YU50502A - Postupak za prečišćavanje farmakološki aktivnih proteina pomoću katjonske izmenjivačke hromatografije - Google Patents

Postupak za prečišćavanje farmakološki aktivnih proteina pomoću katjonske izmenjivačke hromatografije

Info

Publication number
YU50502A
YU50502A YU50502A YUP50502A YU50502A YU 50502 A YU50502 A YU 50502A YU 50502 A YU50502 A YU 50502A YU P50502 A YUP50502 A YU P50502A YU 50502 A YU50502 A YU 50502A
Authority
YU
Yugoslavia
Prior art keywords
purification
active proteins
pharmacollogically
exchange chromatography
cationic exchange
Prior art date
Application number
YU50502A
Other languages
English (en)
Inventor
Lucia Scapol
Viscomi Giuseppe Claudio Ot
Original Assignee
Alfa Wassermann S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann S.P.A. filed Critical Alfa Wassermann S.P.A.
Priority to MEP-34/08A priority Critical patent/MEP3408A/xx
Publication of YU50502A publication Critical patent/YU50502A/sh
Publication of RS51512B publication Critical patent/RS51512B/sr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/811—Interferon

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Predmet pronalaska je postupak za prečišćavanje farmakološki aktivnih proteina, zasnovanih na upotrebi katjonske izmenjivačke hromatografije na čvrstom nosaču izvodjenom na baznim pH, tj. višim, u odnosu na pH koji odgovara izoelektričnoj tačci, pl, proteina koji se prečišćavaju, pH na kojem su medjutim pomenuti proteini još uvek zaostali absorbovani. Rastvori pufera sa vrednošću pH i jonskom jačinom podešenom sa vremena na vreme vrsti farmakološki aktivnih proteina koji se prečišćavaju se koristi u cilju dobijanja takvih rezultata. Postupak se uglavnom odnosi na prečišćavanje interferona i albumin proteina.[The subject matter of the invention is a procedure of purification of pharmacollogically active proteins, through the use of cationic exchange chromatography on the solid carrier done on the primary pH, i.e. higher, in relation to pH appropriate to the electric point, pI protein that are purified, pH whereon the mentioned proteins although residual yet get absorbed. The solvents of buffer with pH and ionic strength adjusted from time to time to the type of pharmacologically active proteins that are purified are used for preparation of such results. The procedure mainly relates to purification of interferon and albumins.
YUP-505/02A 2001-07-06 2002-06-26 Postupak za prečišćavanje interferonskih proteina pomoću katjonske izmenjivačke hromatografije RS51512B (sr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MEP-34/08A MEP3408A (en) 2001-07-06 2002-06-26 Process for the purification of pharmacologically active proteins through cationic exchange chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2001BO000426A ITBO20010426A1 (it) 2001-07-06 2001-07-06 Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico

Publications (2)

Publication Number Publication Date
YU50502A true YU50502A (sh) 2005-09-19
RS51512B RS51512B (sr) 2011-06-30

Family

ID=11439471

Country Status (27)

Country Link
US (1) US6866782B2 (sh)
EP (1) EP1273592B1 (sh)
JP (1) JP3940037B2 (sh)
KR (1) KR100771252B1 (sh)
CN (1) CN100484954C (sh)
AR (1) AR034663A1 (sh)
AT (1) ATE307824T1 (sh)
BG (1) BG65748B1 (sh)
BR (1) BR0202431A (sh)
CA (1) CA2388716C (sh)
CU (1) CU23149A3 (sh)
DE (1) DE60206847T2 (sh)
DK (1) DK1273592T3 (sh)
DZ (1) DZ3236A1 (sh)
EG (1) EG23150A (sh)
ES (1) ES2250544T3 (sh)
HR (1) HRP20020480B1 (sh)
HU (1) HUP0202124A2 (sh)
IT (1) ITBO20010426A1 (sh)
ME (1) MEP3408A (sh)
MX (1) MXPA02006303A (sh)
PL (1) PL209954B1 (sh)
RS (1) RS51512B (sh)
RU (1) RU2274471C2 (sh)
SI (1) SI1273592T1 (sh)
SK (1) SK287827B6 (sh)
UY (1) UY27354A1 (sh)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7790025B2 (en) 2003-05-16 2010-09-07 Cryobiophysica, Inc. External gradient chromatofocusing
EP1739089A4 (en) * 2004-03-30 2007-05-02 Hiroshi Yanagisawa PROCESS FOR DISINTER SEPARATION
US7943046B2 (en) * 2004-10-01 2011-05-17 Agilent Technologies, Inc Methods and systems for on-column protein delipidation
US7449116B2 (en) * 2004-10-01 2008-11-11 Agilent Technologies, Inc. Methods and systems for protein separation
US9534013B2 (en) * 2006-04-12 2017-01-03 Horizon Pharma Rheumatology Llc Purification of proteins with cationic surfactant
US20100261275A1 (en) * 2007-12-10 2010-10-14 Yves Durocher Production of Recombinant Interferon Proteins
DE102009032179A1 (de) 2009-07-07 2011-01-13 Biogenerix Ag Verfahren zur Reinigung von Interferon beta
CN101768601B (zh) * 2010-02-01 2013-08-07 山东泉港药业有限公司 一种重组人血白蛋白—干扰素α2b的生产方法
SG10201605397PA (en) * 2011-07-01 2016-08-30 Hoffmann La Roche Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides
US8921113B2 (en) 2012-12-21 2014-12-30 Dionex Corporation Buffer kit and method of generating a linear pH gradient
SG11201506775VA (en) * 2013-03-08 2015-09-29 Genzyme Corp Continuous purification of therapeutic proteins
CN104236983A (zh) * 2013-06-14 2014-12-24 中国科学院大连化学物理研究所 溶液样品中含长烷基链的离子液体的去除方法
TWI709570B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析法及製法
TWI709569B (zh) 2014-01-17 2020-11-11 美商健臻公司 無菌層析樹脂及其用於製造方法的用途
EP3221450A1 (en) * 2014-11-18 2017-09-27 Unichem Laboratories Limited An improved process for the preparation of pharmacopoeial grade interferon alpha 2b
CN106496302B (zh) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 一种用离子交换层析纯化蛋白质的方法
GB201517450D0 (en) * 2015-10-02 2015-11-18 Ipsen Biopharm Ltd Method
JP2018536418A (ja) * 2015-12-09 2018-12-13 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 脱離条件下で発酵固体からタンパク質を精製する方法
GB2568936B (en) 2017-12-01 2019-12-25 Ford Global Tech Llc Liquid cooled injector
AU2019332764A1 (en) 2018-08-31 2021-05-06 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
US11022585B2 (en) 2019-06-09 2021-06-01 Dionex Corporation Methods and systems for optimizing buffer conditions with liquid chromatography
CN111138523A (zh) * 2019-12-10 2020-05-12 天津生机集团股份有限公司 一种从重组鸡干扰素α复性液中纯化制备重组鸡干扰素α的方法
CN113968900B (zh) * 2020-07-23 2023-07-25 中元汇吉生物技术股份有限公司 一种纯化C1q蛋白的方法
CN115918902A (zh) * 2022-12-19 2023-04-07 新疆农业科学院农业质量标准与检测技术研究所 一种通过微水波扩散重力法预处理葡萄脱水并获取高酚葡萄提取物的方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228154A (en) * 1979-02-26 1980-10-14 Armour Pharmaceutical Company Purification of plasma albumin by ion exchange chromatography
US4534906A (en) * 1982-11-01 1985-08-13 Genentech, Inc. Removal of impurities from human leukocyte interferon preparations
DE3515336C2 (de) * 1985-04-27 1994-01-20 Boehringer Ingelheim Int Verfahren zur Herstellung und Reinigung von â-Interferon
US5037644A (en) * 1986-10-27 1991-08-06 Cetus Corporation Pharmaceutical compositions of recombinant interleukin-2 and formulation processes
US4732683A (en) * 1986-12-02 1988-03-22 Biospectrum, Inc. Purification method for alpha interferon
US4765903A (en) * 1987-10-06 1988-08-23 Interferon Sciences, Inc. Purification of monomeric interferon
US5250662A (en) * 1989-10-05 1993-10-05 Alpha Therapeutic Corporation Albumin purification
US5849874A (en) * 1991-07-12 1998-12-15 Gist-Brocades, N.V. Process for the purification of serum albumin
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5440018A (en) * 1992-05-20 1995-08-08 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
US5521287A (en) * 1992-05-20 1996-05-28 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
US5728553A (en) * 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
US5367054A (en) * 1993-04-12 1994-11-22 Stolle Research & Development Corp. Large-scale purification of egg immunoglobulin
ES2161793T3 (es) * 1994-04-09 2001-12-16 Hoffmann La Roche Proceso para la produccion de alfa-interferona.
JP3702474B2 (ja) * 1994-06-01 2005-10-05 三菱ウェルファーマ株式会社 血清アルブミン製剤の製造方法
US5486470A (en) * 1994-07-21 1996-01-23 Merck & Co., Inc. Purified herpes simplex virus protease and methods of purification
CA2157219C (en) 1994-08-31 2010-10-05 Munehiro Noda Process for purifying recombinant human serum albumin
AU6884396A (en) * 1995-09-07 1997-03-27 Ppl Therapeutics (Scotland) Ltd Purification of alpha-1 proteinase inhibitor
GB9902000D0 (en) * 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process

Also Published As

Publication number Publication date
SI1273592T1 (sl) 2006-02-28
JP2003096092A (ja) 2003-04-03
CN100484954C (zh) 2009-05-06
RU2002117383A (ru) 2004-01-27
MXPA02006303A (es) 2003-02-10
KR100771252B1 (ko) 2007-10-30
BG106768A (en) 2003-05-30
BR0202431A (pt) 2003-09-09
CA2388716A1 (en) 2003-01-06
EP1273592A2 (en) 2003-01-08
US20030010715A1 (en) 2003-01-16
DE60206847T2 (de) 2006-07-06
DK1273592T3 (da) 2006-01-02
CN1396176A (zh) 2003-02-12
CA2388716C (en) 2008-01-08
HRP20020480B1 (en) 2006-11-30
SK8592002A3 (en) 2003-01-09
EG23150A (en) 2004-05-31
EP1273592A3 (en) 2003-03-19
ITBO20010426A1 (it) 2003-01-06
ITBO20010426A0 (it) 2001-07-06
PL354769A1 (en) 2003-01-13
ATE307824T1 (de) 2005-11-15
HUP0202124A2 (hu) 2003-10-28
SK287827B6 (sk) 2011-11-04
KR20030005005A (ko) 2003-01-15
DE60206847D1 (de) 2005-12-01
US6866782B2 (en) 2005-03-15
HU0202124D0 (sh) 2002-09-28
PL209954B1 (pl) 2011-11-30
RS51512B (sr) 2011-06-30
CU23149A3 (es) 2006-06-29
ES2250544T3 (es) 2006-04-16
RU2274471C2 (ru) 2006-04-20
HRP20020480A2 (en) 2003-02-28
DZ3236A1 (fr) 2005-04-20
BG65748B1 (bg) 2009-09-30
UY27354A1 (es) 2003-04-30
EP1273592B1 (en) 2005-10-26
MEP3408A (en) 2010-10-10
JP3940037B2 (ja) 2007-07-04
AR034663A1 (es) 2004-03-03

Similar Documents

Publication Publication Date Title
YU50502A (sh) Postupak za prečišćavanje farmakološki aktivnih proteina pomoću katjonske izmenjivačke hromatografije
AU2003262886A1 (en) Modified transferrin fusion proteins comprising duplicate transferrin amino or carboxy terminal domains
BG100974A (en) Vaccines against papillomavirus
AU3262497A (en) Use of pyridyl alkane, pyridyl alkene and/or pyridyl alkine acid amides in the treatment of tumors or for immunosuppression
EP1200124A4 (en) IMMUNOGLOBIN FUSION PROTEINS
BR0212256A (pt) Proteìnas de fusão de transferrina modificadas
DK1025124T3 (da) Human interferon-alfa-analoge med lav toksicitet
MY118591A (en) Improved methods for processing activated protein c
ES2149149T1 (es) Proteinas de fijacion de interleuquina-18, su preparacion y utilizacion.
HK1036994A1 (en) Hybrid protein for inhibiting the degranulation of mastocytes and the use thereof
ATE234108T1 (de) Therapeutikum zur unterdrückung von schnarchgeräuschen
EP1939293A3 (en) Interferon-Like Protein ZCYTO21
MEP3708A (en) Pharmaceutical compositions from beard lichen (usnea barbata) and st. john's wort (hypericum perforatum) and their use
GB2367061A (en) Peptides
MY125980A (en) Highly purified cytokine activating factor and methods of use
FI930626A (fi) Proteinstruktur hos vaexttoxingelonin
MXPA04002103A (es) Una proteina de union a caspasa-8, su preparacion y uso.
PT840746E (pt) Metodo para purificacao de proteinas
ATE272072T1 (de) Verfahren zur reinigung von menschlichem interleukin-1 rezeptor antagonist aus rekombinanten e.coli
WO2004058811A3 (en) Crystal structures of yhhf polypeptides
WO2004058812A3 (en) Ribose-phosphate pyrophosphokinase polypeptides and structures
WO2003087352A3 (en) Enolase polypeptides and structures