ES2098379T3 - Procedimiento para la reactivacion de una proteina desnaturalizada. - Google Patents

Procedimiento para la reactivacion de una proteina desnaturalizada.

Info

Publication number
ES2098379T3
ES2098379T3 ES91919945T ES91919945T ES2098379T3 ES 2098379 T3 ES2098379 T3 ES 2098379T3 ES 91919945 T ES91919945 T ES 91919945T ES 91919945 T ES91919945 T ES 91919945T ES 2098379 T3 ES2098379 T3 ES 2098379T3
Authority
ES
Spain
Prior art keywords
pct
protein
reactivation
procedure
sec
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91919945T
Other languages
English (en)
Inventor
Dorothea Ambrosius
Rainer Rudolph
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2098379T3 publication Critical patent/ES2098379T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL INVENTO DESCRIBE UN PROCESO PARA LA REACTIVACION DE PROTEINA DESNATURALIZADA EN EL QUE LA PROTEINA ES INCUBADA CON UNA SOLUCION DE TRI-BASE Y/O UNA TRI-SAL EN UNA CONCENTRACION DE AL MENOS 400 MOLECULAS POR LITRO Y A UN VALOR DE PH EN EL QUE LA PROTEINA TRATADA PUEDE ASUMIR SU CONFORMACION NATURAL
ES91919945T 1990-11-22 1991-11-21 Procedimiento para la reactivacion de una proteina desnaturalizada. Expired - Lifetime ES2098379T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4037196A DE4037196A1 (de) 1990-11-22 1990-11-22 Verfahren zur reaktivierung von denaturiertem protein

Publications (1)

Publication Number Publication Date
ES2098379T3 true ES2098379T3 (es) 1997-05-01

Family

ID=6418742

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91919945T Expired - Lifetime ES2098379T3 (es) 1990-11-22 1991-11-21 Procedimiento para la reactivacion de una proteina desnaturalizada.

Country Status (17)

Country Link
US (1) US5618927A (es)
EP (1) EP0512087B1 (es)
JP (1) JP2572517B2 (es)
KR (1) KR960013385B1 (es)
AT (1) ATE149514T1 (es)
AU (1) AU8919591A (es)
CA (1) CA2074182C (es)
DE (2) DE4037196A1 (es)
DK (1) DK0512087T3 (es)
ES (1) ES2098379T3 (es)
GR (1) GR3023110T3 (es)
HU (1) HU213589B (es)
IE (1) IE62052B1 (es)
PT (1) PT99578B (es)
RU (1) RU2070888C1 (es)
WO (1) WO1992009622A1 (es)
ZA (1) ZA919207B (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL109149A0 (en) * 1993-04-01 1994-06-24 Schering Corp In vitro refolding of recombinant fv fragments
US20030133905A1 (en) * 2001-12-20 2003-07-17 Zhenze Hu Composition for treating contact lenses in the eye
SI21273A (sl) * 2002-07-31 2004-02-29 LEK farmacevtska dru�ba d.d. Priprava inkluzijskih teles z visokim deležem pravilno zvitega prekurzorja heterolognega proteina
CN101506222B (zh) * 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
WO2013168178A1 (en) * 2012-03-30 2013-11-14 Krishnan Archana Rajesh Process for renaturation of polypeptides
US20160039868A1 (en) * 2013-03-22 2016-02-11 Biogenomics Limited Process for isolation and stabilisation of key intermediates for high efficiency refolding of recombinant proteins
RU2646103C2 (ru) * 2016-03-30 2018-03-01 Федеральное бюджетное учреждение науки Государственный научный центр прикладной микробиологии и биотехнологии (ФБУН ГНЦ ПМБ) Способ выделения ренатурированного белка g из маркированного полиакриламидного геля

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
DE3903581A1 (de) * 1989-02-07 1990-08-16 Boehringer Mannheim Gmbh Gewebs-plasminogenaktivator-derivat

Also Published As

Publication number Publication date
DK0512087T3 (da) 1997-08-25
KR960013385B1 (ko) 1996-10-04
EP0512087A1 (de) 1992-11-11
CA2074182A1 (en) 1992-05-23
WO1992009622A1 (de) 1992-06-11
PT99578B (pt) 1999-04-30
HU9202354D0 (en) 1992-10-28
JPH05502245A (ja) 1993-04-22
IE62052B1 (en) 1994-12-14
HUT63437A (en) 1993-08-30
DE4037196A1 (de) 1992-05-27
RU2070888C1 (ru) 1996-12-27
IE913474A1 (en) 1992-06-03
CA2074182C (en) 1999-10-05
AU8919591A (en) 1992-06-25
DE59108583D1 (de) 1997-04-10
KR927003623A (ko) 1992-12-18
JP2572517B2 (ja) 1997-01-16
EP0512087B1 (de) 1997-03-05
HU213589B (en) 1997-08-28
GR3023110T3 (en) 1997-07-30
PT99578A (pt) 1992-10-30
ATE149514T1 (de) 1997-03-15
ZA919207B (en) 1992-08-26
US5618927A (en) 1997-04-08

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