DE602005025791D1 - Verfahren zur nukleinsäureamplifikation - Google Patents

Verfahren zur nukleinsäureamplifikation

Info

Publication number
DE602005025791D1
DE602005025791D1 DE602005025791T DE602005025791T DE602005025791D1 DE 602005025791 D1 DE602005025791 D1 DE 602005025791D1 DE 602005025791 T DE602005025791 T DE 602005025791T DE 602005025791 T DE602005025791 T DE 602005025791T DE 602005025791 D1 DE602005025791 D1 DE 602005025791D1
Authority
DE
Germany
Prior art keywords
pcr
late
following
detection during
fluorescent dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005025791T
Other languages
English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Cristina Hartshorn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36203686&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602005025791(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Brandeis University filed Critical Brandeis University
Publication of DE602005025791D1 publication Critical patent/DE602005025791D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
DE602005025791T 2004-10-18 2005-10-17 Verfahren zur nukleinsäureamplifikation Active DE602005025791D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

Publications (1)

Publication Number Publication Date
DE602005025791D1 true DE602005025791D1 (de) 2011-02-17

Family

ID=36203686

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005025791T Active DE602005025791D1 (de) 2004-10-18 2005-10-17 Verfahren zur nukleinsäureamplifikation

Country Status (18)

Country Link
US (3) US7632642B2 (de)
EP (3) EP1805199B1 (de)
JP (2) JP5543065B2 (de)
KR (3) KR101447405B1 (de)
CN (1) CN101076607B (de)
AT (1) ATE494295T1 (de)
AU (1) AU2005295298B2 (de)
BR (1) BRPI0517105B1 (de)
CA (3) CA2996947C (de)
DE (1) DE602005025791D1 (de)
DK (1) DK1805199T3 (de)
ES (3) ES2668467T3 (de)
HK (1) HK1106251A1 (de)
NO (1) NO20072506L (de)
PL (1) PL1805199T3 (de)
PT (1) PT1805199E (de)
RU (1) RU2460804C2 (de)
WO (1) WO2006044994A2 (de)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL1805199T3 (pl) * 2004-10-18 2011-06-30 Univ Brandeis Sposoby amplifikacji kwasu nukleinowego
CN100487432C (zh) * 2006-06-22 2009-05-13 上海交通大学 采用分子信标对核酸信号进行恒温放大与检测的方法
JP2010533297A (ja) 2007-07-12 2010-10-21 スミスズ ディテクション インコーポレイティド 光ファイバ検出システム
EP2215259A1 (de) * 2007-10-23 2010-08-11 Stratos Genomics Inc. Nukleinsäuresequenzierung mit hohem durchsatz mittels spacing
US8538733B2 (en) * 2008-01-25 2013-09-17 Life Technologies Corporation Methods for the analysis of dissociation melt curve data
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
WO2010076189A1 (en) 2008-12-30 2010-07-08 Stmicroelectronics S.R.L. Method, microreactor and apparatus for carrying out real-time nucleic acid amplification
FR2940805B1 (fr) * 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
JP5423006B2 (ja) * 2009-01-20 2014-02-19 ソニー株式会社 プライマー評価方法、プライマー評価プログラム及びリアルタイムpcr装置
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CA2755207A1 (en) 2009-03-12 2010-09-16 Brandeis University Reagents and methods for pcr
US8674080B2 (en) * 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
DE102009026651A1 (de) * 2009-06-02 2011-02-17 Großmann, Kay, Dr. rer. nat. (Chemie) Verfahren und Messvorrichtung zur qualitativen und quantitativen Analyse von Körperflüssigkeiten
AR077840A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Ensayos de deteccion e identificacion de especies y tipos de staphylococcus
AR077841A1 (es) * 2009-08-11 2011-09-28 Univ Brandeis Metodos kits y composiciones de deteccion de acido nucleico a multiples temperaturas, con sonda unica
CN102471364B (zh) * 2009-08-14 2015-01-14 株式会社日立制作所 基因序列分析的方法和试剂
US9353407B2 (en) 2009-10-21 2016-05-31 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
CN102712955A (zh) * 2009-11-03 2012-10-03 Htg分子诊断有限公司 定量核酸酶保护测序(qNPS)
WO2011142836A2 (en) * 2010-05-14 2011-11-17 Fluidigm Corporation Assays for the detection of genotype, mutations, and/or aneuploidy
IT1405994B1 (it) * 2010-06-22 2014-02-06 Euroclone Spa Metodo ad elevata sensibilita' per rilevare un acido nucleico target in un campione
US20120058481A1 (en) * 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
US9850529B2 (en) 2010-09-08 2017-12-26 Brandeis University Compositions and methods for nucleic acid based diagnostic assays for variable sequence targets
EP3572528A1 (de) 2010-09-24 2019-11-27 The Board of Trustees of the Leland Stanford Junior University Direkte erfassung, amplifikation und sequenzierung einer ziel-dna unter verwendung immobilisierter primer
US20130295570A1 (en) * 2010-10-07 2013-11-07 Brandeis University Compositions and methods for nucleic acid based diagnostic assays
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
ES2689257T3 (es) 2010-11-10 2018-11-12 Brandeis University Kit para la detección e identificación de micobacterias
US9637790B2 (en) 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA
WO2012079016A1 (en) * 2010-12-10 2012-06-14 Brandeis University Compositions and methods for the detection and analysis of african swine fever virus
EP3839064A1 (de) 2010-12-27 2021-06-23 Abbott Molecular Inc. Systeme zur quantifizierung von proben mit hohem titer mittels digitaler pcr
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
CN103649335B (zh) 2011-05-04 2015-11-25 Htg分子诊断有限公司 定量核酸酶保护测定(qnpa)和测序(qnps)的改进
US20150031024A1 (en) * 2011-07-30 2015-01-29 The Regents Of The University Of California Enzymatic preparation of 10 base to 50 kb double-strand dna reagent for sequencing with a nanopore-polymerase sequencing device
US20130052650A1 (en) 2011-08-29 2013-02-28 Thermo Fisher Scientific Inc. Dye blends
CN102321765B (zh) * 2011-09-08 2014-04-02 厦门基科生物科技有限公司 一种实时荧光pcr方法及用途
ES2684525T3 (es) 2011-09-15 2018-10-03 David A. Shafer Composiciones de sonda:antisonda para detección de ADN o ARN de alta especificidad
CN114717296A (zh) 2012-02-03 2022-07-08 加州理工学院 多路生化测定中信号的编码和解码
EP2820157B1 (de) 2012-03-02 2019-05-01 Winthrop-University Hospital Verfahren zur verwendung von sondenbasierter pcr-detektion zum messen der konzentration von dna aus zirkulierenden entmethylierten beta-zellen als massstab für beta-zell-verlust bei diabetes
CN102643910B (zh) * 2012-04-10 2014-10-15 中国人民解放军第三军医大学第一附属医院 非对称多色荧光发夹探针链反应在病原菌检测中的应用
EP2880184B1 (de) 2012-08-03 2021-03-31 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
EP2895627A4 (de) * 2012-09-17 2016-06-29 Univ Brandeis Kombination aus dsdna-bindendem farbstoff und sonden zur charakterisierung von ssdna-sequenzen
SG11201506660RA (en) * 2013-02-21 2015-09-29 Toma Biosciences Inc Methods, compositions, and kits for nucleic acid analysis
CN105339505B (zh) * 2013-03-12 2021-08-10 生命技术公司 基于通用报告体的基因分型方法和材料
WO2015023677A1 (en) * 2013-08-12 2015-02-19 Bio-Rad Laboratories, Inc. Amplification reporter with base-pairing oligomers
EP3052658B1 (de) * 2013-09-30 2021-03-31 Vesicode AB Verfahren zur profilierung von molekularen komplexen mittels näherungsabhängiger barcodierung
US10501783B2 (en) 2013-12-11 2019-12-10 Qiagen Gmbh Nucleic acid detection and quantification
US10195610B2 (en) 2014-03-10 2019-02-05 Click Diagnostics, Inc. Cartridge-based thermocycler
GB201411567D0 (en) 2014-06-30 2014-08-13 Epistem Ltd Quantification methods
US20170247745A1 (en) * 2014-09-12 2017-08-31 Click Diagnostics, Inc. Multiplex optical detection
CA2964265A1 (en) 2014-10-10 2016-04-14 Rutgers, The State University Of New Jersey Polymerase chain reaction primers and probes for mycobacterium tuberculosis
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
JP2017537648A (ja) * 2014-12-19 2017-12-21 ブランデイズ ユニバーシティー ミスプライミング防止試薬
CN114958990A (zh) 2014-12-31 2022-08-30 维斯比医学公司 使用分子诊断测试装置检测靶核酸的方法
EP3748002A1 (de) * 2015-01-12 2020-12-09 Life Technologies Corporation Verfahren, systeme und zusammensetzungen daraus zur kontrolle und quantifizierung der nukleinsäurebibliotheksqualität
WO2017052300A1 (en) * 2015-09-24 2017-03-30 Seegene, Inc. . Multiple dataset analysis for determining the presence or absence of target analyte
WO2017087943A1 (en) 2015-11-20 2017-05-26 Brandeis University Amplifying and detecting coconut cadang-cadang viroid rna
WO2017197040A1 (en) 2016-05-11 2017-11-16 Click Diagnostics, Inc. Devices and methods for nucleic acid extraction
CN109923613B (zh) * 2016-06-02 2023-07-04 Seegene株式会社 利用信号变化量数据集的样品内的目标分析物质检测方法
MX2018015889A (es) 2016-06-29 2019-05-27 Click Diagnostics Inc Dispositivos y metodos para la deteccion de moleculas usando una celda de flujo.
JP6983906B2 (ja) * 2017-04-11 2021-12-17 ニューゲン テクノロジーズ, インコーポレイテッド ライブラリーの定量および定性
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
EP3676400A4 (de) * 2017-08-31 2021-06-02 Seegene, Inc. Bewertung der leistung von komponenten unter verwendung eines paares von dimerbildenden primern
JP7239568B2 (ja) 2017-11-09 2023-03-14 ビスビュー メディカル,インコーポレイテッド 携帯型分子診断デバイスおよび標的ウイルスの検出方法
CN108570495A (zh) * 2018-01-15 2018-09-25 新开源博畅(武汉)生物科技有限公司 一种hla高分辨基因位点的快速扩增诊断试剂盒及扩增方法
CN108624664B (zh) * 2018-01-16 2020-08-28 武汉菲思特生物科技有限公司 一种急性肝损伤的早期诊断标志物的快速扩增方法
CA3088250A1 (en) * 2018-01-22 2019-07-25 Luminex Corporation Methods and compositions for discrete melt analysis
BR112020019755A2 (pt) * 2018-04-20 2021-01-26 Seegene, Inc. método e aparelho para detectar uma pluralidade de sequências de ácidos nucleicos alvo na amostra
CN109321672B (zh) * 2018-10-30 2021-07-06 中国农业大学 核苷酸组合物、试剂盒及检测方法
WO2021011850A2 (en) * 2019-07-17 2021-01-21 Chao Chien Chung Amplification assay for the detection of anaplasma phagocytophilum
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
CA3155451A1 (en) 2019-09-23 2021-04-01 Universiteit Gent Probe and method for str-genotyping
WO2021138544A1 (en) 2020-01-03 2021-07-08 Visby Medical, Inc. Devices and methods for antibiotic susceptibility testing
US20230242971A1 (en) * 2020-05-08 2023-08-03 Roche Sequencing Solutions, Inc. Removal of excess oligonucleotides from a reation mixture
CN111961717B (zh) * 2020-08-28 2023-08-01 南方医科大学 单管同时检测缺失型和非缺失型α-地中海贫血基因的荧光PCR试剂盒
CN111909990B (zh) * 2020-08-28 2023-11-28 亚能生物技术(深圳)有限公司 单管同时检测基因的缺失突变和点突变的荧光pcr检测方法
US20230068047A1 (en) * 2021-08-19 2023-03-02 Luminex Corporation Digital amplification assay analysis method
WO2023248310A1 (ja) * 2022-06-20 2023-12-28 株式会社日立ハイテク プライマー、dna検出方法およびdna検出キット

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5756285A (en) * 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5716784A (en) * 1996-02-05 1998-02-10 The Perkin-Elmer Corporation Fluorescence detection assay for homogeneous PCR hybridization systems
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
EP1179600B1 (de) * 1996-06-04 2005-05-11 University Of Utah Research Foundation Überwachung der Hybridisierung während PCR
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
WO2001013086A2 (en) 1999-08-13 2001-02-22 Brandeis University Detection of nucleic acids
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
CA2387306C (en) * 1999-10-22 2010-04-27 The Public Health Research Institute Of The City Of New York, Inc. Assays for short sequence variants
US20030082550A1 (en) * 2000-09-08 2003-05-01 Hans-Ulrich Thomann Mutations of the cyclooxygenase-2 gene
US7267945B2 (en) * 2001-03-26 2007-09-11 Applera Corporation Methods of determining the presence of polynucleotides employing amplification
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
RU2004100107A (ru) * 2001-06-08 2005-06-10 Шанхай Мендель Дна Сентер Ко., Лтд (Cn) Низкотемпературное циклическое удлинение днк с высокой специфичностью праймирования
US20030108906A1 (en) * 2001-07-27 2003-06-12 Brooksbank Robert Alan Identification and use of molecules implicated in pain
CA2462505A1 (en) * 2001-10-25 2003-05-15 Gorilla Genomics, Inc. Asymmetric pcr with nuclease-free polymerase or nuclease-resistant molecular beacons
AU2002346484A1 (en) 2001-11-21 2003-06-10 Optiscan Biomedical Corporation Method and apparatus for improving the accuracy of alternative site analyte concentration measurements
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
JP2004024035A (ja) * 2002-06-21 2004-01-29 Tosoh Corp 核酸の検出方法
ATE378425T1 (de) * 2002-09-02 2007-11-15 Toyo Boseki Verfahren zu identifizierung von nukleotid- polymorphismen unter verwendung von resonanzenergietransfer
US7718783B2 (en) * 2002-11-22 2010-05-18 Kaplan Mark H ELF3 gene compositions and methods
US20040175704A1 (en) * 2003-03-06 2004-09-09 Stratagene Compositions and methods for polynucleotide sequence detection
JP5162133B2 (ja) * 2003-12-03 2013-03-13 アボット・ラボラトリーズ 二本鎖直鎖核酸プローブ及びその使用
WO2005094357A2 (en) * 2004-03-31 2005-10-13 The General Hospital Corporation Method to determine responsiveness of cancer to epidermal growth factor receptor targeting treatments
PL1805199T3 (pl) * 2004-10-18 2011-06-30 Univ Brandeis Sposoby amplifikacji kwasu nukleinowego

Also Published As

Publication number Publication date
CA2584569A1 (en) 2006-04-27
PT1805199E (pt) 2011-04-11
DK1805199T3 (da) 2011-04-26
KR101347951B1 (ko) 2014-01-07
JP5543065B2 (ja) 2014-07-09
EP1805199A2 (de) 2007-07-11
ATE494295T1 (de) 2011-01-15
KR101447405B1 (ko) 2014-10-06
KR20130021465A (ko) 2013-03-05
KR101424812B1 (ko) 2014-08-07
WO2006044994A2 (en) 2006-04-27
BRPI0517105B1 (pt) 2015-06-23
KR20130113541A (ko) 2013-10-15
AU2005295298A1 (en) 2006-04-27
WO2006044994A3 (en) 2007-03-08
US20060177841A1 (en) 2006-08-10
NO20072506L (no) 2007-07-11
EP2351766B1 (de) 2014-12-03
EP2927238B1 (de) 2018-01-17
KR20070090159A (ko) 2007-09-05
US9745624B2 (en) 2017-08-29
JP2014110793A (ja) 2014-06-19
EP1805199B1 (de) 2011-01-05
US20130095479A1 (en) 2013-04-18
US7632642B2 (en) 2009-12-15
EP2351766A3 (de) 2012-03-21
EP2927238A1 (de) 2015-10-07
CA2996947C (en) 2019-02-26
ES2529204T3 (es) 2015-02-17
JP5860484B2 (ja) 2016-02-16
CA2855748A1 (en) 2006-04-27
JP2008516612A (ja) 2008-05-22
HK1106251A1 (en) 2008-03-07
CA2996947A1 (en) 2006-04-27
CN101076607A (zh) 2007-11-21
RU2007118545A (ru) 2008-11-27
CA2584569C (en) 2014-09-30
RU2460804C2 (ru) 2012-09-10
AU2005295298B2 (en) 2011-07-14
CN101076607B (zh) 2013-04-24
US20120040352A1 (en) 2012-02-16
ES2359322T3 (es) 2011-05-20
ES2668467T3 (es) 2018-05-18
CA2855748C (en) 2018-08-28
EP2351766A2 (de) 2011-08-03
EP1805199A4 (de) 2008-07-16
BRPI0517105A (pt) 2008-09-30
PL1805199T3 (pl) 2011-06-30

Similar Documents

Publication Publication Date Title
DE602005025791D1 (de) Verfahren zur nukleinsäureamplifikation
JP2985446B2 (ja) 核酸の検出及び測定方法
JP4982387B2 (ja) 微生物のゲノムdnaを同定するデバイスおよび方法
CN107385040B (zh) 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
JP3785517B2 (ja) 核酸融解温度測定法
ATE384803T1 (de) Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens
CN111270007A (zh) 猪瘟病毒检测用引物、微流控芯片、系统及其应用
DE60204874D1 (de) Verfahren zur nachweis von nukleinsäuren
TWI465572B (zh) Method, composition and system of amplification and detection of target microbial DNA
EP3036341B1 (de) Nachweis von nukleinsäureamplifikation in einem porösen substrat
JP2002027993A (ja) 特定の核酸を検出定量するための等長プライマー伸長法およびキット
CN112921120B (zh) 一种可重复利用的核酸荧光探针及相应的核酸检测方法
CN108642147A (zh) 一种快速扩增核酸的方法
KR101334072B1 (ko) 핵산 정량 방법 및 키트
EP0877094A2 (de) Nachweis und Quantifizierung von einen oder mehreren Nukleotidsequenzen durch Benutzung von räumlich lokalisierte Target Replikation
US20130115616A1 (en) Detection of nucleic acids by agglutination
Powell et al. Tissue source attribution using the PyroMark® Q48 Autoprep System: Sperm identification in forensic casework
KR101356396B1 (ko) Pcr 수행 없는 소위성 주형 반복 유전자 타이핑법
DE602005019205D1 (de) Analytische zusammensetzung und verfahren
RU2644262C1 (ru) Способ детекции множества последовательностей нуклеотидов в одном образце методом кодирования ампликонов при проведении полимеразной цепной реакции
Saito et al. Identification of animal species by nucleic acid chromatography of hair samples and investigation of its applicability to human biological samples
Wang et al. Establishment of an ultrasensitive and visual detection platform for Neospora caninum based-on the RPA-CRISPR/Cas12a system
CN114657237A (zh) 一种pcr终点可视化检测核酸的方法
CN117417986A (zh) 一种基于5-Br-PAPS快速检测靶标核酸序列的方法
WO2005001124A3 (en) Methods of nucleic acid analysis