CN101652474B - 由地衣芽孢杆菌转化细胞制备脂酰基转移酶 - Google Patents

由地衣芽孢杆菌转化细胞制备脂酰基转移酶 Download PDF

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Publication number
CN101652474B
CN101652474B CN200780050429XA CN200780050429A CN101652474B CN 101652474 B CN101652474 B CN 101652474B CN 200780050429X A CN200780050429X A CN 200780050429XA CN 200780050429 A CN200780050429 A CN 200780050429A CN 101652474 B CN101652474 B CN 101652474B
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seq
nucleotide sequence
sequence
amino acid
acyltransferase
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CN101652474A (zh
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马克·科尔克曼
约恩·达尔高·米克尔森
里克·赫格·洛伦森
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Danisco US Inc
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Danisco AS
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200780050429XA 2007-01-25 2007-01-25 由地衣芽孢杆菌转化细胞制备脂酰基转移酶 Expired - Fee Related CN101652474B (zh)

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Application Number Priority Date Filing Date Title
PCT/IB2007/000558 WO2008090395A1 (en) 2007-01-25 2007-01-25 Production of a lipid acyltransferase from transformed bacillus licheniformis cells

Related Child Applications (1)

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CN201210031434.9A Division CN102559627B (zh) 2007-01-25 2007-01-25 由地衣芽孢杆菌转化细胞制备脂酰基转移酶

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CN101652474A CN101652474A (zh) 2010-02-17
CN101652474B true CN101652474B (zh) 2012-06-27

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US (1) US7960150B2 (enExample)
EP (2) EP2109670A1 (enExample)
JP (1) JP5124593B2 (enExample)
CN (1) CN101652474B (enExample)
AU (1) AU2007344910B2 (enExample)
BR (1) BRPI0720801A2 (enExample)
CA (1) CA2673954C (enExample)
DK (1) DK2405007T5 (enExample)
MX (1) MX2009008021A (enExample)
PL (1) PL2405007T3 (enExample)
WO (1) WO2008090395A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE362315T1 (de) 2001-05-18 2007-06-15 Danisco Verfahren zur herstellung von teig mit einem enzym
GB0716126D0 (en) * 2007-08-17 2007-09-26 Danisco Process
WO2006008508A1 (en) 2004-07-16 2006-01-26 Danisco A/S Enzymatic oil-degumming method
EA020035B1 (ru) 2007-12-21 2014-08-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы
CN101418276B (zh) * 2008-12-08 2010-12-22 江南大学 一种宿主细胞及其用于重组蛋白高效分泌表达的方法
GB0905367D0 (en) * 2009-03-27 2009-05-13 Danisco Method
CN102656264A (zh) * 2009-10-15 2012-09-05 丹尼斯科公司 脂质酰基转移酶蛋白及其制备方法
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
GB201007668D0 (en) 2010-05-07 2010-06-23 Danisco Method
PH12021552871A1 (en) * 2011-12-27 2022-09-28 Commw Scient Ind Res Org Processes for producing lipids
JP5926801B2 (ja) * 2012-07-17 2016-05-25 国立大学法人福島大学 プラスマローゲンの加水分解方法、プラスマローゲンの測定方法
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
KR101994252B1 (ko) * 2016-06-30 2019-06-28 명지대학교 산학협력단 베타 아가레이즈의 생산능을 갖는 재조합 바실러스 서브틸리스 균주 및 이의 용도
CN110592100B (zh) * 2019-10-08 2022-08-02 海南大学 一种木薯camta基因及其抑制表达载体的构建和抗病应用
CN119899821B (zh) * 2024-07-01 2025-10-17 华南理工大学 一种紫红链霉菌磷脂酶a2突变体及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2403025A1 (en) * 2002-10-08 2004-04-08 Cognis Deutschland Gmbh & Co. Kg Enzymes with lipase/acyltransferase activity
WO2004064987A2 (en) * 2003-01-17 2004-08-05 Danisco A/S Method
CN1898386A (zh) * 2003-12-24 2007-01-17 丹尼斯科公司 蛋白质

Family Cites Families (248)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT110768B (de) 1927-09-29 1928-10-10 Patiag Patentverwertungs Und I Windkraftmaschine.
US2888385A (en) * 1952-11-28 1959-05-26 Grandel Felix Process of making a preparation containing lipases and oxidases
US3260606A (en) * 1964-04-29 1966-07-12 Taiyo Food Co Ltd Enzymatic treatment of egg
GB1092775A (en) * 1965-07-07 1967-11-29 Knud Aunstrup Preparation of amyloglucosidase
US3368903A (en) 1966-02-18 1968-02-13 Vanderbilt Co R T Baked goods dough and method
CH461935A (fr) * 1966-05-03 1968-08-31 Menzi Robert Procédé de fabrication de pâtes alimentaires séchées
DE1900959A1 (de) * 1969-01-09 1970-08-27 Unilever Nv Verfahren zur Herstellung von Pflanzenphosphatiden mit universeller Emulgierkraft
US3634195A (en) * 1969-09-08 1972-01-11 Miles Lab Production of lipase
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
GB1375783A (enExample) 1972-02-04 1974-11-27
GB1442418A (en) 1972-12-14 1976-07-14 Procter & Gamble Method of cleansing polyester-containing fabrics
IL46862A (en) 1974-04-08 1977-12-30 Baxter Travenol Lab Lipolytic enzyme flavoring system for fat-containing food
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3973042A (en) 1974-05-10 1976-08-03 Cornell Research Foundation, Inc. Flavor development by microbial lipases in pasteurized milk blue cheese
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
GB1525929A (en) * 1974-11-25 1978-09-27 Unilever Ltd Stabilised emulsions comprising phospholipoprotein
GB1577933A (en) 1976-02-11 1980-10-29 Unilever Ltd Fat process and composition
US4160848A (en) * 1977-04-18 1979-07-10 Pennwalt Corporation Antistaling agent for bakery products
JPS6049477B2 (ja) 1977-04-19 1985-11-01 協和醗酵工業株式会社 グリセロ−ル酸化酵素およびその製造法ならびにグリセロ−ル酸化酵素を用いるグリセロ−ルの定量法
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
JPS5850719B2 (ja) 1978-10-18 1983-11-11 協和醗酵工業株式会社 トリグリセライドの定量方法
US4399218A (en) 1980-02-05 1983-08-16 Boehringer Mannheim Gmbh Method and reagent for the determination of glycerin
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
JPS58500638A (ja) 1981-05-07 1983-04-28 ユニリ−バ− ナ−ムロ−ゼ ベンノ−トシヤ−プ エステルの合成法
JPS6030488B2 (ja) * 1982-11-10 1985-07-17 協和醗酵工業株式会社 生地の改良剤およびそれを含有してなる生地
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DK289083A (da) 1983-06-23 1984-12-24 Novo Industri As Lipase, fremgangsmaade til fremstilling deraf og anvendelse deraf
DK402583D0 (da) * 1983-09-05 1983-09-05 Novo Industri As Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf
DK152763C (da) 1983-09-05 1988-10-24 Novo Industri As Fremgangsmaade til fremstilling af et immobiliseret lipasepraeparat
US4707364A (en) 1984-01-27 1987-11-17 Miles Laboratories, Inc. Composition for accelerating cheese aging
US4636468A (en) 1984-06-25 1987-01-13 Genencor, Inc. Lipolytic enzyme derived from a aspergillus microorganism having an accelerating effect on cheese flavor development
CA1262654A (en) 1984-08-10 1989-11-07 Takaoki Torigoe Food quality improving agent
NL8402979A (nl) * 1984-09-28 1986-04-16 Tno Werkwijze voor het bestrijden van vaatverwelkingsziekten bij gewassen, in het bijzonder de iepeziekte.
JPS61181390A (ja) 1985-02-06 1986-08-14 Amano Pharmaceut Co Ltd 酵素によるグリセライドの製造法
US4689297A (en) 1985-03-05 1987-08-25 Miles Laboratories, Inc. Dust free particulate enzyme formulation
EP0195311B2 (en) 1985-03-06 1996-01-17 Yoshikawa Oil & Fat Co., Ltd. Process for preparing fatty acid esters
US5219733A (en) * 1985-03-06 1993-06-15 Yoshikawa Oil & Fat Co., Ltd. Process for preparing fatty acid esters
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPS61242542A (ja) 1985-04-22 1986-10-28 Fuji Oil Co Ltd チ−ズ風味材の製造法
US5310679A (en) * 1985-05-13 1994-05-10 Artiss Joseph D Composition for reducing turbidity in samples of biological fluids
GB8514708D0 (en) 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
GB8514707D0 (en) 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
GB8525012D0 (en) * 1985-10-10 1985-11-13 Cpc International Inc Carbohydrate refining process
GB2185990B (en) 1986-02-05 1990-01-24 Unilever Plc Margarine fat
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5874558A (en) * 1986-03-17 1999-02-23 Novo Nordisk Nucleic acid encoding a recombinant humicola sp. lipase
JPS62262997A (ja) * 1986-05-02 1987-11-16 ノボ ノルディスク アクティーゼルスカブ ワツクスの製造方法
JPS6344892A (ja) 1986-08-13 1988-02-25 Kao Corp 油脂類のエステル交換反応方法
DK157560C (da) 1986-08-29 1990-06-11 Novo Nordisk As Enzymatisk detergentadditiv, detergent indeholdende det enzymatiske detergentadditiv og fremgangsmaade til vask af tekstiler.
ES2058119T3 (es) * 1986-08-29 1994-11-01 Novo Nordisk As Aditivo detergente enzimatico.
EP0260573A3 (de) 1986-09-18 1989-03-22 Lucas Meyer GmbH & Co Verfahren zur Herstellung eines hydrolysierten Lecithins, sowie Anwendung des hydrolysierten Lecithins
US5273898A (en) 1986-10-17 1993-12-28 Noro Nordisk A/S Thermally stable and positionally non-specific lipase isolated from Candida
US5108457A (en) 1986-11-19 1992-04-28 The Clorox Company Enzymatic peracid bleaching system with modified enzyme
KR900003014B1 (ko) * 1986-12-27 1990-05-04 도오아 야꾸힌 고오교오 가부시끼가이샤 양식어(養殖魚)용 사료 첨가제
US5219744A (en) * 1987-08-26 1993-06-15 Ajinomoto Co., Inc. Process for modifying fats and oils
ES2076939T3 (es) 1987-08-28 1995-11-16 Novo Nordisk As Lipasa recombinante de humicola y procedimiento para la produccion de lipasas recombinantes de humicola.
EP0321811B2 (en) 1987-12-21 1999-12-22 Dsm N.V. Method for improving flour dough
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
DK77788A (da) * 1988-02-16 1989-08-17 Novo Industri As Fremgangsmaade til fremstilling af kokosnoedolie
JP3079276B2 (ja) * 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
EP0334462B2 (en) 1988-03-25 2002-04-24 Genencor International, Inc. Molecular cloning and expression of genes encoding lipolytic enzymes
US5232846A (en) 1988-08-09 1993-08-03 Unitika Ltd. Method for producing a thermostable lipoprotein lipase from streptomyces
GB8906837D0 (en) 1989-03-23 1989-05-10 Unilever Plc Bread improvers
DE3920561A1 (de) * 1989-06-23 1991-01-10 Knoll Ag Verfahren zur vermeidung von verdauungsstoerungen bei pflanzenfressenden tieren
US5213968A (en) * 1989-08-21 1993-05-25 Nestec S.A. Process for preparing emulsifying agents
DE69004782T2 (de) 1989-09-29 1994-03-17 Unilever Nv Getrocknetes Lyso-Phospholipoprotein enthaltendes Nahrungsmittel.
US5677160A (en) 1989-10-30 1997-10-14 Henkel Corporation Fat splitting process
US5288619A (en) * 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
JPH03262492A (ja) 1990-03-06 1991-11-22 P Macneil Gerald モノグリセリドの製造方法
DK19991D0 (da) * 1991-02-06 1991-02-06 Novo Nordisk As Proteinpraeparationer
KR100225087B1 (ko) 1990-03-23 1999-10-15 한스 발터라벤 피타아제의 식물내 발현
JP3110452B2 (ja) 1990-05-09 2000-11-20 ノボ ノルディスク アクティーゼルスカブ エンドグルカナーゼ酵素を含んでなるセルラーゼ調製物
US5814501A (en) 1990-06-04 1998-09-29 Genencor International, Inc. Process for making dust-free enzyme-containing particles from an enzyme-containing fermentation broth
EP0468731A1 (en) 1990-07-26 1992-01-29 Oriental Yeast Co., Ltd. Bread improver and method of producing bread
US5869438A (en) * 1990-09-13 1999-02-09 Novo Nordisk A/S Lipase variants
CA2092615A1 (en) 1990-09-13 1992-03-14 Allan Svendsen Lipase variants
US5892013A (en) * 1990-09-13 1999-04-06 Novo Nordisk A/S Lipase variants
CA2058056C (en) 1990-12-21 1996-11-05 Chiaki Saito Method of decreasing cholesterol concentration in food
PH31068A (en) 1991-03-07 1998-02-05 Ici Plc Process for the production of terephthalic acid.
DE4112440C1 (enExample) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
EP0585285B1 (en) * 1991-05-01 1998-08-12 Novo Nordisk A/S Stabilized enzymes
DE4115938A1 (de) 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
CA2077020A1 (en) 1991-09-03 1993-03-04 Yoshikazu Isono Process for producing lipoprotein-containing substance having reduced lipid content and food containing substance thus produced
US5879920A (en) * 1991-10-07 1999-03-09 Genencor International, Inc. Coated enzyme-containing granule
ATE133196T1 (de) 1991-11-11 1996-02-15 Akzo Nobel Nv Verfahren zur herstellung von salzgranulaten
EP0558112A1 (en) 1992-02-25 1993-09-01 Unilever N.V. Enzymic diglyceride removal
CA2079839A1 (en) 1992-02-28 1993-08-29 Vincent Destefanis Calcium peroxide and ascorbic acid containing compositions as replacements for bromate in breadmaking
GB2267033B (en) 1992-03-07 1996-01-24 David Garnett Lysophospholipid Animal Feed Supplement
DK42092D0 (enExample) 1992-03-27 1992-03-27 Novo Nordisk As
DK73592D0 (da) * 1992-06-03 1992-06-03 Novo Nordisk As Nyt enzym
ATE222599T1 (de) * 1992-06-16 2002-09-15 Sankyo Co Phospholipase a1, verfahren zu seiner herstellung und anwendung
DK88892D0 (da) * 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
EP0580252A2 (en) 1992-07-20 1994-01-26 Quest International B.V. Improvements in or relating to pectin methyl esterase
ATE135163T1 (de) 1992-07-27 1996-03-15 Gist Brocades Nv Enzymprodukt und verfahren zur verbesserung der qualität von brot
DK104592D0 (da) 1992-08-21 1992-08-21 Novo Nordisk As Fremgangsmaade
JPH08504327A (ja) 1992-12-10 1996-05-14 ギスト ブロカデス ナムローゼ フェンノートシャップ 糸状菌における異種タンパクの生産
JP3507076B2 (ja) * 1992-12-22 2004-03-15 ノボザイムス アクティーゼルスカブ アルカリリパーゼ
DK154292D0 (da) 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
JP2937746B2 (ja) 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法
ZA943640B (en) 1993-06-07 1995-01-26 Buckman Labor Inc Synergistically stabilized liquid enzymatic compositions
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
JP2678341B2 (ja) 1993-09-27 1997-11-17 富士紡績株式会社 固定化リパーゼ
EP0650669B1 (en) 1993-10-29 2002-01-09 Dsm N.V. Baking improver compositions
EP0652289A1 (en) 1993-11-05 1995-05-10 Unilever Plc Random interesterification of triglyceride fats
DE4339556C1 (de) 1993-11-19 1995-02-02 Metallgesellschaft Ag Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
EP1090553A3 (en) * 1993-12-24 2001-04-18 Dsm N.V. Dry yeast compositions
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1995022606A1 (en) 1994-02-21 1995-08-24 Novo Nordisk A/S Method for production of an immobilized enzyme preparation and use of the immobilized enzyme preparation
ATE222604T1 (de) 1994-02-22 2002-09-15 Novozymes As Methode zur herstellung einer variante eines lipolytischen enzymes
US5834280A (en) 1994-05-03 1998-11-10 Novo Nordisk A/S Glucose oxidases obtained from a cladosporium
US5741665A (en) * 1994-05-10 1998-04-21 University Of Hawaii Light-regulated promoters for production of heterologous proteins in filamentous fungi
DE69501228T2 (de) 1994-05-11 1998-05-07 Amano Pharma Co Ltd Ascorbatoxidase, dafür kodierendes Gen, Verfahren zu ihrer Herstellung und dieselbe verwendende Reagens-Zusammensetzung
WO1995034222A1 (fr) 1994-06-16 1995-12-21 Firmenich S.A. Procede d'aromatisation et composition aromatisante
DE69519331D1 (de) 1994-06-17 2000-12-14 Dsm Nv Zusammensetzung zur Brotverbesserung
CN1167503A (zh) 1994-10-26 1997-12-10 诺沃挪第克公司 一种具有脂解活性的酶
EP0784675A1 (en) * 1994-10-26 1997-07-23 Novo Nordisk A/S Enzymatic detergent composition
ES2181792T3 (es) 1994-10-26 2003-03-01 Novozymes As Nueva enzima lipolitica.
GB2296011B (en) 1994-12-13 1999-06-16 Solvay Novel fusarium isolate and lipases, cutinases and enzyme compositions derived therefrom
US6093562A (en) * 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
JP3394975B2 (ja) 1995-02-22 2003-04-07 セレスター ユーエスエー インク 動物性脂肪からステロールおよび遊離脂肪酸を削減する方法
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
JP3359777B2 (ja) * 1995-03-06 2002-12-24 日清製粉株式会社 油揚げ即席麺およびその製造方法
AU5002096A (en) 1995-03-30 1996-10-16 Novo Nordisk A/S Alkaline lipolytic enzyme
US5919746A (en) * 1995-03-30 1999-07-06 Novo Nordisk A/S Alkaline lipolytic enzyme
US5989599A (en) 1995-04-24 1999-11-23 Nestec S.A. Process for the interesterification of phospholipids
EP0743017B1 (en) 1995-05-15 2004-09-29 DSM IP Assets B.V. Application of phospholipases in animal feed
GB2301103B (en) 1995-05-23 1999-12-22 Danisco An enzyme system comprising ferulic acid esterase
PL184045B1 (pl) * 1995-06-07 2002-08-30 Danisco Sposób polepszania reologicznych właściwości ciasta z mąki oraz jakości wytworzonego z ciasta wyrobu gotowego
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
GB0112226D0 (en) 2001-05-18 2001-07-11 Danisco Method of improving dough and bread quality
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
CN1193346A (zh) 1995-07-14 1998-09-16 诺沃挪第克公司 一种具有脂解活性的修饰酶
DE19527274A1 (de) 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
CN1192780B (zh) 1995-08-11 2010-08-04 诺沃奇梅兹有限公司 新的脂解酶
JP4053595B2 (ja) 1995-09-22 2008-02-27 メディカル・リサーチ・カウンシル 核酸の突然変異誘発におけるまたはそれに関する改良
EP0776604B1 (en) 1995-12-01 2002-01-23 Unilever N.V. Microwavable crispy bread-rolls
US6344328B1 (en) 1995-12-07 2002-02-05 Diversa Corporation Method for screening for enzyme activity
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5756328A (en) * 1995-12-20 1998-05-26 Cornell Research Foundation, Inc. Acyltransferase and cDNA encoding acyltransferase
US5942430A (en) 1996-02-16 1999-08-24 Diversa Corporation Esterases
US6001586A (en) 1996-03-29 1999-12-14 Genencor International, Inc. Compartmentalization method for screening microorganisms
ATE279512T1 (de) 1996-04-25 2004-10-15 Novozymes As Alkalisches, lipolytisches enzym
DE19620649A1 (de) 1996-05-22 1997-11-27 Roehm Gmbh Rekombinant hergestellte Lysophospholipase aus Aspergillus
JPH10155493A (ja) 1996-10-04 1998-06-16 Sankyo Co Ltd アスペルギルス属由来のホスホリパーゼa1をコードする遺伝子
JP3182381B2 (ja) 1996-10-25 2001-07-03 日清製粉株式会社 麺類の機械製麺法
DE19648343C1 (de) * 1996-11-22 1998-02-12 Roehm Gmbh Verfahren zur Herstellung von Backwaren mit verbesserter Frischhaltung
JP3582265B2 (ja) 1996-11-28 2004-10-27 味の素株式会社 改質穀粉及びこれを使用した穀粉加工食品
CN1148442C (zh) 1996-12-09 2004-05-05 诺维信公司 利用来自具有磷脂酶a和/或b活性的丝状真菌的磷脂酶降低含有大量非水合磷的食用油中的含磷成分
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
DE19701348A1 (de) 1997-01-16 1998-07-23 Roehm Gmbh Protein mit Phospholipaseaktivität
US5821102A (en) 1997-01-21 1998-10-13 Novo Nordisk Biotech Inc. Nucleic acids encoding polyeptides having absidia lipase activity
DK0977869T4 (da) 1997-04-09 2009-01-19 Danisco Lipase og brug af samme til forbedring af dej og bageriprodukter
EP0882797B1 (en) 1997-06-04 2003-07-16 Loders Croklaan B.V. Preparation of symmetrical triglycerides aba
RU2140751C1 (ru) 1997-06-11 1999-11-10 Ассоциация "Ассоя" Пищевая добавка для производства хлеба и хлебобулочных изделий
JP4121186B2 (ja) 1997-06-16 2008-07-23 株式会社日立製作所 セラミックス管の検査方法及びその装置
EP0913468A3 (en) 1997-07-22 2001-06-13 Dsm N.V. Bread improving comüposition
EP0897667B2 (en) 1997-07-31 2008-11-12 DSM IP Assets B.V. Bread improving composition
US6355282B1 (en) 1997-10-31 2002-03-12 Amano Pharmaceutical Co., Ltd. Dough composition and preparation thereof
AU1556699A (en) 1997-12-23 1999-07-19 Novo Nordisk A/S A process for immobilisation of enzymes
US6156548A (en) * 1997-12-23 2000-12-05 Novo Nordisk A/S Immobilization of enzymes with a fluidized bed for use in an organic medium
AU3247699A (en) 1998-02-17 1999-09-06 Novo Nordisk A/S Lipase variant
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6815190B1 (en) 1998-04-12 2004-11-09 Novozymes A/S Cutinase variants
WO1999053769A1 (en) 1998-04-20 1999-10-28 Novo Nordisk A/S Preparation of dough and baked products
US6365204B1 (en) * 1998-04-20 2002-04-02 Novozymes Preparation of dough and baked products
US6866837B2 (en) * 1998-06-05 2005-03-15 Mallinckrodt Inc. Radiolabeled peptides for the diagnosis and treatment of breast and prostate tumors and metastases of such tumors
US20030074695A1 (en) * 1998-06-24 2003-04-17 Farese Robert V. Plant diacylglycerol O-acyltransferase and uses thereof
WO2000001713A2 (en) * 1998-07-02 2000-01-13 Calgene Llc Diacylglycerol acyl transferase proteins
SE510497C2 (sv) 1998-07-15 1999-05-31 Biofeed Thailand Co Ltd Funktionellt födoämne innehållande mikroorganismer
ES2188190T5 (es) 1998-07-21 2007-11-16 Danisco A/S Producto alimentario.
JP3414652B2 (ja) 1998-10-12 2003-06-09 敷島製パン株式会社 小麦粉焼成品、その製造方法および品質改良剤
DK1131444T3 (da) * 1998-11-10 2006-08-07 Novozymes Inc Polypeptider med lysophospholipase aktivitet og nucleinsyre som koder for disse
US7312062B2 (en) * 1998-11-27 2007-12-25 Novozymes A/S Lipolytic enzyme variants
RU2235775C2 (ru) 1998-11-27 2004-09-10 Новозимс А/С Способ получения варианта липолитического фермента и липолитический фермент (варианты)
NZ511340A (en) 1998-11-27 2003-07-25 Novozymes As Lipolytic enzyme variants
JP2000226335A (ja) 1998-12-04 2000-08-15 Amano Pharmaceut Co Ltd 経口用酵素製剤、酵素含有食材及び酵素製剤の服用方法
BRPI9915832B1 (pt) 1998-12-04 2016-09-13 Novozymes As "variante de uma cutinase de cepa dsm 1800 de humicola insolens precursora, sequência de dna, vetor, célula hospedeira microbiana transformada, processos para produzir uma variante, para hidrólise enzimática de um oligômero cíclico de poli(etileno tereftalato), para tingir pano ou fio de poliéster, para melhorar o acabamento funcional de um fio ou pano contendo pet, e, composilão detergente".
US6399121B1 (en) 1999-03-16 2002-06-04 Novozymes A/S Process for producing cheese
JP4271872B2 (ja) 1999-03-16 2009-06-03 ノボザイムス アクティーゼルスカブ チーズの製造方法
AU4039400A (en) 1999-03-26 2000-10-16 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
ATE416252T1 (de) 1999-06-02 2008-12-15 Novozymes As Chemisch veränderte, lipolytische enzyme
EP1057415A1 (en) 1999-06-04 2000-12-06 Societe Des Produits Nestle S.A. Lipase-treated pasta and manufacturing process
US6254645B1 (en) * 1999-08-20 2001-07-03 Genencor International, Inc. Enzymatic modification of the surface of a polyester fiber or article
US6337187B1 (en) 1999-11-05 2002-01-08 Millennium Pharmaceuticals, Inc. 18891, a novel human lipase
AU783277B2 (en) * 1999-10-14 2005-10-06 Novozymes A/S Lysophospholipase from aspergillus
US6146869A (en) 1999-10-21 2000-11-14 Novo Nordisk Biotech, Inc. Polypeptides having phospholipase B activity and nucleic acids encoding same
DE19953854C2 (de) 1999-11-09 2002-01-17 Max Planck Gesellschaft Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften
GB2358784B (en) 1999-12-03 2004-06-30 Danisco Method of improving dough and bread quality
EP1233676A1 (en) 1999-12-03 2002-08-28 Danisco A/S Method of improving dough and bread quality
US7078205B2 (en) * 2000-02-17 2006-07-18 Millennium Pharmaceuticals, Inc. Nucleic acid sequences encoding melanoma associated antigen molecules, aminotransferase molecules, atpase molecules, acyltransferase molecules, pyridoxal-phosphate dependent enzyme molecules and uses therefor
DE10119972A1 (de) 2000-04-15 2002-05-23 Nikolaus Weber Enzymatisches Verfahren zur Herstellung von Fettsäuresterylestern aus Dämpferdestillaten der Fettraffination und Tallöl
US20020192792A1 (en) 2000-04-28 2002-12-19 Palle Schneider Laccase mutants
US6509182B2 (en) * 2000-06-26 2003-01-21 Novozymes A/S Lipolytic enzymes
US6432898B1 (en) * 2000-10-31 2002-08-13 Novozymes Biotech, Inc. Polypeptides having lipase activity and nucleic acids encoding same
US6511837B2 (en) * 2000-06-26 2003-01-28 Novozymes A/S Lipolytic enzymes
US6558715B1 (en) * 2000-10-31 2003-05-06 Novozymes Biotech, Inc. Methods for using lipases in baking
US6506588B2 (en) * 2000-06-26 2003-01-14 Novozymes, A/S Lipolytic enzymes
WO2002002762A1 (en) 2000-07-03 2002-01-10 Mochida Pharmaceutical Co., Ltd. Novel lipase
EP1301080B1 (en) * 2000-07-06 2011-09-14 Novozymes A/S Method of preparing a dough, or a baked product made from a dough, with addition of lipolytic enzymes
EP1307548A2 (en) 2000-07-13 2003-05-07 Maxygen, Inc. Novel lipase genes
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US7241463B2 (en) 2000-09-25 2007-07-10 Novozymes A/S Methods for processing crustacean material
US6660491B2 (en) * 2000-11-24 2003-12-09 Ikeda Food Research Co., Ltd. Process for producing dietary sterol fatty acid esters
MXPA03007393A (es) 2001-02-21 2004-03-16 Novozymes As Produccion de productos alimenticios almidonosos.
US6645749B2 (en) * 2001-05-25 2003-11-11 Novozymes A/S Lipolytic enzyme
US7630836B2 (en) 2001-05-30 2009-12-08 The Kitasato Institute Polynucleotides
DE50115482D1 (de) 2001-07-11 2010-06-24 Cognis Ip Man Gmbh Lipase/Acyltransferase aus Candida parapsilosis
ATE342374T1 (de) 2001-08-22 2006-11-15 Haerting Thomas Francis Verfahren zur herstellung von sterol- und stanolestern mittels enzymatischer transesterifikation in lösungsmittel- und wasserfreien medien
AU2003215527A1 (en) * 2002-04-10 2003-10-27 Novozymes A/S Bacillus licheniformis mutant host cell
US7226771B2 (en) * 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
SE0201581D0 (sv) * 2002-05-29 2002-05-29 Scandinavian Biotechnology Res New improved acyltransferase
WO2003102118A2 (en) * 2002-05-30 2003-12-11 Council Of Scientific And Industrial Research Process for the pre-treatment of vegetable oils for physical refining
CA2490944C (en) 2002-07-03 2012-05-15 Novozymes A/S Treatment of dough with a lipoxygenase and a lipolytic enzyme
DK2338986T3 (en) 2002-08-19 2015-01-19 Dsm Ip Assets Bv Novel lipases and uses thereof
GB2379165A (en) 2002-10-22 2003-03-05 Dsm Nv Animal feed
JP4300839B2 (ja) 2002-11-14 2009-07-22 セイコーエプソン株式会社 画像処理装置、画像処理方法および画像処理プログラム
US20060035800A1 (en) 2002-12-11 2006-02-16 Novozymes A/S Detergent composition
WO2004053152A1 (en) 2002-12-12 2004-06-24 Novozymes A/S Method of selecting a lipolytic enzyme
JP2006511725A (ja) 2002-12-23 2006-04-06 ノボザイムス ノース アメリカ,インコーポレイティド ポリエステル布の処理方法
MXPA05007654A (es) * 2003-01-17 2005-09-30 Danisco Metodo.
US7955814B2 (en) * 2003-01-17 2011-06-07 Danisco A/S Method
BRPI0409599B1 (pt) 2003-04-28 2014-09-16 Novozymes As Sequência de ácido nucléico, construto de ácido nucléico, vetor de expressão recombinante, micro-organismo recombinante, composição de massa, métodos de produzir uma fosfolipase, de preparar uma massa ou um produto assado feito da massa, processos para reduzir o conteúdo de fósforo em um óleo vegetal e para produzir queijo
US20060251763A1 (en) 2003-06-19 2006-11-09 Patkar Shamkant A Phospholipase variants
WO2005005977A2 (en) 2003-06-30 2005-01-20 Applera Corporation Fluorescent phospholipase assays and compositions
BRPI0412401A (pt) 2003-07-07 2006-09-05 Genencor Int polipeptìdeos da amilase exoespecìficos, ácidos nucléicos que codificam tais polipeptìdeos e usos dos mesmos
JP4327536B2 (ja) 2003-09-01 2009-09-09 レシップ株式会社 券硬貨投入口及びそれを備えた券硬貨分離装置
WO2005056782A2 (en) 2003-12-03 2005-06-23 Genencor International, Inc. Perhydrolase
KR101169265B1 (ko) 2003-12-24 2012-08-02 대니스코 에이/에스 단백질
US7718408B2 (en) * 2003-12-24 2010-05-18 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
EP2284184A3 (en) 2004-01-09 2011-05-18 Novozymes, Inc. Bacillus YvmA inactivation
JP2007524744A (ja) 2004-02-24 2007-08-30 ノボザイムス アクティーゼルスカブ 液状洗剤中の酵素の安定化
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
AU2005223969B2 (en) 2004-03-24 2010-08-05 Novozymes A/S Process for producing cheese
CA2562171A1 (en) 2004-04-08 2005-11-24 Genencor International, Inc. Mutant .alpha.-amylases
WO2006008508A1 (en) * 2004-07-16 2006-01-26 Danisco A/S Enzymatic oil-degumming method
DE102004040134A1 (de) 2004-08-19 2006-02-23 Henkel Kgaa Neue essentielle Gene von Bacillus licheniformis und darauf aufbauende verbesserte biotechnologische Produktionsverfahren
US7811979B2 (en) 2004-09-21 2010-10-12 Novozymes A/S Subtilases
CN101084307B (zh) * 2004-09-30 2011-06-01 诺维信股份有限公司 具有脂肪酶活性的多肽和编码其的多核苷酸
DE102005039836A1 (de) 2005-08-23 2007-03-01 Cognis Ip Management Gmbh Sterolesterpulver
EP1788080A1 (en) 2005-11-22 2007-05-23 Süd-Chemie Ag Use of a thermostable phospholipase in the degumming of an oil or fat, and a method for obtaining a thermostable phopholipase
CN101200754B (zh) 2007-12-07 2010-06-02 中国农业科学院油料作物研究所 无溶剂系统中固定化全细胞酶催化生产植物甾醇酯的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2403025A1 (en) * 2002-10-08 2004-04-08 Cognis Deutschland Gmbh & Co. Kg Enzymes with lipase/acyltransferase activity
WO2004064987A2 (en) * 2003-01-17 2004-08-05 Danisco A/S Method
CN1898386A (zh) * 2003-12-24 2007-01-17 丹尼斯科公司 蛋白质

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