ATE494295T1 - Verfahren zur nukleinsäureamplifikation - Google Patents

Verfahren zur nukleinsäureamplifikation

Info

Publication number
ATE494295T1
ATE494295T1 AT05810422T AT05810422T ATE494295T1 AT E494295 T1 ATE494295 T1 AT E494295T1 AT 05810422 T AT05810422 T AT 05810422T AT 05810422 T AT05810422 T AT 05810422T AT E494295 T1 ATE494295 T1 AT E494295T1
Authority
AT
Austria
Prior art keywords
pcr
late
following
detection during
fluorescent dna
Prior art date
Application number
AT05810422T
Other languages
German (de)
English (en)
Inventor
Lawrence Wangh
John Rice
Aquiles Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Cristina Hartshorn
Original Assignee
Univ Brandeis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36203686&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE494295(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Brandeis filed Critical Univ Brandeis
Application granted granted Critical
Publication of ATE494295T1 publication Critical patent/ATE494295T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT05810422T 2004-10-18 2005-10-17 Verfahren zur nukleinsäureamplifikation ATE494295T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

Publications (1)

Publication Number Publication Date
ATE494295T1 true ATE494295T1 (de) 2011-01-15

Family

ID=36203686

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05810422T ATE494295T1 (de) 2004-10-18 2005-10-17 Verfahren zur nukleinsäureamplifikation

Country Status (17)

Country Link
US (3) US7632642B2 (cg-RX-API-DMAC7.html)
EP (3) EP2351766B1 (cg-RX-API-DMAC7.html)
JP (2) JP5543065B2 (cg-RX-API-DMAC7.html)
KR (3) KR101447405B1 (cg-RX-API-DMAC7.html)
CN (1) CN101076607B (cg-RX-API-DMAC7.html)
AT (1) ATE494295T1 (cg-RX-API-DMAC7.html)
AU (1) AU2005295298B2 (cg-RX-API-DMAC7.html)
BR (1) BRPI0517105B1 (cg-RX-API-DMAC7.html)
CA (3) CA2996947C (cg-RX-API-DMAC7.html)
DE (1) DE602005025791D1 (cg-RX-API-DMAC7.html)
DK (1) DK1805199T3 (cg-RX-API-DMAC7.html)
ES (3) ES2359322T3 (cg-RX-API-DMAC7.html)
NO (1) NO20072506L (cg-RX-API-DMAC7.html)
PL (1) PL1805199T3 (cg-RX-API-DMAC7.html)
PT (1) PT1805199E (cg-RX-API-DMAC7.html)
RU (1) RU2460804C2 (cg-RX-API-DMAC7.html)
WO (1) WO2006044994A2 (cg-RX-API-DMAC7.html)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0517105B1 (pt) * 2004-10-18 2015-06-23 Univ Brandeis Método para análise de pelo menos um produto de amplificação fita simples de um processo de amplificação de ácido nucléico não-simétrico
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
CN101802224A (zh) 2007-07-12 2010-08-11 史密斯探测公司 光纤检测系统
US8592182B2 (en) 2007-10-23 2013-11-26 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
US9856522B2 (en) 2008-12-30 2018-01-02 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムｐｃｒ装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
EP2406394B1 (en) 2009-03-12 2014-01-08 Brandeis University Reagents and methods for pcr
US8674080B2 (en) 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077840A1 (es) 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
US8853373B2 (en) * 2009-08-14 2014-10-07 Hitachi, Ltd. Method and reagent for gene sequence analysis
EP2491144B1 (en) 2009-10-21 2015-09-30 Brandeis University Methods for analyzing single-stranded nucleic acid sequences
CN102712955A (zh) * 2009-11-03 2012-10-03 Htg分子诊断有限公司 定量核酸酶保护测序(qNPS)
SG185543A1 (en) * 2010-05-14 2012-12-28 Fluidigm Corp Assays for the detection of genotype, mutations, and/or aneuploidy
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
US20120058481A1 (en) * 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
WO2012033875A2 (en) 2010-09-08 2012-03-15 Brandeis University Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
IN2013MN00522A (cg-RX-API-DMAC7.html) 2010-09-24 2015-05-29 Univ Leland Stanford Junior
WO2012048207A2 (en) * 2010-10-07 2012-04-12 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
ES2689257T3 (es) 2010-11-10 2018-11-12 Brandeis University Kit para la detección e identificación de micobacterias
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
US20140004504A1 (en) * 2010-12-10 2014-01-02 Brandeis University Compositions and methods for the detection and analysis of african swine fever virus
EP3181704B1 (en) 2010-12-27 2021-01-27 Abbott Molecular Inc. Quantitating high titer samples by digital pcr
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
WO2012151111A1 (en) 2011-05-04 2012-11-08 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
US20150031024A1 (en) * 2011-07-30 2015-01-29 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
CA2849023C (en) 2011-09-15 2022-07-19 David A. Shafer Probe:antiprobe compositions for high specificity dna or rna detection
CN104024433B (zh) * 2011-10-31 2016-11-16 爱科来株式会社 基因丰度的测定方法
CN107326085B (zh) 2012-02-03 2022-05-03 加州理工学院 多路生化测定中信号的编码和解码
EP2820157B1 (en) 2012-03-02 2019-05-01 Winthrop-University Hospital Method for using probe based pcr detection to measure the levels of circulating demethylated beta cell derived dna as a measure of beta cell loss in diabetes
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
WO2014022827A1 (en) * 2012-08-03 2014-02-06 California Institute Of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
EP2895627A4 (en) * 2012-09-17 2016-06-29 Univ Brandeis COMBINATION OF DSDNA BINDING DYES AND PROBES FOR CHARACTERIZING SSDNA SEQUENCES
US20160186267A1 (en) * 2013-02-21 2016-06-30 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
EP3666903B1 (en) * 2013-08-12 2025-12-31 Bio-Rad Laboratories, Inc. AMPLIFICATION REPORTER GENE CONTAINING OLIGOMERS WITH BASIC PAIRINGS
WO2015047186A1 (en) * 2013-09-30 2015-04-02 Di Wu Methods to profile molecular complexes by using proximity bar-coding
WO2015086604A1 (en) * 2013-12-11 2015-06-18 Qiagen Gmbh Nucleic acid detection and quantification
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170247745A1 (en) * 2014-09-12 2017-08-31 Click Diagnostics, Inc. Multiplex optical detection
ES2994737T3 (en) 2014-10-10 2025-01-30 Univ Rutgers Polymerase chain reaction primers and probes for mycobacterium tuberculosis
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
CA2971200A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
ES2898103T3 (es) 2014-12-31 2022-03-03 Visby Medical Inc Dispositivos para pruebas de diagnóstico molecular
US10913944B2 (en) * 2015-01-12 2021-02-09 Life Technologies Corporation Methods, systems and compositions thereof for nucleic acid library quality control and quantification
EP3353697B1 (en) * 2015-09-24 2025-11-05 Seegene, Inc. Multiple dataset analysis for determining the presence or absence of target analyte
WO2017087942A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting rna and dna sequences comprising high levels of intramolecular hybridization
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
WO2017209563A1 (en) * 2016-06-02 2017-12-07 Seegene, Inc. . Method for detecting a target analyte in a sample using a signal change-amount data set
EP3478857A1 (en) 2016-06-29 2019-05-08 Click Diagnostics, Inc. Devices and methods for the detection of molecules using a flow cell
WO2018190814A1 (en) * 2017-04-11 2018-10-18 Nugen Technologies, Inc. Library quantitation and qualification
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
KR102380264B1 (ko) * 2017-08-31 2022-03-29 주식회사 씨젠 다이머-형성 프라이머 쌍을 이용한 구성요소의 성능 평가
MX2020004783A (es) 2017-11-09 2020-08-13 Visby Medical Inc Dispositivo portatil para el diagnostico molecular y metodos para la deteccion de virus objetivo.
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅（武汉）生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
CA3088250A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
WO2019203623A1 (en) * 2018-04-20 2019-10-24 Seegene, Inc. . Method and apparatus for detecting a plurality of target nucleic acid sequences in sample
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
KR20210081335A (ko) 2018-09-03 2021-07-01 비스비 메디컬, 인코포레이티드 항생제 감수성 시험을 위한 장치 및 방법
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
US12263478B2 (en) 2019-04-28 2025-04-01 Visby Medical, Inc. Molecular diagnostic devices with digital detection capability and wireless connectivity
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
PL4034674T3 (pl) 2019-09-23 2025-08-04 Universiteit Gent Sonda i sposób genotypowania str
EP4085149A4 (en) 2020-01-03 2024-03-06 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术（深圳）有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
US20230068047A1 (en) * 2021-08-19 2023-03-02 Luminex Corporation Digital amplification assay analysis method
CN119173642A (zh) * 2022-06-20 2024-12-20 株式会社日立高新技术 引物、dna检测方法和dna检测试剂盒

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
CA2591550C (en) * 1996-06-04 2009-04-28 University Of Utah Research Foundation Monitoring hybridization during pcr
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
WO2001013086A2 (en) 1999-08-13 2001-02-22 Brandeis University Detection of nucleic acids
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US7662550B1 (en) 1999-10-22 2010-02-16 Phri Properties, Inc. Assays for short sequence variants
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
JP2005514003A (ja) * 2001-06-08 2005-05-19 シャンハイ メンデル ディーエヌエー センター シーオー．， エルティーディー 高いプライミング特異性を有するｄｎａの低温サイクル伸長
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
WO2003040397A2 (en) * 2001-10-25 2003-05-15 Gorilla Genomics, Inc. Asymmetric pcr with nuclease-free polymerase or nuclease-resistant molecular beacons
WO2003045233A1 (en) 2001-11-21 2003-06-05 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
ATE378425T1 (de) * 2002-09-02 2007-11-15 Toyo Boseki Verfahren zu identifizierung von nukleotid- polymorphismen unter verwendung von resonanzenergietransfer
WO2004048516A2 (en) * 2002-11-22 2004-06-10 North Shore-Long Island Jewish Research Institute Elf3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
CA2548205C (en) * 2003-12-03 2017-06-27 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
ES2741546T3 (es) * 2004-03-31 2020-02-11 Massachusetts Gen Hospital Método para determinar la respuesta del cáncer a tratamientos dirigidos al receptor del factor de crecimiento epidérmico
BRPI0517105B1 (pt) * 2004-10-18 2015-06-23 Univ Brandeis Método para análise de pelo menos um produto de amplificação fita simples de um processo de amplificação de ácido nucléico não-simétrico

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