UY27354A1 - Proceso para la purificación de proteínas farmacologicamente activas a traves de cromatografía de intercambio catiónico - Google Patents
Proceso para la purificación de proteínas farmacologicamente activas a traves de cromatografía de intercambio catiónicoInfo
- Publication number
- UY27354A1 UY27354A1 UY27354A UY27354A UY27354A1 UY 27354 A1 UY27354 A1 UY 27354A1 UY 27354 A UY27354 A UY 27354A UY 27354 A UY27354 A UY 27354A UY 27354 A1 UY27354 A1 UY 27354A1
- Authority
- UY
- Uruguay
- Prior art keywords
- purification
- exchange chromatography
- pharmacologically active
- proteins
- cationic exchange
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/811—Interferon
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Se basa en la utilización de cromatografía de intercambio catiónico sobre una matriz sólida, llevado a cabo a un nivel de pH, más alto con respecto al pH correspondiente al punto isoeléctrico, pl, de las proteínas a ser purificadas, siendo que a este nivel de pH, sin embargo, dichas proteínas aún permanecen absorbidas. Utilizan soluciones buffer con valores de pH de intensidad iónica que se ajustan cada tanto al tipo de proteína farmacológicamente activa a ser purificada a modo de obtener dicho resultado. El proceso se encuentra dirigido principalmente a la purificación de las proteínas interferona y albúmina.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT2001BO000426A ITBO20010426A1 (it) | 2001-07-06 | 2001-07-06 | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UY27354A1 true UY27354A1 (es) | 2003-04-30 |
Family
ID=11439471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UY27354A UY27354A1 (es) | 2001-07-06 | 2002-06-24 | Proceso para la purificación de proteínas farmacologicamente activas a traves de cromatografía de intercambio catiónico |
Country Status (27)
| Country | Link |
|---|---|
| US (1) | US6866782B2 (es) |
| EP (1) | EP1273592B1 (es) |
| JP (1) | JP3940037B2 (es) |
| KR (1) | KR100771252B1 (es) |
| CN (1) | CN100484954C (es) |
| AR (1) | AR034663A1 (es) |
| AT (1) | ATE307824T1 (es) |
| BG (1) | BG65748B1 (es) |
| BR (1) | BR0202431A (es) |
| CA (1) | CA2388716C (es) |
| CU (1) | CU23149A3 (es) |
| DE (1) | DE60206847T2 (es) |
| DK (1) | DK1273592T3 (es) |
| DZ (1) | DZ3236A1 (es) |
| EG (1) | EG23150A (es) |
| ES (1) | ES2250544T3 (es) |
| HR (1) | HRP20020480B1 (es) |
| HU (1) | HUP0202124A2 (es) |
| IT (1) | ITBO20010426A1 (es) |
| ME (1) | MEP3408A (es) |
| MX (1) | MXPA02006303A (es) |
| PL (1) | PL209954B1 (es) |
| RS (1) | RS51512B (es) |
| RU (1) | RU2274471C2 (es) |
| SI (1) | SI1273592T1 (es) |
| SK (1) | SK287827B6 (es) |
| UY (1) | UY27354A1 (es) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1628729A2 (en) | 2003-05-16 | 2006-03-01 | Cryobiophysica, Inc. | External gradient chromatofocusing |
| WO2005100379A1 (ja) * | 2004-03-30 | 2005-10-27 | Hiroshi Yanagisawa | タンパク質の分離方法 |
| US7943046B2 (en) * | 2004-10-01 | 2011-05-17 | Agilent Technologies, Inc | Methods and systems for on-column protein delipidation |
| US7449116B2 (en) * | 2004-10-01 | 2008-11-11 | Agilent Technologies, Inc. | Methods and systems for protein separation |
| HU230758B1 (hu) * | 2006-04-12 | 2018-03-28 | Crealta Pharmaceuticals LLC 100% | Fehérjék tisztítása kationos felületaktív anyaggal |
| US20100261275A1 (en) * | 2007-12-10 | 2010-10-14 | Yves Durocher | Production of Recombinant Interferon Proteins |
| DE102009032179A1 (de) | 2009-07-07 | 2011-01-13 | Biogenerix Ag | Verfahren zur Reinigung von Interferon beta |
| CN101768601B (zh) * | 2010-02-01 | 2013-08-07 | 山东泉港药业有限公司 | 一种重组人血白蛋白—干扰素α2b的生产方法 |
| SG10201605397PA (en) * | 2011-07-01 | 2016-08-30 | Hoffmann La Roche | Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides |
| US8921113B2 (en) | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
| WO2014137903A2 (en) | 2013-03-08 | 2014-09-12 | Genzyme Corporation | Integrated continuous manufacturing of therapeutic protein drug substances |
| CN104236983A (zh) * | 2013-06-14 | 2014-12-24 | 中国科学院大连化学物理研究所 | 溶液样品中含长烷基链的离子液体的去除方法 |
| TWI671312B (zh) | 2014-01-17 | 2019-09-11 | 美商健臻公司 | 無菌層析法及製法 |
| TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
| WO2016079598A1 (en) * | 2014-11-18 | 2016-05-26 | Unichem Laboratories Limited | An improved process for the preparation of pharmacopoeial grade interferon alpha 2b |
| CN106496302B (zh) * | 2015-09-08 | 2021-12-10 | 三生国健药业（上海）股份有限公司 | 一种用离子交换层析纯化蛋白质的方法 |
| GB201517450D0 (en) * | 2015-10-02 | 2015-11-18 | Ipsen Biopharm Ltd | Method |
| CN108367245A (zh) * | 2015-12-09 | 2018-08-03 | 巴斯夫欧洲公司 | 在解吸条件下从发酵固体纯化蛋白质的方法 |
| GB2568936B (en) | 2017-12-01 | 2019-12-25 | Ford Global Tech Llc | Liquid cooled injector |
| US11369703B2 (en) | 2018-08-31 | 2022-06-28 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
| US11022585B2 (en) * | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
| CN111138523A (zh) * | 2019-12-10 | 2020-05-12 | 天津生机集团股份有限公司 | 一种从重组鸡干扰素α复性液中纯化制备重组鸡干扰素α的方法 |
| CN113968900B (zh) * | 2020-07-23 | 2023-07-25 | 中元汇吉生物技术股份有限公司 | 一种纯化C1q蛋白的方法 |
| CN115918902A (zh) * | 2022-12-19 | 2023-04-07 | 新疆农业科学院农业质量标准与检测技术研究所 | 一种通过微水波扩散重力法预处理葡萄脱水并获取高酚葡萄提取物的方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4228154A (en) * | 1979-02-26 | 1980-10-14 | Armour Pharmaceutical Company | Purification of plasma albumin by ion exchange chromatography |
| US4534906A (en) * | 1982-11-01 | 1985-08-13 | Genentech, Inc. | Removal of impurities from human leukocyte interferon preparations |
| DE3515336C2 (de) * | 1985-04-27 | 1994-01-20 | Boehringer Ingelheim Int | Verfahren zur Herstellung und Reinigung von â-Interferon |
| US5037644A (en) * | 1986-10-27 | 1991-08-06 | Cetus Corporation | Pharmaceutical compositions of recombinant interleukin-2 and formulation processes |
| US4732683A (en) * | 1986-12-02 | 1988-03-22 | Biospectrum, Inc. | Purification method for alpha interferon |
| US4765903A (en) * | 1987-10-06 | 1988-08-23 | Interferon Sciences, Inc. | Purification of monomeric interferon |
| US5250662A (en) * | 1989-10-05 | 1993-10-05 | Alpha Therapeutic Corporation | Albumin purification |
| US5849874A (en) * | 1991-07-12 | 1998-12-15 | Gist-Brocades, N.V. | Process for the purification of serum albumin |
| IT1262899B (it) * | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| US5440018A (en) * | 1992-05-20 | 1995-08-08 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
| US5521287A (en) * | 1992-05-20 | 1996-05-28 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
| US5728553A (en) * | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
| US5367054A (en) * | 1993-04-12 | 1994-11-22 | Stolle Research & Development Corp. | Large-scale purification of egg immunoglobulin |
| DK0679718T3 (da) * | 1994-04-09 | 2001-10-22 | Hoffmann La Roche | Fremgangsmåde til fremstilling af alfa-interferon |
| JP3702474B2 (ja) * | 1994-06-01 | 2005-10-05 | 三菱ウェルファーマ株式会社 | 血清アルブミン製剤の製造方法 |
| US5486470A (en) * | 1994-07-21 | 1996-01-23 | Merck & Co., Inc. | Purified herpes simplex virus protease and methods of purification |
| DE69532492T2 (de) | 1994-08-31 | 2004-12-02 | Mitsubishi Pharma Corp. | Verfahren zur Reinigung von rekombinantem menschlichem Serumalbumin |
| EP0871671A1 (en) * | 1995-09-07 | 1998-10-21 | PPL Therapeutics (Scotland) Limited | Purification of alpha-1 proteinase inhibitor |
| GB9902000D0 (en) | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
-
2001
- 2001-07-06 IT IT2001BO000426A patent/ITBO20010426A1/it unknown
-
2002
- 2002-05-31 HR HR20020480A patent/HRP20020480B1/xx not_active IP Right Cessation
- 2002-06-03 BG BG106768A patent/BG65748B1/bg unknown
- 2002-06-03 CA CA002388716A patent/CA2388716C/en not_active Expired - Fee Related
- 2002-06-06 CU CU112A patent/CU23149A3/es not_active IP Right Cessation
- 2002-06-10 US US10/166,459 patent/US6866782B2/en not_active Expired - Fee Related
- 2002-06-14 SK SK859-2002A patent/SK287827B6/sk not_active IP Right Cessation
- 2002-06-19 AT AT02013555T patent/ATE307824T1/de active
- 2002-06-19 SI SI200230227T patent/SI1273592T1/sl unknown
- 2002-06-19 ES ES02013555T patent/ES2250544T3/es not_active Expired - Lifetime
- 2002-06-19 DK DK02013555T patent/DK1273592T3/da active
- 2002-06-19 DE DE60206847T patent/DE60206847T2/de not_active Expired - Lifetime
- 2002-06-19 EP EP02013555A patent/EP1273592B1/en not_active Expired - Lifetime
- 2002-06-24 UY UY27354A patent/UY27354A1/es not_active Application Discontinuation
- 2002-06-24 MX MXPA02006303A patent/MXPA02006303A/es active IP Right Grant
- 2002-06-26 RS YUP-505/02A patent/RS51512B/sr unknown
- 2002-06-26 ME MEP-34/08A patent/MEP3408A/xx unknown
- 2002-06-26 JP JP2002186407A patent/JP3940037B2/ja not_active Expired - Fee Related
- 2002-06-26 DZ DZ020153A patent/DZ3236A1/fr active
- 2002-06-27 PL PL354769A patent/PL209954B1/pl unknown
- 2002-06-27 BR BR0202431-4A patent/BR0202431A/pt not_active Application Discontinuation
- 2002-06-28 HU HU0202124A patent/HUP0202124A2/hu not_active Application Discontinuation
- 2002-06-28 CN CNB021402175A patent/CN100484954C/zh not_active Expired - Fee Related
- 2002-06-28 AR ARP020102459A patent/AR034663A1/es active IP Right Grant
- 2002-06-28 RU RU2002117383/15A patent/RU2274471C2/ru not_active IP Right Cessation
- 2002-06-29 EG EG2002060743A patent/EG23150A/xx active
- 2002-06-29 KR KR1020020037622A patent/KR100771252B1/ko not_active IP Right Cessation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 109 | Application deemed to be withdrawn |
Effective date: 20141217 |