BR0110671A - Método para detecção de genomas minoritários em quase-espécies virais e kit para detecção de genomas minoritários em quase-espécies virais - Google Patents
Método para detecção de genomas minoritários em quase-espécies virais e kit para detecção de genomas minoritários em quase-espécies viraisInfo
- Publication number
- BR0110671A BR0110671A BR0110671-6A BR0110671A BR0110671A BR 0110671 A BR0110671 A BR 0110671A BR 0110671 A BR0110671 A BR 0110671A BR 0110671 A BR0110671 A BR 0110671A
- Authority
- BR
- Brazil
- Prior art keywords
- species
- quasi
- viral
- genomes
- minority genomes
- Prior art date
Links
- 230000003612 virological effect Effects 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 238000001514 detection method Methods 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 108091027305 Heteroduplex Proteins 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 238000010369 molecular cloning Methods 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
- C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/706—Specific hybridization probes for hepatitis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- AIDS & HIV (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
"MéTODO PARA DETECçãO DE GENOMAS MINORITáRIOS EM QUASE-ESPéCIES VIRAIS E KIT PARA DETECçãO DE GENOMAS MINORITáRIOS EM QUASE-ESPéCIES VIRAIS". Sendo que método permite a detecção de genomas minoritários, em particular, genomas memória minoritários, de uma população de ácidos nucléicos de uma quase-espécie viral, presentes em uma proporção inferior a 50%, que contêm, pelo menos, uma mutação quanto ao genoma majoritário da referida quase-espécie, e compreende: a) extrair o ácido nucléico da referida quase-espécie viral a partir de uma amostra suspeita de conter a referida quase-espécie viral; b) amplificar, pelo menos, um fragmento do ácido nucléico da referida quase-espécie viral; e c) detectar e analisar a existência de genomas minoritários, mediante a utilização de técnicas baseadas no uso de microchips de DNA, o ensaio da impressão do heteroduplex (HTA ) e a clonagem molecular. O método tem aplicação no diagnóstico genético de doenças virais.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200001068 | 2000-04-27 | ||
PCT/ES2001/000165 WO2001083815A1 (es) | 2000-04-27 | 2001-04-27 | Método para la deteccion de genomas minoritarios en cuasiespecies virales mediante el empleo de microchips de dna |
Publications (1)
Publication Number | Publication Date |
---|---|
BR0110671A true BR0110671A (pt) | 2003-12-30 |
Family
ID=8493311
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR0110671-6A BR0110671A (pt) | 2000-04-27 | 2001-04-27 | Método para detecção de genomas minoritários em quase-espécies virais e kit para detecção de genomas minoritários em quase-espécies virais |
Country Status (12)
Country | Link |
---|---|
US (1) | US20030180717A1 (pt) |
EP (1) | EP1284296B1 (pt) |
AT (1) | ATE342379T1 (pt) |
AU (1) | AU5636201A (pt) |
BR (1) | BR0110671A (pt) |
CA (1) | CA2407753A1 (pt) |
CY (1) | CY1105861T1 (pt) |
DE (1) | DE60123781T2 (pt) |
DK (1) | DK1284296T3 (pt) |
ES (1) | ES2273835T3 (pt) |
PT (1) | PT1284296E (pt) |
WO (1) | WO2001083815A1 (pt) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7718356B2 (en) * | 2000-09-26 | 2010-05-18 | Health Research Inc. | Heteroduplex tracking assay |
US7344830B2 (en) * | 2000-09-26 | 2008-03-18 | Health Research Inc. | Heteroduplex tracking assay |
WO2002027321A2 (en) * | 2000-09-26 | 2002-04-04 | Health Research Incorporated | Analysis of hiv-1 coreceptor use in the clinical care of hiv-1-infected patients |
CN1982473B (zh) * | 2005-12-16 | 2010-05-05 | 天津生物芯片技术有限责任公司 | 检测hiv对逆转录酶抑制剂抗性的基因芯片及试剂盒 |
EP2025762A3 (en) * | 2006-01-17 | 2009-09-30 | Health Research Inc. | Heteroduplex tracking assay |
KR102218512B1 (ko) * | 2010-05-25 | 2021-02-19 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | Bambam:고처리율 서열분석 데이터의 병렬 비교 분석 |
US9646134B2 (en) | 2010-05-25 | 2017-05-09 | The Regents Of The University Of California | Bambam: parallel comparative analysis of high-throughput sequencing data |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5861242A (en) * | 1993-06-25 | 1999-01-19 | Affymetrix, Inc. | Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same |
US5871697A (en) * | 1995-10-24 | 1999-02-16 | Curagen Corporation | Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
WO1997039375A1 (de) * | 1996-04-16 | 1997-10-23 | Leica Lasertechnik Gmbh | Punktlichtquelle für ein laserscanmikroskop und verfahren zum einkoppeln von mindestens zwei lasern unterschiedlicher wellenlänge in ein laserscanmikroskop |
US6143496A (en) * | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
US6759193B2 (en) * | 1999-07-28 | 2004-07-06 | The Government Of The Republic Of Singapore | Detection of human hepatitis B virus surface antigen mutants by specific amplification and its application on gene chip |
-
2001
- 2001-04-27 PT PT01929654T patent/PT1284296E/pt unknown
- 2001-04-27 BR BR0110671-6A patent/BR0110671A/pt not_active IP Right Cessation
- 2001-04-27 CA CA002407753A patent/CA2407753A1/en not_active Abandoned
- 2001-04-27 DK DK01929654T patent/DK1284296T3/da active
- 2001-04-27 WO PCT/ES2001/000165 patent/WO2001083815A1/es active IP Right Grant
- 2001-04-27 US US10/258,765 patent/US20030180717A1/en not_active Abandoned
- 2001-04-27 EP EP01929654A patent/EP1284296B1/en not_active Expired - Lifetime
- 2001-04-27 DE DE60123781T patent/DE60123781T2/de not_active Expired - Fee Related
- 2001-04-27 AU AU56362/01A patent/AU5636201A/en not_active Abandoned
- 2001-04-27 AT AT01929654T patent/ATE342379T1/de not_active IP Right Cessation
- 2001-04-27 ES ES01929654T patent/ES2273835T3/es not_active Expired - Lifetime
-
2006
- 2006-12-19 CY CY20061101813T patent/CY1105861T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
CA2407753A1 (en) | 2001-11-08 |
PT1284296E (pt) | 2007-01-31 |
ES2273835T3 (es) | 2007-05-16 |
DK1284296T3 (da) | 2007-02-05 |
AU5636201A (en) | 2001-11-12 |
US20030180717A1 (en) | 2003-09-25 |
DE60123781D1 (de) | 2006-11-23 |
DE60123781T2 (de) | 2007-08-16 |
EP1284296B1 (en) | 2006-10-11 |
EP1284296A1 (en) | 2003-02-19 |
CY1105861T1 (el) | 2011-02-02 |
ATE342379T1 (de) | 2006-11-15 |
WO2001083815A1 (es) | 2001-11-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 9A E 10A ANUIDADE(S). |
|
B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2144 DE 07/02/2012. |