YU50502A - Postupak za prečišćavanje farmakološki aktivnih proteina pomoću katjonske izmenjivačke hromatografije - Google Patents
Postupak za prečišćavanje farmakološki aktivnih proteina pomoću katjonske izmenjivačke hromatografijeInfo
- Publication number
- YU50502A YU50502A YU50502A YUP50502A YU50502A YU 50502 A YU50502 A YU 50502A YU 50502 A YU50502 A YU 50502A YU P50502 A YUP50502 A YU P50502A YU 50502 A YU50502 A YU 50502A
- Authority
- YU
- Yugoslavia
- Prior art keywords
- purification
- active proteins
- pharmacollogically
- exchange chromatography
- cationic exchange
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/811—Interferon
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Predmet pronalaska je postupak za prečišćavanje farmakološki aktivnih proteina, zasnovanih na upotrebi katjonske izmenjivačke hromatografije na čvrstom nosaču izvodjenom na baznim pH, tj. višim, u odnosu na pH koji odgovara izoelektričnoj tačci, pl, proteina koji se prečišćavaju, pH na kojem su medjutim pomenuti proteini još uvek zaostali absorbovani. Rastvori pufera sa vrednošću pH i jonskom jačinom podešenom sa vremena na vreme vrsti farmakološki aktivnih proteina koji se prečišćavaju se koristi u cilju dobijanja takvih rezultata. Postupak se uglavnom odnosi na prečišćavanje interferona i albumin proteina.[The subject matter of the invention is a procedure of purification of pharmacollogically active proteins, through the use of cationic exchange chromatography on the solid carrier done on the primary pH, i.e. higher, in relation to pH appropriate to the electric point, pI protein that are purified, pH whereon the mentioned proteins although residual yet get absorbed. The solvents of buffer with pH and ionic strength adjusted from time to time to the type of pharmacologically active proteins that are purified are used for preparation of such results. The procedure mainly relates to purification of interferon and albumins.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MEP-34/08A MEP3408A (en) | 2001-07-06 | 2002-06-26 | Process for the purification of pharmacologically active proteins through cationic exchange chromatography |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT2001BO000426A ITBO20010426A1 (it) | 2001-07-06 | 2001-07-06 | Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico |
Publications (2)
Publication Number | Publication Date |
---|---|
YU50502A true YU50502A (sh) | 2005-09-19 |
RS51512B RS51512B (sr) | 2011-06-30 |
Family
ID=11439471
Country Status (27)
Country | Link |
---|---|
US (1) | US6866782B2 (sh) |
EP (1) | EP1273592B1 (sh) |
JP (1) | JP3940037B2 (sh) |
KR (1) | KR100771252B1 (sh) |
CN (1) | CN100484954C (sh) |
AR (1) | AR034663A1 (sh) |
AT (1) | ATE307824T1 (sh) |
BG (1) | BG65748B1 (sh) |
BR (1) | BR0202431A (sh) |
CA (1) | CA2388716C (sh) |
CU (1) | CU23149A3 (sh) |
DE (1) | DE60206847T2 (sh) |
DK (1) | DK1273592T3 (sh) |
DZ (1) | DZ3236A1 (sh) |
EG (1) | EG23150A (sh) |
ES (1) | ES2250544T3 (sh) |
HR (1) | HRP20020480B1 (sh) |
HU (1) | HUP0202124A2 (sh) |
IT (1) | ITBO20010426A1 (sh) |
ME (1) | MEP3408A (sh) |
MX (1) | MXPA02006303A (sh) |
PL (1) | PL209954B1 (sh) |
RS (1) | RS51512B (sh) |
RU (1) | RU2274471C2 (sh) |
SI (1) | SI1273592T1 (sh) |
SK (1) | SK287827B6 (sh) |
UY (1) | UY27354A1 (sh) |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7790025B2 (en) | 2003-05-16 | 2010-09-07 | Cryobiophysica, Inc. | External gradient chromatofocusing |
EP1739089A4 (en) * | 2004-03-30 | 2007-05-02 | Hiroshi Yanagisawa | PROCESS FOR DISINTER SEPARATION |
US7943046B2 (en) * | 2004-10-01 | 2011-05-17 | Agilent Technologies, Inc | Methods and systems for on-column protein delipidation |
US7449116B2 (en) * | 2004-10-01 | 2008-11-11 | Agilent Technologies, Inc. | Methods and systems for protein separation |
US9534013B2 (en) * | 2006-04-12 | 2017-01-03 | Horizon Pharma Rheumatology Llc | Purification of proteins with cationic surfactant |
US20100261275A1 (en) * | 2007-12-10 | 2010-10-14 | Yves Durocher | Production of Recombinant Interferon Proteins |
DE102009032179A1 (de) | 2009-07-07 | 2011-01-13 | Biogenerix Ag | Verfahren zur Reinigung von Interferon beta |
CN101768601B (zh) * | 2010-02-01 | 2013-08-07 | 山东泉港药业有限公司 | 一种重组人血白蛋白—干扰素α2b的生产方法 |
SG10201605397PA (en) * | 2011-07-01 | 2016-08-30 | Hoffmann La Roche | Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides |
US8921113B2 (en) | 2012-12-21 | 2014-12-30 | Dionex Corporation | Buffer kit and method of generating a linear pH gradient |
SG11201506775VA (en) * | 2013-03-08 | 2015-09-29 | Genzyme Corp | Continuous purification of therapeutic proteins |
CN104236983A (zh) * | 2013-06-14 | 2014-12-24 | 中国科学院大连化学物理研究所 | 溶液样品中含长烷基链的离子液体的去除方法 |
TWI709570B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析法及製法 |
TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
EP3221450A1 (en) * | 2014-11-18 | 2017-09-27 | Unichem Laboratories Limited | An improved process for the preparation of pharmacopoeial grade interferon alpha 2b |
CN106496302B (zh) * | 2015-09-08 | 2021-12-10 | 三生国健药业(上海)股份有限公司 | 一种用离子交换层析纯化蛋白质的方法 |
GB201517450D0 (en) * | 2015-10-02 | 2015-11-18 | Ipsen Biopharm Ltd | Method |
JP2018536418A (ja) * | 2015-12-09 | 2018-12-13 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | 脱離条件下で発酵固体からタンパク質を精製する方法 |
GB2568936B (en) | 2017-12-01 | 2019-12-25 | Ford Global Tech Llc | Liquid cooled injector |
AU2019332764A1 (en) | 2018-08-31 | 2021-05-06 | Genzyme Corporation | Sterile chromatography resin and use thereof in manufacturing processes |
US11022585B2 (en) | 2019-06-09 | 2021-06-01 | Dionex Corporation | Methods and systems for optimizing buffer conditions with liquid chromatography |
CN111138523A (zh) * | 2019-12-10 | 2020-05-12 | 天津生机集团股份有限公司 | 一种从重组鸡干扰素α复性液中纯化制备重组鸡干扰素α的方法 |
CN113968900B (zh) * | 2020-07-23 | 2023-07-25 | 中元汇吉生物技术股份有限公司 | 一种纯化C1q蛋白的方法 |
CN115918902A (zh) * | 2022-12-19 | 2023-04-07 | 新疆农业科学院农业质量标准与检测技术研究所 | 一种通过微水波扩散重力法预处理葡萄脱水并获取高酚葡萄提取物的方法 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4228154A (en) * | 1979-02-26 | 1980-10-14 | Armour Pharmaceutical Company | Purification of plasma albumin by ion exchange chromatography |
US4534906A (en) * | 1982-11-01 | 1985-08-13 | Genentech, Inc. | Removal of impurities from human leukocyte interferon preparations |
DE3515336C2 (de) * | 1985-04-27 | 1994-01-20 | Boehringer Ingelheim Int | Verfahren zur Herstellung und Reinigung von â-Interferon |
US5037644A (en) * | 1986-10-27 | 1991-08-06 | Cetus Corporation | Pharmaceutical compositions of recombinant interleukin-2 and formulation processes |
US4732683A (en) * | 1986-12-02 | 1988-03-22 | Biospectrum, Inc. | Purification method for alpha interferon |
US4765903A (en) * | 1987-10-06 | 1988-08-23 | Interferon Sciences, Inc. | Purification of monomeric interferon |
US5250662A (en) * | 1989-10-05 | 1993-10-05 | Alpha Therapeutic Corporation | Albumin purification |
US5849874A (en) * | 1991-07-12 | 1998-12-15 | Gist-Brocades, N.V. | Process for the purification of serum albumin |
IT1262899B (it) * | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
US5440018A (en) * | 1992-05-20 | 1995-08-08 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
US5521287A (en) * | 1992-05-20 | 1996-05-28 | The Green Cross Corporation | Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same |
US5728553A (en) * | 1992-09-23 | 1998-03-17 | Delta Biotechnology Limited | High purity albumin and method of producing |
US5367054A (en) * | 1993-04-12 | 1994-11-22 | Stolle Research & Development Corp. | Large-scale purification of egg immunoglobulin |
ES2161793T3 (es) * | 1994-04-09 | 2001-12-16 | Hoffmann La Roche | Proceso para la produccion de alfa-interferona. |
JP3702474B2 (ja) * | 1994-06-01 | 2005-10-05 | 三菱ウェルファーマ株式会社 | 血清アルブミン製剤の製造方法 |
US5486470A (en) * | 1994-07-21 | 1996-01-23 | Merck & Co., Inc. | Purified herpes simplex virus protease and methods of purification |
CA2157219C (en) | 1994-08-31 | 2010-10-05 | Munehiro Noda | Process for purifying recombinant human serum albumin |
AU6884396A (en) * | 1995-09-07 | 1997-03-27 | Ppl Therapeutics (Scotland) Ltd | Purification of alpha-1 proteinase inhibitor |
GB9902000D0 (en) * | 1999-01-30 | 1999-03-17 | Delta Biotechnology Ltd | Process |
-
2001
- 2001-07-06 IT IT2001BO000426A patent/ITBO20010426A1/it unknown
-
2002
- 2002-05-31 HR HR20020480A patent/HRP20020480B1/xx not_active IP Right Cessation
- 2002-06-03 BG BG106768A patent/BG65748B1/bg unknown
- 2002-06-03 CA CA002388716A patent/CA2388716C/en not_active Expired - Fee Related
- 2002-06-06 CU CU112A patent/CU23149A3/es not_active IP Right Cessation
- 2002-06-10 US US10/166,459 patent/US6866782B2/en not_active Expired - Fee Related
- 2002-06-14 SK SK859-2002A patent/SK287827B6/sk not_active IP Right Cessation
- 2002-06-19 DK DK02013555T patent/DK1273592T3/da active
- 2002-06-19 EP EP02013555A patent/EP1273592B1/en not_active Expired - Lifetime
- 2002-06-19 SI SI200230227T patent/SI1273592T1/sl unknown
- 2002-06-19 AT AT02013555T patent/ATE307824T1/de active
- 2002-06-19 ES ES02013555T patent/ES2250544T3/es not_active Expired - Lifetime
- 2002-06-19 DE DE60206847T patent/DE60206847T2/de not_active Expired - Lifetime
- 2002-06-24 MX MXPA02006303A patent/MXPA02006303A/es active IP Right Grant
- 2002-06-24 UY UY27354A patent/UY27354A1/es not_active Application Discontinuation
- 2002-06-26 RS YUP-505/02A patent/RS51512B/sr unknown
- 2002-06-26 ME MEP-34/08A patent/MEP3408A/xx unknown
- 2002-06-26 JP JP2002186407A patent/JP3940037B2/ja not_active Expired - Fee Related
- 2002-06-26 DZ DZ020153A patent/DZ3236A1/fr active
- 2002-06-27 BR BR0202431-4A patent/BR0202431A/pt not_active Application Discontinuation
- 2002-06-27 PL PL354769A patent/PL209954B1/pl unknown
- 2002-06-28 HU HU0202124A patent/HUP0202124A2/hu not_active Application Discontinuation
- 2002-06-28 RU RU2002117383/15A patent/RU2274471C2/ru not_active IP Right Cessation
- 2002-06-28 AR ARP020102459A patent/AR034663A1/es active IP Right Grant
- 2002-06-28 CN CNB021402175A patent/CN100484954C/zh not_active Expired - Fee Related
- 2002-06-29 EG EG2002060743A patent/EG23150A/xx active
- 2002-06-29 KR KR1020020037622A patent/KR100771252B1/ko not_active IP Right Cessation
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