Diagnostic assay
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6472156B1 (en) * 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
CA2487578A1 (en) 2003-12-11 2005-06-11 Epigenomics Ag Prognostic markers for prediction of treatment response and/or survival of breast cell proliferative disorder patients
US20050287553A1 (en) 2004-04-06 2005-12-29 Epigenomics Ag Method for the quantification of methylated DNA
EP2311984A1 (en) 2004-12-02 2011-04-20 Epigenomics AG Methods and nucleic acids for the analysis of gene expression associated with the prognosis of prostate cell proliferative disorders

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