BRPI0517105A - primers, sondas e métodos para amplificação de ácido nucléico - Google Patents

primers, sondas e métodos para amplificação de ácido nucléico

Info

Publication number
BRPI0517105A
BRPI0517105A BRPI0517105-9A BRPI0517105A BRPI0517105A BR PI0517105 A BRPI0517105 A BR PI0517105A BR PI0517105 A BRPI0517105 A BR PI0517105A BR PI0517105 A BRPI0517105 A BR PI0517105A
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BR
Brazil
Prior art keywords
pcr
probes
late
primers
following
Prior art date
Application number
BRPI0517105-9A
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English (en)
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
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Univ Brandeis
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=36203686&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0517105(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Brandeis filed Critical Univ Brandeis
Publication of BRPI0517105A publication Critical patent/BRPI0517105A/pt
Publication of BRPI0517105B1 publication Critical patent/BRPI0517105B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

PRIMERS, SONDAS E METODOS PARA .AMPLIFICAçAO DE áCIDO NUCLéICO. Detecção homogênea durante ou após amplificação por PCR, de preferência LATE-PCR, utilizando corante de DNA fluorescente e primers rotulados indiretamente excitáveis e sondas, melhora a reprodutibilidade e quantificação. Detecção homogênea em baixa temperatura durante ou após amplificação por PCR não-simétrica, de preferência LATE- PCR, utilizando corante de DNA fluorescente e sondas tolerantes à combinação errónea rotuladas indiretamente excitáveis permite análise de alvos complexos. Métodos de preparo de amostra de seqüenciamento após amplificações por LATE-PCR reduzem a complexidade e permitem processamento em "único tubo".
BRPI0517105-9A 2004-10-18 2005-10-17 Método para análise de pelo menos um produto de amplificação fita simples de um processo de amplificação de ácido nucléico não-simétrico BRPI0517105B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61965404P 2004-10-18 2004-10-18
US60/619,654 2004-10-18
PCT/US2005/037667 WO2006044994A2 (en) 2004-10-18 2005-10-17 Primers, probes and methods for nucleic acid amplification

Publications (2)

Publication Number Publication Date
BRPI0517105A true BRPI0517105A (pt) 2008-09-30
BRPI0517105B1 BRPI0517105B1 (pt) 2015-06-23

Family

ID=36203686

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0517105-9A BRPI0517105B1 (pt) 2004-10-18 2005-10-17 Método para análise de pelo menos um produto de amplificação fita simples de um processo de amplificação de ácido nucléico não-simétrico

Country Status (18)

Country Link
US (3) US7632642B2 (pt)
EP (3) EP2351766B1 (pt)
JP (2) JP5543065B2 (pt)
KR (3) KR101347951B1 (pt)
CN (1) CN101076607B (pt)
AT (1) ATE494295T1 (pt)
AU (1) AU2005295298B2 (pt)
BR (1) BRPI0517105B1 (pt)
CA (3) CA2584569C (pt)
DE (1) DE602005025791D1 (pt)
DK (1) DK1805199T3 (pt)
ES (3) ES2529204T3 (pt)
HK (1) HK1106251A1 (pt)
NO (1) NO20072506L (pt)
PL (1) PL1805199T3 (pt)
PT (1) PT1805199E (pt)
RU (1) RU2460804C2 (pt)
WO (1) WO2006044994A2 (pt)

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