WO1998000231A1 - High-throughput screening assay systems in microscale fluidic devices - Google Patents

High-throughput screening assay systems in microscale fluidic devices Download PDF

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Publication number
WO1998000231A1
WO1998000231A1 PCT/US1997/010894 US9710894W WO9800231A1 WO 1998000231 A1 WO1998000231 A1 WO 1998000231A1 US 9710894 W US9710894 W US 9710894W WO 9800231 A1 WO9800231 A1 WO 9800231A1
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WO
WIPO (PCT)
Prior art keywords
channels
channel
component
biochemical system
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1997/010894
Other languages
English (en)
French (fr)
Other versions
WO1998000231A9 (en
Inventor
John Wallace Parce
Anne R. Kopf-Sill
Luc J. Bousse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Technologies Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/671,987 external-priority patent/US5942443A/en
Priority to DE69738941T priority Critical patent/DE69738941D1/de
Priority to BR9710054-4A priority patent/BR9710054A/pt
Priority to NZ333346A priority patent/NZ333346A/xx
Priority to CA002258489A priority patent/CA2258489C/en
Priority to US09/214,035 priority patent/US6429025B1/en
Application filed by Caliper Technologies Corp filed Critical Caliper Technologies Corp
Priority to EP97931337A priority patent/EP0907412B1/en
Priority to JP50423798A priority patent/JP3788519B2/ja
Priority to AU34990/97A priority patent/AU729537B2/en
Publication of WO1998000231A1 publication Critical patent/WO1998000231A1/en
Publication of WO1998000231A9 publication Critical patent/WO1998000231A9/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • G—PHYSICS
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    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S366/02—Micromixers: segmented laminar flow with boundary mixing orthogonal to the direction of fluid propagation with or without geometry influences from the pathway
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    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Definitions

  • test compound refers to the collection of compounds that are to be screened for their ability to affect a particular biochemical system.
  • Test compounds may include a wide variety of different compounds, including chemical compounds, mixtures of chemical compounds, e.g., polysaccharides, small organic or inorganic molecules, biological macromolecules, e.g., peptides, proteins, nucleic acids, or an extract made from biological materials such as bacteria, plants, fungi, or animal cells or tissues, naturally occurring or synthetic compositions.
  • the test compounds are provided, e.g., injected, free in solution, or are optionally attached to a carrier, or a solid support, e.g., beads.
  • electroosmotic fluid direction systems examples include, e.g., those described in International Patent Application No. WO 96/04547 to Ramsey et al. , which is inco ⁇ orated herein by reference in its entirety for all pu ⁇ oses.
  • Inco ⁇ orating this electroosmotic fluid direction system into the device shown in Figure 1 involves inco ⁇ oration of an electrode within each of the reservoirs 104, 106 and 108, and at the terminus of sample channel 112 or at the terminus of any fluid channels connected thereto, whereby the electrode is in electrical contact with the fluid disposed in the respective reservoir or channel.
  • Action of the enzyme upon the substrate will produce an increasing level of the fluorescent or chromatic signal. This increasing signal is indicated by the increasing shading within the main channel as it approaches the detection window. This signal trend will also occur within those test compound or subject material regions which have no effect on the enzyme/substrate interaction, e.g., test compound 126. Where a test compound does have an effect on the interaction of the enzyme and the substrate, a variation will appear in the signal produced.
  • microfluidic system containing at least a first substrate having a first channel and a second channel intersecting said first channel, at least one of said channels having at least one cross-sectional dimension in a range from
  • Apparatus as hereinbefore described having at least two transverse channels fabricated into the surface, wherein each of the plurality of reaction channels is fluidly connected to a first of the at least two transverse channels at a first point in each of the reaction channels, and fluidly connected to a second transverse channel at a second point in each of the reaction channels and an assay apparatus including an apparatus as hereinbefore described are also provided. Examples
  • Enzyme and substrate were continually pumped irough the main channel from their respective ports under both voltage states. Inhibitor or Buffer were delivered into the main channel alternately from their respective wells by alternating between voltage state 1 and voltage state 2. When no inhibitor was present at the detection end of the main channel, a base line level of fluorescent product was produced. Upon introduction of inhibitor, the fluorescent signal was greatly reduced, indicating inhibition of the enzyme/substrate interaction. Fluorescent data obtained from the alternating delivery of inhibitor and buffer into the main channel is shown in Figure 9A.
  • Figure 9B a supe ⁇ osition of the two data segments from Figure 9A, directly comparing the inhibitor data widi control (buffer) data. The control shows only a minor fluctuation in the fluorescent signal that apparently resulted from a dilution of the enzyme substrate mixture, whereas the inhibitor screen shows a substantial reduction in the fluorescent signal, indicating clear inhibition.
  • FIG. 11 A schematic of timing for subject material region spacer region loading is shown in Figure 11.
  • the top panel shows the subject material/first spacer region/second spacer region distribution widiin a channel, whereas the lower panel shows the timing required for loading the channel.
  • the schematic includes die loading (sipping) of high salt (HS) first spacer fluid ("A"), moving die pipettor to the sample or subject material ("B"), sipping the sample or subject material ("C"), moving the pipettor to the high salt first spacer fluid ("D”) sipping the first spacer fluid ("E"), moving the pipettor to the low salt (LS) or second spacer fluid (“F”), sipping the second spacer fluid ("G”) and returning to the first spacer fluid (“H”).
  • HS high salt
  • B moving die pipettor to the sample or subject material
  • C sipping the sample or subject material
  • D moving the pipettor to the high salt first spacer fluid
  • E sipping the first spacer fluid
  • LS low salt
  • F

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PCT/US1997/010894 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices Ceased WO1998000231A1 (en)

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AU34990/97A AU729537B2 (en) 1996-06-28 1997-06-24 High throughput screening assay systems in microscale fluidic devices
BR9710054-4A BR9710054A (pt) 1996-06-28 1997-06-24 Aparelhos para separar compostos de teste para um efeito sobre um sistema bioquìmico e para detectar ummefeito de um composto de teste sobre um sistema bioquìmico, processos de determinação de se uma amostra contém um composto capaz de afetar um sistema bioquìmico, de separação de uma pluralidade de compostos de teste para um efeito sobre um sistema bioquìmico e usos de um sistema microfluido e de um substrato de ensaio.
NZ333346A NZ333346A (en) 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices
CA002258489A CA2258489C (en) 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices
US09/214,035 US6429025B1 (en) 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices
DE69738941T DE69738941D1 (de) 1996-06-28 1997-06-24 Hochdurchsatz-screening-testsystem mittels mikrofluidischer vorrichtungen
EP97931337A EP0907412B1 (en) 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices
JP50423798A JP3788519B2 (ja) 1996-06-28 1997-06-24 微小スケール流体装置の高処理能力スクリーニングアッセイシステム

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US08/671,987 1996-06-28
US08/671,987 US5942443A (en) 1996-06-28 1996-06-28 High throughput screening assay systems in microscale fluidic devices
US08/761,575 1996-12-06
US08/761,575 US6046056A (en) 1996-06-28 1996-12-06 High throughput screening assay systems in microscale fluidic devices

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Cited By (215)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001231A (en) * 1997-07-15 1999-12-14 Caliper Technologies Corp. Methods and systems for monitoring and controlling fluid flow rates in microfluidic systems
WO1999064836A1 (en) * 1998-06-08 1999-12-16 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
WO1999065664A1 (en) * 1998-06-18 1999-12-23 3M Innovative Properties Company Microfluidic articles and method of manufacturing same
US6007690A (en) * 1996-07-30 1999-12-28 Aclara Biosciences, Inc. Integrated microfluidic devices
WO2000022428A1 (en) * 1998-10-13 2000-04-20 Astrazeneca Ab Device
WO2000022424A1 (en) * 1998-10-14 2000-04-20 Caliper Technologies Corp. Microfluidic controller and detector system with self-calibration
US6068752A (en) * 1997-04-25 2000-05-30 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
EP0975474A4 (en) * 1997-04-14 2000-08-02 Caliper Techn Corp CONTROLLED LIQUID TRANSPORT IN POLYMER SUBSTRATES THROUGH MICROCHANNELS
DE19910392A1 (de) * 1999-03-05 2000-09-07 Inst Physikalische Hochtech Ev Mikrosäulenreaktor
WO2000062050A1 (en) * 1999-04-09 2000-10-19 The University Of Manchester Institute Of Science & Technology Electrophoresis method and apparatus with time- or space-modulated sample injection
WO2000070080A1 (en) 1999-05-17 2000-11-23 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
WO2000077507A1 (de) * 1999-06-16 2000-12-21 Merck Patent Gmbh Vorrichtung zur probenaufgabe
WO2000078453A1 (fr) * 1999-06-22 2000-12-28 Biomerieux S.A. Vannes permettant de diriger un fluide dans une carte d'analyse, cartes d'analyse et dispositif d'analyse comprenant plusieurs cartes
JP2001004628A (ja) * 1999-06-18 2001-01-12 Kanagawa Acad Of Sci & Technol 免疫分析装置と免疫分析方法
WO2001012327A1 (en) * 1999-08-12 2001-02-22 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
DE19935433A1 (de) * 1999-08-01 2001-03-01 Febit Ferrarius Biotech Gmbh Mikrofluidischer Reaktionsträger
WO2000070353A3 (en) * 1999-05-13 2001-04-26 Caliper Techn Corp High throughput assay systems and methods
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6271038B1 (en) 1999-12-15 2001-08-07 Glaxo Wellcome Inc. Methods for high throughout determination and ranking of formulations and solubility
US6274089B1 (en) 1998-06-08 2001-08-14 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
US6287774B1 (en) 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US6306659B1 (en) 1996-06-28 2001-10-23 Caliper Technologies Corp. High throughput screening assay systems in microscale fluidic devices
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
JP2001340753A (ja) * 2000-03-29 2001-12-11 Sumitomo Chem Co Ltd 反応方法および反応装置
WO1999061888A3 (en) * 1998-05-22 2001-12-13 California Inst Of Techn Microfabricated cell sorter
US6379884B2 (en) 2000-01-06 2002-04-30 Caliper Technologies Corp. Methods and systems for monitoring intracellular binding reactions
US6381846B2 (en) 1998-06-18 2002-05-07 3M Innovative Properties Company Microchanneled active fluid heat exchanger method
EP1088229A4 (en) * 1998-06-25 2002-05-08 Caliper Techn Corp HIGH THROUGHPUT METHODS, SYSTEMS AND APPARATUS FOR CELL SCREENING TEST
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6403338B1 (en) 1997-04-04 2002-06-11 Mountain View Microfluidic systems and methods of genotyping
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US6431695B1 (en) 1998-06-18 2002-08-13 3M Innovative Properties Company Microstructure liquid dispenser
EP1163052A4 (en) * 1999-02-23 2002-08-14 Caliper Techn Corp MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
US6436646B1 (en) 1999-05-21 2002-08-20 Caliper Technologies Corp. Methods of identifying nucleic acid sequences using polycations
EP1063204A3 (en) * 1999-06-21 2002-08-21 Micro Chemical Systems Limited Chemical devices, methods of manufacturing and of using chemical devices
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6447661B1 (en) 1998-10-14 2002-09-10 Caliper Technologies Corp. External material accession systems and methods
US6458259B1 (en) 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
US6468763B1 (en) 1999-09-29 2002-10-22 Caliper Technologies Corp. Optical detection of transmembrane potential changes
US6468761B2 (en) 2000-01-07 2002-10-22 Caliper Technologies, Corp. Microfluidic in-line labeling method for continuous-flow protease inhibition analysis
US6495369B1 (en) 1998-08-10 2002-12-17 Caliper Technologies Corp. High throughput microfluidic systems and methods
EP1185871A4 (en) * 1999-06-01 2003-01-15 Caliper Techn Corp MICRO-SCALE ASSAYS AND MICROFLUIDIC DEVICES FOR THE CONTROL OF TRANSPORTER, INDUCED GRADIENT AND BINDING ACTIVITIES
US6514412B1 (en) 1998-06-18 2003-02-04 3M Innovative Properties Company Microstructured separation device
US6524790B1 (en) 1997-06-09 2003-02-25 Caliper Technologies Corp. Apparatus and methods for correcting for variable velocity in microfluidic systems
US6537771B1 (en) 1999-10-08 2003-03-25 Caliper Technologies Corp. Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6542858B1 (en) 1998-09-14 2003-04-01 Lion Bioscience Ag Pharmacokinetic-based drug design tool and method
US6540896B1 (en) 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
US6556923B2 (en) 2000-01-26 2003-04-29 Caliper Technologies Corp. Software for high throughput microfluidic systems
US6558944B1 (en) 1996-06-28 2003-05-06 Caliper Technologies Corp. High throughput screening assay systems in microscale fluidic devices
AU760310B2 (en) * 1997-04-14 2003-05-15 Caliper Life Sciences, Inc. Controlled fluid transport in microfabricated polymeric substrates
US6569607B2 (en) 2000-05-03 2003-05-27 Caliper Technologies Corp. Multi depth substrate fabrication processes
US6589729B2 (en) 2000-02-04 2003-07-08 Caliper Technologies Corp. Methods, devices, and systems for monitoring time dependent reactions
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US6613211B1 (en) 1999-08-27 2003-09-02 Aclara Biosciences, Inc. Capillary electrokinesis based cellular assays
US6635487B1 (en) 2000-05-17 2003-10-21 Caliper Technologies Corp. Fluorescence standard for use in microfluidic instruments
AU768135B2 (en) * 1999-08-12 2003-12-04 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US6669831B2 (en) 2000-05-11 2003-12-30 Caliper Technologies Corp. Microfluidic devices and methods to regulate hydrodynamic and electrical resistance utilizing bulk viscosity enhancers
JP2004508837A (ja) * 2000-09-14 2004-03-25 カリパー・テクノロジーズ・コープ． 温度介在反応を行うためのマイクロ流体装置及び方法
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6733645B1 (en) 2000-04-18 2004-05-11 Caliper Technologies Corp. Total analyte quantitation
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6777184B2 (en) 2000-05-12 2004-08-17 Caliper Life Sciences, Inc. Detection of nucleic acid hybridization by fluorescence polarization
US6827831B1 (en) 1997-08-29 2004-12-07 Callper Life Sciences, Inc. Controller/detector interfaces for microfluidic systems
DE10320869A1 (de) * 2003-05-09 2004-12-16 Evotec Technologies Gmbh Verfahren und Vorrichtungen zur Flüssigkeitsbehandlung suspendierter Partikel
US6846668B1 (en) 1998-10-08 2005-01-25 Astrazeneca Ab Microfabricated cell injector
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6869799B1 (en) 1998-06-09 2005-03-22 Symyx Technologies, Inc. Methods for screening catalysts in a parallel fixed-bed reactor
WO2005062059A1 (en) * 2003-12-22 2005-07-07 Digital Bio Technology Channel apparatus for focusing a fluid flow
EP1144674A4 (en) * 1999-01-19 2005-07-27 Caliper Life Sciences Inc HIGH PERFORMANCE OPTIMIZED ANALYTICAL SYSTEM
US6929730B2 (en) * 2001-05-01 2005-08-16 Cheng Sheng Lee Two dimensional microfluidic gene scanner
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
AU2003204460B2 (en) * 1998-10-14 2005-11-10 Caliper Life Sciences, Inc. Microfluidic controller and detector system with self-calibration
US6974526B2 (en) * 2001-05-01 2005-12-13 Calibrant Biosystems, Inc. Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
US7033474B1 (en) 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US7037416B2 (en) 2000-01-14 2006-05-02 Caliper Life Sciences, Inc. Method for monitoring flow rate using fluorescent markers
US7070682B2 (en) 2001-01-16 2006-07-04 Cheng Lee Microfluidic apparatus for performing gel protein extractions and methods for using the apparatus
KR100597870B1 (ko) * 2004-04-01 2006-07-06 한국과학기술원 고속 스크리닝 또는 고속 분석을 위한 미소 유체 칩
US7118917B2 (en) 2001-03-07 2006-10-10 Symyx Technologies, Inc. Parallel flow reactor having improved thermal control
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7122156B2 (en) 2000-03-07 2006-10-17 Symyx Technologies, Inc. Parallel flow reactor having variable composition
US7150994B2 (en) 1999-03-03 2006-12-19 Symyx Technologies, Inc. Parallel flow process optimization reactor
US7155344B1 (en) 1998-07-27 2006-12-26 Caliper Life Sciences, Inc. Distributed database for analytical instruments
US7160423B2 (en) 2002-03-05 2007-01-09 Caliper Life Sciences, Inc. Mixed mode microfluidic systems
US7192559B2 (en) 2000-08-03 2007-03-20 Caliper Life Sciences, Inc. Methods and devices for high throughput fluid delivery
US7223364B1 (en) 1999-07-07 2007-05-29 3M Innovative Properties Company Detection article having fluid control film
US7252928B1 (en) 2002-03-12 2007-08-07 Caliper Life Sciences, Inc. Methods for prevention of surface adsorption of biological materials to capillary walls in microchannels
US7276330B2 (en) 1999-01-28 2007-10-02 Caliper Technologies Corp. Devices, systems and methods for time domain multiplexing of reagents
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
EP1902779A2 (en) 2002-02-25 2008-03-26 Hitachi Chemical Co., Ltd. A support unit for a microfluidic system
US7378280B2 (en) 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
WO2008097559A3 (en) * 2007-02-06 2008-10-09 Univ Brandeis Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) * 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US7452726B2 (en) 2002-04-01 2008-11-18 Fluidigm Corporation Microfluidic particle-analysis systems
US7497994B2 (en) 1998-02-24 2009-03-03 Khushroo Gandhi Microfluidic devices and systems incorporating cover layers
US7521186B2 (en) 2000-03-20 2009-04-21 Caliper Lifesciences Inc. PCR compatible nucleic acid sieving matrix
EP2092989A1 (en) 1998-05-06 2009-08-26 Caliper Life Sciences, Inc. Methods of fabricating polymeric structures incorporating microscale fluidic elements
US7582461B2 (en) 2003-07-29 2009-09-01 Life Technologies Corporation Kinase and phosphatase assays
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7641780B2 (en) 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
US7645581B2 (en) 2002-12-20 2010-01-12 Caliper Life Sciences, Inc. Determining nucleic acid fragmentation status by coincident detection of two labeled probes
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
EP2259053A2 (en) 1997-04-25 2010-12-08 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US8043581B2 (en) 2001-09-12 2011-10-25 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8216852B2 (en) 2001-07-27 2012-07-10 Caliper Life Sciences, Inc. Channel cross-section geometry to manipulate dispersion rates
US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
EP2482075A1 (en) 2004-12-01 2012-08-01 Proteologics, Ltd Ubiquitin ligase assays and related reagents
US8275554B2 (en) 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US8304230B2 (en) 2002-09-27 2012-11-06 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US8480970B2 (en) 2004-11-30 2013-07-09 Hitachi Chemical Co., Ltd. Analytical pretreatment device
EP2352025A4 (en) * 2008-08-11 2013-08-07 Fujimori Kogyo Co Ltd THROMBOZY TEST METHOD AND THROMBOZYTENTESTVORRICHTUNG
US8658418B2 (en) 2002-04-01 2014-02-25 Fluidigm Corporation Microfluidic particle-analysis systems
US8796031B2 (en) 2010-02-10 2014-08-05 Fujimori Kogyo Co., Ltd. Microchip for platelet examination and platelet examination device using same
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US8871444B2 (en) 2004-10-08 2014-10-28 Medical Research Council In vitro evolution in microfluidic systems
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9040288B2 (en) 2006-03-24 2015-05-26 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US9080207B2 (en) 2006-03-24 2015-07-14 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9089844B2 (en) 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US9126160B2 (en) 2008-09-23 2015-09-08 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US9211537B2 (en) 2007-11-07 2015-12-15 The University Of British Columbia Microfluidic device and method of using same
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US9222128B2 (en) 2011-03-18 2015-12-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US9273308B2 (en) 2006-05-11 2016-03-01 Raindance Technologies, Inc. Selection of compartmentalized screening method
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US9328344B2 (en) 2006-01-11 2016-05-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9393560B2 (en) 2010-03-25 2016-07-19 Bio-Rad Laboratories, Inc. Droplet transport system for detection
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9415392B2 (en) 2009-03-24 2016-08-16 The University Of Chicago Slip chip device and methods
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9448172B2 (en) 2003-03-31 2016-09-20 Medical Research Council Selection by compartmentalised screening
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9498759B2 (en) 2004-10-12 2016-11-22 President And Fellows Of Harvard College Compartmentalized screening by microfluidic control
US9500664B2 (en) 2010-03-25 2016-11-22 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US9664619B2 (en) 2008-04-28 2017-05-30 President And Fellows Of Harvard College Microfluidic device for storage and well-defined arrangement of droplets
US9670528B2 (en) 2003-07-31 2017-06-06 Handylab, Inc. Processing particle-containing samples
US9714443B2 (en) 2002-09-25 2017-07-25 California Institute Of Technology Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors
US9765389B2 (en) 2011-04-15 2017-09-19 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US9815057B2 (en) 2006-11-14 2017-11-14 Handylab, Inc. Microfluidic cartridge and method of making same
US9839890B2 (en) 2004-03-31 2017-12-12 National Science Foundation Compartmentalised combinatorial chemistry by microfluidic control
EP3201596A4 (en) * 2014-10-01 2018-07-11 University of Tasmania Extraction and concentration device
US10052605B2 (en) 2003-03-31 2018-08-21 Medical Research Council Method of synthesis and testing of combinatorial libraries using microcapsules
US10067123B2 (en) 2006-10-13 2018-09-04 Theranos Ip Company Llc Reducing optical interference in a fluidic device
US10131934B2 (en) 2003-04-03 2018-11-20 Fluidigm Corporation Thermal reaction device and method for using the same
US10179910B2 (en) 2007-07-13 2019-01-15 Handylab, Inc. Rack for sample tubes and reagent holders
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US10428377B2 (en) 2002-12-20 2019-10-01 Caliper Life Sciences, Inc. Methods of detecting low copy nucleic acids
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
US10578633B2 (en) 2013-03-15 2020-03-03 Fluidigm Corporation Methods and devices for analysis of defined multicellular combinations
US10620194B2 (en) 2001-03-19 2020-04-14 Gyros Patent Ab Characterization of reaction variables
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
EP3677336A1 (en) 2007-09-05 2020-07-08 Caliper Life Sciences Inc. Microfluidic method and system for enzyme inhibition activity screening
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10994273B2 (en) 2004-12-03 2021-05-04 Cytonome/St, Llc Actuation of parallel microfluidic arrays
US11027278B2 (en) 2002-04-17 2021-06-08 Cytonome/St, Llc Methods for controlling fluid flow in a microfluidic system
US11077415B2 (en) 2011-02-11 2021-08-03 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US11174509B2 (en) 2013-12-12 2021-11-16 Bio-Rad Laboratories, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
US11268887B2 (en) 2009-03-23 2022-03-08 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
US11378498B2 (en) 2006-06-14 2022-07-05 Verinata Health, Inc. Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US11390917B2 (en) 2010-02-12 2022-07-19 Bio-Rad Laboratories, Inc. Digital analyte analysis
US11453906B2 (en) 2011-11-04 2022-09-27 Handylab, Inc. Multiplexed diagnostic detection apparatus and methods
US11511242B2 (en) 2008-07-18 2022-11-29 Bio-Rad Laboratories, Inc. Droplet libraries
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11898193B2 (en) 2011-07-20 2024-02-13 Bio-Rad Laboratories, Inc. Manipulating droplet size
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
US12097475B2 (en) 2004-07-02 2024-09-24 The University Of Chicago Microfluidic system
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US12168231B2 (en) 2008-09-23 2024-12-17 Bio-Rad Laboratories, Inc. Method of analysis

Families Citing this family (482)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6048734A (en) 1995-09-15 2000-04-11 The Regents Of The University Of Michigan Thermal microvalves in a fluid flow method
WO1997040385A1 (en) 1996-04-25 1997-10-30 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6547942B1 (en) * 1996-06-28 2003-04-15 Caliper Technologies Corp. Electropipettor and compensation means for electrophoretic bias
CA2286590C (en) * 1997-04-18 2005-08-02 California Institute Of Technology Multifunctional polymeric tissue coatings
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
DE19815882A1 (de) * 1998-04-08 1999-10-14 Fuhr Guenther Verfahren und Vorrichtung zur Manipulierung von Mikropartikeln in Fluidströmungen
US6787017B1 (en) * 1998-10-09 2004-09-07 Hitachi, Ltd. Capillary electrophoretis system, sample analyzer and liquid sample cassette for electrophoretic separation
NZ511560A (en) * 1998-11-05 2002-11-26 Chemometec As A method for the assessment of particles and a system and a device for use in the method
JP3441993B2 (ja) * 1999-01-27 2003-09-02 松下電器産業株式会社 コレステロールセンサ
US6322683B1 (en) * 1999-04-14 2001-11-27 Caliper Technologies Corp. Alignment of multicomponent microfabricated structures
WO2000065352A1 (en) * 1999-04-28 2000-11-02 Eidgenossisch Technische Hochschule Zurich Polyionic coatings in analytic and sensor devices
JP3417344B2 (ja) 1999-05-26 2003-06-16 株式会社島津製作所 化学発光検出方法及びその方法を用いたマイクロチップ電気泳動装置
DE19928412C2 (de) * 1999-06-22 2002-03-21 Agilent Technologies Inc Versorgungselement für einen Labor-Mikrochip
US7195670B2 (en) 2000-06-27 2007-03-27 California Institute Of Technology High throughput screening of crystallization of materials
US7052545B2 (en) * 2001-04-06 2006-05-30 California Institute Of Technology High throughput screening of crystallization of materials
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
US7138254B2 (en) * 1999-08-02 2006-11-21 Ge Healthcare (Sv) Corp. Methods and apparatus for performing submicroliter reactions with nucleic acids or proteins
DE19949551C2 (de) * 1999-10-14 2001-12-13 Agilent Technologies Inc Mikrofluidischer Mikrochip, Energieversorgungseinrichtung und Verfahren zum Betrieb eines mikrofluidischen Mikrochips
US6908594B1 (en) * 1999-10-22 2005-06-21 Aclara Biosciences, Inc. Efficient microfluidic sealing
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2290731A1 (en) 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6620625B2 (en) * 2000-01-06 2003-09-16 Caliper Technologies Corp. Ultra high throughput sampling and analysis systems and methods
EP1123739B1 (en) * 2000-02-11 2006-11-29 STMicroelectronics S.r.l. Integrated device for microfluid thermoregulation, and manufacturing process thereof
CA2399398A1 (en) * 2000-02-11 2001-08-16 Aclara Biosciences, Inc. Microfluid device with sample injector and method of use
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US7332271B2 (en) * 2000-02-18 2008-02-19 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US6681616B2 (en) 2000-02-23 2004-01-27 Caliper Technologies Corp. Microfluidic viscometer
AU4907101A (en) 2000-02-23 2001-09-03 Caliper Technologies Inc Multi-reservoir pressure control system
US7040144B2 (en) * 2000-02-23 2006-05-09 Caliper Life Sciences, Inc. Microfluidic viscometer
US7732192B2 (en) * 2000-02-29 2010-06-08 Stmicroelectronics S.R.L. Integrated chemical microreactor with large area channels and manufacturing process thereof
US7452713B2 (en) * 2000-02-29 2008-11-18 Stmicroelectronics S.R.L. Process for manufacturing a microfluidic device with buried channels
US7230315B2 (en) * 2000-02-29 2007-06-12 Stmicroelectronics S.R.L. Integrated chemical microreactor with large area channels and manufacturing process thereof
US7485454B1 (en) * 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
JP4648513B2 (ja) * 2000-03-15 2011-03-09 関東化学株式会社 濃度検知方法及び濃度検知装置並びに薬剤の希釈調合装置
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
US8105553B2 (en) * 2004-01-25 2012-01-31 Fluidigm Corporation Crystal forming devices and systems and methods for using the same
US6436722B1 (en) * 2000-04-18 2002-08-20 Idexx Laboratories, Inc. Device and method for integrated diagnostics with multiple independent flow paths
WO2001093930A1 (en) * 2000-06-02 2001-12-13 The University Of Utah Research Foundation Active needle devices with integrated functionality
EP1161985B1 (en) * 2000-06-05 2005-10-26 STMicroelectronics S.r.l. Process for manufacturing integrated chemical microreactors of semiconductor material, and integrated microreactor
WO2001098785A2 (en) * 2000-06-19 2001-12-27 Arizona Board Of Regents Rapid flow-based immunoassay microchip
JP4744778B2 (ja) 2000-06-21 2011-08-10 バイオアレイ ソルーションズ リミテッド 特定のランダム粒子アレイを適用した複数の被検体分子の分析方法
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
US6885982B2 (en) 2000-06-27 2005-04-26 Fluidigm Corporation Object oriented microfluidic design method and system
AU2001273057A1 (en) * 2000-06-27 2002-01-08 Fluidigm Corporation A microfluidic design automation method and system
US6627159B1 (en) * 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
JP2002022752A (ja) * 2000-07-13 2002-01-23 Suzuki Motor Corp 検体試験装置
DE60032772T2 (de) * 2000-09-27 2007-11-08 Stmicroelectronics S.R.L., Agrate Brianza Integrierter chemischer Mikroreaktor mit thermisch isolierten Messelektroden und Verfahren zu dessen Herstellung
US20100261159A1 (en) 2000-10-10 2010-10-14 Robert Hess Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
CA2425476C (en) * 2000-10-10 2011-02-01 Biotrove, Inc. Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
WO2002030486A2 (en) 2000-10-13 2002-04-18 Fluidigm Corporation Microfluidic device based sample injection system for analytical devices
US20050221385A1 (en) * 2000-11-07 2005-10-06 Caliper Life Sciences, Inc. Pressure based mobility shift assays
US7105304B1 (en) 2000-11-07 2006-09-12 Caliper Life Sciences, Inc. Pressure-based mobility shift assays
US8097471B2 (en) * 2000-11-10 2012-01-17 3M Innovative Properties Company Sample processing devices
CA2697026A1 (en) * 2000-12-12 2002-06-12 Bayer Healthcare Llc Method of making a capillary channel
US20020108860A1 (en) * 2001-01-15 2002-08-15 Staats Sau Lan Tang Fabrication of polymeric microfluidic devices
US6875403B2 (en) * 2001-02-09 2005-04-05 Microchem Solutions Method and apparatus for reproducible sample injection on microfabricated devices
US20020166592A1 (en) * 2001-02-09 2002-11-14 Shaorong Liu Apparatus and method for small-volume fluid manipulation and transportation
US6913697B2 (en) * 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
EP1360472B1 (en) * 2001-02-15 2009-12-02 Caliper Life Sciences, Inc. Microfluidic systems with enhanced detection systems
US7016560B2 (en) * 2001-02-28 2006-03-21 Lightwave Microsystems Corporation Microfluidic control for waveguide optical switches, variable attenuators, and other optical devices
WO2002068821A2 (en) 2001-02-28 2002-09-06 Lightwave Microsystems Corporation Microfluidic control using dieletric pumping
US6949377B2 (en) * 2001-03-05 2005-09-27 Ho Winston Z Chemiluminescence-based microfluidic biochip
US20020127740A1 (en) * 2001-03-06 2002-09-12 Ho Winston Z. Quantitative microfluidic biochip and method of use
US7223363B2 (en) * 2001-03-09 2007-05-29 Biomicro Systems, Inc. Method and system for microfluidic interfacing to arrays
US7323140B2 (en) 2001-03-28 2008-01-29 Handylab, Inc. Moving microdroplets in a microfluidic device
US7314718B1 (en) 2001-04-03 2008-01-01 Bioarray Solutions Ltd. Method and apparatus for maintaining multiple planar fluid flows
AU2002303311B2 (en) * 2001-04-10 2007-01-25 Bioprocessors Corporation Microfermentor device and cell based screening method
CA2444836C (en) * 2001-04-19 2012-03-27 Adhesives Research, Inc. Hydrophilic diagnostic devices for use in the assaying of biological fluids
US7476533B2 (en) * 2002-04-19 2009-01-13 Adhesives Research, Inc. Diagnostic devices for use in the assaying of biological fluids
US6727479B2 (en) * 2001-04-23 2004-04-27 Stmicroelectronics S.R.L. Integrated device based upon semiconductor technology, in particular chemical microreactor
JP4566456B2 (ja) * 2001-05-31 2010-10-20 独立行政法人理化学研究所 微量液体制御機構および微量液体制御方法
US20030077570A1 (en) * 2001-09-20 2003-04-24 Coventor, Inc. Small molecule substrate based enzyme activity assays
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20050149304A1 (en) * 2001-06-27 2005-07-07 Fluidigm Corporation Object oriented microfluidic design method and system
AU2002367839A1 (en) 2001-07-16 2003-11-17 Hk Pharmaceuticals, Inc. Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
US7019831B2 (en) * 2001-08-24 2006-03-28 Applera Corporation Separation device substrate including non-fluorescent quencher dye
US7666661B2 (en) 2001-08-27 2010-02-23 Platypus Technologies, Llc Substrates, devices, and methods for quantitative liquid crystal assays
WO2003025115A1 (en) * 2001-09-20 2003-03-27 Cytonome, Inc. Small molecule substrate based enzyme activity assays
ES2712173T3 (es) 2001-10-15 2019-05-09 Bioarray Solutions Ltd Análisis multiplexado de loci polimórficos mediante consulta simultánea y detección mediada por enzimas
EP1304167B1 (en) * 2001-10-18 2004-07-28 Aida Engineering Ltd. Micro-globule metering and sampling structure and microchips having the structure
US20030082632A1 (en) * 2001-10-25 2003-05-01 Cytoprint, Inc. Assay method and apparatus
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
US6882500B2 (en) * 2001-10-26 2005-04-19 Seagate Technology Llc Airflow channel within a disc drive housing
US20030085952A1 (en) * 2001-11-05 2003-05-08 Williams Roger O Apparatus and method for controlling the free surface of liquid in a well plate
SE526185C2 (sv) 2001-11-07 2005-07-19 Prolight Diagnostics Ab Metod och anordning för immunanalys
WO2003042697A1 (en) * 2001-11-14 2003-05-22 Genospectra, Inc. Biochemical analysis system with combinatorial chemistry applications
US20030098661A1 (en) * 2001-11-29 2003-05-29 Ken Stewart-Smith Control system for vehicle seats
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
US20060078961A1 (en) * 2002-02-12 2006-04-13 Daniel Chiu Systems and methods for rapidly changing the solution environment around sensors
US7470518B2 (en) * 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
US6814859B2 (en) * 2002-02-13 2004-11-09 Nanostream, Inc. Frit material and bonding method for microfluidic separation devices
US7419821B2 (en) * 2002-03-05 2008-09-02 I-Stat Corporation Apparatus and methods for analyte measurement and immunoassay
WO2003077851A2 (en) * 2002-03-11 2003-09-25 Hk Pharmaceuticals, Inc. Compounds and methods for analyzing the proteome
WO2003104439A2 (en) * 2002-03-12 2003-12-18 Surface Logix, Inc. Assay device that analyzes the absorption, metabolism, permeability and/or toxicity of a candidate compound
US7524462B2 (en) * 2002-03-29 2009-04-28 Agilent Technologies, Inc. Capillary flow for a heterogenous assay in a micro-channel environment
WO2003092618A2 (en) 2002-04-30 2003-11-13 University Of South Florida Materials and methods for prevention and treatment of rna viral diseases
US7129091B2 (en) * 2002-05-09 2006-10-31 University Of Chicago Device and method for pressure-driven plug transport and reaction
US20030208936A1 (en) * 2002-05-09 2003-11-13 Lee Charles Hee Method for manufacturing embroidery decorated cards and envelopes
AU2003299522B2 (en) * 2002-05-22 2008-03-06 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US20030228566A1 (en) * 2002-06-11 2003-12-11 Biotechplex Corporation Method of and apparatus for screening for drug candidates
JP2006507921A (ja) * 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US7364647B2 (en) * 2002-07-17 2008-04-29 Eksigent Technologies Llc Laminated flow device
US7517440B2 (en) 2002-07-17 2009-04-14 Eksigent Technologies Llc Electrokinetic delivery systems, devices and methods
US7235164B2 (en) * 2002-10-18 2007-06-26 Eksigent Technologies, Llc Electrokinetic pump having capacitive electrodes
US7214348B2 (en) * 2002-07-26 2007-05-08 Applera Corporation Microfluidic size-exclusion devices, systems, and methods
EP1535061A2 (en) 2002-08-21 2005-06-01 Shell Internationale Researchmaatschappij B.V. Method for measuring fluid chemistry in drilling and production operations
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
US7534601B2 (en) * 2002-08-27 2009-05-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
AU2003268201A1 (en) * 2002-08-27 2004-03-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
US7790443B2 (en) * 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
US7595303B1 (en) 2002-09-05 2009-09-29 University Of South Florida Genetic adjuvants for immunotherapy
US7094345B2 (en) * 2002-09-09 2006-08-22 Cytonome, Inc. Implementation of microfluidic components, including molecular fractionation devices, in a microfluidic system
ITTO20020808A1 (it) * 2002-09-17 2004-03-18 St Microelectronics Srl Dispositivo integrato di analisi del dna.
US7279134B2 (en) * 2002-09-17 2007-10-09 Intel Corporation Microfluidic devices with porous membranes for molecular sieving, metering, and separations
ITTO20020809A1 (it) * 2002-09-17 2004-03-18 St Microelectronics Srl Micropompa, in particolare per un dispositivo integrato di analisi del dna.
AU2003267255A1 (en) * 2002-09-20 2004-04-08 Novus Molecular, Inc. Methods and devices for active bioassay
US20040115830A1 (en) * 2002-09-25 2004-06-17 Igor Touzov Components for nano-scale Reactor
US20060183106A1 (en) * 2002-10-18 2006-08-17 Adam Siddiqui-Jain Processes for identifying quadruplex-targeted antiviral molecules
US7179357B2 (en) * 2002-10-25 2007-02-20 Beckman Coulter, Inc. Serial sample injection in capillary electrophoresis
GB2395006A (en) * 2002-10-29 2004-05-12 Micro Chemical Systems Ltd Apparatus and method for performing an assay
JP4346893B2 (ja) * 2002-11-01 2009-10-21 株式会社日立製作所 化学反応装置
WO2004041202A2 (en) * 2002-11-04 2004-05-21 Transform Pharmaceuticals, Inc. Analysis of pharmaceutical solubility and stability
US7108775B2 (en) * 2002-11-08 2006-09-19 Applera Corporation Apparatus and method for confining eluted samples in electrophoresis systems
KR20020097093A (ko) * 2002-11-09 2002-12-31 신세현 자연대류형 미소유체 혼합기
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
US6759713B2 (en) * 2002-11-22 2004-07-06 Xerox Corporation Electronic device structures including interconnecting microfluidic channels
CA2508475C (en) * 2002-12-04 2011-08-30 Spinx, Inc. Devices and methods for programmable microscale manipulation of fluids
WO2004052540A2 (en) * 2002-12-05 2004-06-24 Protasis Corporation Configurable microfluidic substrate assembly
US7507376B2 (en) * 2002-12-19 2009-03-24 3M Innovative Properties Company Integrated sample processing devices
US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
US20060105388A1 (en) * 2002-12-23 2006-05-18 Febit Biothch Gmbh Incorporation of hapten groups during the production of carriers for the determination of analytes
EP1594694A4 (en) * 2002-12-30 2010-01-20 Univ California METHOD AND DEVICE FOR DETECTING AND ANALYZING DISEASES OF DISEASE
CA2552358A1 (en) * 2002-12-31 2004-07-15 Telaura Inc. System and method for real-time detection and remote monitoring of pathogens
US20040129676A1 (en) * 2003-01-07 2004-07-08 Tan Roy H. Apparatus for transfer of an array of liquids and methods for manufacturing same
US7122153B2 (en) * 2003-01-08 2006-10-17 Ho Winston Z Self-contained microfluidic biochip and apparatus
US20050221281A1 (en) * 2003-01-08 2005-10-06 Ho Winston Z Self-contained microfluidic biochip and apparatus
US7332129B2 (en) * 2003-01-09 2008-02-19 3M Innovative Properties Company Sample processing device having process chambers with bypass slots
ATE489629T1 (de) * 2003-01-16 2010-12-15 Caprotec Bioanalytics Gmbh Verfahren zur identifizierung von auf arzneimittel nicht-zielgerichteten proteinen
TW571101B (en) 2003-01-21 2004-01-11 Ind Tech Res Inst Fluid analysis apparatus
WO2004065995A2 (en) * 2003-01-23 2004-08-05 Alpha Innotech Corporation Identifying indicia and focusing target
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
ATE452986T1 (de) 2003-03-17 2010-01-15 Charles River Lab Inc Methoden und zusammensetzungen für den nachweis mikrobieller verunreinigungen
SE0300823D0 (sv) 2003-03-23 2003-03-23 Gyros Ab Preloaded Microscale Devices
SE0300822D0 (sv) 2003-03-23 2003-03-23 Gyros Ab A collection of Micro Scale Devices
US20040223874A1 (en) * 2003-03-31 2004-11-11 Canon Kabushiki Kaisha Biochemical reaction cartridge
CA2521171C (en) 2003-04-03 2013-05-28 Fluidigm Corp. Microfluidic devices and methods of using same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7476363B2 (en) 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
CA2521862C (en) 2003-04-10 2012-10-16 President And Fellows Of Harvard College Formation and control of fluidic species
US7422725B2 (en) * 2003-05-01 2008-09-09 Enplas Corporation Sample handling unit applicable to microchip, and microfluidic device having microchips
US7217396B2 (en) * 2003-05-05 2007-05-15 The Board Of Trustees Of The University Of Illinois Microfabricated micro fluid channels
US7038472B1 (en) 2003-05-12 2006-05-02 Caliper Life Sciences, Inc. Methods and systems for measuring internal dimensions of microscale structures
US20040228962A1 (en) * 2003-05-16 2004-11-18 Chang Liu Scanning probe microscopy probe and method for scanning probe contact printing
US7578916B2 (en) * 2004-05-25 2009-08-25 Applied Biosystems, Llc Emulsions of ionic liquids
US7572409B2 (en) * 2003-05-23 2009-08-11 Applied Biosystems, Llc Ionic liquid apparatus and method for biological samples
CA2527342A1 (en) * 2003-06-05 2004-12-16 Bioprocessors Corp. System and method for process automation
US20040248306A1 (en) * 2003-06-09 2004-12-09 Hernandez Juan J. Microfluidic water analytical device
US7736889B2 (en) * 2003-06-10 2010-06-15 The United States Of America As Represented By The Secretary Of The Navy Fluidic force discrimination
US7672791B2 (en) * 2003-06-13 2010-03-02 International Business Machines Corporation Method of performing three-dimensional molecular superposition and similarity searches in databases of flexible molecules
JP2005007529A (ja) * 2003-06-19 2005-01-13 Dainippon Screen Mfg Co Ltd マイクロ流体デバイスおよびマイクロ流体デバイスの製造方法
US20060147898A1 (en) * 2003-06-20 2006-07-06 Nitto Denko Corporation Cell microchip
EP1654390B1 (en) 2003-07-25 2009-11-11 Platypus Technologies, LLC Liquid crystal based analyte detection
US20050103713A1 (en) * 2003-07-30 2005-05-19 Ramsey J. M. Devices with small-scale channels and the fabrication thereof by etching
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US20050037508A1 (en) * 2003-08-12 2005-02-17 Juan Hernandez Microfluidic titration apparatus
KR101149880B1 (ko) * 2003-08-13 2012-05-24 루미넥스 코포레이션 유세포 분석기식 측정 시스템의 하나 이상의 파라미터의 제어 방법
EP2662135A3 (en) * 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for mixing droplets in a microchannel
WO2005022147A1 (en) 2003-08-28 2005-03-10 Celula, Inc. Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
US20050095627A1 (en) * 2003-09-03 2005-05-05 The Salk Institute For Biological Studies Multiple antigen detection assays and reagents
WO2005029705A2 (en) 2003-09-18 2005-03-31 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
US20060272738A1 (en) * 2003-09-19 2006-12-07 Gary Lim High density plate filler
US20060233673A1 (en) * 2003-09-19 2006-10-19 Beard Nigel P High density plate filler
US8277760B2 (en) * 2003-09-19 2012-10-02 Applied Biosystems, Llc High density plate filler
US20050220675A1 (en) * 2003-09-19 2005-10-06 Reed Mark T High density plate filler
US20050237528A1 (en) * 2003-09-19 2005-10-27 Oldham Mark F Transparent heater for thermocycling
US7998435B2 (en) * 2003-09-19 2011-08-16 Life Technologies Corporation High density plate filler
US7407630B2 (en) * 2003-09-19 2008-08-05 Applera Corporation High density plate filler
US20060233671A1 (en) * 2003-09-19 2006-10-19 Beard Nigel P High density plate filler
US20050226782A1 (en) * 2003-09-19 2005-10-13 Reed Mark T High density plate filler
US9492820B2 (en) 2003-09-19 2016-11-15 Applied Biosystems, Llc High density plate filler
US7695688B2 (en) * 2003-09-19 2010-04-13 Applied Biosystems, Llc High density plate filler
NZ546072A (en) 2003-09-22 2009-08-28 Bioarray Solutions Ltd Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
JP4586021B2 (ja) 2003-09-23 2010-11-24 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル マイクロ流体装置の新規な材料として使用するための光硬化性ペルフルオロポリエーテル
US20050069949A1 (en) * 2003-09-30 2005-03-31 International Business Machines Corporation Microfabricated Fluidic Structures
WO2005032688A1 (en) * 2003-09-30 2005-04-14 Chromba, Inc. Multicapillary column for chromatography and sample preparation
US20070017870A1 (en) 2003-09-30 2007-01-25 Belov Yuri P Multicapillary device for sample preparation
US20050069462A1 (en) * 2003-09-30 2005-03-31 International Business Machines Corporation Microfluidics Packaging
US7718133B2 (en) * 2003-10-09 2010-05-18 3M Innovative Properties Company Multilayer processing devices and methods
JP2007521017A (ja) 2003-10-28 2007-08-02 バイオアレイ ソリューションズ リミテッド 固定化捕捉プローブを用いる遺伝子発現分析法の最適化
JP2007509629A (ja) 2003-10-29 2007-04-19 バイオアレイ ソリューションズ リミテッド 二本鎖ｄｎａの切断による複合核酸分析
CA2545482A1 (en) * 2003-11-10 2005-05-26 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
EP1535665A1 (en) * 2003-11-28 2005-06-01 STMicroelectronics S.r.l. Integrated chemical microreactor with separated channels for confining liquids inside the channels and manufacturing process thereof
EP1541991A1 (en) * 2003-12-12 2005-06-15 STMicroelectronics S.r.l. Integrated semiconductor chemical microreactor for real-time monitoring of biological reactions
US20050161327A1 (en) * 2003-12-23 2005-07-28 Michele Palmieri Microfluidic device and method for transporting electrically charged substances through a microchannel of a microfluidic device
EP1547688A1 (en) * 2003-12-23 2005-06-29 STMicroelectronics S.r.l. Microfluidic device and method of locally concentrating electrically charged substances in a microfluidic device
AU2004312893B2 (en) 2003-12-31 2010-06-17 President And Fellows Of Harvard College Assay device and method
JP3991034B2 (ja) * 2004-01-23 2007-10-17 キヤノン株式会社 検出方法
US8030057B2 (en) * 2004-01-26 2011-10-04 President And Fellows Of Harvard College Fluid delivery system and method
ES2439225T3 (es) 2004-01-26 2014-01-22 President And Fellows Of Harvard College Sistema y método para el suministro de fluidos
SE0400181D0 (sv) * 2004-01-29 2004-01-29 Gyros Ab Segmented porous and preloaded microscale devices
US8058056B2 (en) * 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
WO2005089945A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
US7432106B2 (en) * 2004-03-24 2008-10-07 Applied Biosystems Inc. Liquid processing device including gas trap, and system and method
US20050214737A1 (en) * 2004-03-26 2005-09-29 Dejneka Matthew J Transparent filtered capillaries
US7559356B2 (en) * 2004-04-19 2009-07-14 Eksident Technologies, Inc. Electrokinetic pump driven heat transfer system
WO2005105136A1 (en) * 2004-04-27 2005-11-10 University Of South Florida Nanogene therapy for cell proliferation disorders
ES2553097T3 (es) 2004-05-03 2015-12-04 Handylab, Inc. Procesamiento de muestras que contienen polinucleótidos
JP4683538B2 (ja) 2004-05-06 2011-05-18 セイコーインスツル株式会社 分析用マイクロチップを含む分析システムと分析方法
JP4784508B2 (ja) * 2004-05-07 2011-10-05 コニカミノルタエムジー株式会社 検査用マイクロリアクタおよび検査装置ならびに検査方法
EP1756586B1 (en) * 2004-05-21 2014-07-09 Caliper Life Sciences, Inc. Automated system for handling microfluidic devices
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
EP1779111B1 (en) * 2004-07-16 2010-07-07 Gyros Patent Ab Grading of immune responses
DE602004030678D1 (de) * 2004-07-19 2011-02-03 St Microelectronics Srl Vorrichtung zur Detektion von biologischen Molekülen mit erhöhter Detektionsgeschwindigkeit und Verfahren zur schnellen Detektion von Biomolekülen
EP1622200A1 (en) * 2004-07-26 2006-02-01 CSEM Centre Suisse d'Electronique et de Microtechnique SA Solid-state photodetector pixel and photodetecting method
AU2005330267A1 (en) 2004-07-28 2006-10-12 Canon U.S. Life Sciences, Inc. Methods for monitoring genomic DNA of organisms
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
US20060105453A1 (en) 2004-09-09 2006-05-18 Brenan Colin J Coating process for microfluidic sample arrays
US12070731B2 (en) 2004-08-04 2024-08-27 Life Technologies Corporation Methods and systems for aligning dispensing arrays with microfluidic sample arrays
US7932090B2 (en) * 2004-08-05 2011-04-26 3M Innovative Properties Company Sample processing device positioning apparatus and methods
US7618524B1 (en) 2004-08-10 2009-11-17 Sandia Corporation Polymeric salt bridges for conducting electric current in microfluidic devices
US20060070880A1 (en) * 2004-08-31 2006-04-06 Applera Corporation Methods and apparatus for manipulating separation media
CN102759466A (zh) 2004-09-15 2012-10-31 英特基因有限公司 微流体装置
US7745207B2 (en) 2006-02-03 2010-06-29 IntegenX, Inc. Microfluidic devices
WO2006039293A2 (en) * 2004-09-29 2006-04-13 University Of Virginia Patent Foundation Localized control of thermal properties on microdevices and applications thereof
US7674545B2 (en) * 2004-10-19 2010-03-09 Korea Institute Of Science & Technology Electrokinetic micro power cell using microfluidic-chip with multi-channel type
EP1842069A2 (en) 2004-12-02 2007-10-10 Charles River Laboratories, Inc. Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants
ATE419526T1 (de) 2005-01-13 2009-01-15 Charles River Lab Inc Verfahren zur klassifizierung von mikroben in einer biologischen probe
US8685638B2 (en) * 2005-01-20 2014-04-01 The Regents Of The University Of California Cellular microarrays for screening differentiation factors
US20090101559A1 (en) * 2005-01-21 2009-04-23 Anand Bala Subramaniam Microconcentrator/Microfilter
KR100738071B1 (ko) * 2005-01-21 2007-07-12 삼성전자주식회사 농도구배발생부가 구비된 유전영동 장치, 그를 이용한물질의 분리방법 및 물질 분리의 최적 조건을 탐색하는 방법
CN101506647B (zh) * 2005-01-25 2013-06-12 麻省理工学院 电动浓缩设备及其使用方法
AU2006214085A1 (en) * 2005-02-18 2006-08-24 Canon U.S. Life Sciences, Inc. Devices and methods for identifying genomic DNA of organisms
WO2006096571A2 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
JP2008538077A (ja) * 2005-03-16 2008-10-09 ユニバーシティ オブ シカゴ マイクロフルイディックシステム
JP4548174B2 (ja) * 2005-03-24 2010-09-22 コニカミノルタエムジー株式会社 検査用マイクロチップおよびそれを用いた検査装置
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
AU2006236410B2 (en) 2005-04-19 2012-11-01 President And Fellows Of Harvard College Fluidic structures including meandering and wide channels
US7300631B2 (en) 2005-05-02 2007-11-27 Bioscale, Inc. Method and apparatus for detection of analyte using a flexural plate wave device and magnetic particles
US20070259348A1 (en) * 2005-05-03 2007-11-08 Handylab, Inc. Lyophilized pellets
US20060286549A1 (en) * 2005-05-06 2006-12-21 The Regents Of The University Of California Microfluidic system for identifying or sizing individual particles passing through a channel
NZ620811A (en) 2005-05-09 2015-09-25 Theranos Inc Point-of-care fluidic systems and uses thereof
US8097222B2 (en) * 2005-05-12 2012-01-17 Stmicroelectronics, S.R.L. Microfluidic device with integrated micropump, in particular biochemical microreactor, and manufacturing method thereof
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
US7263874B2 (en) * 2005-06-08 2007-09-04 Bioscale, Inc. Methods and apparatus for determining properties of a fluid
FR2890578B3 (fr) * 2005-09-09 2007-11-30 Rhodia Chimie Sa Dispositif d'ecoulement microfluidique permettant de determiner des parametres d'une transformation physique et/ ou chimique, et son utilisation
JP4892219B2 (ja) * 2005-06-17 2012-03-07 凸版印刷株式会社 反応チップおよび反応装置および反応チップの製造方法
US7763210B2 (en) * 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
USD564667S1 (en) 2005-07-05 2008-03-18 3M Innovative Properties Company Rotatable sample processing disk
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7323660B2 (en) * 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US9637715B2 (en) 2005-07-07 2017-05-02 Emd Millipore Corporation Cell culture and invasion assay method and system
ES2865180T3 (es) * 2005-07-07 2021-10-15 Univ California Aparato para formación de cultivo celular
US9388374B2 (en) 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US9354156B2 (en) 2007-02-08 2016-05-31 Emd Millipore Corporation Microfluidic particle analysis method, device and system
US8257964B2 (en) 2006-01-04 2012-09-04 Cell ASIC Microwell cell-culture device and fabrication method
EP1746410B1 (en) * 2005-07-21 2018-08-22 CSEM Centre Suisse d'Electronique et de Microtechnique SA - Recherche et Développement Apparatus and method for fluorescence lifetime imaging
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
EP1922364A4 (en) 2005-08-09 2010-04-21 Univ North Carolina METHOD AND MATERIALS FOR PRODUCING MICROFLUIDIC DEVICES
WO2007022026A2 (en) * 2005-08-11 2007-02-22 Biotrove, Inc. Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof
EP2660482B1 (en) * 2005-08-22 2019-08-07 Life Technologies Corporation Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen
WO2007025129A2 (en) 2005-08-25 2007-03-01 Platypus Technologies, Llc. Compositions and liquid crystals
WO2007028084A2 (en) 2005-09-01 2007-03-08 Canon U.S. Life Sciences, Inc. Method and molecular diagnostic device for detection, analysis and identification of genomic dna
US7281419B2 (en) * 2005-09-21 2007-10-16 The Board Of Trustees Of The University Of Illinois Multifunctional probe array system
US8822206B2 (en) * 2005-10-11 2014-09-02 The Johns Hopkins University Device for high-throughput stimulation, immunostaining, and visualization of single cells
WO2007044888A2 (en) * 2005-10-11 2007-04-19 The Johns Hopkins Univerisity Microfluidic device and method for high-throughput cellular gradient and dose response studies
US8916375B2 (en) * 2005-10-12 2014-12-23 University Of Virginia Patent Foundation Integrated microfluidic analysis systems
US20090155894A1 (en) * 2005-10-17 2009-06-18 Soper Steven A Electrokinetic Thermal Cycler and Reactor
US20090291437A1 (en) * 2005-11-02 2009-11-26 O'brien Sean Methods for targeting quadruplex sequences
US8794929B2 (en) 2005-11-23 2014-08-05 Eksigent Technologies Llc Electrokinetic pump designs and drug delivery systems
WO2007089541A2 (en) * 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
WO2007098065A2 (en) * 2006-02-17 2007-08-30 The United States Of America As Represented By The Department Of Veterans Affairs Human sodium channel isoforms
EP1830186A1 (en) * 2006-03-01 2007-09-05 ETH Zürich High-throughput cell-based screening system
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
US8741230B2 (en) * 2006-03-24 2014-06-03 Theranos, Inc. Systems and methods of sample processing and fluid control in a fluidic system
WO2007111274A1 (ja) * 2006-03-24 2007-10-04 Kabushiki Kaisha Toshiba 核酸検出カセット及び核酸検出装置
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US8088616B2 (en) 2006-03-24 2012-01-03 Handylab, Inc. Heater unit for microfluidic diagnostic system
GB2436616A (en) * 2006-03-29 2007-10-03 Inverness Medical Switzerland Assay device and method
US8293524B2 (en) * 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US8007999B2 (en) 2006-05-10 2011-08-30 Theranos, Inc. Real-time detection of influenza virus
CN101074963B (zh) * 2006-05-17 2011-07-06 聿新生物科技股份有限公司 电化学胆固醇检测电极测试条和其制造方法
US7674924B2 (en) 2006-05-22 2010-03-09 Third Wave Technologies, Inc. Compositions, probes, and conjugates and uses thereof
US20080070792A1 (en) 2006-06-14 2008-03-20 Roland Stoughton Use of highly parallel snp genotyping for fetal diagnosis
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
JP4292279B2 (ja) * 2006-07-18 2009-07-08 ファルマ・アクセス株式会社 結晶作製器具
US7842499B2 (en) * 2006-08-07 2010-11-30 Platypus Technologies, Llc Substrates, devices, and methods for cellular assays
US8656949B2 (en) 2006-08-15 2014-02-25 University Of Maryland College Park Microfluidic devices and methods of fabrication
US20100178204A1 (en) * 2006-08-21 2010-07-15 Anp Technologies Automated Self-Contained Liquid Handling and Detection System Device
US7674616B2 (en) * 2006-09-14 2010-03-09 Hemosense, Inc. Device and method for measuring properties of a sample
WO2008042399A2 (en) * 2006-10-03 2008-04-10 The Trustees Of The University Of Pennsylvania Method for treatment of macular degeneration
WO2008052138A2 (en) 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same
US8592220B2 (en) 2006-10-26 2013-11-26 Intermolecular, Inc. High pressure parallel fixed bed reactor and method
US20080113391A1 (en) 2006-11-14 2008-05-15 Ian Gibbons Detection and quantification of analytes in bodily fluids
US20080118402A1 (en) * 2006-11-21 2008-05-22 David Brancazio Method and apparatus for analyte processing
US8202491B2 (en) * 2006-11-21 2012-06-19 Bioscale, Inc. Apparatus for analyte processing
US20080153152A1 (en) * 2006-11-22 2008-06-26 Akira Wakabayashi Microfluidic chip
CN1996009B (zh) * 2007-01-10 2010-05-19 博奥生物有限公司 一种用于多样品分析的微流体器件和使用方法
US7867592B2 (en) 2007-01-30 2011-01-11 Eksigent Technologies, Inc. Methods, compositions and devices, including electroosmotic pumps, comprising coated porous surfaces
EP2109666A4 (en) 2007-02-05 2011-09-14 Integenx Inc MICROFLUIDIC AND NANOFLUIDIC DEVICES, SYSTEMS, AND APPLICATIONS
US7776927B2 (en) * 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
US8858770B2 (en) 2007-04-04 2014-10-14 Netbio, Inc. Plastic microfluidic separation and detection platforms
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
ES2687620T3 (es) 2007-05-04 2018-10-26 Opko Diagnostics, Llc Dispositivo y método para análisis en sistemas microfluídicos
US20080297169A1 (en) * 2007-05-31 2008-12-04 Greenquist Alfred C Particle Fraction Determination of A Sample
US8097422B2 (en) 2007-06-20 2012-01-17 Salk Institute For Biological Studies Kir channel modulators
EP2014761B1 (en) * 2007-06-22 2016-09-28 Sony Deutschland GmbH A device for processing an analyte and a method of processing and/or detecting an analyte using said device
US8921121B2 (en) * 2007-06-29 2014-12-30 The Trustees Of Columbia University In The City Of New York Methods, devices, and systems for chemiluminescence-based microfluidic cell counting
USD621060S1 (en) 2008-07-14 2010-08-03 Handylab, Inc. Microfluidic cartridge
US20090136385A1 (en) 2007-07-13 2009-05-28 Handylab, Inc. Reagent Tube
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US8158430B1 (en) 2007-08-06 2012-04-17 Theranos, Inc. Systems and methods of fluidic sample processing
JP5547071B2 (ja) * 2007-08-09 2014-07-09 セルラ・インコーポレイテッド 関連付け多パラメーター単一細胞測定および残留する生物学的材料の回収のための方法および装置
US9968935B2 (en) * 2007-08-20 2018-05-15 Platypus Technologies, Llc Devices for cell assays
JP5289452B2 (ja) * 2007-09-26 2013-09-11 マサチューセッツ インスティテュート オブ テクノロジー 動電学的な濃縮装置及びその使用方法
MX352987B (es) 2007-10-02 2017-12-15 Theranos Ip Co Llc Dispositivos modulares de punto de cuidado y usos de los mismos.
CA2739011A1 (en) * 2007-10-29 2009-05-07 The Regents Of The University Of California Osteoarthritis gene therapy
WO2009076134A1 (en) * 2007-12-11 2009-06-18 Eksigent Technologies, Llc Electrokinetic pump with fixed stroke volume
EP3173787B1 (en) 2008-01-03 2021-06-02 EMD Millipore Corporation Cell culture array system for automated assays and methods of operation and manufacture thereof
CN101990516B (zh) 2008-01-22 2015-09-09 英特基因有限公司 多用试样准备系统及其在集成分析系统中的使用
JP4556194B2 (ja) * 2008-02-01 2010-10-06 セイコーエプソン株式会社 生体試料反応方法
US8397762B2 (en) * 2008-02-04 2013-03-19 Bioscale, Inc. Fluidic system with improved flow characteristics
EP2274098B1 (en) * 2008-03-28 2013-12-25 Biotix, Inc. Multicapillary sample preparation devices and methods for processing analytes
WO2009137244A1 (en) * 2008-04-15 2009-11-12 Charles River Laboratories, Inc. Cartridge and method for sample analysis
CN102036652B (zh) * 2008-04-25 2016-04-13 西北大学 适于螯合胆甾醇的纳米结构
WO2009131677A1 (en) 2008-04-25 2009-10-29 Claros Diagnostics, Inc. Flow control in microfluidic systems
US8472683B2 (en) * 2008-05-09 2013-06-25 General Electric Company Motion correction in tomographic images
US9879360B2 (en) * 2008-06-20 2018-01-30 International Business Machines Corporation Microfluidic selection of library elements
US9393566B2 (en) * 2008-06-23 2016-07-19 Canon U.S. Life Sciences, Inc. System and method for temperature referencing for melt curve data collection
USD618820S1 (en) 2008-07-11 2010-06-29 Handylab, Inc. Reagent holder
EP2344879A4 (en) 2008-09-15 2013-03-20 Platypus Technologies Llc DETECTION OF STEAM PHASE COMPOUNDS BY CHANGING THE PHYSICAL PROPERTIES OF A LIQUID CRYSTAL
ES2620012T3 (es) 2008-09-20 2017-06-27 The Board Of Trustees Of The Leland Stanford Junior University Diagnóstico no invasivo de la aneuploidia fetal por secuenciación
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
WO2011120006A1 (en) 2010-03-25 2011-09-29 Auantalife, Inc. A Delaware Corporation Detection system for droplet-based assays
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
EP2376226B1 (en) 2008-12-18 2018-09-12 Opko Diagnostics, LLC Improved reagent storage in microfluidic systems and related articles and methods
US8448499B2 (en) 2008-12-23 2013-05-28 C A Casyso Ag Cartridge device for a measuring system for measuring viscoelastic characteristics of a sample liquid, a corresponding measuring system, and a corresponding method
EP2384429A1 (en) 2008-12-31 2011-11-09 Integenx Inc. Instrument with microfluidic chip
AU2010208085B2 (en) * 2009-01-30 2014-02-06 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
EP2391714B2 (en) 2009-01-30 2019-07-24 Whitehead Institute for Biomedical Research Methods for ligation and uses thereof
DK2391451T3 (en) 2009-02-02 2018-10-15 Opko Diagnostics Llc STRUCTURES FOR MANAGING LIGHT INTERACTION WITH MICROFLUIDIC DEVICES
EP2394175B1 (en) * 2009-02-09 2016-02-03 caprotec bioanalytics GmbH Devices, systems and methods for separating magnetic particles
CN102341693B (zh) * 2009-03-07 2013-11-20 惠普开发有限公司 分析器和使用该分析器进行感测的方法
PT3299800T (pt) * 2009-03-16 2020-03-04 Alere Tech Gmbh Dispositivo microfluídico
WO2010118427A1 (en) 2009-04-10 2010-10-14 Canon U.S. Life Sciences, Inc. Fluid interface cartridge for a microfluidic chip
US8790916B2 (en) 2009-05-14 2014-07-29 Genestream, Inc. Microfluidic method and system for isolating particles from biological fluid
DE102009040151B4 (de) * 2009-05-26 2013-09-12 Analytik Jena Ag Anordnung zur Detektion von Chemolumineszenz an Gasen
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
CN102459565A (zh) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
CA2764678C (en) 2009-06-04 2017-12-12 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
EP2438016B1 (en) 2009-06-05 2021-06-02 IntegenX Inc. Universal sample preparation system and use in an integrated analysis system
JP2012529908A (ja) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド 法医学的ｄｎａの定量化のための改善された方法
SG177726A1 (en) 2009-07-20 2012-02-28 Siloam Biosciences Inc Microfluidic assay platforms
BR112012004719A2 (pt) 2009-09-02 2016-04-05 Harvard College emulsões múltiplas criadas por uso de jateamento e outras técnicas
MY165876A (en) 2009-10-19 2018-05-18 Theranos Inc Integrated health data capture and analysis system
KR20120102674A (ko) 2009-11-03 2012-09-18 유니버시티 오브 버지니아 페이턴트 파운데이션 중합체 분석물을 검출하는 다용도의 가시적인 방법
CN107028886A (zh) 2009-11-04 2017-08-11 不列颠哥伦比亚大学 含有核酸的脂质粒子及相关的方法
US20110143378A1 (en) * 2009-11-12 2011-06-16 CyVek LLC. Microfluidic method and apparatus for high performance biological assays
USD638550S1 (en) 2009-11-13 2011-05-24 3M Innovative Properties Company Sample processing disk cover
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
USD667561S1 (en) 2009-11-13 2012-09-18 3M Innovative Properties Company Sample processing disk cover
USD638951S1 (en) 2009-11-13 2011-05-31 3M Innovative Properties Company Sample processing disk cover
US9855735B2 (en) 2009-11-23 2018-01-02 Cyvek, Inc. Portable microfluidic assay devices and methods of manufacture and use
US10065403B2 (en) 2009-11-23 2018-09-04 Cyvek, Inc. Microfluidic assay assemblies and methods of manufacture
US9700889B2 (en) 2009-11-23 2017-07-11 Cyvek, Inc. Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results
WO2011063408A1 (en) 2009-11-23 2011-05-26 Cyvek, Inc. Method and apparatus for performing assays
US9759718B2 (en) 2009-11-23 2017-09-12 Cyvek, Inc. PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use
US9500645B2 (en) 2009-11-23 2016-11-22 Cyvek, Inc. Micro-tube particles for microfluidic assays and methods of manufacture
US9651568B2 (en) 2009-11-23 2017-05-16 Cyvek, Inc. Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays
ES2859481T3 (es) 2009-11-24 2021-10-04 Opko Diagnostics Llc Mezcla y suministro de fluidos en sistemas microfluídicos
US8584703B2 (en) * 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US20130034599A1 (en) 2010-01-19 2013-02-07 Northwestern University Synthetic nanostructures including nucleic acids and/or other entities
US9353342B2 (en) 2010-01-21 2016-05-31 Emd Millipore Corporation Cell culture and gradient migration assay methods and devices
KR20130016284A (ko) * 2010-03-17 2013-02-14 바스프 에스이 용융 유화
CN101817495B (zh) * 2010-03-25 2012-03-14 湖南大学 微流控芯片及其制备方法和应用
EP2550362B1 (en) 2010-03-25 2017-01-04 Oregon Health&Science University Cmv glycoproteins and recombinant vectors
JP2011220947A (ja) * 2010-04-14 2011-11-04 Hitachi Engineering & Services Co Ltd 微生物検査装置及び微生物検査チップ
CA2795215C (en) 2010-04-16 2018-11-06 Opko Diagnostics, Llc Systems and devices for analysis of samples
USD645971S1 (en) 2010-05-11 2011-09-27 Claros Diagnostics, Inc. Sample cassette
WO2011150226A1 (en) 2010-05-26 2011-12-01 Landers James P Method for detecting nucleic acids based on aggregate formation
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
WO2012011074A2 (en) 2010-07-22 2012-01-26 Hach Company Lab-on-a-chip for alkalinity analysis
EP2606154B1 (en) 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
EP2606242A4 (en) 2010-08-20 2016-07-20 Integenx Inc MICROFLUIDIC DEVICES WITH MECHANICALLY SEALED MEMBRANE VALVES
US10114020B2 (en) 2010-10-11 2018-10-30 Mbio Diagnostics, Inc. System and device for analyzing a fluidic sample
CA2814720C (en) 2010-10-15 2016-12-13 Lockheed Martin Corporation Micro fluidic optic design
CA2825196C (en) 2011-01-21 2021-01-05 Theranos, Inc. Systems and methods for sample use maximization
CA2830533C (en) * 2011-03-22 2020-02-18 Cyvek, Inc. Microfluidic devices and methods of manufacture and use
JP5637300B2 (ja) * 2011-03-28 2014-12-10 学校法人加計学園 試料溶液濃度測定方法及び試料溶液濃度測定装置
US10526572B2 (en) 2011-04-01 2020-01-07 EMD Millipore Corporaticn Cell culture and invasion assay method and system
EP2701719A4 (en) 2011-04-28 2015-04-22 Claire Mitchell METHOD FOR TREATING MACULAR OBGENATION BY MODULATION OF P2Y12 OR P2X7 RECEPTORS
WO2012151289A2 (en) 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system to detect aggregate formation on a substrate
WO2012151268A1 (en) 2011-05-02 2012-11-08 University Of Virginia Patent Foundation Method and system for high throughput optical and label free detection of analytes
CN103813814A (zh) 2011-05-05 2014-05-21 艾克西根特技术有限公司 用于电动输送系统的凝胶联接
CA2835620C (en) 2011-05-12 2021-02-23 Netbio, Inc. Methods and compositions for rapid multiplex amplification of str loci
AU2012255151B2 (en) 2011-05-18 2015-09-03 Diasorin Italia S.P.A. Systems and methods for detecting the presence of a selected volume of material in a sample processing device
MX336625B (es) 2011-05-18 2016-01-26 3M Innovative Properties Co Sistemas y metodos para medicion volumetrica en dispositivo de procesamiento de muestra.
KR101963721B1 (ko) 2011-05-18 2019-03-29 디아소린 에스.피.에이. 샘플 처리 장치 상의 밸빙을 위한 시스템 및 방법
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
RS57397B8 (sr) 2011-06-10 2020-01-31 Univ Oregon Health & Science Cmv glikoproteini i rekombinantni vektori
WO2013066418A1 (en) * 2011-06-14 2013-05-10 Corning Incorporated Hybrid microfluidic assemblies
CN103764265A (zh) 2011-07-06 2014-04-30 哈佛学院院长等 多重乳剂和用于配制多重乳剂的技术
AU2012216792A1 (en) 2011-09-12 2013-03-28 International Aids Vaccine Initiative Immunoselection of recombinant vesicular stomatitis virus expressing HIV-1 proteins by broadly neutralizing antibodies
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
EP2770980A4 (en) 2011-10-25 2015-11-04 Univ British Columbia LOW SIZE LIPID NANOPARTICLES AND METHODS THEREOF
EP2586461A1 (en) 2011-10-27 2013-05-01 Christopher L. Parks Viral particles derived from an enveloped virus
BR112014010718B1 (pt) 2011-11-16 2020-12-22 Becton, Dickinson And Company métodos, sistemas, dispositivos e kits para detecção de analito em amostra
SG11201402558QA (en) 2011-12-03 2014-06-27 Emd Millipore Corp Micro-incubation systems for microfluidic cell culture and methods
JP6108657B2 (ja) * 2011-12-28 2017-04-05 日本バイリーン株式会社 構造体及びその製造方法
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
SG11201406213WA (en) 2012-03-05 2014-11-27 Arctic Partners Ab Oy Methods and apparatuses for predicting risk of prostate cancer and prostate gland volume
KR102114734B1 (ko) 2012-03-08 2020-05-25 싸이벡, 아이엔씨 미세유체 분석 장치용 마이크로튜브 입자 및 제조방법
EP2838658A1 (en) * 2012-04-16 2015-02-25 QuNano AB A nanocapillary device for biomolecule detection, a fluidic network structure and a method of manufacturing thereof
US9180449B2 (en) 2012-06-12 2015-11-10 Hach Company Mobile water analysis
ES2631608T3 (es) 2012-06-27 2017-09-01 International Aids Vaccine Initiative Variante de la glicoproteína Env del VIH-1
DE102012109026A1 (de) * 2012-09-25 2014-03-27 Eads Deutschland Gmbh Detektionsvorrichtung und Detektionsverfahren zur automatischen Bestimmung von Biomasse
WO2014062719A2 (en) 2012-10-15 2014-04-24 Nanocellect Biomedical, Inc. Systems, apparatus, and methods for sorting particles
US9739714B2 (en) 2012-10-29 2017-08-22 Mbio Diagnostics, Inc. Particle identification system, cartridge and associated methods
USD768872S1 (en) 2012-12-12 2016-10-11 Hach Company Cuvette for a water analysis instrument
JP6296457B2 (ja) 2013-01-11 2018-03-20 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 低コストの臨床現場即時アッセイデバイス
EP2962092A4 (en) * 2013-03-01 2016-08-24 Wave 80 Biosciences Inc METHOD AND SYSTEMS FOR IMPROVED MICROFLUIDIC PROCESSING
US9995412B2 (en) 2013-03-01 2018-06-12 Wave 80 Biosciences, Inc. Long-throw microfluidic actuator
CA2900708C (en) 2013-03-13 2021-06-15 Opko Diagnostics, Llc Mixing of fluids in fluidic systems
EP2971013B1 (en) 2013-03-15 2020-08-19 The University Of British Columbia Lipid nanoparticles for transfection and related methods
JP2014240065A (ja) * 2013-05-15 2014-12-25 公立大学法人大阪府立大学 流路構造体および流路構造体の製造方法
EP2848937A1 (en) 2013-09-05 2015-03-18 International Aids Vaccine Initiative Methods of identifying novel HIV-1 immunogens
EP2873423B1 (en) 2013-10-07 2017-05-31 International Aids Vaccine Initiative Soluble hiv-1 envelope glycoprotein trimers
WO2015052034A1 (en) * 2013-10-07 2015-04-16 Devrim Pesen Okvur Microfluidic device for investigation of distance dependent interactions in cell biology
CN106029863A (zh) 2013-11-06 2016-10-12 贝克顿·迪金森公司 微流体性装置和制造和使用其的方法
CN105899936B (zh) 2013-11-13 2019-12-24 贝克顿·迪金森公司 光学成像系统及使用其的方法
EP3071333A4 (en) 2013-11-18 2017-11-15 IntegenX Inc. Cartridges and instruments for sample analysis
US10024845B2 (en) 2013-12-23 2018-07-17 Basf Se Methods for determining modulators of insect transient receptor potential V (TRPV) channel
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
CA2949863C (en) * 2014-06-13 2017-10-31 Michael Ryan Mcneely Capillary pressure re-set mechanism and applications
US11305236B2 (en) 2014-06-13 2022-04-19 Gattaco Inc. Surface tension driven filtration
US10539579B2 (en) 2014-09-29 2020-01-21 C A Casyso Gmbh Blood testing system and method
EP3692914B1 (en) 2014-10-14 2023-12-27 Becton, Dickinson and Company Blood sample management using open cell foam
WO2016060795A1 (en) 2014-10-14 2016-04-21 Becton, Dickinson And Company Blood sample management using open cell foam
CN107106983B (zh) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
US10210410B2 (en) 2014-10-22 2019-02-19 Integenx Inc. Systems and methods for biometric data collections
KR102587637B1 (ko) 2014-12-12 2023-10-10 옵코 다이어그노스틱스, 엘엘씨 성형에 의해 제조되는 유체 시스템을 비롯한, 항온 처리 채널을 포함하는 유체 시스템
US10605767B2 (en) 2014-12-18 2020-03-31 Life Technologies Corporation High data rate integrated circuit with transmitter configuration
JP6426832B2 (ja) 2015-03-10 2018-11-21 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 生物体液の極微標本管理装置
US10174292B2 (en) 2015-03-20 2019-01-08 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
EP3072901A1 (en) 2015-03-23 2016-09-28 International Aids Vaccine Initiative Soluble hiv-1 envelope glycoprotein trimers
US10167502B2 (en) 2015-04-03 2019-01-01 Fluxion Biosciences, Inc. Molecular characterization of single cells and cell populations for non-invasive diagnostics
JP6466775B2 (ja) * 2015-04-30 2019-02-06 シスメックス株式会社 検体分析カートリッジを用いた検体分析方法、検体分析カートリッジ、および、検体分析装置
CN106248929A (zh) * 2015-06-11 2016-12-21 宁波大学 一种以pdms作基片的艾滋病诊断用多通道芯片装置
NL2015130B1 (en) * 2015-07-09 2017-02-01 Mimetas B V Barrier function measurements.
USD804682S1 (en) 2015-08-10 2017-12-05 Opko Diagnostics, Llc Multi-layered sample cassette
CA3109854C (en) 2015-09-01 2023-07-25 Becton, Dickinson And Company Depth filtration device for separating specimen phases
US10227556B2 (en) 2015-09-04 2019-03-12 Wayne State University Cell culture devices for biomimetic and pathomimetic cell cultures
CN108026560A (zh) 2015-09-25 2018-05-11 豪夫迈·罗氏有限公司 在低共熔溶剂中使用分选酶的转酰胺反应
JP6998862B2 (ja) 2015-09-25 2022-02-04 エフ．ホフマン－ラ ロシュ アーゲー 可溶性ソルターゼａ
JP6895953B2 (ja) 2015-09-25 2021-06-30 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ソルターゼａを利用してチオエステルを作製するための方法
US12048753B2 (en) 2015-10-01 2024-07-30 Whitehead Institute For Biomedical Research Labeling of antibodies
US10228367B2 (en) 2015-12-01 2019-03-12 ProteinSimple Segmented multi-use automated assay cartridge
WO2017100457A1 (en) 2015-12-11 2017-06-15 Opko Diagnostics, Llc Fluidic systems involving incubation samples and/or reagents
EP3436578B1 (en) 2016-03-30 2022-01-19 F. Hoffmann-La Roche AG Improved sortase
US11426580B2 (en) 2016-08-12 2022-08-30 Health Discovery Labs Llc Systems and methods for low intensity high efficiency electrical stimulation
CN106362811B (zh) * 2016-08-29 2018-07-13 北京工业大学 一种测量壁面可变形通道中压力变化的微流控芯片
US20210284998A1 (en) 2016-10-03 2021-09-16 Precision Nanosystems Inc. Compositions for Transfecting Resistant Cell Types
CN110035810B (zh) * 2016-11-29 2021-09-28 普诺森公司 用于同时检测大范围蛋白质浓度的方法和装置
WO2018175411A1 (en) 2017-03-20 2018-09-27 Nanocellect Biomedical, Inc. Systems, apparatuses, and methods for cell sorting and flow cytometry
EP4215617B1 (en) 2017-04-07 2024-08-14 Acenxion Biosystems, Inc. System and method for rapid detection of viable microorganisms in liquid media
WO2019023559A1 (en) 2017-07-27 2019-01-31 University Of Southern California OTOPETRIN PROTON CHANNELS AS TARGETS FOR PHARMACEUTICAL DISCOVERY
CN108181458B (zh) * 2018-02-26 2019-05-14 北京华科泰生物技术股份有限公司 一种基于荧光免疫联合检测的微流控芯片及其制备方法和用途
US12133884B2 (en) 2018-05-11 2024-11-05 Beam Therapeutics Inc. Methods of substituting pathogenic amino acids using programmable base editor systems
EP3566772A1 (en) * 2018-05-11 2019-11-13 Agilent Technologies, Inc. (A Delaware Corporation) Immobilizing fluidic sample for inhibiting spatial broadening
CN112368562A (zh) * 2018-07-11 2021-02-12 香港大学 用于液滴的连续和定量收集的自动微流控系统
GB201820870D0 (en) * 2018-12-20 2019-02-06 Cambridge Entpr Ltd Detection of components
CN109647557B (zh) * 2019-02-27 2021-04-02 哈尔滨工业大学 基于诱导电荷电渗微旋涡的直接颗粒分离芯片及其应用和分离方法
EP4150336A4 (en) * 2020-05-14 2024-06-26 Bifrost Biosystems MICROFLUIDIC DEVICE HAVING SPECIFICALLY DESIGNED DETECTION CHAMBERS
WO2022109390A1 (en) * 2020-11-22 2022-05-27 Gattaco Inc. Sample transfer devices, and components and methods thereof
US20230131266A1 (en) * 2021-10-21 2023-04-27 Hewlett-Packard Development Company, L.P. Alignment and fusion of cells with an electrical field
DE102024106741B4 (de) * 2024-03-08 2025-10-09 Schott Ag Mikroarrays mit Nitrozellulosebeschichtung und Herstellungsverfahren
WO2025197314A1 (ja) * 2024-03-19 2025-09-25 パナソニックＩｐマネジメント株式会社 製造装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996004547A1 (en) * 1994-08-01 1996-02-15 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis
WO1996015576A1 (en) * 1994-11-10 1996-05-23 David Sarnoff Research Center, Inc. Liquid distribution system
WO1996015450A1 (en) * 1994-11-10 1996-05-23 David Sarnoff Research Center, Inc. A partitioned microelectronic device array

Family Cites Families (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3018224A (en) 1957-08-23 1962-01-23 Technicon Instr Analysis and production-monitoring methods and apparatus
NL235965A (enExample) 1958-03-07
US3065148A (en) 1959-07-14 1962-11-20 Technicon Instr Method and apparatus for use in conducting studies on cells
US3701716A (en) 1969-04-15 1972-10-31 Beckman Instruments Inc Liquid analysis apparatus
US3615241A (en) 1970-02-27 1971-10-26 Nasa Firefly pump-metering system
US3699004A (en) 1970-08-31 1972-10-17 Technicon Instr Method and apparatus for sample analysis on a continuous flow basis
US3918908A (en) 1974-05-17 1975-11-11 Geomet Method for prothrombin testing
US4443408A (en) 1981-07-09 1984-04-17 International Technidyne, Inc. Apparatus for analyzing the influence of additive reagents upon the coagulation of blood
US4390403A (en) 1981-07-24 1983-06-28 Batchelder J Samuel Method and apparatus for dielectrophoretic manipulation of chemical species
US4629686A (en) 1982-02-19 1986-12-16 Endotronics, Inc. Apparatus for delivering a controlled dosage of a chemical substance
US4618586A (en) 1983-04-08 1986-10-21 Endotronics, Inc. Apparatus for administering a controlled dosage of a chemical substance having an improved culture chamber
US4554839A (en) 1983-10-14 1985-11-26 Cetus Corporation Multiple trough vessel for automated liquid handling apparatus
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
DE3577748D1 (de) 1984-06-13 1990-06-21 Unilever Nv Vorrichtungen zur verwendung in chemischen analyseverfahren.
US4952518A (en) 1984-10-01 1990-08-28 Cetus Corporation Automated assay machine and assay tray
US5096807A (en) * 1985-03-06 1992-03-17 Murex Corporation Imaging immunoassay detection system with background compensation and its use
US5140161A (en) 1985-08-05 1992-08-18 Biotrack Capillary flow device
US5144139A (en) 1985-08-05 1992-09-01 Biotrack, Inc. Capillary flow device
US5164598A (en) 1985-08-05 1992-11-17 Biotrack Capillary flow device
US4963498A (en) 1985-08-05 1990-10-16 Biotrack Capillary flow device
US4756884A (en) * 1985-08-05 1988-07-12 Biotrack, Inc. Capillary flow device
US4675300A (en) 1985-09-18 1987-06-23 The Board Of Trustees Of The Leland Stanford Junior University Laser-excitation fluorescence detection electrokinetic separation
US4737464A (en) * 1985-09-26 1988-04-12 Molecular Devices Corporation Solid-state optical assay imaging apparatus
US5108703A (en) 1986-03-26 1992-04-28 Beckman Instruments, Inc. Automated multi-purpose analytical chemistry processing center and laboratory work station
US4915812A (en) * 1986-06-20 1990-04-10 Molecular Devices Corporation Zero volume cell
US4783413A (en) 1986-07-30 1988-11-08 Contraves Ag Apparatus for supplying a medium to a reaction chamber
CA1296622C (en) * 1986-08-12 1992-03-03 Jeffrey E. Anderson Method and apparatus for automated assessment of the immunoregulatory status of the mononuclear leukocyte immune system
US4740708A (en) 1987-01-06 1988-04-26 International Business Machines Corporation Semiconductor wafer surface inspection apparatus and method
US5079600A (en) * 1987-03-06 1992-01-07 Schnur Joel M High resolution patterning on solid substrates
US4849340A (en) * 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
US4908112A (en) 1988-06-16 1990-03-13 E. I. Du Pont De Nemours & Co. Silicon semiconductor wafer for analyzing micronic biological samples
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
EP0394406B1 (en) * 1988-10-21 1995-04-26 Molecular Devices Corporation Methods and apparatus for detecting the effect of cell affecting agents on living cells
US5278048A (en) * 1988-10-21 1994-01-11 Molecular Devices Corporation Methods for detecting the effect of cell affecting agents on living cells
US5032381A (en) 1988-12-20 1991-07-16 Tropix, Inc. Chemiluminescence-based static and flow cytometry
DK374889D0 (da) 1989-07-28 1989-07-28 Koege Kemisk Vaerk Fremgangsmaade til procesovervaagning
SE464763B (sv) 1989-10-05 1991-06-10 Gunnar Engstroem Metod och anordning foer att studera cellers/cellaggregats reaktionsmoenster vid perfusion med ett testmedium
IE903930A1 (en) 1989-11-06 1991-05-08 Akzo Nv Immunoassays for and monoclonal antibodies to prothrombin¹activation peptides and their degradation products
US5279936A (en) * 1989-12-22 1994-01-18 Syntex (U.S.A.) Inc. Method of separation employing magnetic particles and second medium
US5171132A (en) 1989-12-27 1992-12-15 Seiko Epson Corporation Two-valve thin plate micropump
US5126022A (en) 1990-02-28 1992-06-30 Soane Tecnologies, Inc. Method and device for moving molecules by the application of a plurality of electrical fields
US5770029A (en) 1996-07-30 1998-06-23 Soane Biosciences Integrated electrophoretic microdevices
US5858188A (en) * 1990-02-28 1999-01-12 Aclara Biosciences, Inc. Acrylic microchannels and their use in electrophoretic applications
US5750015A (en) 1990-02-28 1998-05-12 Soane Biosciences Method and device for moving molecules by the application of a plurality of electrical fields
US5104804A (en) 1990-06-04 1992-04-14 Molecular Devices Corporation Cell assay device used in a microphysiometer
WO1992001160A1 (en) 1990-07-10 1992-01-23 Westonbridge International Limited Valve and micropump incorporating said valve
US5147606A (en) 1990-08-06 1992-09-15 Miles Inc. Self-metering fluid analysis device
EP0498863B1 (en) 1990-08-31 1995-07-26 Westonbridge International Limited A valve equipped with a position detector and a micropump incorporating said valve
GB2248891A (en) 1990-10-18 1992-04-22 Westonbridge Int Ltd Membrane micropump
US5192405A (en) 1991-01-11 1993-03-09 Millipore Corporation Process for effecting high efficiency separations by capillary electrophoresis
US5488469A (en) 1991-08-30 1996-01-30 Omron Corporation Cell analyzing apparatus
GB9119382D0 (en) 1991-09-11 1991-10-23 Knight Scient Ltd Apparatus for monitoring liquids
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5384261A (en) * 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
EP0544969B1 (de) * 1991-12-06 1997-03-05 Ciba-Geigy Ag Elektrophoretische Trennvorrichtung und elektrophoretisches Trennverfahren
US5296144A (en) * 1992-01-02 1994-03-22 World Trade Corporation Composite membrane of a hydrophilic asymmetric membrane coated with an organosiloxane block copolymer
US5451525A (en) 1992-02-14 1995-09-19 Coulter Corporation Method and materials for determining particle count in a flow cytometer
SE500702C2 (sv) 1992-04-07 1994-08-15 Staffan Birnbaum Sätt och anordning för optisk analys av prov separerade i tunna kapillärer
US5223219A (en) 1992-04-10 1993-06-29 Biotrack, Inc. Analytical cartridge and system for detecting analytes in liquid samples
US5744366A (en) 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US5486335A (en) * 1992-05-01 1996-01-23 Trustees Of The University Of Pennsylvania Analysis based on flow restriction
US5296375A (en) 1992-05-01 1994-03-22 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
DE69319427T2 (de) 1992-05-01 1998-12-10 Univ Pennsylvania Analyse bei messung des durchflusswiderstandes
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5726026A (en) 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5304487A (en) 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
GB2266751A (en) 1992-05-02 1993-11-10 Westonbridge Int Ltd Piezoelectric micropump excitation voltage control.
DE4220077A1 (de) 1992-06-19 1993-12-23 Bosch Gmbh Robert Mikropumpe
US5598052A (en) * 1992-07-28 1997-01-28 Philips Electronics North America Vacuum microelectronic device and methodology for fabricating same
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
US5547849A (en) 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
US5441894A (en) 1993-04-30 1995-08-15 Abbott Laboratories Device containing a light absorbing element for automated chemiluminescent immunoassays
US5460709A (en) 1993-06-21 1995-10-24 Helena Laboratories Corporation Automatic electrophoresis method and apparatus
US5395587A (en) * 1993-07-06 1995-03-07 Smithkline Beecham Corporation Surface plasmon resonance detector having collector for eluted ligate
US5429734A (en) * 1993-10-12 1995-07-04 Massachusetts Institute Of Technology Monolithic capillary electrophoretic device
JPH09508353A (ja) 1993-11-02 1997-08-26 アフィマックス テクノロジーズ ナムローゼ フェンノートシャップ 分子多様性の合成及びスクリーニング
DE4411268C2 (de) * 1994-03-31 2001-02-01 Danfoss As Analyseverfahren und Analysevorrichtung
DE4411266C2 (de) * 1994-03-31 2001-05-17 Danfoss As Analyseverfahren und Analysevorrichtung
US5540889A (en) 1994-05-11 1996-07-30 Whitehead Institute For Biomedical Research Apparatus and method for a highly parallel pipetter
DE69527585T2 (de) 1994-06-08 2003-04-03 Affymetrix, Inc. Verfahren und Vorrichtung zum Verpacken von Chips
US5637458A (en) * 1994-07-20 1997-06-10 Sios, Inc. Apparatus and method for the detection and assay of organic molecules
US5645702A (en) 1995-06-07 1997-07-08 Hewlett-Packard Company Low voltage miniaturized column analytical apparatus and method
US5571410A (en) 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5632876A (en) 1995-06-06 1997-05-27 David Sarnoff Research Center, Inc. Apparatus and methods for controlling fluid flow in microchannels
US5603351A (en) 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US5744367A (en) 1994-11-10 1998-04-28 Igen International, Inc. Magnetic particle based electrochemiluminescent detection apparatus and method
EP0739240B1 (en) 1994-11-14 2004-06-16 The Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
DK0739423T3 (da) 1994-11-14 2002-05-06 Univ Pennsylvania Indretning til amplifikation af polynukleotider i mesoskala
US5731212A (en) 1994-12-20 1998-03-24 International Technidyne Corporation Test apparatus and method for testing cuvette accommodated samples
US5560811A (en) 1995-03-21 1996-10-01 Seurat Analytical Systems Incorporated Capillary electrophoresis apparatus and method
US5607565A (en) 1995-03-27 1997-03-04 Coulter Corporation Apparatus for measuring analytes in a fluid sample
US5650075A (en) * 1995-05-30 1997-07-22 Motorola, Inc. Method for etching photolithographically produced quartz crystal blanks for singulation
US5605262A (en) 1995-06-01 1997-02-25 Bond; James R. Adjustable wrist band
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
EP0932671A2 (en) 1995-07-31 1999-08-04 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Extension of a protein-protein interaction surface to inactivate the function of a cellular protein
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
WO1997017426A1 (en) 1995-11-08 1997-05-15 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
US5783397A (en) 1995-12-11 1998-07-21 Northeastern University Screening natural samples for new therapeutic compounds using capillary electrophoresis
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
US6399023B1 (en) 1996-04-16 2002-06-04 Caliper Technologies Corp. Analytical system and method
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US5824204A (en) * 1996-06-27 1998-10-20 Ic Sensors, Inc. Micromachined capillary electrophoresis device
NZ333346A (en) 1996-06-28 2000-03-27 Caliper Techn Corp High-throughput screening assay systems in microscale fluidic devices
US6547942B1 (en) 1996-06-28 2003-04-15 Caliper Technologies Corp. Electropipettor and compensation means for electrophoretic bias
US5779868A (en) * 1996-06-28 1998-07-14 Caliper Technologies Corporation Electropipettor and compensation means for electrophoretic bias
US5800690A (en) 1996-07-03 1998-09-01 Caliper Technologies Corporation Variable control of electroosmotic and/or electrophoretic forces within a fluid-containing structure via electrical forces
US5699157A (en) 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
US5804436A (en) 1996-08-02 1998-09-08 Axiom Biotechnologies, Inc. Apparatus and method for real-time measurement of cellular response
US5858187A (en) 1996-09-26 1999-01-12 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing electrodynamic focusing on a microchip
US6447727B1 (en) 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
JP2001521622A (ja) 1997-04-04 2001-11-06 カリパー テクノロジーズ コーポレイション 閉ループ生化学分析器
CN1105914C (zh) 1997-04-25 2003-04-16 卡钳技术有限公司 改进了通道几何结构的微型流体装置
US6090251A (en) 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
US5869004A (en) * 1997-06-09 1999-02-09 Caliper Technologies Corp. Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems
CA2291854A1 (en) 1997-06-09 1998-12-17 Steven A. Sundberg Apparatus and methods for correcting for variable velocity in microfluidic systems
US5882465A (en) 1997-06-18 1999-03-16 Caliper Technologies Corp. Method of manufacturing microfluidic devices
US5959291A (en) 1997-06-27 1999-09-28 Caliper Technologies Corporation Method and apparatus for measuring low power signals
US5876675A (en) 1997-08-05 1999-03-02 Caliper Technologies Corp. Microfluidic devices and systems
US5989402A (en) 1997-08-29 1999-11-23 Caliper Technologies Corp. Controller/detector interfaces for microfluidic systems
JP2001515204A (ja) 1997-09-02 2001-09-18 カリパー テクノロジーズ コーポレイション 電気流体制御および電気熱制御を有するマイクロ流体システム
US6012902A (en) 1997-09-25 2000-01-11 Caliper Technologies Corp. Micropump
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6022733A (en) 1997-12-02 2000-02-08 Tam; Yun K. Simulated biological dissolution and absorption system
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
US5948227A (en) 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6132685A (en) 1998-08-10 2000-10-17 Caliper Technologies Corporation High throughput microfluidic systems and methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996004547A1 (en) * 1994-08-01 1996-02-15 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis and synthesis
WO1996015576A1 (en) * 1994-11-10 1996-05-23 David Sarnoff Research Center, Inc. Liquid distribution system
WO1996015450A1 (en) * 1994-11-10 1996-05-23 David Sarnoff Research Center, Inc. A partitioned microelectronic device array

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J C WEAVER ET AL: "Gel microdroplets: Rapid detection & enumeration of individual microorganisms by their metabolic activity", BIO/TECHNOLOGY, vol. 6, September 1988 (1988-09-01), pages 1084 - 1089, XP002044138 *

Cited By (446)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558944B1 (en) 1996-06-28 2003-05-06 Caliper Technologies Corp. High throughput screening assay systems in microscale fluidic devices
US6306659B1 (en) 1996-06-28 2001-10-23 Caliper Technologies Corp. High throughput screening assay systems in microscale fluidic devices
US6429025B1 (en) 1996-06-28 2002-08-06 Caliper Technologies Corp. High-throughput screening assay systems in microscale fluidic devices
US7091048B2 (en) 1996-06-28 2006-08-15 Parce J Wallace High throughput screening assay systems in microscale fluidic devices
US6007690A (en) * 1996-07-30 1999-12-28 Aclara Biosciences, Inc. Integrated microfluidic devices
US6440722B1 (en) 1997-04-04 2002-08-27 Caliper Technologies Corp. Microfluidic devices and methods for optimizing reactions
US6670133B2 (en) 1997-04-04 2003-12-30 Caliper Technologies Corp. Microfluidic device for sequencing by hybridization
US6406893B1 (en) 1997-04-04 2002-06-18 Caliper Technologies Corp. Microfluidic methods for non-thermal nucleic acid manipulations
US6403338B1 (en) 1997-04-04 2002-06-11 Mountain View Microfluidic systems and methods of genotyping
US6849411B2 (en) 1997-04-04 2005-02-01 Caliper Life Sciences, Inc. Microfluidic sequencing methods
US7238323B2 (en) 1997-04-04 2007-07-03 Caliper Life Sciences, Inc. Microfluidic sequencing systems
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6444461B1 (en) 1997-04-04 2002-09-03 Caliper Technologies Corp. Microfluidic devices and methods for separation
AU760310B2 (en) * 1997-04-14 2003-05-15 Caliper Life Sciences, Inc. Controlled fluid transport in microfabricated polymeric substrates
EP0975474A4 (en) * 1997-04-14 2000-08-02 Caliper Techn Corp CONTROLLED LIQUID TRANSPORT IN POLYMER SUBSTRATES THROUGH MICROCHANNELS
US6153073A (en) * 1997-04-25 2000-11-28 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
US6068752A (en) * 1997-04-25 2000-05-30 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
US7033474B1 (en) 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
EP2259053A2 (en) 1997-04-25 2010-12-08 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US6613512B1 (en) 1997-06-09 2003-09-02 Caliper Technologies Corp. Apparatus and method for correcting for variable velocity in microfluidic systems
US6524790B1 (en) 1997-06-09 2003-02-25 Caliper Technologies Corp. Apparatus and methods for correcting for variable velocity in microfluidic systems
US6703205B2 (en) 1997-06-09 2004-03-09 Caliper Technologies Corp. Apparatus and methods for correcting for variable velocity in microfluidic systems
US6616823B2 (en) 1997-07-15 2003-09-09 Caliper Technologies Corp. Systems for monitoring and controlling fluid flow rates in microfluidic systems
US6001231A (en) * 1997-07-15 1999-12-14 Caliper Technologies Corp. Methods and systems for monitoring and controlling fluid flow rates in microfluidic systems
US6221226B1 (en) 1997-07-15 2001-04-24 Caliper Technologies Corp. Methods and systems for monitoring and controlling fluid flow rates in microfluidic systems
US6827831B1 (en) 1997-08-29 2004-12-07 Callper Life Sciences, Inc. Controller/detector interfaces for microfluidic systems
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US7497994B2 (en) 1998-02-24 2009-03-03 Khushroo Gandhi Microfluidic devices and systems incorporating cover layers
US6756019B1 (en) 1998-02-24 2004-06-29 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
US6488897B2 (en) 1998-02-24 2002-12-03 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
EP2266695A2 (en) 1998-02-24 2010-12-29 Caliper Life Sciences, Inc. Microfluidic devices and systems incorporating cover layers
US6251343B1 (en) 1998-02-24 2001-06-26 Caliper Technologies Corp. Microfluidic devices and systems incorporating cover layers
EP2092989A1 (en) 1998-05-06 2009-08-26 Caliper Life Sciences, Inc. Methods of fabricating polymeric structures incorporating microscale fluidic elements
WO1999061888A3 (en) * 1998-05-22 2001-12-13 California Inst Of Techn Microfabricated cell sorter
US6306590B1 (en) 1998-06-08 2001-10-23 Caliper Technologies Corp. Microfluidic matrix localization apparatus and methods
US7156969B2 (en) 1998-06-08 2007-01-02 Caliper Life Sciences, Inc. Microfluidic matrix localization apparatus and methods
US6274089B1 (en) 1998-06-08 2001-08-14 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
WO1999064836A1 (en) * 1998-06-08 1999-12-16 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
US6551836B1 (en) 1998-06-08 2003-04-22 Caliper Technologies Corp. Microfluidic devices, systems and methods for performing integrated reactions and separations
EP1086369A4 (en) * 1998-06-08 2006-09-27 Caliper Life Sciences Inc MICROFLUIDIC APPARATUS AND METHODS FOR MATRIX LOCALIZATION
US6869799B1 (en) 1998-06-09 2005-03-22 Symyx Technologies, Inc. Methods for screening catalysts in a parallel fixed-bed reactor
US7241424B2 (en) 1998-06-09 2007-07-10 Symyx Technologies, Inc. Apparatus for screening catalysts in a parallel fixed-bed reactor
CN100374283C (zh) * 1998-06-18 2008-03-12 3M创新有限公司 微流体制品及其制造方法
US6514412B1 (en) 1998-06-18 2003-02-04 3M Innovative Properties Company Microstructured separation device
US6907921B2 (en) 1998-06-18 2005-06-21 3M Innovative Properties Company Microchanneled active fluid heat exchanger
US6375871B1 (en) 1998-06-18 2002-04-23 3M Innovative Properties Company Methods of manufacturing microfluidic articles
WO1999065664A1 (en) * 1998-06-18 1999-12-23 3M Innovative Properties Company Microfluidic articles and method of manufacturing same
US6431695B1 (en) 1998-06-18 2002-08-13 3M Innovative Properties Company Microstructure liquid dispenser
US6381846B2 (en) 1998-06-18 2002-05-07 3M Innovative Properties Company Microchanneled active fluid heat exchanger method
EP1088229A4 (en) * 1998-06-25 2002-05-08 Caliper Techn Corp HIGH THROUGHPUT METHODS, SYSTEMS AND APPARATUS FOR CELL SCREENING TEST
US7343248B2 (en) 1998-07-27 2008-03-11 Caliper Life Sciences Distributed database for analytical instruments
US7155344B1 (en) 1998-07-27 2006-12-26 Caliper Life Sciences, Inc. Distributed database for analytical instruments
US7208320B2 (en) 1998-08-05 2007-04-24 Caliper Life Sciences, Inc. Open-field serial to parallel converter
US6540896B1 (en) 1998-08-05 2003-04-01 Caliper Technologies Corp. Open-Field serial to parallel converter
US6495369B1 (en) 1998-08-10 2002-12-17 Caliper Technologies Corp. High throughput microfluidic systems and methods
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6647358B2 (en) 1998-09-14 2003-11-11 Lion Bioscience Ag Pharmacokinetic-based drug design tool and method
US6542858B1 (en) 1998-09-14 2003-04-01 Lion Bioscience Ag Pharmacokinetic-based drug design tool and method
US6996473B2 (en) 1998-09-14 2006-02-07 Lion Bioscience Ag Method for screening and producing compound libraries
US6846668B1 (en) 1998-10-08 2005-01-25 Astrazeneca Ab Microfabricated cell injector
US6808929B1 (en) 1998-10-13 2004-10-26 Astrazeneca Ab Device and method for physiochemical measurements
WO2000022428A1 (en) * 1998-10-13 2000-04-20 Astrazeneca Ab Device
AU2003204460B2 (en) * 1998-10-14 2005-11-10 Caliper Life Sciences, Inc. Microfluidic controller and detector system with self-calibration
US6447661B1 (en) 1998-10-14 2002-09-10 Caliper Technologies Corp. External material accession systems and methods
AU761071B2 (en) * 1998-10-14 2003-05-29 Caliper Life Sciences, Inc. Microfluidic controller and detector system with self-calibration
US6986837B2 (en) 1998-10-14 2006-01-17 Caliper Life Sciences, Inc. Microfluidic controller and detector system with self-calibration
WO2000022424A1 (en) * 1998-10-14 2000-04-20 Caliper Technologies Corp. Microfluidic controller and detector system with self-calibration
AU761071C (en) * 1998-10-14 2004-02-12 Caliper Life Sciences, Inc. Microfluidic controller and detector system with self-calibration
EP1144674A4 (en) * 1999-01-19 2005-07-27 Caliper Life Sciences Inc HIGH PERFORMANCE OPTIMIZED ANALYTICAL SYSTEM
US6416642B1 (en) 1999-01-21 2002-07-09 Caliper Technologies Corp. Method and apparatus for continuous liquid flow in microscale channels using pressure injection, wicking, and electrokinetic injection
US6989128B2 (en) 1999-01-21 2006-01-24 Caliper Life Sciences, Inc. Apparatus for continuous liquid flow in microscale channels using wicking
US7276330B2 (en) 1999-01-28 2007-10-02 Caliper Technologies Corp. Devices, systems and methods for time domain multiplexing of reagents
US9101928B2 (en) 1999-02-23 2015-08-11 Caliper Life Sciences, Inc. Manipulation of microparticles in microfluidic systems
US6632655B1 (en) 1999-02-23 2003-10-14 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
EP2177627A1 (en) * 1999-02-23 2010-04-21 Caliper Life Sciences, Inc. Manipulation of microparticles in microfluidic systems
US7105300B2 (en) 1999-02-23 2006-09-12 Caliper Life Sciences, Inc. Sequencing by incorporation
US7566538B2 (en) 1999-02-23 2009-07-28 Caliper Lifesciences Inc. Sequencing by incorporation
US6613513B1 (en) 1999-02-23 2003-09-02 Caliper Technologies Corp. Sequencing by incorporation
EP1163052A4 (en) * 1999-02-23 2002-08-14 Caliper Techn Corp MANIPULATION OF MICROPARTICLES IN MICROFLUIDIC SYSTEMS
US7344865B2 (en) 1999-02-23 2008-03-18 Caliper Life Sciences, Inc. Sequencing by incorporation
US7150994B2 (en) 1999-03-03 2006-12-19 Symyx Technologies, Inc. Parallel flow process optimization reactor
DE19910392A1 (de) * 1999-03-05 2000-09-07 Inst Physikalische Hochtech Ev Mikrosäulenreaktor
US7118711B1 (en) 1999-03-05 2006-10-10 Clondiag Chip Technologies Gmbh Microcolumn reactor
DE19910392B4 (de) * 1999-03-05 2005-03-17 Clondiag Chip Technologies Gmbh Mikrosäulenreaktor
US6660367B1 (en) 1999-03-08 2003-12-09 Caliper Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6841193B1 (en) 1999-03-08 2005-01-11 Caliper Life Sciences, Inc. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6509059B2 (en) 1999-03-08 2003-01-21 Caliper Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
US6524830B2 (en) 1999-04-06 2003-02-25 Caliper Technologies Corp. Microfluidic devices and systems for performing inefficient fast PCR
WO2000062050A1 (en) * 1999-04-09 2000-10-19 The University Of Manchester Institute Of Science & Technology Electrophoresis method and apparatus with time- or space-modulated sample injection
US7150814B1 (en) 1999-05-11 2006-12-19 Callper Life Sciences, Inc. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
US6458259B1 (en) 1999-05-11 2002-10-01 Caliper Technologies Corp. Prevention of surface adsorption in microchannels by application of electric current during pressure-induced flow
WO2000070353A3 (en) * 1999-05-13 2001-04-26 Caliper Techn Corp High throughput assay systems and methods
WO2000070080A1 (en) 1999-05-17 2000-11-23 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6506609B1 (en) 1999-05-17 2003-01-14 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US6436646B1 (en) 1999-05-21 2002-08-20 Caliper Technologies Corp. Methods of identifying nucleic acid sequences using polycations
US7150966B2 (en) 1999-05-21 2006-12-19 Caliper Life Sciences, Inc. Assay methods and systems
US7122659B2 (en) 1999-05-21 2006-10-17 Caliper Life Sciences, Inc. Assay methods and systems
US6699655B2 (en) 1999-05-21 2004-03-02 Caliper Technologies Corp. Fluorescent polarization assays involving multivalent metal ions and systems
US6689565B2 (en) 1999-05-21 2004-02-10 Caliper Technologies Corp. Assay methods and systems
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6287774B1 (en) 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
US6649358B1 (en) 1999-06-01 2003-11-18 Caliper Technologies Corp. Microscale assays and microfluidic devices for transporter, gradient induced, and binding activities
EP1185871A4 (en) * 1999-06-01 2003-01-15 Caliper Techn Corp MICRO-SCALE ASSAYS AND MICROFLUIDIC DEVICES FOR THE CONTROL OF TRANSPORTER, INDUCED GRADIENT AND BINDING ACTIVITIES
WO2000077507A1 (de) * 1999-06-16 2000-12-21 Merck Patent Gmbh Vorrichtung zur probenaufgabe
JP2001004628A (ja) * 1999-06-18 2001-01-12 Kanagawa Acad Of Sci & Technol 免疫分析装置と免疫分析方法
EP1063204A3 (en) * 1999-06-21 2002-08-21 Micro Chemical Systems Limited Chemical devices, methods of manufacturing and of using chemical devices
US6902706B1 (en) * 1999-06-22 2005-06-07 Biomerieux S.A. Valves enabling a liquid to be directed in a diagnostic chart diagnostic charts and diagnostic device comprising several charts
WO2000078453A1 (fr) * 1999-06-22 2000-12-28 Biomerieux S.A. Vannes permettant de diriger un fluide dans une carte d'analyse, cartes d'analyse et dispositif d'analyse comprenant plusieurs cartes
US7223364B1 (en) 1999-07-07 2007-05-29 3M Innovative Properties Company Detection article having fluid control film
DE19935433A1 (de) * 1999-08-01 2001-03-01 Febit Ferrarius Biotech Gmbh Mikrofluidischer Reaktionsträger
US7361314B1 (en) 1999-08-01 2008-04-22 Febit Biotech Gmbh Microfluid reaction carrier having three flow levels and a transparent protective layer
WO2001012327A1 (en) * 1999-08-12 2001-02-22 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US7238268B2 (en) 1999-08-12 2007-07-03 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
AU768135B2 (en) * 1999-08-12 2003-12-04 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US6858185B1 (en) 1999-08-25 2005-02-22 Caliper Life Sciences, Inc. Dilutions in high throughput systems with a single vacuum source
US6613581B1 (en) 1999-08-26 2003-09-02 Caliper Technologies Corp. Microfluidic analytic detection assays, devices, and integrated systems
US6613211B1 (en) 1999-08-27 2003-09-02 Aclara Biosciences, Inc. Capillary electrokinesis based cellular assays
US6468763B1 (en) 1999-09-29 2002-10-22 Caliper Technologies Corp. Optical detection of transmembrane potential changes
US6979553B2 (en) 1999-10-08 2005-12-27 Caliper Life Sciences, Inc. Use of Nernstein voltage sensitive dyes in measuring transmembrane voltage
US6759191B2 (en) 1999-10-08 2004-07-06 Caliper Life Sciences, Inc. Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
US6537771B1 (en) 1999-10-08 2003-03-25 Caliper Technologies Corp. Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
US6271038B1 (en) 1999-12-15 2001-08-07 Glaxo Wellcome Inc. Methods for high throughout determination and ranking of formulations and solubility
US6379884B2 (en) 2000-01-06 2002-04-30 Caliper Technologies Corp. Methods and systems for monitoring intracellular binding reactions
US6468761B2 (en) 2000-01-07 2002-10-22 Caliper Technologies, Corp. Microfluidic in-line labeling method for continuous-flow protease inhibition analysis
US6632629B2 (en) 2000-01-07 2003-10-14 Caliper Technologies Corp. Microfluidic in-line labeling method of continuous-flow protease inhibition analysis
US7037416B2 (en) 2000-01-14 2006-05-02 Caliper Life Sciences, Inc. Method for monitoring flow rate using fluorescent markers
US6556923B2 (en) 2000-01-26 2003-04-29 Caliper Technologies Corp. Software for high throughput microfluidic systems
EP1252508A4 (en) * 2000-01-26 2007-03-21 Caliper Life Sciences Inc SOFTWARE FOR HIGH-THROUGHPUT MICROFLUIDIC SYSTEMS
US7001716B2 (en) 2000-02-04 2006-02-21 Caliper Life Sciences, Inc. Continuous flow methods for monitoring time dependent reactions
US6589729B2 (en) 2000-02-04 2003-07-08 Caliper Technologies Corp. Methods, devices, and systems for monitoring time dependent reactions
US7122156B2 (en) 2000-03-07 2006-10-17 Symyx Technologies, Inc. Parallel flow reactor having variable composition
US7521186B2 (en) 2000-03-20 2009-04-21 Caliper Lifesciences Inc. PCR compatible nucleic acid sieving matrix
JP2001340753A (ja) * 2000-03-29 2001-12-11 Sumitomo Chem Co Ltd 反応方法および反応装置
US6733645B1 (en) 2000-04-18 2004-05-11 Caliper Technologies Corp. Total analyte quantitation
US7264702B1 (en) 2000-04-18 2007-09-04 Caliper Life Sciences, Inc. Total analyte quantitation
US6569607B2 (en) 2000-05-03 2003-05-27 Caliper Technologies Corp. Multi depth substrate fabrication processes
US6669831B2 (en) 2000-05-11 2003-12-30 Caliper Technologies Corp. Microfluidic devices and methods to regulate hydrodynamic and electrical resistance utilizing bulk viscosity enhancers
US6777184B2 (en) 2000-05-12 2004-08-17 Caliper Life Sciences, Inc. Detection of nucleic acid hybridization by fluorescence polarization
US6635487B1 (en) 2000-05-17 2003-10-21 Caliper Technologies Corp. Fluorescence standard for use in microfluidic instruments
US9926521B2 (en) 2000-06-27 2018-03-27 Fluidigm Corporation Microfluidic particle-analysis systems
US7192559B2 (en) 2000-08-03 2007-03-20 Caliper Life Sciences, Inc. Methods and devices for high throughput fluid delivery
JP2004508837A (ja) * 2000-09-14 2004-03-25 カリパー・テクノロジーズ・コープ． 温度介在反応を行うためのマイクロ流体装置及び方法
US9176137B2 (en) 2000-11-16 2015-11-03 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US8455258B2 (en) 2000-11-16 2013-06-04 California Insitute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7887753B2 (en) 2000-11-16 2011-02-15 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US8673645B2 (en) 2000-11-16 2014-03-18 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7378280B2 (en) 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US10509018B2 (en) 2000-11-16 2019-12-17 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US8273574B2 (en) 2000-11-16 2012-09-25 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US7070682B2 (en) 2001-01-16 2006-07-04 Cheng Lee Microfluidic apparatus for performing gel protein extractions and methods for using the apparatus
US9528142B2 (en) 2001-02-14 2016-12-27 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US9051604B2 (en) 2001-02-14 2015-06-09 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
US7118917B2 (en) 2001-03-07 2006-10-10 Symyx Technologies, Inc. Parallel flow reactor having improved thermal control
US10620194B2 (en) 2001-03-19 2020-04-14 Gyros Patent Ab Characterization of reaction variables
US7429354B2 (en) 2001-03-19 2008-09-30 Gyros Patent Ab Structural units that define fluidic functions
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US9259735B2 (en) 2001-03-28 2016-02-16 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US10571935B2 (en) 2001-03-28 2020-02-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US8420015B2 (en) 2001-03-28 2013-04-16 Handylab, Inc. Systems and methods for thermal actuation of microfluidic devices
US10619191B2 (en) 2001-03-28 2020-04-14 Handylab, Inc. Systems and methods for thermal actuation of microfluidic devices
US10351901B2 (en) 2001-03-28 2019-07-16 Handylab, Inc. Systems and methods for thermal actuation of microfluidic devices
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US8936764B2 (en) 2001-04-06 2015-01-20 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US8486636B2 (en) 2001-04-06 2013-07-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US7833708B2 (en) 2001-04-06 2010-11-16 California Institute Of Technology Nucleic acid amplification using microfluidic devices
US6974526B2 (en) * 2001-05-01 2005-12-13 Calibrant Biosystems, Inc. Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
US7641780B2 (en) 2001-05-01 2010-01-05 Calibrant Biosystems, Inc. Two-dimensional microfluidics for protein separations and gene analysis
US6929730B2 (en) * 2001-05-01 2005-08-16 Cheng Sheng Lee Two dimensional microfluidic gene scanner
US7723123B1 (en) 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US8592141B2 (en) 2001-06-05 2013-11-26 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US8007738B2 (en) 2001-06-05 2011-08-30 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US8216852B2 (en) 2001-07-27 2012-07-10 Caliper Life Sciences, Inc. Channel cross-section geometry to manipulate dispersion rates
US9028773B2 (en) 2001-09-12 2015-05-12 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8043581B2 (en) 2001-09-12 2011-10-25 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US8163492B2 (en) 2001-11-30 2012-04-24 Fluidign Corporation Microfluidic device and methods of using same
US7820427B2 (en) 2001-11-30 2010-10-26 Fluidigm Corporation Microfluidic device and methods of using same
US9643178B2 (en) 2001-11-30 2017-05-09 Fluidigm Corporation Microfluidic device with reaction sites configured for blind filling
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
EP1902779A3 (en) * 2002-02-25 2008-04-02 Hitachi Chemical Co., Ltd. A support unit for a microfluidic system
US8889084B2 (en) 2002-02-25 2014-11-18 Hitachi Chemical Company, Ltd. Micro fluid system support and manufacturing method thereof
US8865090B2 (en) 2002-02-25 2014-10-21 Hitachi Chemical Co., Ltd. Micro fluid system support and manufacturing method thereof
EP1902779A2 (en) 2002-02-25 2008-03-26 Hitachi Chemical Co., Ltd. A support unit for a microfluidic system
EP2581739A1 (en) 2002-03-05 2013-04-17 Caliper Life Sciences, Inc. Mixed mode microfluidic systems
US7160423B2 (en) 2002-03-05 2007-01-09 Caliper Life Sciences, Inc. Mixed mode microfluidic systems
US7252928B1 (en) 2002-03-12 2007-08-07 Caliper Life Sciences, Inc. Methods for prevention of surface adsorption of biological materials to capillary walls in microchannels
US8658418B2 (en) 2002-04-01 2014-02-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7452726B2 (en) 2002-04-01 2008-11-18 Fluidigm Corporation Microfluidic particle-analysis systems
EP2666849A3 (en) * 2002-04-01 2014-05-28 Fluidigm Corporation Microfluidic particle-analysis systems
US11027278B2 (en) 2002-04-17 2021-06-08 Cytonome/St, Llc Methods for controlling fluid flow in a microfluidic system
US9714443B2 (en) 2002-09-25 2017-07-25 California Institute Of Technology Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors
US8372579B2 (en) 2002-09-27 2013-02-12 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US8895298B2 (en) 2002-09-27 2014-11-25 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US11052392B2 (en) 2002-09-27 2021-07-06 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US8986966B2 (en) 2002-09-27 2015-03-24 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US10081014B2 (en) 2002-09-27 2018-09-25 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US8304230B2 (en) 2002-09-27 2012-11-06 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
US10328428B2 (en) 2002-10-02 2019-06-25 California Institute Of Technology Apparatus for preparing cDNA libraries from single cells
US9579650B2 (en) 2002-10-02 2017-02-28 California Institute Of Technology Microfluidic nucleic acid analysis
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US10940473B2 (en) 2002-10-02 2021-03-09 California Institute Of Technology Microfluidic nucleic acid analysis
US8232074B2 (en) 2002-10-16 2012-07-31 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
US8275554B2 (en) 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US10428377B2 (en) 2002-12-20 2019-10-01 Caliper Life Sciences, Inc. Methods of detecting low copy nucleic acids
US7645581B2 (en) 2002-12-20 2010-01-12 Caliper Life Sciences, Inc. Determining nucleic acid fragmentation status by coincident detection of two labeled probes
US11187702B2 (en) 2003-03-14 2021-11-30 Bio-Rad Laboratories, Inc. Enzyme quantification
US10052605B2 (en) 2003-03-31 2018-08-21 Medical Research Council Method of synthesis and testing of combinatorial libraries using microcapsules
US9448172B2 (en) 2003-03-31 2016-09-20 Medical Research Council Selection by compartmentalised screening
US9857303B2 (en) 2003-03-31 2018-01-02 Medical Research Council Selection by compartmentalised screening
US10131934B2 (en) 2003-04-03 2018-11-20 Fluidigm Corporation Thermal reaction device and method for using the same
DE10320869A1 (de) * 2003-05-09 2004-12-16 Evotec Technologies Gmbh Verfahren und Vorrichtungen zur Flüssigkeitsbehandlung suspendierter Partikel
US7582461B2 (en) 2003-07-29 2009-09-01 Life Technologies Corporation Kinase and phosphatase assays
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US7619059B2 (en) 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
US10731201B2 (en) 2003-07-31 2020-08-04 Handylab, Inc. Processing particle-containing samples
US9670528B2 (en) 2003-07-31 2017-06-06 Handylab, Inc. Processing particle-containing samples
US11078523B2 (en) 2003-07-31 2021-08-03 Handylab, Inc. Processing particle-containing samples
US12139745B2 (en) 2003-07-31 2024-11-12 Handylab, Inc. Processing particle-containing samples
US10865437B2 (en) 2003-07-31 2020-12-15 Handylab, Inc. Processing particle-containing samples
US7678336B2 (en) 2003-12-22 2010-03-16 Digital Bio Technology Channel apparatus for focusing a fluid flow
WO2005062059A1 (en) * 2003-12-22 2005-07-07 Digital Bio Technology Channel apparatus for focusing a fluid flow
US9839890B2 (en) 2004-03-31 2017-12-12 National Science Foundation Compartmentalised combinatorial chemistry by microfluidic control
US11821109B2 (en) 2004-03-31 2023-11-21 President And Fellows Of Harvard College Compartmentalised combinatorial chemistry by microfluidic control
US9925504B2 (en) 2004-03-31 2018-03-27 President And Fellows Of Harvard College Compartmentalised combinatorial chemistry by microfluidic control
KR100597870B1 (ko) * 2004-04-01 2006-07-06 한국과학기술원 고속 스크리닝 또는 고속 분석을 위한 미소 유체 칩
US10443088B1 (en) 2004-05-03 2019-10-15 Handylab, Inc. Method for processing polynucleotide-containing samples
US11441171B2 (en) 2004-05-03 2022-09-13 Handylab, Inc. Method for processing polynucleotide-containing samples
US10494663B1 (en) 2004-05-03 2019-12-03 Handylab, Inc. Method for processing polynucleotide-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
US10604788B2 (en) 2004-05-03 2020-03-31 Handylab, Inc. System for processing polynucleotide-containing samples
US10364456B2 (en) 2004-05-03 2019-07-30 Handylab, Inc. Method for processing polynucleotide-containing samples
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US12097475B2 (en) 2004-07-02 2024-09-24 The University Of Chicago Microfluidic system
US8871444B2 (en) 2004-10-08 2014-10-28 Medical Research Council In vitro evolution in microfluidic systems
US11786872B2 (en) 2004-10-08 2023-10-17 United Kingdom Research And Innovation Vitro evolution in microfluidic systems
US9029083B2 (en) 2004-10-08 2015-05-12 Medical Research Council Vitro evolution in microfluidic systems
US9498759B2 (en) 2004-10-12 2016-11-22 President And Fellows Of Harvard College Compartmentalized screening by microfluidic control
US8480971B2 (en) 2004-11-30 2013-07-09 Hitachi Chemical Co., Ltd. Analytical pretreatment device
US8480970B2 (en) 2004-11-30 2013-07-09 Hitachi Chemical Co., Ltd. Analytical pretreatment device
EP2482075A1 (en) 2004-12-01 2012-08-01 Proteologics, Ltd Ubiquitin ligase assays and related reagents
US10994273B2 (en) 2004-12-03 2021-05-04 Cytonome/St, Llc Actuation of parallel microfluidic arrays
US9328344B2 (en) 2006-01-11 2016-05-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9534216B2 (en) 2006-01-11 2017-01-03 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9410151B2 (en) 2006-01-11 2016-08-09 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US12146134B2 (en) 2006-01-11 2024-11-19 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US8420017B2 (en) 2006-02-28 2013-04-16 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
US12162007B2 (en) 2006-03-24 2024-12-10 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US11085069B2 (en) 2006-03-24 2021-08-10 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10843188B2 (en) 2006-03-24 2020-11-24 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US10821436B2 (en) 2006-03-24 2020-11-03 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US10821446B1 (en) 2006-03-24 2020-11-03 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10799862B2 (en) 2006-03-24 2020-10-13 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10913061B2 (en) 2006-03-24 2021-02-09 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US11959126B2 (en) 2006-03-24 2024-04-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US12458972B2 (en) 2006-03-24 2025-11-04 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10695764B2 (en) 2006-03-24 2020-06-30 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10857535B2 (en) 2006-03-24 2020-12-08 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US9080207B2 (en) 2006-03-24 2015-07-14 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9040288B2 (en) 2006-03-24 2015-05-26 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US11142785B2 (en) 2006-03-24 2021-10-12 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11141734B2 (en) 2006-03-24 2021-10-12 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US9802199B2 (en) 2006-03-24 2017-10-31 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US11666903B2 (en) 2006-03-24 2023-06-06 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US11351510B2 (en) 2006-05-11 2022-06-07 Bio-Rad Laboratories, Inc. Microfluidic devices
US9273308B2 (en) 2006-05-11 2016-03-01 Raindance Technologies, Inc. Selection of compartmentalized screening method
US12337287B2 (en) 2006-05-11 2025-06-24 Bio-Rad Laboratories, Inc. Microfluidic devices
US12091710B2 (en) 2006-05-11 2024-09-17 Bio-Rad Laboratories, Inc. Systems and methods for handling microfluidic droplets
EP2636755A1 (en) 2006-05-26 2013-09-11 AltheaDx Incorporated Biochemical analysis of partitioned cells
WO2007140015A2 (en) 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US11378498B2 (en) 2006-06-14 2022-07-05 Verinata Health, Inc. Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US10155984B2 (en) 2006-06-14 2018-12-18 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US11781187B2 (en) 2006-06-14 2023-10-10 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US11215610B2 (en) 2006-10-13 2022-01-04 Labrador Diagnostics Llc Reducing optical interference in a fluidic device
US11442061B2 (en) 2006-10-13 2022-09-13 Labrador Diagnostics Llc Reducing optical interference in a fluidic device
US10067123B2 (en) 2006-10-13 2018-09-04 Theranos Ip Company Llc Reducing optical interference in a fluidic device
US9815057B2 (en) 2006-11-14 2017-11-14 Handylab, Inc. Microfluidic cartridge and method of making same
US12030050B2 (en) 2006-11-14 2024-07-09 Handylab, Inc. Microfluidic cartridge and method of making same
US12128405B2 (en) 2006-11-14 2024-10-29 Handylab, Inc. Microfluidic valve and method of making same
US10710069B2 (en) 2006-11-14 2020-07-14 Handylab, Inc. Microfluidic valve and method of making same
US10603662B2 (en) 2007-02-06 2020-03-31 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008097559A3 (en) * 2007-02-06 2008-10-09 Univ Brandeis Manipulation of fluids and reactions in microfluidic systems
US9440232B2 (en) 2007-02-06 2016-09-13 Raindance Technologies, Inc. Manipulation of fluids and reactions in microfluidic systems
US11819849B2 (en) 2007-02-06 2023-11-21 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US9017623B2 (en) 2007-02-06 2015-04-28 Raindance Technologies, Inc. Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) * 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US11224876B2 (en) 2007-04-19 2022-01-18 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US10675626B2 (en) 2007-04-19 2020-06-09 President And Fellows Of Harvard College Manipulation of fluids, fluid components and reactions in microfluidic systems
US11618024B2 (en) 2007-04-19 2023-04-04 President And Fellows Of Harvard College Manipulation of fluids, fluid components and reactions in microfluidic systems
US10357772B2 (en) 2007-04-19 2019-07-23 President And Fellows Of Harvard College Manipulation of fluids, fluid components and reactions in microfluidic systems
US10960397B2 (en) 2007-04-19 2021-03-30 President And Fellows Of Harvard College Manipulation of fluids, fluid components and reactions in microfluidic systems
US9068699B2 (en) 2007-04-19 2015-06-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
US10179910B2 (en) 2007-07-13 2019-01-15 Handylab, Inc. Rack for sample tubes and reagent holders
US9347586B2 (en) 2007-07-13 2016-05-24 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US11549959B2 (en) 2007-07-13 2023-01-10 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US10234474B2 (en) 2007-07-13 2019-03-19 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US9238223B2 (en) 2007-07-13 2016-01-19 Handylab, Inc. Microfluidic cartridge
US10875022B2 (en) 2007-07-13 2020-12-29 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US12128402B2 (en) 2007-07-13 2024-10-29 Handylab, Inc. Microfluidic cartridge
US10139012B2 (en) 2007-07-13 2018-11-27 Handylab, Inc. Integrated heater and magnetic separator
US9701957B2 (en) 2007-07-13 2017-07-11 Handylab, Inc. Reagent holder, and kits containing same
US10065185B2 (en) 2007-07-13 2018-09-04 Handylab, Inc. Microfluidic cartridge
US10071376B2 (en) 2007-07-13 2018-09-11 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11060082B2 (en) 2007-07-13 2021-07-13 Handy Lab, Inc. Polynucleotide capture materials, and systems using same
US11254927B2 (en) 2007-07-13 2022-02-22 Handylab, Inc. Polynucleotide capture materials, and systems using same
US10632466B1 (en) 2007-07-13 2020-04-28 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11466263B2 (en) 2007-07-13 2022-10-11 Handylab, Inc. Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
US10625262B2 (en) 2007-07-13 2020-04-21 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10844368B2 (en) 2007-07-13 2020-11-24 Handylab, Inc. Diagnostic apparatus to extract nucleic acids including a magnetic assembly and a heater assembly
US10625261B2 (en) 2007-07-13 2020-04-21 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US11266987B2 (en) 2007-07-13 2022-03-08 Handylab, Inc. Microfluidic cartridge
US10100302B2 (en) 2007-07-13 2018-10-16 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US10717085B2 (en) 2007-07-13 2020-07-21 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9217143B2 (en) 2007-07-13 2015-12-22 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US12397295B2 (en) 2007-07-13 2025-08-26 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9259734B2 (en) 2007-07-13 2016-02-16 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US10590410B2 (en) 2007-07-13 2020-03-17 Handylab, Inc. Polynucleotide capture materials, and methods of using same
EP3677336A1 (en) 2007-09-05 2020-07-08 Caliper Life Sciences Inc. Microfluidic method and system for enzyme inhibition activity screening
US9211537B2 (en) 2007-11-07 2015-12-15 The University Of British Columbia Microfluidic device and method of using same
US10828641B2 (en) 2008-04-28 2020-11-10 President And Fellows Of Harvard College Microfluidic device for storage and well-defined arrangement of droplets
US11498072B2 (en) 2008-04-28 2022-11-15 President And Fellows Of Harvard College Microfluidic device for storage and well-defined arrangement of droplets
US9664619B2 (en) 2008-04-28 2017-05-30 President And Fellows Of Harvard College Microfluidic device for storage and well-defined arrangement of droplets
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
US11596908B2 (en) 2008-07-18 2023-03-07 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
US11511242B2 (en) 2008-07-18 2022-11-29 Bio-Rad Laboratories, Inc. Droplet libraries
US11534727B2 (en) 2008-07-18 2022-12-27 Bio-Rad Laboratories, Inc. Droplet libraries
EP2352025A4 (en) * 2008-08-11 2013-08-07 Fujimori Kogyo Co Ltd THROMBOZY TEST METHOD AND THROMBOZYTENTESTVORRICHTUNG
RU2543652C2 (ru) * 2008-08-11 2015-03-10 Фудзимори Когио Ко., Лтд. Способ тестирования тромбоцитов и устройство для тестирования тромбоцитов
US11633739B2 (en) 2008-09-23 2023-04-25 Bio-Rad Laboratories, Inc. Droplet-based assay system
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US11612892B2 (en) 2008-09-23 2023-03-28 Bio-Rad Laboratories, Inc. Method of performing droplet-based assays
US9126160B2 (en) 2008-09-23 2015-09-08 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9248417B2 (en) 2008-09-23 2016-02-02 Bio-Rad Laboratories, Inc. System for droplet-based assays using an array of emulsions
US9216392B2 (en) 2008-09-23 2015-12-22 Bio-Rad Laboratories, Inc. System for forming an array of emulsions
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US11130134B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Method of performing droplet-based assays
US9623384B2 (en) 2008-09-23 2017-04-18 Bio-Rad Laboratories, Inc. System for transporting emulsions from an array to a detector
US9649635B2 (en) 2008-09-23 2017-05-16 Bio-Rad Laboratories, Inc. System for generating droplets with push-back to remove oil
US10279350B2 (en) 2008-09-23 2019-05-07 Bio-Rad Laboratories, Inc. Method of generating droplets
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US10258988B2 (en) 2008-09-23 2019-04-16 Bio-Rad Laboratories, Inc. Device for generating droplets
US10258989B2 (en) 2008-09-23 2019-04-16 Bio-Rad Laboratories, Inc. Method of making a device for generating droplets
US12168231B2 (en) 2008-09-23 2024-12-17 Bio-Rad Laboratories, Inc. Method of analysis
US9243288B2 (en) 2008-09-23 2016-01-26 Bio-Rad Laboratories, Inc. Cartridge with lysis chamber and droplet generator
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US12352673B2 (en) 2009-03-23 2025-07-08 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
US11268887B2 (en) 2009-03-23 2022-03-08 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
US10370705B2 (en) 2009-03-24 2019-08-06 University Of Chicago Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US10543485B2 (en) 2009-03-24 2020-01-28 University Of Chicago Slip chip device and methods
US9415392B2 (en) 2009-03-24 2016-08-16 The University Of Chicago Slip chip device and methods
US9493826B2 (en) 2009-03-24 2016-11-15 California Institute Of Technology Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US10166522B2 (en) 2009-09-02 2019-01-01 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US10677693B2 (en) 2009-09-02 2020-06-09 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US8796031B2 (en) 2010-02-10 2014-08-05 Fujimori Kogyo Co., Ltd. Microchip for platelet examination and platelet examination device using same
US11254968B2 (en) 2010-02-12 2022-02-22 Bio-Rad Laboratories, Inc. Digital analyte analysis
US12454718B2 (en) 2010-02-12 2025-10-28 Bio-Rad Laboratories, Inc. Digital analyte analysis
US10808279B2 (en) 2010-02-12 2020-10-20 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US11390917B2 (en) 2010-02-12 2022-07-19 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US11866771B2 (en) 2010-03-02 2024-01-09 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US11060136B2 (en) 2010-03-02 2021-07-13 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US10378048B2 (en) 2010-03-02 2019-08-13 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US10272432B2 (en) 2010-03-25 2019-04-30 Bio-Rad Laboratories, Inc. Device for generating droplets
US9393560B2 (en) 2010-03-25 2016-07-19 Bio-Rad Laboratories, Inc. Droplet transport system for detection
US10744506B2 (en) 2010-03-25 2020-08-18 Bio-Rad Laboratories, Inc. Device for generating droplets
US9500664B2 (en) 2010-03-25 2016-11-22 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
US10099219B2 (en) 2010-03-25 2018-10-16 Bio-Rad Laboratories, Inc. Device for generating droplets
US12103005B2 (en) 2010-03-25 2024-10-01 Bio-Rad Laboratories, Inc. Method of emulsion formation and modification
US11635427B2 (en) 2010-09-30 2023-04-25 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9089844B2 (en) 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US11077415B2 (en) 2011-02-11 2021-08-03 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
US11768198B2 (en) 2011-02-18 2023-09-26 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US11168353B2 (en) 2011-02-18 2021-11-09 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US12461094B2 (en) 2011-02-18 2025-11-04 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US11747327B2 (en) 2011-02-18 2023-09-05 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US12140591B2 (en) 2011-02-18 2024-11-12 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US12140590B2 (en) 2011-02-18 2024-11-12 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
US11965877B2 (en) 2011-02-18 2024-04-23 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US9222128B2 (en) 2011-03-18 2015-12-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US11788127B2 (en) 2011-04-15 2023-10-17 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US9765389B2 (en) 2011-04-15 2017-09-19 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US10781482B2 (en) 2011-04-15 2020-09-22 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
US10190115B2 (en) 2011-04-25 2019-01-29 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9885034B2 (en) 2011-04-25 2018-02-06 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US11939573B2 (en) 2011-04-25 2024-03-26 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US9347059B2 (en) 2011-04-25 2016-05-24 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US10760073B2 (en) 2011-04-25 2020-09-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
US11754499B2 (en) 2011-06-02 2023-09-12 Bio-Rad Laboratories, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US11898193B2 (en) 2011-07-20 2024-02-13 Bio-Rad Laboratories, Inc. Manipulating droplet size
US9480983B2 (en) 2011-09-30 2016-11-01 Becton, Dickinson And Company Unitized reagent strip
US10076754B2 (en) 2011-09-30 2018-09-18 Becton, Dickinson And Company Unitized reagent strip
USD831843S1 (en) 2011-09-30 2018-10-23 Becton, Dickinson And Company Single piece reagent holder
US9222954B2 (en) 2011-09-30 2015-12-29 Becton, Dickinson And Company Unitized reagent strip
USD905269S1 (en) 2011-09-30 2020-12-15 Becton, Dickinson And Company Single piece reagent holder
USD1029291S1 (en) 2011-09-30 2024-05-28 Becton, Dickinson And Company Single piece reagent holder
USD742027S1 (en) 2011-09-30 2015-10-27 Becton, Dickinson And Company Single piece reagent holder
US11453906B2 (en) 2011-11-04 2022-09-27 Handylab, Inc. Multiplexed diagnostic detection apparatus and methods
US10822644B2 (en) 2012-02-03 2020-11-03 Becton, Dickinson And Company External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US9952126B2 (en) 2012-02-29 2018-04-24 Fluidigm Corporation Methods for multiple single-cell capturing and processing using micro fluidics
US9304065B2 (en) 2012-02-29 2016-04-05 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US9506845B2 (en) 2012-02-29 2016-11-29 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US9429500B2 (en) 2012-02-29 2016-08-30 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US10578633B2 (en) 2013-03-15 2020-03-03 Fluidigm Corporation Methods and devices for analysis of defined multicellular combinations
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US11174509B2 (en) 2013-12-12 2021-11-16 Bio-Rad Laboratories, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
EP3201596A4 (en) * 2014-10-01 2018-07-11 University of Tasmania Extraction and concentration device
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions

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