USRE33047E - Process for producing a high-purity maltose - Google Patents

Process for producing a high-purity maltose Download PDF

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Publication number
USRE33047E
USRE33047E US07/202,249 US20224988A USRE33047E US RE33047 E USRE33047 E US RE33047E US 20224988 A US20224988 A US 20224988A US RE33047 E USRE33047 E US RE33047E
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Prior art keywords
maltose
fraction
column
glucose
starch sugar
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US07/202,249
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Toshio Miyake
Shuzo Sakai
Takashi Shibuya
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Hayashibara Seibutsu Kagaku Kenkyujo KK
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Hayashibara Seibutsu Kagaku Kenkyujo KK
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/04—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K7/00—Maltose

Definitions

  • the present invention relates to a process for producing a high-purity maltose.
  • maltose has been available as a saccharified starch product with a maltose content of about 40-50 w/w % based upon the weight of the dry solid solute (all percentages are used in the specification mean "weight percentages on dry solid basis” unless otherwise specified) which is obtainable by subjecting a liquefied starch solution to the action of a malt enzyme.
  • a starch sugar solution containing maltose is passed through a column of an anion exchange resin.
  • Japanese Patent Publication No. 46,290/77 discloses a process for producing a high-purity maltose comprising preparing a starch sugar solution substantially consisting of dextrin and about 65% maltose, and applying the solution to an anion exchange resin of OH-form to adsorb the maltose constituent and also to remove the dextrin constituent.
  • Japanese Patent Publication No. 20,579/79 discloses a process for producing a high-purity maltose which comprises applying a starch sugar solution, containing glucose and maltose, to a column packed with an anion exchange resin of SO 3 2- - or SO.sub.
  • the present inventors have investigated processes for producing a high-purity maltose using a strongly-acidic cation exchange resin, more particularly, of alkali metal- or alkaline earth metal-form, instead of an anion exchange resin which has the above described disadvantages.
  • a high-purity maltose is easily obtainable by admitting a feed starch sugar solution with a maltose content of at least 70% and water to a column packed with a strongly-acidic cation exchange resin of alkali metal- or alkaline earth metal-form thereby fractionating the solution into a high-dextrin fraction, a high-dextrin, maltose fraction, a high-maltose fraction, a high-maltose.glucose fraction, and a high-glucose fraction (the terms "high-A fraction” and "high-A.B fraction” as used in the specification shall mean the eluted fractions rich in A, or rich in A but highly contaminated with B); and recovering the high-maltose fraction.
  • the drawing shows the elution pattern of the feed solution upon the fractionation into fractions A through E, i.e., the high-dextrin fraction, high-dextrin.maltose fraction, high-maltose fraction, high-maltose.glucose fraction, and high-glucose fraction, respectively.
  • the feed starch sugar solution usable in the present invention may be almost any substantially-ketose-free solution of aldoses derived from starch. Such a feed solution results in a high-maltose fraction; and with a maltose content of 90%, typically 93% or higher, in a high yield when subjected to the fractionation according to the present invention.
  • feed starch sugar solution may be a saccharified starch solution obtained by subjecting starch to the actions of starch-degrading enzymes, e.g., ⁇ - and ⁇ -amylases, and starch-debranching enzyme, or may be an aqueous solution of a commercially-available starch sugar product having a maltose content of at least 70%.
  • the strongly-acidic cation exchange resin of alkali metal- or alkaline earth metal-form usable in the invention may be, for example, one or more members of styrene-divinylbenzene copolymer resins bearing sulphonyl groups of alkali metal- or alkaline earth metal-form, such as Na + , K + , Ca 2+ , or Mg 2+ .
  • Commercially-available resins are, for example, "Dowex 50WX2", “Dowex 50WX4", and “Dowex 50WX8", products of Dow Chemical Company, Midland, Mich., U.S.A., "Amberlite CG-120", a product of Rohm & Haas Company, Philadelphia, Pa.
  • a resin with a nominal particle size of about 0.01-0.5 mm is packed in one or more columns.
  • the bed depth preferred in the invention is generally 7 m or longer. If two or more columns are used, they are cascaded to give a total bed depth of 7 m or longer.
  • any column can be used regardless of its material, size, and shape so far as the objectives of the invention can be attained therewith.
  • the column may be, for example, of glass, plastic or stainless steel, and its shape may be, for example, in cylindrical or square pillar form, but it should be designed to give the most effective laminar flow possible when the feed starch sugar solution is applied to the column packed with the resin.
  • One or more column(s) is packed with a strongly-acidic cation exchange resin of alkali metal- or alkaline earth metal-form, in an aqueous suspension, to give a total bed depth of 7 m or longer.
  • the feed starch sugar solution While keeping the temperature in the column(s) at 45°-85° C., the feed starch sugar solution, at a concentration of about 10-70 w/w %, in an amount of about 1-60 v/v % against the bed volume, is admitted into the column(s) and then charged upwards or downwards with water at a flow rate of about SV 0.1-2.0 to effect fractionation of the material starch sugar solution into a high-dextrin fraction, a high-dextrin.-maltose fraction, a high-maltose fraction, a high-maltose.glucose fraction, and a high-glucose fraction, in the given order.
  • the high maltose-fraction is then recovered.
  • the eluted fractions are generally collected in about 1-20 v/v % against the bed volume, they may be distributed automatically into the fractions.
  • the amount of water required for substantial fractionation of the feed starch sugar solution can be sharply reduced, and the maltose constituent in the solution can be recovered in higher purity, higher concentration, and higher yield.
  • the previously obtained high-dextrin.maltose fraction, the feed starch sugar solution, and the previoiusly obtained high-maltose.glucose fraction are applied successively to the column in the given order.
  • the high-maltose fraction thus obtained can be used intact, it may be, if necessary, treated further as follows.
  • the high-maltose fraction may be subjected to conventional purification steps, e.g., filtration, decolorization and/or deionization.
  • the purified product is, for example, concentrated to obtain a syrup, or crystallized to obtain a mascuit which may be spray-dried into crystalline powder, or separated into mother liquor and maltose crystals of much higher purity.
  • the high-purity maltose thus obtained is extremely useful in various applications, e.g., for production of food products or pharmaceuticals.
  • the feed starch sugar solutions used in this experiment were prepared from commercially-available starch sugar products as listed in Table I, products of Hayashibara Company, Limited, Okayama, Japan, by dissolving or diluting them in water to give respective concentrations of 45 w/w %.
  • each feed starch sugar solution listed in Table I was admitted to the column in an amount of 5 v/v % against the bed volume, and fractionated by charging 75° C. hot water at a flow rate of SV 0.4 through the column and the high-maltose fraction, with a maltose content of 93 % or higher, was recovered.
  • Table II The results are given in Table II.
  • a feed starch sugar solution was prepared by diluting "HM-75", trade name of a commercially-available starch sugar syrup with a maltose content of 76.8%, a product of Hayashibara Company, Limited, Okayama, Japan, in water to give a concentration of 45 w/w %.
  • XT-1022E (Na + ) a commercially-available strongly-acidic cation exchange resin of alkali metal-form, a product of Tokyo Chemical Industries, Kita-ku, Tokyo, Japan, in an aqueous suspension, was packed in four jacketted stainless steel columns, inside diameter, 5.4 cm, to give respective bed depths of 5 m, and the columns were cascaded to give a total bed depth of 20 m.
  • the feed starch sugar solution was admitted thereto in an amount of 5 v/v % against the bed volume, and then fractionated by charging 55° C. hot water at a flow rate of SV 0.13 through the columns and the high-maltose fraction, with a maltose content of 93% or higher, was recovered.
  • the high-maltose fraction contained 808.2 g maltose, and the yield was extremely high, i.e., 84.3%, against the maltose constituent in the feed starch sugar solution.
  • a feed starch sugar solution was prepared by dissolving "Sunmalt", a commercially-available starch sugar powder with a maltose content of 85.0%, Registered Trade Mark of Hayashibara Company, Limited, Okayama, Japan, in water to give a concentration of 60 w/w %.
  • the resin, used in Example 1, was converted into K + -form in the usual way and packed in a jacketted stainless steel column, inside diameter, 2.2 cm, to give a bed depth of 10 m.
  • the feed starch sugar solution was admitted thereto in an amount of 3 v/v % against the bed volume, and then fractionated by charging 60° C. hot water at a flow rate of SV 0.2 through the column and the high-maltose fraction, with a maltose content of 93% or higher, was recovered.
  • the high-maltose fraction contained 65.7 g maltose, and the yield was extremely high, i.e., 88.3% against the maltose constituent in the feed starch sugar solution.
  • a feed starch sugar solution was prepared by dissolving "Sunmalt", a commercially-available starch sugar powder with a maltose content of 85.0%, Registered Trade Mark of Hayashibara Company, Limited, Okayama, Japan, in water to give a concentration of 45 w/w %.
  • the feed starch sugar solution was applied thereto in an amount of 6.6 v/v % against the bed volume, and then fractionated by charging 75° C. hot water at a flow rate of SV 0.13 through the columns and the high-maltose fraction, with a maltose content of 93% or higher, was recovered.
  • the high-maltose fraction contained 913.7 g maltose, and the yield was extremely high, i.e., 87.1%, against the maltose constituent in the feed starch sugar solution.
  • the first fractionation was carried out as follows. Similarly as in Example 1, a feed starch sugar solution was applied to a column, and fractionated except that the feed starch sugar solution was applied to the column in an amount of 20 v/v % against the bed volume.
  • the elution pattern is given in the drawing, where Fractions A through E show a high-dextrin fraction, a high-dextrin.maltose fraction, a high-maltose fraction, a high-maltose.glucose fraction, and a high-glucose fraction respectively, and where the elution is effected in the given order.
  • Fraction C the high-maltose fraction
  • Fractions A and E were removed from the fractionation system.
  • the additional fractionation was carried out as follows. Fraction B, the feed starch sugar solution in an amount of about 10 v/v % against the bed volume, and Fraction D were admitted into the column successively in the given order, and the column then charged with 75° C. hot water, as in Example 3, to effect fractionation. The high maltose fractions, with a maltose content of 94%, were recovered. The additional fractionation was repeated up to 30 batches in total, and the averaged results per batch were calculated. On an average, one high-maltose fraction contained 1483 g maltose, and the yield was extremely high, i.e., 93.3%, against the maltose constituent in the feed starch sugar solution.
  • a feed starch sugar solution was prepared by dissolving "Maltose H", trade name of a commercially-available starch sugar powder with a maltose content of 91.5%, a product of Hayashibara Company, Limited, Okayama, Japan, in water to give a concentration of 45 w/w %.
  • Amberlite CG-120 (Ca 2+ ) a commercially-available strongly-acidic cation exchange resin of alkaline earth metal-form, a product of Rohm & Haas Company, Philadelphia, Pa., U.S.A., was packed in fresh columns of the same material and dimensions as used in Example 1 to give a total bed depth of 10 m.
  • Example 4 a dual-stage fractionation was carried out.
  • the first fractionation was carried out as follows. While keeping the temperature in the columns at 80° C., the feed starch sugar solution was applied thereto in an amount of 20 v/v % against the bed volume, and then fractinated by charging 80° C. hot water at a flow rate of SV 0.6 through the columns to obtain a similar elution pattern as in Example 4. Similarly as in Example 4, Fraction C, the high-maltose fraction, was harvested, and Fractions A and E were removed from the fractionation system.
  • Fraction B the feed starch sugar solution in an amount of 10 v/v % against the bed volume, and Fraction D, were admitted into the column successively in the given order, and the column then charged with 80° C. hot water at a flow rate of SV 0.6 to effect fractionation.
  • the resultant high-maltose fractions with a maltose content of 96% or higher, was recovered.
  • the additional fractionation was repeated up to 100 batches in total, and the averaged results per batch were calculated. On an average, one high-maltose fraction contained 1084 g maltose, and the yield was extremely high, i.e., 95%, against the maltose constituent in the feed starch sugar solution.

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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US07/202,249 1981-08-03 1988-06-06 Process for producing a high-purity maltose Expired - Lifetime USRE33047E (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP56121725A JPS5823799A (ja) 1981-08-03 1981-08-03 高純度マルト−スの製造方法
JP56-121725 1981-08-03

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US06/402,823 Reissue US4487198A (en) 1982-07-28 1982-07-28 Process for producing a high-purity maltose

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US (1) USRE33047E (de)
JP (1) JPS5823799A (de)
CA (1) CA1215361A (de)
DE (1) DE3228838A1 (de)
FR (1) FR2510581B1 (de)
GB (1) GB2106912B (de)

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US5773282A (en) * 1994-10-01 1998-06-30 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Recombinant thermostable enzyme for converting maltose into trehalose from Thermus aquaticus
US6066477A (en) 1996-11-08 2000-05-23 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Method of producing glucosylated saccharides with kojibiose phosphorylase
US6436678B2 (en) 2000-02-28 2002-08-20 Grain Processing Corporation High purity maltose process and products
US20030021866A1 (en) * 2001-07-24 2003-01-30 Grain Processing Corporation Method for making wine
US20050009017A1 (en) * 2000-05-22 2005-01-13 Michio Kubota Alpha-isomaltosyltransferase, process for producing the same and use thereof
US20060188626A1 (en) * 2003-07-18 2006-08-24 Stouffs Robert H Process for preparing maltitol enriched products
US7186535B1 (en) 1998-09-11 2007-03-06 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
US7241606B2 (en) 2000-08-01 2007-07-10 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo α-Isomaltosylglucosaccharide synthase, process for producing the same and use thereof
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JP7441178B2 (ja) 2018-12-13 2024-02-29 株式会社林原 シクロイソマルトテトラオース、シクロイソマルトテトラオース生成酵素とそれらの製造方法並びに用途

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GB1426976A (en) * 1973-06-05 1976-03-03 Sanmatsu Kogyo Co Method for the production of maltose
JPS5186143A (en) * 1975-01-21 1976-07-28 Tokai Togyo Kk Katono seizohoho
US4022637A (en) * 1976-02-23 1977-05-10 Standard Brands Incorporated Method for separation of water soluble carbohydrates
JPS5246290A (en) * 1976-09-17 1977-04-12 Erepon Kakoki Kk Ph control system of waste fluid
US4294623A (en) * 1978-12-07 1981-10-13 Meiji Seika Kaisha, Ltd. Method of producing high purity maltose
JPS5585395A (en) * 1978-12-21 1980-06-27 Nippon Shiryo Kogyo Kk Preparation of crystalline maltose
US4384898A (en) * 1980-07-31 1983-05-24 Nihon Shokuhin Kako Co., Ltd. Process for producing cyclodextrins
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US6087146A (en) 1994-10-01 2000-07-11 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Recombinant thermostable enzyme for converting maltose into trehalose
US5773282A (en) * 1994-10-01 1998-06-30 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Recombinant thermostable enzyme for converting maltose into trehalose from Thermus aquaticus
US6066477A (en) 1996-11-08 2000-05-23 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Method of producing glucosylated saccharides with kojibiose phosphorylase
US6204377B1 (en) 1996-11-08 2001-03-20 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Kojibiose phosphorylase glucosyl-saccharides produced by transglycosylation
US20070218529A1 (en) * 1998-09-11 2007-09-20 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
US7582463B2 (en) 1998-09-11 2009-09-01 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
US7575900B2 (en) 1998-09-11 2009-08-18 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
US20070281346A1 (en) * 1998-09-11 2007-12-06 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
US7186535B1 (en) 1998-09-11 2007-03-06 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes
US6436678B2 (en) 2000-02-28 2002-08-20 Grain Processing Corporation High purity maltose process and products
US6670155B2 (en) 2000-02-28 2003-12-30 Grain Processing Corporation Process for preparing dextrins
US7192746B2 (en) 2000-05-22 2007-03-20 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo α-Isomaltosyltransferase, process for producing the same and use thereof
US20050009017A1 (en) * 2000-05-22 2005-01-13 Michio Kubota Alpha-isomaltosyltransferase, process for producing the same and use thereof
US7241606B2 (en) 2000-08-01 2007-07-10 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo α-Isomaltosylglucosaccharide synthase, process for producing the same and use thereof
US7718404B2 (en) 2000-08-01 2010-05-18 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo α-isomaltosylglucosaccharide-forming enzyme, process and uses of the same
US7811797B2 (en) 2000-08-01 2010-10-12 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo α-isomaltosylglucosaccharide-forming enzyme, process and uses of the same
US7709230B2 (en) 2001-04-27 2010-05-04 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Process for producing isomaltose and uses thereof
US20030021866A1 (en) * 2001-07-24 2003-01-30 Grain Processing Corporation Method for making wine
US20060188626A1 (en) * 2003-07-18 2006-08-24 Stouffs Robert H Process for preparing maltitol enriched products
US7935190B2 (en) 2003-07-18 2011-05-03 Cargill, Incorporated Process for preparing maltitol enriched products

Also Published As

Publication number Publication date
JPS6251120B2 (de) 1987-10-28
JPS5823799A (ja) 1983-02-12
DE3228838A1 (de) 1983-03-24
CA1215361A (en) 1986-12-16
GB2106912A (en) 1983-04-20
GB2106912B (en) 1986-03-12
DE3228838C2 (de) 1990-03-29
FR2510581A1 (fr) 1983-02-04
FR2510581B1 (fr) 1987-10-16

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