Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
  • C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
  • C07D239/72—Quinazolines; Hydrogenated quinazolines
  • C07D239/86—Quinazolines; Hydrogenated quinazolines with hetero atoms directly attached in position 4
  • C07D239/94—Nitrogen atoms
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/06—Antipsoriatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P19/00—Drugs for skeletal disorders
  • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
  • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P27/00—Drugs for disorders of the senses
  • A61P27/02—Ophthalmic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Definitions

• PROPOXY)-QUINAZOLIN-6-YL]-ACRYLAMIDE AN IRREVERSIBLE
• This invention relates to the compound N-[4-(3-chloro-4-fluoro- phenylamino)-7-(3 -morpholin-4-yl-propoxy)-quinazolin-6-yl] -acrylamide that is an irreversible inhibitor of tyrosine kinases.
• This invention also relates to a method of treating cancer, atherosclerosis, restenosis, endometriosis, and psoriasis using the compound N-[4-(3-chloro-4-fluoro-phenylamino)-7-(3-morpholin-4-yl- propoxy)-quinazolin-6-yl] -acrylamide, and to a pharmaceutical composition that comprises the compound N-[4 ⁇ (3-chloro-4-fluoro-phenylamino)-7-(3-mo ⁇ pholin- 4-yl-propoxy)-quinazolin-6-yl]-acrylamide.
• Cancer has been viewed as a disease of the intracellular signaling system, or signal transduction mechanism.
• Cells receive instructions from many extracellular sources, instructing them to either proliferate or not to proliferate.
• the purpose of the signal transduction system is to receive these and other signals at the cell surface, get them into the cell, and then pass the signals on to the nucleus, the cytoskeleton, and transport and protein synthesis machinery.
• the most common cause of cancer is a series of defects, either in these proteins, when they are mutated, or in the regulation of the quantity of the protein in the cell such that it is over or under produced. Most often, there are key lesions in the cell which lead to a constitutive state whereby the cell nucleus receives a signal to proliferate, when this signal is not actually present.
• the cell may start to produce an authentic growth factor for its own receptors when it should not, the so-called autocrine loop mechanism. Mutations to the cell surface receptors, which usually signal into the cell by means of tyrosine kinases, can lead to activation of the kinase in the absence of ligand, and passing of a signal which is not really there. Alternatively, many surface kinases can be overexpressed on the cell surface leading to an inappropriately strong response to a weak signal. There are many levels inside the cell at which mutation or overexpression can lead to the same spurious signal arising in the cell, and there are many other kinds of signaling defects involved in cancer.
• This invention touches upon cancers which are driven by the three mechanisms just described, and which involve cell surface receptors of the epidermal growth factor receptor tyrosine kinase family (EGFR).
• EGFR epidermal growth factor receptor tyrosine kinase family
• This family consists of the EGF receptor (also known as erbBl), the erbB2 receptor, and its constitutively active oncoprotein mutant Neu, the erbB3 receptor and the erbB4 receptor.
• EGF receptor also known as erbBl
• erbB2 receptor the erbB2 receptor
• its constitutively active oncoprotein mutant Neu the erbB3 receptor
• erbB4 receptor the constitutively active oncoprotein mutant Neu
• other biological processes driven through members of the EGF family of receptors can also be treated by compounds of the invention described below.
• the EGFR has as its two most important ligands Epidermal Growth Factor (EGF) and Transforming Growth Factor alpha (TGF alpha).
• EGF Epidermal Growth Factor
• TGF alpha Transforming Growth Factor alpha
• the receptors appear to have only minor functions in adult humans, but are apparently implicated in the disease process of a large portion of all cancers, especially colon and breast cancer.
• the closely related erbB2 (HER2), erbB3 (HER3), and erbB4 (HER4) receptors have a family of Heregulins as their major ligands, and receptor overexpression and mutation have been unequivocally demonstrated as the major risk factor in poor prognosis breast cancer.
• restenosis is also a disease in which undesired cellular proliferation occurs. Restenosis involves the proliferation of vascular smooth muscle cells. Restenosis is a major clinical problem associated with coronary angioplasty and other medical procedures. Restenosis generally occurs within about 0 to 6 months in about 30% to 50% of patients who undergo balloon angioplasty to clear clogged coronary arteries in an effort to treat heart disease due to occluded arteries. The resulting restenosis causes substantial patient morbidity and health care expense.
• the process of restenosis is initiated by injury of the blood vessel, including arteries and veins, with the subsequent release of thrombogenic, vasoactive, and mitogenic factors.
• Endothelial and deep vessel injury leads to platelet aggregation, thrombus formation, inflammation, and activation of macrophages and smooth muscle cells.
• growth factors and cytokines which in turn may promote their own synthesis and release from target cells.
• a self-perpetuating process involving growth factors such as EGF, platelet derived growth factor (PDGF) or fibroblast growth factor (FGFs) is initiated.
• EGF EGF
• PDGF platelet derived growth factor
• FGFs fibroblast growth factor
• the proliferative skin disease psoriasis has no good cure at present. It is often treated by anticancer agents such as methotrexate, which have very serious side effects, and which are not very effective at the toxicity limited doses which have to be used. It is believed that TGF alpha is the major growth factor overproduced in psoriasis, since 50% of transgenic mice which over express TGF alpha develop psoriasis. This suggests that a good inhibitor of EGFR signalling could be used as antipsoriatic agent, preferably, but not necessarily, by topical dosing.
• irreversible tyrosine kinase inhibitors when compared to reversible inhibitors, because irreversible inhibitors can be used in prolonged suppression of the tyrosine kinase, limited only by the normal rate of receptor resynthesis, also called turnover.
• PCT Application Number PCT/US97/05778 (Publication Number WO 97/38983), which is hereby incorporated by reference, discloses compounds that are irreversible inhibitors of tyrosine kinases.
• Compound 1 has the following chemical structure:
• Example 21 the compound N-[4-[(3- bromophenyl)amino] -7- [3 -(4-morpholino)propoxy] quinazolin-6-yl] acrylamide, which has the following chemical structure:
• Compound 1 differs from Example 21 in the substitution of the phenyl ring that is attached to the quinoline group via a nitrogen atom.
• the phenyl ring of compound 1 is substituted at the 3 -position with chlorine and at the 4-position with fluorine.
• the phenyl ring of Example 21 of the PCT application (herein “Example 21") is substituted only at the 3-position with bromine. While these two compounds are similar in structure, compound 1 shows surprising and unexpected properties in vivo when compared to Example 21 of the PCT application.
• the present invention provides the compound N-[4-(3-chloro-4-fluoro- phenylamino)-7-(3-morpholin-4-yl-propoxy)-quinazolin-6-yl]-acrylamide, or the pharmaceutically acceptable salts thereof. Also provided is a pharmaceutically acceptable composition that comprises N- [4-(3 -chloro-4-fluoro-phenylamino)-7-(3 -morpholin-4-yl-propoxy)- quinazolin-6-yl] -acrylamide .
• the cancer is breast cancer.
• the cancer is colon cancer.
• Also provided is a method of irreversibly inhibiting tyrosine kinases comprising administering to a patient in need of tyrosine kinase inhibition a tyrosine kinase inhibiting amount of N-[4-(3-chloro-4-fluoro-phenylamino)-7- (3-morpholin-4-yl-propoxy)-quinazolin-6-yl]-acrylamide.
• the tyrosine kinase is EGFR.
• the tyrosine kinase is erbB2.
• the tyrosine kinase is erbB4. Also provided is a method of treating psoriasis, the method comprising administering to a patient having psoriasis a therapeutically effective amount of N-[4-(3-chloro-4-fluoro-phenylamino)-7-(3-mo ⁇ holin-4-yl-propoxy)-quinazolin- 6-yl] -acrylamide .
• the present invention provides the compound N-[4-(3-chloro-4-fluoro- phenylamino)-7-(3-mo ⁇ holin-4-yl-propoxy)-quinazolin-6-yl]-acrylamide (Compound 1), or the pharmaceutically acceptable salts thereof.
• Compound 1 is an irreversible inhibitor of tyrosine kinases, particularly
• EGFR tyrosine kinase Other tyrosine kinases that can be inhibited by compound 1 include FGFR, PDGFR, c-src, erbB2, and erbB4.
• a therapeutically effective amount of compound 1 can be administered to a patient having cancer, or a patient having restenosis or at risk of having restenosis, or a patient having psoriasis, atherosclerosis or at risk of having atherosclerosis, or endometriosis.
• patients having cancer restenosis, psoriasis, atherosclerosis, or endometriosis
• patients at risk of having restenosis are patients having undergone angioplasty, bypass, or graft procedures.
• patients at risk of developing atherosclerosis include patients who are obese, eat high fat diets, have high cholesterol levels, or have hypertension.
• patient means animals such as dogs, cats, cows, sheep, and also includes humans.
• cancer includes, but is not limited to, the following cancers: breast; ovary; cervix; prostate; testis; esophagus; glioblastoma; neuroblastoma; stomach; skin, keratoacanthoma; lung, epidermoid carcinoma, large cell carcinoma, adenocarcinoma; bone; colon, adenocarcinoma, adenoma; pancreas, adenocarcinoma; thyroid, follicular carcinoma, undifferentiated carcinoma, papillary carcinoma; seminoma; melanoma; sarcoma; bladder carcinoma; liver carcinoma and biliary passages; kidney carcinoma; myeloid disorders; lymphoid disorders, Hodgkins, hairy cells; buccal cavity and pharynx (oral), lip, tongue, mouth, pharynx; small intestine; colon-rectum, large intestine, rectum; brain and central nervous system; and leukemia.
• Preferred cancers that compound 1 can be used to treat include breast, colon, colon-rectum and ovarian cancers.
• compound 1 can be used to treat patients in need of inhibition vascular endothelial growth factor (VEGF) secretion.
• Patients in need of inhibition of VEGF secretion include those having cancer, diabetic retinopathy, rheumatoid arthritis, psoriasis, restenosis, atherosclerosis, osteoporosis, endometriosis, persons undergoing embryo implantation, or persons having other diseases in which angiogenesis or neovascularization plays a role.
• the compound of the present invention can be used to inhibit the tyrosine phosphorylation of erbB3.
• Compound 1 can be administered to humans and animals either orally, rectally, parenterally (intravenously, intramuscularly or subcutaneously), intracisternally, intravaginally, intraperitoneally, intravesically, locally (powders, ointments, or drops), or as a buccal or nasal spray.
• the compound can be administered alone or as part of a pharmaceutically acceptable composition that includes pharmaceutically acceptable excipients.
• compositions suitable for parenteral injection may comprise physiologically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile injectable solutions or dispersions.
• suitable aqueous and nonaqueous carriers, diluents, solvents, or vehicles include water, ethanol, polyols
• propyleneglycol, polyethyleneglycol, glycerol, and the like suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate.
• vegetable oils such as olive oil
• injectable organic esters such as ethyl oleate.
• Proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions and by the use of surfactants.
• compositions may also contain adjuvants such as preserving, wetting, emulsifying, and dispensing agents.
• adjuvants such as preserving, wetting, emulsifying, and dispensing agents.
• Prevention of the action of microorganisms can be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like.
• isotonic agents for example sugars, sodium chloride, and the like.
• Prolonged abso ⁇ tion of the injectable pharmaceutical form can be brought about by the use of agents delaying abso ⁇ tion, for example, aluminum monostearate and gelatin.
• Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules.
• the active compound is admixed with at least one inert customary excipient (or carrier) such as sodium citrate or dicalcium phosphate or
• fillers or extenders as for example, starches, lactose, sucrose, glucose, mannitol, and silicic acid;
• binders as for example, carboxymethylcellulose, alignates, gelatin, polyvinylpyrrolidone, sucrose, and acacia;
• humectants as for example, glycerol;
• disintegrating agents as for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain complex silicates, and sodium carbonate;
• solution retarders as for example paraffin;
• abso ⁇ tion accelerators as for example, quaternary ammonium compounds;
• compositions of a similar type may also be employed as fillers in soft- and hard-filled gelatin capsules using such excipients as lactose or milk sugar, as well as high molecular weight polyethylene-glycols, and the like.
• Solid dosage forms such as tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells, such as enteric coatings and others well- known in the art. They may contain opacifying agents, and can also be of such composition that they release the active compound in a certain part of the intestinal tract in a delayed manner. Examples of embedding compositions which can be used are polymeric substances and waxes. The active compound can also be in micro-encapsulated form, if appropriate, with one or more of the above-mentioned excipients.
• Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs.
• the liquid dosage forms may contain inert diluents commonly used in the art, such as water or other solvents, solubilizing agents and emulsifiers, as for example, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propyleneglycol, 1,3-butyleneglycol, dimethylformamide, oils, in particular, cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethyleneglycols and fatty acid esters of sorbitan or mixtures of these substances, and the like.
• inert diluents commonly used in the art, such as water or other solvents, solubilizing
• composition can also include adjuvants, such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
• adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.
• Suspensions in addition to the active compound, may contain suspending agents, as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar and tragacanth, or mixtures of these substances, and the like.
• suspending agents as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar and tragacanth, or mixtures of these substances, and the like.
• compositions for rectal administrations are preferably suppositories which can be prepared by mixing the compounds of the present invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethyleneglycol or a suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt in the rectum or vaginal cavity and release the active component.
• suitable non-irritating excipients or carriers such as cocoa butter, polyethyleneglycol or a suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt in the rectum or vaginal cavity and release the active component.
• Dosage forms for topical administration include ointments, powders, sprays, and inhalants.
• the active component is admixed under sterile conditions with a physiologically acceptable carrier and any preservatives, buffers, or propellants as may be required.
• Ophthalmic formulations, eye ointments, powders, and solutions are also contemplated as being within the scope of this invention.
• salts refers to those carboxylate salts, amino acid addition salts, esters, amides, and prodrugs of the compound of the present invention which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of patients without undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit/risk ratio, and effective for their intended use, as well as the zwitterionic forms, where possible, of the compounds of the invention.
• salts refers to the relatively non-toxic, inorganic and organic acid addition salts of compounds of the present invention.
• salts can be prepared in situ during the final isolation and purification of the compound or by separately reacting the purified compound in its free base form with a suitable organic or inorganic acid and isolating the salt thus formed.
• Representative salts include the hydrobromide, hydrochloride, sulfate, bisulfate, nitrate, acetate, oxalate, valerate, oleate, palmitate, stearate, laurate, borate, benzoate, lactate, phosphate, tosylate, citrate, maleate, fumarate, succinate, tartrate, naphthylate, mesylate, glucoheptonate, lactobionate and laurylsulphonate salts, and the like.
• alkali and alkaline earth metals such as sodium, lithium, potassium, calcium, magnesium, and the like
• non- toxic ammonium, quaternary ammonium, and amine cations including, but not limited to ammonium, tetramethylammomum, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, ethylamine and the like (see, for example, S.M. Berge, et al., "Pharmaceutical Salts," J. Pharm. Sci. , 1977;66: 1-19 which is inco ⁇ orated herein by reference).
• esters of the compounds of this invention examples include Cj-Cg alkyl esters wherein the alkyl group is a straight or branched chain. Acceptable esters also include C5-C7 cycloalkyl esters as well as arylalkyl esters such as, but not limited to benzyl. C1-C4 alkyl esters are preferred. Esters of the compound of the present invention may be prepared according to conventional methods.
• Examples of pharmaceutically acceptable, non-toxic amides of the compound of this invention include amides derived from ammonia, primary C1-C6 alkyl amines and secondary Cj-C ⁇ dialkyl amines wherein the alkyl groups are straight or branched chain. In the case of secondary amines, the amine may also be in the form of a 5- or 6-membered heterocycle containing one nitrogen atom. Amides derived from ammonia, C1-C3 alkyl primary amines and
• C1-C2 dialkyl secondary amines are preferred.
• Amides of the compound of the invention may be prepared according to conventional methods.
• prodrug refers to compounds that are rapidly transformed in vivo to yield the parent compound of the above formulas, for example, by hydrolysis in blood.
• a thorough discussion is provided in T. Higuchi and V. Stella, "Pro-drugs as Novel Delivery Systems,” Vol. 14 of the A.C.S. Symposium Series, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987, both of which are inco ⁇ orated herein by reference.
• the compound of the present invention can be administered to a patient at dosage levels in the range of about 0.1 to about 1,000 mg per day. For a normal human adult having a body weight of about 70 kg, a dosage in the range of about 0.01 to about 100 mg per kilogram of body weight per day is sufficient.
• the specific dosage used can vary. For example, the dosage can depend on a number of factors including the requirements of the patient, the severity of the condition being treated, and the pharmacological activity of the compound being used. The determination of optimum dosages for a particular patient is well- known to those skilled in the art.
• the compound of the present invention can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like. In general, the solvated forms are considered equivalent to the unsolvated forms for the pu ⁇ oses of the present invention.
• the compound 1 be either synthetically produced or biologically produced, such as through metabolism.
• Compound 1 can be synthesized as follows:
• Step A 4-[(3-Chloro-4-fluorophenyl amino1-7-fluoro-6-nitroquinazoline Powdered 4-chloro-7-fluoro-6-nitroquinazoline (J. Med. Chem., 1996;39:918) (82.77 g, 363.7 mmol) was added in batches over 10 minutes to a mechanically stirred solution of 3-chloro-4-fluoroaniline (53.183 g, 365.3 mmol) and N,N-dimethylaniline (88.5 g, 730 mmol) in isopropanol (1.09 L) kept under nitrogen on an ice-bath.
• the ice-bath was removed at the end of the addition, and the mixture was stirred at 25 °C for 6 hours.
• the mixture was then cooled again on an ice-bath, and water (200 mL), followed by aqueous Na2CO3 solution (10% w/v, 200 mL) were added dropwise with stirring. After a further 10 minutes, the mixture was Buchner filtered, and the residual solid was rinsed with dilute NaHCO3 solution (saturated/5, 2 x 100 mL), water (2 x 100 mL), and isopropanol
• Step B 4- [(3 -Chloro-4-fluorophenyl)amino] -7- [3 -(4-mo ⁇ holino propoxy] - 6-nitroquinazoline
• Step C 6-Amino-4-r(3-chloro-4-fluorophenyl)amino1-7-r3-(4- mo ⁇ holino)propoxy] quinazoline
• reaction mixture was celite filtered, and the solvent was removed under reduced pressure to give 6-amino-4-[(3-chloro-4-fluorophenyl)amino]-7-[3-(4- mo ⁇ holino)propoxy] quinazoline (57.81 g, 97.6% corrected) as a pale greenish solid.
• Step D N-[4-(3-chloro-4-fluoro-phenylamino)-7-(3-mo ⁇ holin-4-yl-propoxy)- quinazolin-6-yll-acrylamide
• reaction mixture was poured onto cracked ice (2 Kg), swirled, and gradually diluted with water (5 L). The mixture stood for 16 hours and was then Buchner filtered. The precipitate was rinsed with water (2 x 1 L), air dried for 18 hours, and then dried in a vacuum oven over
• Tissue Culture A431 human epidermoid carcinoma cells and MDA-MB-453 cells were obtained from the American Type Culture Collection, Rockville, MD and maintained as monolayers in dMEM (Dulbecco's modified eagle medium)/F12, 50:50 (Gibco/BRL, Bethesda, MD) containing 10% fetal bovine serum. The cells were grown at 37°C in a humidified atmosphere containing 5% CO2 in air.
• EGF receptor tyrosine kinase was isolated from A431 human epidermoid carcinoma cells by the following method. Cells were grown in roller bottles in dMEM/F12 media (Gibco/BRL, Bethesda, MD) containing 10% fetal calf serum.
• PMSF phenylmethylsulfonyl fluoride
• FGFR Fibroblast growth factor receptor
• PDGF platelet-derived growth factor
• insulin insulin and c-src tyrosine kinases were obtained by methods well-known to those skilled in the art.
• Enzyme assays for IC50 determinations were performed in 96-well filter plates (Millipore MADVN6550, Millipore, Bedford, MA). The total volume was 0.1 mL containing 20 mM Hepes, pH 7.4, 50 ⁇ M sodium vanadate, 40 mM magnesium chloride, 10 ⁇ M ATP containing 0.5 ⁇ Ci of [ 32 P]ATP, 20 ⁇ g of poly Glutamic acid/tyrosine (Sigma Chemical Co., St. Louis, MO), 10 ng of EGF receptor tyrosine kinase, and appropriate dilutions of inhibitor. All components except the ATP are added to the well and the plate incubated with shaking for
• the reaction is started by adding [ 32 P]ATP, and the plate is incubated at 25°C for 10 minutes.
• the reaction is terminated by addition of 0.1 mL of 20% trichloroacetic acid (TCA).
• TCA trichloroacetic acid
• A431 human epidermoid carcinoma cells or MDA-MB-453 cells were grown in 6-well plates to about 80% confluency and then incubated in serum-free media for 18 hours. The cells were exposed to various concentrations of either compound 1 or Example 21 for 2 hours and then stimulated with either 100 ng/mL
• EGF EGF
• MDA-MB-453 10 ng/mL heregulin
• Extracts were made by lysing the monolayers in 0.2 mL of boiling Laemlli buffer (2% sodium dodecyl sulfate, 5% beta-mercaptoethanol, 10% glycerol and
• the membrane was blotted for 2 hours with antiphosphotyrosine antibody (UBI, Lake Placid, NY, 1 ⁇ g/mL in blocking buffer) and then washed twice in TNA, once in TNA containing 0.05% Tween-20 and 0.05% nonidet P-40 and twice in TNA.
• the membranes were then incubated for 2 hours in blocking buffer containing 0.1 ⁇ Ci/mL of [125 JJ protein A and then washed again as above. After the blots were dry, they were loaded into a film cassette and exposed to X-AR x-ray film for 1 to 7 days. Band intensities were determined with a laser densitometer.
• Example 21 and compound 1 were about equally active against purified EGF receptor tyrosine kinase, EGF -mediated receptor autophosphorylation, and heregulin-mediated tyrosine phosphorylation in vitro. Table 1
• the human epidermoid carcinoma, A431 was propagated in vivo by serial transplantation. This tumor model was selected for these studies because of its known dependence upon the EGF receptor for growth and its early stage responsiveness to monoclonal antibody therapy in vivo.
• nude mice weighing 18-22 grams were implanted subcutaneously with 30 mg A431 tumor fragments in the region of the right axilla on Day 0. Tumors were allowed to grow to a mass of 100 to 150 mg, at which time the tumor bearing animals were randomized and distributed into treatment cages. Animals were treated perorally for 15 consecutive days with the isethionate salts of Example 21 and compound 1 in water.
• the isethionate salts can be prepared by titrating the compounds into solution with 2 equivalents of isethionic acid. Treatment was based on average group weight. The control animals received water. Evaluation of the experiment was based on tumor growth delay, T-C, defined as the difference, in days, for the treated and control tumors to reach an evaluation size of 750 mg. Data can also be represented as the percentage of tumor growth inhibition, defined as the tumor growth delay divided by the number of treatments times 100%. Larger tumor growth delays and tumor growth inhibition values are indicative of more active compounds. Statistical analysis was performed using JMP for
• the values for compound 1 are significantly different from those for Example 21 based on the time required for individual tumors to reach 750 mg. p ⁇ 0.05, student's t-test.
• VEGF Vascular endothelial growth factor
• NPF vascular permeability factor
• EGF epidermal growth factor
• transforming growth factor alpha both of which are ligands for EGF receptor (EGFR).
• VEGF secretion results in an antiangiogenic effect and can lead to tumor regression, since even partial inhibition of VEGF secretion can lead to the destruction of newly formed immature blood vessels in tumors.
• Boshel E. Conditional switching of VEGF expression in tumors: induction of endothelial cell shedding and regression of hemangioblastoma-like vessels by VEGF withdrawal. Proc. Natl Acad. Sci. USA, 1997;94:8761-8766).
• Example 21 inhibited VEGF secretion to almost the same level as observed with 0.1 ⁇ M of compound 1.
• 0.1 ⁇ M of the compound 1 inhibited EGF-stimulated VEGF secretion by 63% and 0.5 ⁇ M inhibited by 86%.
• 0.1 ⁇ M of Example 21 inhibited EGF- stimulated VEGF secretion by 48% and 0.5 ⁇ M of Example 21 inhibited EGF- stimulated VEGF secretion by 57%.
• Similar results were obtained using TGF- alpha to stimulate VEGF secretion in place of EGF.
• 0.5 ⁇ M of compound 1 inhibited TGF-alpha stimulated VEGF secretion by greater than 80% whereas the same concentration of Example 21 inhibited VEGF secretion by 57%.
• Compound 1 also appeared to inhibit the basal level of VEGF secretion from A431 cells whereas Example 21 had no effect.
• A431 human epidermoid carcinoma cells obtained from the American Type Culture Collection (Rockville, MD) were cultured in Dulbecco's MEM/F12 media (DMEM/F12) supplemented with 10% fetal bovine serum. Prior to drug and growth factor treatment, cells were washed three times in serum free DMEM F12. Cells were treated for 2 hours with the indicated concentration of compound lor Example 21 prior to the addition of either 10 ng/mL EGF or TGF- alpha. After 48 hours of incubation at 37°C, media was removed and stored frozen at -70°C. VEGF ELISA assays (Intergen, Co., Purchase, NY) are performed on media samples following the manufacturer's instructions. The results of this assay are shown below in tabular form. Treatment VEGF secretion Percent

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• Orthopedic Medicine & Surgery (AREA)
• Heart & Thoracic Surgery (AREA)
• Ophthalmology & Optometry (AREA)
• Pain & Pain Management (AREA)
• Cardiology (AREA)
• Vascular Medicine (AREA)
• Urology & Nephrology (AREA)
• Endocrinology (AREA)
• Reproductive Health (AREA)
• Dermatology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

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Cited By (71)

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Citations (1)

• 1999
  • 1999-09-23 EA EA200100546A patent/EA003786B1/ru not_active IP Right Cessation
  • 1999-09-23 CN CNB998146358A patent/CN1160338C/zh not_active Expired - Fee Related
  • 1999-09-23 AU AU62612/99A patent/AU763626B2/en not_active Ceased
  • 1999-09-23 KR KR1020017006276A patent/KR20010080491A/ko not_active Ceased
  • 1999-09-23 AT AT99949821T patent/ATE229008T1/de not_active IP Right Cessation
  • 1999-09-23 BR BR9915487-0A patent/BR9915487A/pt not_active IP Right Cessation
  • 1999-09-23 SK SK657-2001A patent/SK283688B6/sk unknown
  • 1999-09-23 EE EEP200100271A patent/EE200100271A/xx unknown
  • 1999-09-23 DK DK99949821T patent/DK1131304T3/da active
  • 1999-09-23 CA CA002349721A patent/CA2349721A1/en not_active Abandoned
  • 1999-09-23 JP JP2000583876A patent/JP3687900B2/ja not_active Expired - Fee Related
  • 1999-09-23 NZ NZ527718A patent/NZ527718A/en unknown
  • 1999-09-23 CZ CZ20011759A patent/CZ20011759A3/cs unknown
  • 1999-09-23 PT PT99949821T patent/PT1131304E/pt unknown
  • 1999-09-23 DE DE69904358T patent/DE69904358T2/de not_active Expired - Fee Related
  • 1999-09-23 WO PCT/US1999/022116 patent/WO2000031048A1/en not_active Ceased
  • 1999-09-23 EP EP99949821A patent/EP1131304B1/en not_active Expired - Lifetime
  • 1999-09-23 HU HU0104211A patent/HUP0104211A3/hu unknown
  • 1999-09-23 ES ES99949821T patent/ES2188254T3/es not_active Expired - Lifetime
  • 1999-09-23 PL PL99347717A patent/PL347717A1/xx not_active IP Right Cessation
  • 1999-09-23 IL IL14308999A patent/IL143089A0/xx unknown
  • 1999-09-23 GE GEAP19995950A patent/GEP20032997B/en unknown
  • 1999-09-23 US US09/831,991 patent/US6344455B1/en not_active Expired - Fee Related
  • 1999-09-23 NZ NZ512189A patent/NZ512189A/en unknown
  • 1999-11-18 PE PE1999001167A patent/PE20001242A1/es not_active Application Discontinuation
  • 1999-11-18 UY UY25809A patent/UY25809A1/es unknown
  • 1999-11-18 AR ARP990105858A patent/AR023706A1/es unknown
  • 1999-11-18 CO CO99072597A patent/CO5150185A1/es unknown
• 2001
  • 2001-05-02 ZA ZA200103535A patent/ZA200103535B/en unknown
  • 2001-05-18 NO NO20012465A patent/NO319357B1/no unknown
  • 2001-06-15 BG BG105608A patent/BG105608A/xx unknown

Patent Citations (1)

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